P008 Clarity Advanced by d7qPyB

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									                            Clarity User Training




                            Clarity User Training
                                  Advanced




Code P008 Date: 20.2.2007
                                                    1
                            For advanced users


                        User settings and display options
                        Sequences
                        Multidetector systems
                        Export data
                        Report setup
                        Batch data reprocessing


Code P008 Date: 20.2.2007
                                                            2
                            For advanced users


                        Data archivation
                        Column Performance
                        Noise and Drift
                        Special calculations
                        Control modules
                        GLP a CFR21part11


Code P008 Date: 20.2.2007
                                                 3
                            User settings


    User settings are stored in
  individual users desktop *.dsk
                files.
  For this reason they are set in
 the instrument window and not
        in the main window




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                            Labels and display options




                                         Overlay
                                        activation                     Math
                                                       Moving and    operation
                                                        Scaling of       s
                                                     Chromatograms



      Text Label and Lines
     for legends and arrows
   Chromatogram/Create Label




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                            Colored peak area in chromatogram




                                                        Peaks in calibration can
                                                        have a color assigned.


                                            When these peaks are identified
                                             in chromatogram they will be
                                                 automatically colored




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                            Colored peak area in chromatogram

                        Highlight currently selected peaks




                                                    Simply select a peak in the
                                                    chromatogram result table.

                                                    The result table selection
                                                    overrides the color defined
                                                    in calibration




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                            Colored peak area in chromatogram



                                                 Use Peak Color set in
                                                 Calibration file



                Highlight peaks selected in
                Results table
                (signal color used)




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                            Table customizing options




                                                        Context menu
                                                          invoked by
                                                         right mouse
                                                         click in table



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                            Common columns in Summary table

                        Independent setting of columns
                          Common - for all compounds
                          Summary – compound specific




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                            Summary table options




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                            Graph display options


   Context menu invoked
   by right mouse click in
   Chromatogram window




                                                                 Axis properties
                                                                     setting


                                                      Graph
                                                    properties
                                                     setting

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                            Chromatogram display options


                             Setting for
                             individual
                               signals




                                                  Gradient display
                                                      options



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                            Customizing Toolbars




                                                       Reset All
                                                   restores original
                                                       settings



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                            Sequence table


             Define a serie of samples to be measured
             Essential for working with autosamplers
               For controled autosamplers the Vial number and Injection
                     volume are used from this table
             Allows modifications during a run for not yet processed
             samples
               Simply add samples by filling any field in the last empty row.
                     Other fields will be copied from previous. Several samples can
                     be added by copying from a table
             Allows for import of sample data from text files
             Allows reprocessing of last measured sample set

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                            Sequence table window

                                                    File name,       Automatic        Individual
                  Start, End vial and Injections    automatic       recalibration      Post-Run
                  number. Several vials and/or     %variables for       from         settings for
                injections defined on single row    SV, EV, IV        sequence         each row




                                                                         Tooltip with actual file
                            Use row, add new                                      name
 Row status                    rows during
  indicator                   sequence run

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                            Sequence options

                                                         Pasive sequence
                                                 Clarity only expects Start signal,
                                               analysis time is set on autosampler
                                                         Active sequence
                                                  Clarity sends a Ready signal to
                                                 autosampler and waits for Start
                                                               signal
                                                 AS control (Active sequence)
                                               Autosampler injects according to SV,
                                                             EV, IV and
                                                  Inj. Volume in sequence table



                                                     Initial value
                                                        for %n

                                                              Editation of template
                                                                method used on
                                                                  selected row


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                            Sequence control

                                  Run sequence                                  Resume sequence
                            starts complete sequence             Pause         continues from last
                                (resets the status)            sequence             injection




                                                                           Skip
                                                                           Vial


                                 Stop sequence will stop the
                                  sequence run. Repeated                   Repeat
                                  Stop will stop the current              Injection
                                           analysis


                 Status of the sequence.
               Stored at Pause, Abort, Stop
   Can be changed by Edit/Reset Status for selected rows
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                            Sequence import



                                                 Select the file to import




 Assign the respective fields to be imported
 Only Start vial (SV) and file name must be
            set (marked in bold)
 Other fields will be filled by default values


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                            Export data


             Export data to other programs to prevent typing
             errors
               Simple Copy and Paste (Ctrl C – Ctrl V)
             Export Data settings common for
              manual export from chromatogram window
              automatic export from postrun at the end of each run
              automatic export by batch reprocess of
                     chromatograms or sequence
             Export of chromatograms in common formats
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                            Export data - simplest




    Copy a picture to MS
   Office (including labels)
   via Clipboard or store it
       in *.emf format




                                               Copy a selected part of table using
                                                Copy (Ctrl C) and Paste (Ctrl V)



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                            Export data - options

                                                                      Export data:
                                                         manual - from Chromatogram window
                                                        automatic - according to Setting/Postrun
                                                             batch - from Analysis/Batch



  Export time/signal
      data pairs
   (import to other
    programs – for
  example graphics)

           Headers:
     Column headers and
      chromatogram info
          Full format:
   Filename, date and time
        before each row

                              blank field = actual chromatogram name     All exported
                                           + .txt extension              data are in
                                           Path can be used               single file

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                            Export data - example




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                             Batch processing
                             print
                                 Report style from Report Setup dialog will be used
                                 do not forget to switch off the Overlay mode
                             export
                             reprocess of chromatograms according to instrument method
                             reprocess whole sequence                               Export according
                                                                                      to Settings -
                             electronic signature                                     Export Data




                                                          File type
                                                     Chromatograms
                                                   Calibration standards
                                                      Sequence Files


                                                    Electronic signature for
                                                   series of chromatograms

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                            Multidetector systems


             Assignment of detectors to Clarity Instruments
             in the System Configuration dialog
             All signals stored in a single chromatogram
               Result table, Integration table and Acquisition
                     parameters are individual for each chromatogram
             Common calibration file
               calibration curves are individual for each signal
             Summary table for display of results from all
             signals

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                            Multi signal chromatograms



                             Connecting link marks signals from one
                             chromatogram. Click icon to set signal
                                             active




                                                                           Detector Delay
                                                                          compensates for
                  Results are displayed individually,                  interdetector volume,
                 some properties like calibration are                 inactive for first signal
                common for the whole chromatogram




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                            Overlay

                                                                                        Ctrl + click will
                                                                                     hide/show the signal



                            Ctrl + click will lock        In overlay, several
                             the tool for repe-         chromatograms can be
                                 titive use.         compared. Maximum number                Scale, move
                                                       can be set in user options             and math
                                                                                            operations are
                                                                                            active only in
                                                                          Doubleclick on       overlay
                                                                          legend will set
                                                                           signal active



                                                                     Summary table displays
                                                                         results for several
                                                                        chromatograms or
                                                                      signals simultaneously



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                            Report Setup - logo




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                            Report Setup – tiled signals




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                            Archiving and backup


            To reduce the data size compressed archive
            *.dgz files should be used
            *.dgz archives always replace all data
            (files deleted from project will be during
            archiving deleted also from the archive).
            It is advantageous to archive whole projects
            – should be considered when planning
            project structure

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                            Archive data

                             Archive and Restore for copying or moving
                                    projects
                                    Selected file types
                                    Without compressing = original format and structure
                                    compressed *.dgz archives = single file




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                            Performance parameters



                                      Separation
                                      parameters



                                                                      Advanced tab in the
                                                                     Method Setup (displays
                                                                       template method
                                Values used for Capacity ratio and
Performance tab                       Efficiency calculation
   (hidden in
 default layout)



                                                                          Calculation type



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                            Noise monitoring




                   Actual noise monitoring in the
                      Data Acquisition window


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                             Noise and Drift




                                                         Drift and Noise can
                                                         be used for in User
                                                         column calculations




                            Drift and Noise displayed
                             for selected intervals in
                               Result table header



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                            Calculations

                             ISTD calculations
                             Normalized % calculations
                             Groups
                                  calibration of peak group like single compound
                                  example - isomers (xylen)
                             Scale factor
                                  recalculation of result to other units
                             Uncalibrated response
                                  quantification of unidentified peaks using Uncalibrated
                                   response factor
                             Injection volume
                                  Injected volume correction
                                  for manual injection in GC
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                            ISTD calculations

                                                        ISTD calculations are performed:
                                                        a) Amount for ISTD is set in both
                                                           calibration and sample
                                                        b) both are zero




       ISTD compound is
           calibrated


     Only one ISTD
 compound is allowed                 ISTD peak can
  in calibration. If the            be hidden in the
   conditions are not                 Result table
 met, no results will be                               ISTD amount in
       calculated                                      sample is given

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                            Normalized % calculations


                                 Amount % gives the
                                  normalized % if all     If Sample amount is non-
      Total line is                                     zero, it will replace the sum
    calculated as a              integrated peaks are
                                calibrated and Sample    in the Amount column and
  sum of all groups                                     serve as a base for Amount
   + sum of peaks                   amount is zero
                                                                % calculation
   not belonging to
      any group.
      Groups are
   calibrated, thus
  peaks should not
  be both calibrated
  and in a group to
      get correct
  Amount% values




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                            Groups

                                               1st step
                                     Adding peaks to a group
                                      in the integration table




                 2nd step
           Setting a Group in
            Calibration table.
          The compound name
         and amount is set as for
             ordinary peaks

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                            Scale Factor and Uncal.Response

                                                         Uncal. Response:
                                                     Used for quantification of
                                                    unidentified compounds. For
                                                    universal response detectors
        Scale factor and Units after scaling:             (ELSD, FID, RI)
       Multiplies values in Amount column and
        changes the units in column header.
      Method specific parameter, multiplies also
         the entered Sample Amount value




                           Dilution:
            Multiplies values in Amount column.
          Sample specific parameter (entered from
           Sequence or Single Analysis window).

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                             Injection Volume

                             Default Injection Volume must be set first in calibration
                              and Injection Volume set in standards before calibrating
                             Supposes linear detector response
                                                                                    Setting Injection
                             Can lead to incorrect results, if used improperly        Volume for
                                                                                      sample and
                                                                                        standard




     Setting
     Default
    Injection
   Volume in
   calibration




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                            Calibration




                                                            Command
                                                        Calibration/Show
                                                        History displays
                                                         data used for
                                                         selected level
                                Deviation of
                                 point from
                                 calibration
                                curve in %



                                     Residual sum of
                                    deviation squares

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                            Retention indexes, LOD, LOQ

                              Calibration
                                   Retention (Kovats) indexes
   Columns for Retention           LOD and LOQ limits
    (Kovats) indexes are
      hidden in default
          settings.
   Use setup columns to
       disdplay them




                                                                  Amounts lower then
                                                                 specified LOD or LOQ
                                                                  limits are marked in
                                                                    Peak type column




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                            Control Modules


             For selected instruments the direct control is
             available. The advantages are
             The instrument parameters are stored in a
             method
             It is not necessary to enter the parameters
             twice, especially useful for autosamplers
             The parameters are recorded in the
             chromatogram

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                            Control modules (GC)




     Control modules enable full
    control of connected devices
     like gradient programe and
       other settings. The used
     settings are stored within a
            chromatogram.

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                            Control modules (LC, AS)




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                            Control Modules

                     Option to Send/Confirm/Do not send method to
                     instruments after method change




                                                   Confirmation dialog


                                                    Send Method options



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                            Control modules overview




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                            Additional control

                     Other devices can be controlled by A/D board digital outputs:
                           gradient or detector program start
                           detector autozero
                           valve switching
                           fraction collector control
                     Synchronization in Active Sequence
                           Dig. output set in System Configuration, not in the Event table




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                            Event table

            Set events in the Event Table tab in the Method Setup
            Named events
            Digital event input type
            Output event can trigger run/sequence commands:
              Stop
              Abort
              Skip vial
              Shutdown
              Repeat injection



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                            GLP a CFR21 part 11


             Chromatogram history
                         All saved versions are stored in the chromatogram
                         Versions can be opened (including calibration)
             Audit trails
                         Station, Chromatogram, Sequence, Calibration
             User access
                         Can be restricted by User Accounts settings




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                            GLP a CFR21 part 11


            Electronic signatures
                        based on User Accounts
                        based on Certificates
            Validation
                        Installation qualification - standard feature
                        Performance qualification - by optional Validation kit
                        Operation qualification - SST module for statistical
                            evaluation and pre-set limits control




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