Je sousign� Rita HADATHI avoir re�u la somme de 1 500 000 LL du

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					                                       Administrative Information
                                            ‫المعلومات االدارية‬

                  :‫المرجع‬                                    Project Title - )‫عنوان المشروع (عربي وأجنبي‬
‫( بما فيها مركبات السم‬endémique Montevipera bornmuelleri(‫دراسة بيئية وفيزيولوجية ألفعى لبنانية جبلية‬
Etude écologique et physiologique d'une espèce de vipère endémique du Mont-Liban:
Montevipera bornmuelleri, composition du venin et ses effets.

An ecological and physiological study of the mountain viper Montivipera bornmuelleri
including venom composition and effects.

                                                               Principal Investigator - ‫الباحث الرئيسي‬
   ‫رقم الهاتف‬     ‫العنوان االلكتروني‬       ‫العنوان‬         ‫الوظيفية‬         ‫المؤسسة‬           ‫االسم‬
  Telephone           e-mail              Address           Post          Institution        Name
  03 14 17 93       sdbloquet           U.L. Faculty      Professor        Lebanese          Souad
  04 91 20 24            of Sciences                       University        HRAOUI
                                        B.P. 90656                        Faculty of       BLOQUET
                                         Jdeidet EL                        Sciences

                                                                      Co-Workers - ‫الباحثون المشاركون‬
                                               ‫العنوان االلكتروني‬            ‫المؤسسة‬               ‫االسم‬
                                                         e-mail          Institution              Name
                 Walid                        USEK           Dr Walid
                                                            Sciences faculty     Hleihel
                                      AUB            Dr Ryiad
                                                               Faculty of        Sadek
      Dr Elias Baydoun from AUB doesn’t do his part of work, the biochemical part was done
       by Dr Ziad Fajloun From UL (Lebanese University, Faculty of Sciences section III).
               his electronic address : zfajloun@                        cf. letter
               presented to CNRS ( the number of enregistration: 7881 on 14/07/2010

                    2 years + 1year(prolongation) = 3years            : Duration -‫المدة التعاقدية للمشروع‬
                                   Scientific Information

                                        ‫المعلومات العلم ّة‬
                                                                         Objectives - ‫الهدف‬

Learning about this endangered species (montevipera bornmuelleri) of Lebanon, its
geographic and habitat distribution and contributing to its conservation in addition to
learning about its vulnerability to future climatic changes and its interaction with the
natural as well as the human environment.

The study is concentrate on a mountain viper found at high altitudes in Lebanese
Mountains in terms of geographic distribution. Biochemical studies done to found
application for some components in medical domain and to found ways to reduce potential
envenomation and devising treatments based on studying the effects of snake’s venom on
different tissues:
   -   The biochemical study will concentrate on the composition of the venom and the
       time required for production of venom.
   -   The toxic effects will be investigated in vivo on laboratory animals in terms of its
       effect on physiological functions of these animals especially in relation to cardiac
       muscles, blood vessels and nervous system.
   -   It is possible to use the results to help in treatment of detoxification and the
       production of anti-venom and in other medical applications especially in relation to
       various therapeutic uses.
                                                            Achievements –‫أالنجازات المحققة‬
In this study:
   -   The ecological part, (Field study) biogeography study, distribution, density,
       habitat, biological activities (reproduction, alimentary regime, threaten, etc…)
       for 2 regions in Lebanon: Jabal Makmel et Jabal sannine, was studied.

   -   Biochemical composition of the venom (laboratory study) is done and need more
       time to be completed. The results obtained are preliminary.

For those 2 parts of the project, results were presented at the congress in Morocco in
May, 2011. And a paper was presented to the CNRS            journal for publication, and

   -   For the third part, the physiological study of the venom, give interesting results
       not yet published. We present in this report the results we have get. This
       laboratory study asked more time to be finished and completed.

                                                                     Perspectives - ‫آفاق البحث‬

   -   No research is done to study venom and its application in Lebanon. This study,
       will found applications to medical domain, like a therapeutic application for some

   -   A karyotype and molecular study let us to recognize the phylogeny of this viper to
       confer for this to study done in another species of vipers (Wuster and al., 2007;
       Young, 2007; Murphy and al. 2007; Stumpel and joger, 2007) and to identify its
       relation with others species of the region.

   -   It is possible to use the results to help in treatment of detoxification and the
       production of anti-venom.
                        Publications & Communications - ‫المنشورات والمساهمات في المؤتمرات‬
    1 : One paper entitled : An ecological study of the Lebanon Mountain viper,
    Montevipera bornmuelleri (Werner, 1898) with apreliminary biochemical
    characterization of its venom.
    Is accepted to be published In : « lebanease Sciences Journal » (of lebanese CNRS).

    2 : tow papers were presented to the Congress CMH2 of Morocco (Marrakech, on 23 of
    May, 2011) :

-   One oral presentation on a biodiversity of this viper entitled: Etude écologique d’une
    vipère endémique des montagnes du Liban: Montevipera bornmuelleri

-   One poster on biochimerstry study of the venom entitled: LC-ESI-MS analysis and
    biological properties of the crude venom of Montevipera bornmuelleri

-   NB : 2 others papers are in redaction and will be presented for bublisher in a specialised
    scientific revues

-   one concerning the biochimestry results

-   the other one concerning the resultes of physiological applications in vivo

                                                               Abstract - ‫موجز عن نتائج البحث‬

-   This study concentrates on the Lebanon mountain viper Montivipera bornmuelleri
    endemic to the Lebanese mountains, in terms of ecology, geographic distribution
    and preliminary biochemical characterization of its venom. Here, we show that
    Montivipera bornmuelleri lives in habitats dominated by a variety of mountain
    plant species at altitudes ranging from 1900 m to 2200 m. This viper exists in
    association with endemic lacertid lizards in the Oyoun Simane area of Sannine
    mountain and with other snakes (such as Platyceps najadum and Hemorrhois
    ravergieri) in the Makmel (Bcharre) mountain.

-   Preliminary analysis of this viper’s venom was conducted using Liquid
    Chromatography coupled to Electrospray Ionisation Mass Spectrometry (LC-ESI-
    MS). This analysis revealed the presence of some bioactive molecules in the crude
    product. This work constitutes a preliminary study of a research project involving
       the extraction and characterization of bioactive molecules and potential
       therapeutic uses of the venom.

   -   The physiological study was conduct on the lethality by intramuscular injection of
       the crude venom. The LD50 is the dose that causes the death of 50% of animals
       within 24 h. The determination of the hemorrhagic, necrotic and procoagulant
       activities give interesting results. The minimum hemorrhagic dose (MHD) was
       determined. The minimum hemorrhagic dose (MHD) is 200µg. The venom
       exhibited strong hemorrhagic and necrotic activities. The estimation of MHD and
       (MND) minimum necrotic dose, help to determinate the doses of antivenom that
       neutralize the hemorrhagic and necrotic effects.

Keywords: Lebanon viper, Montivipera bornmuelleri, ecology, geographic distribution,
            venom analysis, Liquid Chromatography, Electrospray Ionisation Mass
            Spectrometry, lethal dose, minimum hemorrhagic dose (MHD), minimum
            necrotic dose (MND).

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