KERATIN 5 RABBIT ANTIBODY
PRESENTATION: 7.0ml prediluted antibody which is ready-to-use for staining of
formalin-fixed, paraffin-embedded tissues.
IMMUNOGEN: Synthetic peptide corresponding to C-terminal of human keratin 5.
SPECIES REACTIVITY: Human. Others-not known.
DESCRIPTION: Twenty human keratins are divided into acidic (pI <5.7) and basic (pI
>6.0) subfamilies. Members of the acidic and basic subfamilies are
found together in pairs. The composition of keratin pairs varies with
the epithelial cell type, stage of differentiation, cellular growth
environment, and disease state. Many studies have shown the
usefulness of keratins as markers in cancer research and tumor
identification. Point mutations in cytokeratin5 gene can cause various
types of epidermolysis bullosa simplex. It is expressed in most
epithelial and biphasic mesotheliomas.
APPLICATION AND SUGGESTED DILUTIONS:
• Immunohistochemistry (Formalin/paraffin)
- Working dilution: Ready-to-use for 30 min at RT
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM
citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
The optimal dilution for a specific application should be determined by
MOLECULAR WEIGHT: 58kDa
POSITIVE CONTROL: Mesothelioma
CELLULAR LOCALIZATION: Cytoplasmic
STORAGE & STABILITY: Ab with sodium azide is stable for 24 months when stored at 2-80C.
LEGAL CONSIDERATION: This product is recommended FOR RESEARCH USE ONLY.
Human mesothelioma stained with
Anti-keratin 5, cat# E13821
46755 Fremont Blvd. . Fremont, CA 94538
. tel 510.979.9460
. fax 510.979.9469