"molecular cellular biology 4h 2003 04 paper 5"
Tuesday 18th May 2004 9.30 am - 12.30 pm University of Glasgow Degree of B.Sc (Honours) and M.Sci. Molecular & Cellular Biology 4H Biotechnology 4H Molecular & Cellular Biology 4M Biotechnology 4M (With Work Placement) Paper 5 (General Paper) Answer THREE questions, each in a separate book. 1 Very recently a type of protein splicing (cutting and pasting of peptides) has been described in cells of the human immune system. Discuss the implications that this might have for the coding potential of the human genome and complexity of the proteome, if the process existed alongside RNA splicing and DNA recombination. 2 New and emerging viruses pose a major threat to human kind. Discuss. 3 Write an essay on the metabolic importance of CO2-fixing enzymes. 4 Evaluate the available methods for production of protein drugs, such as insulin, interferons and erythropoietin, from cloned DNA. 5 Write an account of protein modification as a way of regulating the quantity of active protein in the cell. 6 Using specific examples of research areas, discuss the relative merits and/or limitations of utilizing invertebrate model organisms for biomedical research. 7 Ribonucleoprotein complexes occur regularly within many cells, perhaps the best known being the ribosome and the spliceosome. Give an overview of what is known about the compositions and functions of such complexes, considering especially whether the RNA or protein parts are functionally the most important molecules. 8 Disease-causing bacteria that are resistant to multiple antibiotics are now a major health problem. Discuss the biological processes that lead to formation of these multidrug-resistant bacterial strains. 9 The protein filaments of eukaryotic cells are frequently referred to as “the cytoskeleton”. How appropriate is this? 10 Why is the ability to grow animal tissue cells in culture such an important technique in current biological research? CONTINUED OVERLEAF/ Molecular & Cellular Biology/Biotechnology-4H/4M, May 2004, Paper 5 Page 1 of 2 11 The Glasgow Molecular & Cellular Biology Degree would be an ideal intercalated B.Sc. for a medical student. Discuss. 12 Co-ordination of the various steps involved in gene expression is a major feature of eukaryotic cells. Discuss. 13 Design a series of experiments that would enable you to identify genes that play a major role in the visual system in Drosophila melanogaster. 14 The human genome contains thousands of completely novel genes. Evaluate the role of reverse genetics in aiding our understanding of these genes. What other approaches are possible? 15 You are studying a novel RNA virus and suspect that one of its open reading frames, ORF7, may encode a protein which interferes with the RNA silencing (RNAi) machinery of the cell. How would you investigate this theory? END OF EXAMINATION Molecular & Cellular Biology/Biotechnology-4H/4M, May 2004, Paper 5 Page 2 of 2