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					DNA Extraction
DNA Extraction from Plant Material

                 DNA is extracted onto the
                 paper matrix.
                 The FTA card is a chemically
                 treated paper matrix for the
                 safe collection, transport and
                 storage of DNA.
                 Before PCR, inhibitors of the
                 reaction have to be removed
                 from the card.
  DNA Extraction from Plant Material

When a plant sample is pressed onto the FTA
matrix, moisture in the sample activates
chemicals in the FTA card and these chemicals
lyse the cells, denature enzymes, inactivate
pathogens and immobilise the DNA.
Once completely dry, such plant extracts can be
stored for several years at room temperature.
This method is not suitable for fibrous leaves
such as cabbage or pine, which are difficult to
crush.
DNA Extraction from Plant Material




                           Place a
                           backing board
                           between the
                           back cover of
                           the card and
                           the absorbent
                           layer.
DNA Extraction from Plant Material


                           Place a piece of
                           fresh plant
                           material onto
                           one box on the
                           FTA card.
                           Ensure it does
                           not extend
                           outwith the box.
                           Close cover.
DNA Extraction from Plant Material


          Using a pestle, squash the leaf onto the
          card until moisture has soaked through
          to the back of the absorbent layer.
          Discard squashed plant material.

          Try to keep the sample within the box.
   DNA Extraction from Plant Material

                        Repeat extraction for a
                        second plant material
                        in a different box.




Try to ensure each
sample stays within
its own box.
      DNA Extraction from Plant Material



Make sure that
moisture from
the leaf has
soaked through
to the back of
the paper.
      DNA Extraction from Plant Material


                            Repeat extraction for
                            the third and fourth
                            plant materials in the
                            remaining boxes.




Try to ensure there is no
‘escape’ of extracted
materials to other boxes.
     DNA Extraction from Plant Material




Label
your
samples.
DNA Extraction from Plant Material




                            Leave cards
                            open to dry.

				
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posted:6/20/2012
language:English
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