Be able to calculate RBC indices given appropriate data by HC12061302359


									Hematology Laboratory: Hemoglobin, Hematocrit, RBC Indices

                         CLS 312


  At the completion of this laboratory the student should be able to:

1. Perform 10 micro hematocrits.

2. Determine the approximate hemoglobin value based on the hematocrit.

3. Be able to calculate RBC indices given appropriate data.

The hematocrit, or packed RBC volume, is the ratio of the volume of RBC's to the volume of whole blood.
 Hematocrit is usually expressed as a percentage. The hematocrit should be approximately 3 times the

Microhematocrit Determination

I. Materials needed:

   capillary tubes
   hematocrit centrifuge
   gauze squares

II. Procedure:

   A.        Fill capillary tubes 3/4 full of blood.

   B.        Wipe off excess blood with gauze square.

   C.        Place finger on end of tube and carefully press other end into clay to plug.

   D.        Spin in hematocrit centrifuge for 5 minutes at 11,000 rpms.

   E.        Determine volume of packed red cells, expressed as a percentage of the total volume. Do not
             include the buffy coat in the reading.
III. Interpretation of Results

    Normal values:               women      37-47%
                                 men        40-54%

    The hematocrit is decreased in anemia and increased in polycythemia and dehydration.

IV. Sources of Error

    A. inadequate mixing of blood

    B. excess anticoagulant causes RBC's to shrink, decreasing the hematocrit

    C. inadequate centrifugation

    D. including buffy coat in reading

6/12/2012 7:51:00 PM

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