tissue culture LP by 8034e1

VIEWS: 28 PAGES: 4

									Amber Minix
AS&D 4203
Agriscience 362: Horticultural Plant Production
Unit: Asexual Propagation
Lesson: Tissue Culture

LESSON PURPOSE:
    The purpose of this lesson is to provide students with the information
    necessary to determine the role of tissue culture in the asexual
    propagation of plants.

OBJECTIVES:
    Upon completion of the lesson the student(s) will be able to:
          1. define the process of tissue culture.
          2. list all components of the tissue culture process in order.
          3. discuss the advantages and disadvantages of tissue culture.

TEKS: 119.24 (2)(b)

TAKS: English and Language Arts
      English I & II: 1(6)(B)(E) 1(7)(F)(E)(G) 1 (8)(B)
      Mathematics
      Algebra and Geometry: 1A(b)(1)(A) 1A(b)(1)(B) 1A(b)(1)(E) 9(8.11)(B)
         10(8.14) (A) 10(8.15)(A) 10(8.16)(B)
         Science
         Biology: 1(1)(A) 1(2)(A) 1(2)(B) 1(2)(C) 1(2)(D) 2(4)(B) 2(8)(C) 2(10)(A)
         2(10)(B) 3(9)(D) 3(13)(A)
         I Physics and Chemistry: 4(9)(A) 4(9)(B)



FOCUS:                                                               NOTES:
Inform students that we will be discussing the process of
tissue culture, including:

   1.   the history
   2.   the materials required
   3.   the procedure
   4.   the advantages and disadvantages



Ask students the following related questions:
   1. Why might we want to reproduce many plants at             Commercial
       once?                                                    Production/
   2. Why might it be important to use tissue culture?          Nursery
LESSON CONTENT:
Define Term(s):
   1. Tissue Culture- the production of new plantlets from
       the tissue of an actively growing plant
   2. Callus- the soft tissue that forms over a cut
   3. Explant- the plant pieces removed to be cultured


Discuss the history of tissue culture.
    Discovered in 1965
    Micropropagation
    Beginning of tissue culture

Discuss why tissue culture is used.

   1. REGENERATION PROBLEMS
         Poor germination
   2. DESIRABLE TRAITS
         Disease-resistance
         High productivity
   3. GENDER OF IMPORTANCE
         Male- Asparagus
         Female- Papaya
         May effect the sales of a specific plant

Discuss where tissue culture is used.
   1. COMMERCIAL PRODUCTION
        Examples: chrysanthemum, gladiolas, etc.
   2. CROP PRODUCTION
        Examples: sugar cane, tapioca, potatoes

Discuss what is required:
    Sanitary conditions
          o lab
    Sterile media
          o Four components: nutrients, sugars, vitamins,
              hormones
          o Can be made at home                                 Show Plant
          o Jell-O-like consistency                             Examples: shoot
    Test tube                                                  tip, leaf section,
    Plant tissue                                               meristem, node
          o Any vegetative part


Discuss the Procedure of Tissue Culture:
   The tissue culture procedure includes all of the following
steps to produce plantlets:                                     Discuss all
    1. Prepare media                                            material to be
    2. Sterilize everything                                     sanitized
    3. Place media in test tube
   4.   Remove tissue from plant                                Discuss Explant
   5.   Sanitize tissue sample
   6.   Place tissue sample on media in teat tube
   7.   Cap or seal test tube
   8.   Place in a warm, well-lighted area
   9.   Plantlets should begin to form in 4-6 weeks

Students discuss Advantages and Disadvantages of the
tissue culture process in groups.

GUIDED PRACTICE:
Students will put the steps for tissue culture in order using   Check for the
the packages provided. Each package contains the NINE           correct order.
STEPS from above, cut into individual strips, that the
students will put in the correct order starting with number
one.



INDEPENDENT PRACTICE:                                           1.
Students will answer the following questions independently:     Micropropagation
                                                                2. this answer
   1. What is another name for tissue culture?                  will depend
   2. Define tissue culture in your own words.                  3. refer to slide
   3. List one advantage and one disadvantage of tissue         10
      culture.                                                  4. sanitary lab,
   4. What four materials are required for the tissue culture   media, test tube,
      process?                                                  plant tissue


CONCLUSION:
Teacher reviews tissue culture with students:
   1. What is tissue culture?
   2. Where is tissue culture used?
   3. What materials are required for the process?


LIST OF MATERIALS:
    PowerPoint Presentation
    Tissue Culture Handout
    Plant

REFERENCES:
   IMS: Horticulture Plant Production 8930-D
   Plant Tissue Culture: www.accessexcellence.org/LC/ST/st2bgplant.html
   Georgia Agriculture Education Curriculum: Course 01.461 Unit 5 Lesson 2

								
To top