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Microbial Diversity

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					Microbial Diversity

   Project leader: Daniela Goltsman
   Jose Villanueva, Victoria Partida,
         Katherine Cantarero
         Table Of Contents. . .
•   Introduction
•   Inoculation
•   Isolation
•   FISH
•   Results
•   Conclusion
              Background
• Why are microorganisms important?
• How are microorganisms studied today?
  – Aseptic technique
  – Dyes
• What was the goal of our experiment?
  – To study microbial diversity around our
    environment
Diversity of life
           Sampling Sites

• Our samples:
  – Jose’s bathroom
  – UC Berkeley’s Strawberry Creek
  – Foothill Rec. Room Keyboard
  – Katherine’s Dorm
• Why did we choose these samples?
Which location
do YOU think
is the diverse
or “dirtiest” ?
       What is inoculation?
• The introduction of microorganisms into
  any media.
• For us this process took about three days
  to complete.
• We incubated at 37 00 C
Jose’s Bathroom
Katherine’s dorm
Foothill Rec. room keyboard
UC Berkeley’s Strawberry Creek
               Isolation
• Taking one colony or “population” of one
  type of microorganism and making those
  populations reproduce and grow.
Isolate from Jose’s bathroom
Isolate from rec. room keyboard
UC Berkeley’s Strawberry Creek
          What is FISH?
• FISH: Fluorescent In Situ Hybridization.
• This is a powerful technique that can be
  used to evaluate and analyze the
  presence of organisms in their community,
  their phylogeny, morphology, and number
  by targeting the 16S rRNA.
              FISH probes
• What is a probe?
  – Short sequence of DNA that hybridizes
    (binds) to a segment of the 16S rRNA.
  – Each probe carries a different color dye.
• Our dyes:
  – 4 different probes 3 different colors
  – Blue- Gama proteobacteria & Eukaryotic cells
  – Green- Universal bacteria
  – Red- Beta proteobacteria
Sample 1 LAPTOP. Green: bacteria, red:
         beta-proteobacteria.
Sample 2: STRAWBERRY CREEK Green: universal
bacteria, red: beta-proteobacteria, soft blue: gama-
     proteobacteria, bright blue: eukaryotes.
 Sample 3:KATHERINE’S ROOM Red:
beta-proteobacteria, blue: eukaryotes.
Sample 4. Green: universal bacteria,
         blue: eukaryotes
            Conclusion
In conclusion, we learned that:

– Microorganisms are EVERYWHERE!
– There are many kinds of bacteria that take on
  many shapes and sizes.
– Keyboards and bathrooms are the most
  diverse
–
Thank you!

				
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posted:7/23/2012
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