Lab Report Rubric - DOC by HC120707014440

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									Biology                                                            Drug Lab Techniques

PROJECT
OBSERVING THE EFFECT OF
DRUGS ON THE NERVOUS SYSTEM AND HEART RATE

Techniques and Background Information to help you plan your experiment.

Drugs known as stimulants increase nerve cell activity, thereby increasing movement, heart
rate, breathing, etc. Other drugs, known as depressants, slow nerve cell activity and decrease
the rate of metabolism. Heart rate is a very reliable indicator of increased or decreased nerve
activity.

Most drugs affect the nervous system of animals the same way. To examine the effects of drugs
you’ll use a small crustacean, Daphnia, which is related to shrimp and lobsters. They are also
known as water fleas and live in fresh water lakes and streams.

Below is a description of how to prepare Daphnia for observation. You’ll use the techniques to
establish a baseline (normal heart rate for Daphnia), and to test the effects of drug solutions.

SAFETY
Handle all equipment and drug samples carefully. Wash your hands thoroughly at the end of lab
classes. FOLLOW VERBAL and WRITTEN instructions for use of materials. Treat the Daphnia and
materials with respect.
                                                                                                   I only live
                                                                                                   about 5 weeks
MATERIALS:                                                                                         so be cool!

Daphnia
Petroleum jelly
Microscope slides
Small rubber washers
Coverslips
Compound microscope
Clock with second hand
Medicine dropper bottles for drug solutions
Eye dropper for collecting Daphnia
Ringer’s Solution
5ml medicine cup
Data table


PROCEDURE:

Collecting control data

1. Create an observation chamber. Using your finger rub a small amount of Vaseline on one of
the flat sides of a rubber washer. Place the washer, grease side down, on a glass microscope
slide, and gently press to form a tight seal. Add 2 drops of Ringer’s Solution.

Your set up should look like this:
                                                      Rubber washer

                                                                              Slide
Biology                                                              Drug Lab Techniques

2. Using a clean medicine or eye dropper, collect one Daphnia in fluid from the stock culture.
Wait until the daphnia swims near the opening of the dropper and gently squeeze the Daphnia
into the observation chamber. Add the coverslip to prevent evaporation of liquid. Place the slide
on the microscope and observe under low power. Make sure the light source is on low power to
avoid “cooking” the daphnia.

                                                                  eye
                        Heart
                                                               Antenna
                Intestine


            Egg pouch                                  Feet w/
                                                       Gills




3. To collect CONTROL data, count the Daphnia’s heart beats for 10 seconds. Record this
number under Trial 1 in your data table. Turn off the microscope light, and wait 20 seconds.
Record the count for Trials 2 and 3, always turning off the light and waiting 20 seconds between
each 10 second count.

4. Make notes of the daphnia’s activity (swimming rapidly, moving slowly, spastic twitching, etc.)

5. When done, carefully use an eye dropper to transfer the daphnia to a miniature medicine cup
containing 5 ml Ringer’s solution. The daphnia should remain in the ringer solution for 2 minutes
before being returned to the stock culture.

Testing the effects of experimental solutions
6. Place a single daphnia in the observation chamber as before.

7. Add ONE drop of your first experimental solution to the chamber. Wait one minute while the
drug takes effect. Observe the daphnia for the second minute, taking turns with your partner.
Record observed behaviors.

8. After 2 minutes of total exposure, collect heart rate data as you did for step 3 above. Record
all the data in the proper spaces on the data table.

9. When done, carefully use an eye dropper to transfer the daphnia to a miniature medicine cup
containing 5 ml Ringer’s solution. The daphnia should remain in the ringer solution for 2 minutes
before being returned to the stock culture.

10. Clean the slide and rubber washer with soap and water. Rinse thoroughly. Prepare your
observation chamber with a fresh Daphnia by repeating step 1 and 2.

6. Repeat steps 7-10 for each experimental test solutions. Record all the data in the proper
spaces on the data table.
Biology                                                              Drug Lab Techniques

                                                       Transfer this to your inquiry notes for this lab
                                                       (Don’t be lazy! It will save you time later)
Drug Effects Data                             A great general way to organize data for these experiments. Label the drug solutions you
chose to study for solutions #1, #2, and #3.

              10-second Heart        Calculations (see calculation methods below)
              Rate
Solution      3 Trials (10 second    Ave. 10 sec       Ave. 60 sec          Change in Heart         % difference in heart rate   Observed Behavior
              count every 30 sec)    Heart rate        Heart rate           rate                    (Experimental Heart rate 
                                                       (Multiply previous   (Control Heart rate –   control Heart rate) X 100%
                                     (A + B + C)  3
                                                       column X 6)          Experimental)

Control       Trial 1 (A):                                                          N/A                          N/A
              Trial 2 (B):
              Trial 3 (C):
Solution #1   Trial 1 (A):
              Trial 2 (B):
              Trial 3 (C):
Solution #2   Trial 1 (A):
              Trial 2 (B):
              Trial 3 (C):
Solution #3   Trial 1 (A):
              Trial 2 (B):
              Trial 3 (C):

GRAPHING DATA:

Create Graph that will help you compare the control group to the experimental groups. You must decide if a Bar or Line graph is more
appropriate. Also you need to decide what data is most useful to compare: Ave. 60 second heart rate or % difference (change) in heart rate.

								
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