Docstoc

AS 2411-1993 Venous blood - Determination of lead content - Flame atomic absorption spectrometric method

Document Sample
AS 2411-1993 Venous blood - Determination of lead content - Flame atomic absorption spectrometric method Powered By Docstoc
					                                                                                                             AS 2411—1993




                                                                                           Australian Standard®
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                           Venous blood—Determination of
                                                                                           lead content—Flame atomic
                                                                                           absorption spectrometric method
                                                                                           This Australian Standard was prepared by Committee CH/6, Analysis of Body Fluids
                                                                                           for Metals Content. It was approved on behalf of the Council of Standards Australia
                                                                                           on 19 April 1993 and published on 14 June 1993.




                                                                                           The following interests are represented on Committee CH/6:

                                                                                                 Australian Association of Clinical Biochemists
                                                                                                 Australian Chamber of Commerce and Industry
                                                                                                 Australian Institute of Medical Scientists
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                                 Australian Institute of Mining and Metalurgy
                                                                                                 Australian Mining Industry Council
                                                                                                 Department of Health, New South Wales
                                                                                                 Institute of Medical and Veterinary Science
                                                                                                 Royal College of Pathologists of Australia
                                                                                                 Royal North Shore Hospital
                                                                                                 Royal Prince Alfred Hospital
                                                                                                 Womens and Childrens Hospital, Adelaide




                                                                                           Review of Australian Standards. To keep abreast of progress in industry, Australian Standards are subject
                                                                                           to periodic review and are kept up to date by the issue of amendments or new editi ons as necessary. It is
                                                                                           important therefore that Standards users ensure that they are in possession of the latest edit ion, and any
                                                                                           amendments thereto.
                                                                                           Full detail s of all Australian Standards and related publi cati ons wil l be found in the Standards Australi a
                                                                                           Catalogue of Publications; this information is supplemented each month by the magazine ‘The Australi an
                                                                                           Standard’, which subscribing members receive, and which gives details of new publications, new editi ons
                                                                                           and amendments, and of withdrawn Standards.
                                                                                           Suggesti ons for improvements to Australian Standards, addressed to the head offi ce of Standards Australi a,
                                                                                           are welcomed. Noti fi cati on of any inaccuracy or ambiguity found in an Australian Standard should be made
                                                                                           without delay in order that the matter may be investigated and appropriate action taken.




                                                                                                      This Standard was issued in draft form for comment as DR 88090.
                                                                                                                                AS 2411—1993




                                                                                           Australian Standard®
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                           Venous blood—Determination of
                                                                                           lead content—Flame atomic
                                                                                           absorption spectrometric method




                                                                                            First publi shed as AS 2411—1980.
                                                                                            Second editi on 1993.




                                                                                           PUBLISHED BY STANDARDS AUSTRALIA
                                                                                           (STANDARDS ASSOCIATION OF AUSTRALIA)
                                                                                           1 THE CRESCENT, HOMEBUSH, NSW 2140
                                                                                                                                    ISBN 0 7262 8237 5
                                                                                           AS 2411—1993                                                         2


                                                                                                                                                          PREFACE

                                                                                                 This Standard was prepared by the Standards Australia Committee on Analysis of Body Fluids for
                                                                                                 Metals Content, under the direction of the Multitechnics Standards Policy Board, to supersede
                                                                                                 AS 2411—1980, Determination of lead in venous blood (flame atomic absorption spectrometric
                                                                                                 method).
                                                                                                 The Committee organized an inter-laboratory test program to obtain information on the repeatability
                                                                                                 and reproducibility of the method. The following laboratories participated in the test program to
                                                                                                 provide the data given in Tables 2, 3, A2 and A3:
                                                                                                     Department of Health, New South Wales
                                                                                                     Hampson and Associates
                                                                                                     Institute of Medical and Veterinary Science
                                                                                                     Mount Isa Mines
                                                                                                     Pasminco Metals - BHAS
                                                                                                     Pasminco Metals - EZ
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                                     Pasminco Metals - Sulphide
                                                                                                     Womens and Childrens Hospital, Adelaide


                                                                                                                                                        CONTENTS
                                                                                                                                                                                                                                                       Page
                                                                                                   1   SCOPE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .   ..   .   .   .   .. ..   .   .   ..    ..    .   ...    .   .   . 3
                                                                                                   2   REFERENCED DOCUMENTS . . . . . . . . . . . . . . . . . . .                      ..   .   .   .   ....    .   .   ..    ..    .   ...    .   .   . 3
                                                                                                   3   PRINCIPLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .     ..   .   .   .   ....    .   .   ..    ..    .   . ..   .   .   . 3
                                                                                                   4   REAGENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .        ..   .   .   .   .. ..   .   .   ..    ..    .   . ..   .   .   . 3
                                                                                                   5   APPARATUS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         ..   .   .   .   ... .   .   .   . .   . .   .   .. .   .   .   . 4
                                                                                                   6   SAMPLING, SAMPLE PREPARATION AND STORAGE                                        ..   .   .   .   . ...   .   .   ..    ..    .   ...    .   .   . 5
                                                                                                   7   PROCEDURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         ..   .   .   .   ... .   .   .   . .   . .   .   .. .   .   .   . 5
                                                                                                   8   CALCULATION OF RESULTS . . . . . . . . . . . . . . . . . .                      ..   .   .   .   ....    .   .   ..    ..    .   ...    .   .   . 7
                                                                                                   9   PRECISION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .       ..   .   .   .   .. ..   .   .   ..    ..    .   . ..   .   .   . 7
                                                                                                  10   TEST REPORT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .         ..   .   .   .   ....    .   .   ..    ..    .   ...    .   .   . 7

                                                                                                 APPENDICES
                                                                                                  A PROCEDURE FOR SAMPLES WITH LEAD CONTENT LESS THAN 1.5 µmol/L . . . . 9
                                                                                                  B RECOMMENDED PRECAUTIONS FOR PERSONS PERFORMING LABORATORY
                                                                                                    TESTS ON CLINICAL SPECIMENS OR OTHER POTENTIALLY INFECTIOUS
                                                                                                    MATERIAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11




                                                                                                                                          © Copyri ght STANDARDS AUSTRALIA
                                                                                             Users of Standards are reminded that copyri ght subsists in all Standards Australi a publications and soft ware. Except where the
                                                                                             Copyri ght Act all ows and except where provided for below no publications or software produced by Standards Austr alia may be
                                                                                             reproduced, stored in a retri eval system in any form or transmitt ed by any means without pri or permission in wri ti ng fr om
                                                                                             Standards Australi a. Permission may be conditi onal on an appropriate royalt y payment. Requests for permission and information on
                                                                                             commercial soft ware royalti es should be dir ected to the head off ice of Standards Australi a.
                                                                                                 Standards Austr alia will permit up to 10 percent of the technical content pages of a Standard to be copied for use exclusively
                                                                                                 in-house by purchasers of the Standard wit hout payment of a royalty or advice to Standards Austr alia.
                                                                                                 Standards Austr alia wil l also permit the inclusion of it s copyright material in computer software programs for no royalt y
                                                                                                 payment provided such programs are used exclusively in-house by the creators of the programs.
                                                                                             Care should be taken to ensure that material used is fr om the current editi on of the Standard and that it is updated whenever the
                                                                                             Standard is amended or revised. The number and date of the Standard should therefore be clearly identif ied.
                                                                                             The use of material in pri nt form or in computer soft ware programs to be used commercially, with or without payment, or in
                                                                                             commercial contracts is subject to the payment of a royalty. This policy may be vari ed by Standards Austr alia at any ti me.
                                                                                                                                           3                                          AS 2411—1993


                                                                                                                           STANDARDS AUSTRALIA


                                                                                                                              Australian Standard
                                                                                                            Venous blood—Determination of lead content—
                                                                                                            Flame atomic absorption spectrometric method



                                                                                           1 SCOPE This Standard sets out a flame atomic absorption spectrometric method for the
                                                                                           determination of lead in venous blood. The method is suitable for whole blood with lead
                                                                                           content in the range 1.0 µmol/L to 6.0 µmol/L. Precautions shall be taken to prevent
                                                                                           coagulation or clots from forming in the sample. The sample shall be preserved, prior to
                                                                                           analysis, under the recommended conditions (see Clause 6). Where it is anticipated that the
                                                                                           lead content will be less than 1.5 µmol/L, the procedure outlined in Appendix A is
                                                                                           recommended.
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                           The procedure for sampling blood for the determination of lead is given in AS 2636.

                                                                                           2 REFERENCED DOCUMENTS The following documents are referred to in this
                                                                                           Standard:

                                                                                           AS
                                                                                           2134     Recommended practice for chemical analysis by atomic absorption spectrometry
                                                                                           2134.1   Part 1: Flame atomic absorption spectrometry

                                                                                           2162     Code of practice for the use of volumetric glassware

                                                                                           2164     One-mark volumetric flasks

                                                                                           2166     One-mark pipettes

                                                                                           2252     Biological safety cabinets

                                                                                           2636     Sampling of venous and capillary blood for the determination of lead or cadmium
                                                                                                    concentration

                                                                                           2850     Chemical analysis—Interlaboratory test programs—For determining precision of
                                                                                                    analytical method(s)—Guide to the planning and conduct

                                                                                           3 PRINCIPLE The lead is complexed with ammonium 1-pyrrolidinecarbodithioate and
                                                                                           extracted into a 1-butyl acetate solution. Lead is determined in the organic phase by atomic
                                                                                           absorption spectrometry.

                                                                                           4 REAGENTS

                                                                                           4.1 General requirements All reagents shall be of analytical grade and distilled or
                                                                                           deionized water shall be used throughout. The reagents and the water shall be shown to
                                                                                           contain a lead concentration of less than 0.1 µmol/L.
                                                                                               Warning: The reagents and solutions used in this method may constitute a health hazard and due
                                                                                               care should be taken in their handling.




                                                                                                                                     COPYRIGHT
                                                                                                   This is a free preview. Purchase the entire publication at the link below:




                                                                                                             AS 2411-1993, Venous blood - Determination of
                                                                                                             lead content - Flame atomic absorption
                                                                                                             spectrometric method
This is a free 5 page sample. Access the full version at http://infostore.saiglobal.com.




                                                                                              Looking for additional Standards? Visit SAI Global Infostore
                                                                                              Subscribe to our Free Newsletters about Australian Standards® in Legislation; ISO, IEC, BSI and more
                                                                                              Do you need to Manage Standards Collections Online?
                                                                                              Learn about LexConnect, All Jurisdictions, Standards referenced in Australian legislation
                                                                                              Do you want to know when a Standard has changed?
                                                                                              Want to become an SAI Global Standards Sales Affiliate?

                                                                                           Learn about other SAI Global Services:

                                                                                              LOGICOM Military Parts and Supplier Database
                                                                                              Metals Infobase Database of Metal Grades, Standards and Manufacturers
                                                                                              Materials Infobase Database of Materials, Standards and Suppliers
                                                                                              Database of European Law, CELEX and Court Decisions




                                                                                                          Need to speak with a Customer Service Representative - Contact Us

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:8
posted:7/5/2012
language:
pages:6
SAIGlobalAPAC SAIGlobalAPAC http://
About