Drosophila Embryo Cuticle Preps

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					 Drosophila Embryo Cuticle Preps
1.  Dechorionate embryos (50% bleach for 2 minutes)
2.  Add embryos to small snap-cap tube with 2 mls heptane
    and 2 mls methanol
3. Shake hard for 1 minute (embryos should fall to the
    bottom)
4. Remove heptane (top layer)
5. Add 1-2 mls methanol
6. Shake for 30 seconds
7. Remove most methanol and transfer embryos to a glass
    filter disc (filamentous side down)
8. Wash with the remaining methanol on the aspirator
9. Wash with water
10. Remove embryos one at a time from the filter and place
    in a small patch of Hoyer’s solution on a glass slide
11. Place a coverslip over the embryos (add more Hoyer’s as
    needed)
12. Place at 70 overnight

				
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Lingjuan Ma Lingjuan Ma
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