Generic Biohazard Animal Biosafety SOP

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Generic Biohazard Animal Biosafety SOP Powered By Docstoc
					           Environmental Health and Safety
           Career and Protective Services

           HANDLING ANIMALS EXPOSED TO INFECTIOUS AGENTS: STANDARD OPERATING PROCEDURES

DESCRIPTION:
In the course of research, laboratory animals may be exposed to infectious agents, including viral vectors for
gene transfer. This SOP addresses procedures for performing animal caretaking safely after the animals have
been infected.

Animals have the potential to shed virus in their excreta, particularly during the first 48 hours after infection.
The precautions outlined below are designed to protect employees in these situations.


These procedures must be followed for the first week after infection AND until the contaminated bedding is
changed. All bedding used for the first week after infection will be considered contaminated. The procedures
may need to be modified depending on the type of animal, number of infections, or nature of infectious agent.


NOTIFICATION and SIGNAGE:
Laboratory staff must make arrangements for housing these animals and for disposing of the contaminated
bedding by contacting the appropriate animal caretaker supervisor before dosing begins.

When animals are exposed to an infectious agent, laboratory workers must label the cage of that animal with a
cage card that bears the name of the agent and the date of exposure. In addition, the attached sign must be
completed and attached to the front of the door where the animals will be housed.


PERSONAL PROTECTIVE EQUIPMENT:
Employees must wear appropriate personal protective equipment for handling animals, cages, and bedding:
   1.   Lab coat/gown
   2.   Latex/nitrile gloves
   3.   Shoe covers
   4.   Safety glasses (if animals handled outside of a biosafety cabinet or ventilated cage-changing station)

ENGINEERING CONTROLS:
 Animals will be housed only in micro-isolation cages.
 Cages will be changed by animal resources staff in a ventilated cage-changing station or a biological safety
  cabinet

WORK PRACTICES:
 Employees will wash hands before donning and after removing gloves.
 Gloves will be changed at least every 2 hours and when they become torn or obviously contaminated.
 Contaminated bedding shall be collected inside of a biosafety cabinet or ventilated cage-changing station.
  Bedding must be collected into labeled biohazardous waste buckets lined with red bags. Please call EH&S
  for immediate pickup of contaminated bedding (HSC: x22225, UPC: x07215).
 Decontamination of the ventilated cage-changing station or biological safety cabinet will consist of surface
  cleaning with a 5% bleach solution or other appropriate disinfectant. Cleaning will proceed from least to
  most contaminated areas.
          Environmental Health and Safety
          Career and Protective Services

          HANDLING ANIMALS EXPOSED TO INFECTIOUS AGENTS: STANDARD OPERATING PROCEDURES

ADDITIONAL INFORMATION
If you have any questions, please contact the Environmental Health and Safety Office at (323)442-2200 or send
an email to IBC@caps.usc.edu
                                        University of Southern California
                                        Laboratory Safety (323) 442-2200

                              ANIMAL HANDLER PRECAUTIONS
                               Building:                Room Number:




Certain animals in this room are part of an approved experiment involving biohazardous materials. The requirements
below apply to this experiment.

Principal Investigator:                        Department:                             Phone:

Emergency Contact Person:                      Phone:                                  IACUC Protocol:

Identification of Hazard(s):
Required PPE
   1. Lab coat/gown
   2. Latex/nitrile gloves
   3. Shoe covers
   4. Safety glasses (if animals handled outside of a biosafety cabinet or ventilated cage-changing station)


Special Instructions
   1. Prior to injection of agent, arrangements for housing and bedding disposal must be made with an animal
       caretaker supervisor.
   2. All animals must be housed in micro-isolation cages.
   3. Individual cages must be labeled with biohazard cage card upon injection of agent. The label must
       indicate the name of the agent and the date of injection.
   4. All animal handling must occur inside a certified biological safety cabinet or ventilated cage-changing
       station.
   5. Contaminated bedding shall be collected inside a biosafety cabinet or ventilated cage-changing station.
       Bedding must be collected into labeled biohazardous waste buckets lined with red bags. Please call
       EH&S for immediate pickup of contaminated bedding (HSC: x22225, UPC: x07215)
   6. Biosafety cabinet or ventilated cage changing station must be decontaminated with 5% bleach solution
       or other appropriate disinfectant after use or cage change.
   7. Remove gloves and wash hands with soap and water upon leaving room.

				
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