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					                    Format for submitting Annual Progress Report
                             (April 2010 – March 2011)

COMPONENT: IV

1. Sub-project title:“Novel biotechnological processes for production of high value
    products from rice straw and bagasse

2. Principal Investigator: Dr.BS Chadha

3. Lead Institute: Guru Nanak Dev University, Amritsar

4. Co-partners (Name and address): Central Institute of Post Harvest Engineering and
   Technology, Ludhiana and Indian Institute of Technology, Delhi

5. Duration of the Sub-project:
   Date of commencement: January 14, 2009
   Date of completion: March 31, 2012
   Extension (if any): might need six months extension to fulfill all objectives

6. Total budget of sub-project:
   Change in budget (if any): Rs 326.75 lakhs

7. Research Achievements/Innovations:
   (List five in bullet form, not more than 3 lines each)
   a) For Period of report i.e., April, 2010 to March, 2011


  Pellets made out with rice straw or bagasse without addition of binders at a moisture
   content of 20-25% showed good textural attributes. Pretreatment using alkali has been
   optimized for both rice straw and bagasse.

  Biosurfactant assisted enzymatic hydrolysis of pretreated alkali rice straw improved
   hydrolysis efficiency thereby presenting an opportunity to go for higher solid loading.

  Soft carbohydrate obtained by simple enzymatic hydrolysis of untreated rice straw and
   especially bagasse holds commercial potential for use in food processing industry.

  Hydrolysis of untreated well ground bagasse using crude enzyme resulted in gluctose and
   fructose concentrations of 23 g/l and 18.4 g/l. Residual biomass was used for cellulase
   production.

  A low cost unit for membrane concentration of enzymes has been designed, developed
   and is being tested. The desired enzymes could be concentrated five folds in two hours.


    b) From inception to March, 2011
      Novel cellulase producing fungal strains have been isolated characterized at molecular
       level (amplified ITS region of DNA) and functional level on the basis of diverse
       endoglucanases, cellobiohydrolases and β glucosidases separated by IEF and PAGE.

      Metabolic footprint profiling of culture extracts of Aspergillus sp. growing in presence of
       corncob showed sequential induction of endoglucanase isoforms was associated with the
       synthesis/formation of distinct metabolites (monosaccharides/oligosaccharides).

      Pretreatment using alkali has been optimized for both rice straw and bagasse.
       Biosurfactant assisted enzymatic hydrolysis of pretreated alkali rice straw improved
       hydrolysis efficiency thereby presenting an opportunity to go for higher solid loading.

      Soft carbohydrate obtained by simple enzymatic hydrolysis of untreated rice straw and
       especially bagasse holds commercial potential for use in food processing industry.

      A low cost unit for membrane concentration of enzymes has been designed, developed
       and tested. The membrane based system for nanofiltration for separation of
       oligosaccharides from monosaccharides has been designed and is being tested.

   8. Salient achievements/innovations:
      (List two achievements with half page write-up and good photographs)

       Secretome analysis of Aspergillus fumigatus was being reported for the first time and the
       biosurfactant produced by a novel strain of Trichoderma asperlum found to enhance the
       saccharification of alkali treated rice straw. 2D gel electrophoresis analysis of the crude
       enzyme obtained from an isolate of Aspergillus fumigates was done under standard
       cultural conditions as well as with the use of specific inducers. Enzymes belonging to
       glycoside hydrolase family have been identified and characterized. Four β- glucosidases
       and four distinct CBH/EGI active protein spots have been identified. The 2DE profiling
       of the secretome of Aspergillus fumigatus carried out for the first time. Four β-
       glucosidases and four distinct CBH/EGI active protein spots were identified.




                     A                                            B
Zymogram for localization of A: CBH/EG1 and B: β- glucosidases in 2DE gels
Laboratory scale cross flow filtration (CFF) for Ultrafiltration (UF) & Nanofiltration (NF) unit
has been developed which has certain unique features such as low hold up volume, capacity to
operate at low to high cross flow velocity and pressure up to 225 psi, low processing volume and
ease of scale-up. The system has been designed for separation of oligosaccharides such as xylo-
oligosaccharides from monosaccharides. The oligosachharides are high value products having a
tremendous potential in food and pharmaceutical industry.




Laboratory scale cross flow filtration (CFF) for Ultrafiltration (UF) & Nanofiltration (NF)
                                           unit
   9. Financial Management
      Statement of budget released and utilization (since inception):          (Rs. lakh)
                                          Total         Fund Released        Fund utilized
         Sl.       Lead centre/
                                       Sanctioned        upto March           upto March
         No.       Co-partners
                                         Budget             2011                 2011
        1.      GNDU, Amritsar            154.91             112.68             104.88
        2.      CIPHET, Ludhiana           99.71             83.80               77.04
        3.      IIT, Delhi                 72.12             41.53               44.95


   10. Meetings/seminars/trainings etc. organised :NIL
                                   Details of
             Organizer                                                    No. of
                                   Meetings/           Duration
             (Name &                                                    Personnel   Budget
                                   Seminars/          (From-To)
             Address)                                                    trained
                                 Trainings, etc.
11. Participation in Conference/meetings/seminars/trainings/Radio talks etc.
                                        Details of
            Participant                                        Duration
                               Meetings/Seminars/Trainings                                 Budget
       (Name & Address)                                       (From-To)
                                     /Radio talk, etc.
       Dr. Harinder Singh        Delivered an invited talk on           December 14-         0.15
         Oberoi, Senior          “Evaluation of soft                      17, 2010          lakhs
       Scientist, CIPHET,        carbohydrates in hydrolysis of
            Ludhiana             alkali-treated rice straw and
                                 bagasse”.


12. Cumulative list of Publications:
    (Research Papers in peer reviewed journals, in standard format)
                  Title of the papers & authors                       Name of Journal, Vol., etc.
     Sharma M, Soni R, Nazir A, Oberoi HS and Chadha               Applied Biochemistry and
     BS (2010) Evaluation of glycosyl hydrolases in the            Biotechnology, 163, 577-591.
     secretome of Aspergillus fumigatus and
     saccharification of alkali-treated rice straw

     Babbar N, Oberoi HS, Dhaliwal SS, Sandhu SK, Kaur             Under review with Process
     U and Bhargav VK. Comparison of simultaneous                  Biochemistry
     saccharification and fermentation and separate
     hydrolysis and fermentation processes for ethanol
     production from alkali- treated rice straw using newly
     isolated thermotolerant strain of Pichia kudriavzevii.

13. Media products developed/disseminated: NIL
          CD, Bulletins, Brochures, etc.
                                                     No. of copies                Distribution
                (Year wise); title



14. Cumulative list of Patents (if any): NIL
              Patent developed by                   Patent details
                                                                                  Application No.
               (Name & Address)                    (Granted/Filed)
     Professor GP Agarwal, Department of        Provisional patent filing
     Biochemical Engineering and                under process
     Biotechnology, Indian Institute of
     Technology, Delhi




15. Knowledge products developed: (Applicable to component 1 only)

16. Assets Generated:
    Equipments/Vehicles/Research facilities created from starting to March 2011
                               Equipment                                                        Amount
                Lead                                    Vehicle
     Sl.                        (Make &                           Research        Date of         spent
               centre/                                 (Make &
     No.                      Model)[ Above                       facilities     purchase     (Rs. in lakhs)
             Co-partners                                Model)
                               5.0 lakhs]
     1.                         Fluorescent               -       Analytical     April 2010       13.50
            GNDU
                                plate reader
            Amritsar
                              (BMG labtech)
                                Akta prime                -       Analytical     May 2010          6.0
                                  protein
                                purification
                                  system
                               HPLC Ultimate              -       Analytical     June 2010         17.0
                                  (Dionex
                                Corporation,
                                   USA)
     2.                        HPLC Ultimate              -       Analytical     May 2010       18.0 lakhs
            CIPHET,                (Dionex
            Ludhiana            Corporation,
                                     USA)
     3.                               Gas                 -       Analytical     May 2010       9.0 lakhs
                               chromatograph
            IIT Delhi
                              (Agilent, Model
                                    A7190)
                              UF/NF cross                 -        Lab scale     June 2010      9.0 lakhs
                              flow membrane
                              modules
                              (Nishotech,
                              Mumbai and
                              GE CEPA
                              filtration unit)



17. Infrastructure facilities developed:
     Sub-Project Leader       Infrastructure       Numbers/Size/
                                                                                    Amount (Rs.)
        and Partners            developed            Capacity
     CIPHET, Ludhiana        Fume hood            01                    85,000
                             installation
                             Installation of      01                    20,000
                             air curtain in the
                             laboratory

18. Revenue generated (cumulative upto March, 2011): NIL
    (From sale of farm produce, patents, commercialization, trainings, etc)
      Sub-Project Leader
                                Source of revenue                  Amount (Rs.)
         and Partners



 19. Personnel involved in sub-project
       Leader and                    Regular                         Contractual
        Partners
                                          Pr.
        (Name &        Sc.      Sr. Sc              Tech.    RA    SRF        TA   Others
                                          Sc.
        Address)
     GNDU Amritsar    01        02       01        NIL      NIL    03     NIL
     CIPHET,          NIL       01       NIL       NIL      NIL    01     NIL
     Ludhiana
     IIT Delhi        NIL       NIL      01        NIL      NIL    01     NIL

 20. Honour and Awards:NIL
      Sub-Project Leader          Name of                      Honour/Award
         and Partners            personnel                      (with date)




 21. Linkages and collaborations (specific to project activities)
        Sub-Project Leader
                                                Linkages developed
           and Partners
     CIPHET                     CIPHET has developed a strong linkage with GADVASU,
                                Ludhiana and NIPER, Mohali for future collaborative research
                                work

 22. Status of employment generation (man-days/year)
      Lead centre/    Employment generation              Employment generation during
      Co-partners       upto March 2011                    April 2010 to March 2011
     GNDU,            Three SRFs are working in                         NIL
     Amritsar         the project since inception
     CIPHET,          One SRF is associated with                        NIL
     Ludhiana          the project since project
                           commencement
     IIT, Delhi       One SRF is working in the                         NIL
                      project since beginning of
                              the project




Signature of Consortia Leader                                Signature of Principal Investigator
   (Head of Organization)

				
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