DNA Extraction - PowerPoint by 4SJ1VCK7

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									                DNA Extraction

   Common molecular biology lab procedure

   Why extract DNA ?
       To study DNA structure and function
       Paternity testing/Genetic testing
       Forensics
               Key Players


   Cells – DNA stored in nucleus

   Salt (NaCl)
      DNA has a negative charge

      Na+ neutralizes DNA’s charge
                      Key Players


   Detergent


        dissolves lipids
         of membrane
                  Remove Proteins


   Enzyme added

        Protease –
         breaks down the
         proteins

        “cuts” histones
         from DNA


                           http://learn.genetics.utah.edu/units/activities/extraction/enzyme
                  Precipitate DNA


   Ethanol added

        DNA is insoluble in
         ethanol (clumps
         together)

                                                                                               DNA

                                                                                            ethanol
                                                                               cells/soap/salt
                               http://learn.genetics.utah.edu/units/activities/extraction

								
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