Comparison of gallic acid content in different Triphala churna formulations by HPTLC
Prathyusha M, Preethi Samantha, Muruganantham N, Nanjan MJ.
JSS College of Pharmacy (Off Campus, JSS University, Mysore), Rocklands, Ooty, India.
The present study deals with the comparison of gallic acid content in Triphala churna.
Triphala is one of the well known powdered preparations in Ayurveda since ancient times. It is
mentioned throughout ancient literature of Ayurveda as a tonic alterative and gentle aperient and
also highly prized for its ability to regulate the process of digestion and elimination. This
formulation consists of Terminalia chebula, Terminalia belerica and Embellica officinalis, in
equal proportions. Owing to its importance in the treatment of various ailments, we felt the need
to evaluate the quality of commercial Triphala churnas. Hence a HPTLC method was developed
and validated for estimation of gallic acid (active constituent) in the formulation. Toluene: ethyl
acetate: formic acid (4:5:1, v/v/v) was used as the mobile phase and the developed plate was
detected at 295 nm. The method was also validated for its specificity, sensitivity, linearity,
precision and accuracy. Quantification of gallic acid in three different Triphala churnas namely
churna A, churna B and churna C was performed by the developed method.
Results of our study revealed that Churna B (3.541 ± 0.0155% w/w) has high amount of
gallic acid content compared to churna A (0.989 ± 0.016% w/w) and churna C (1.706 ± 0.004%
w/w). It is clearly evident from our study that each Triphala churna has different amount of gallic
acid content. This shows that the herbal formulations available in the market have not been
standardized as per the marker concept. It is for the regulatory authority to take necessary steps
to implement the standardization rules to the herbal industry.