UCTF day 2011 Coffee breeding Dr Pascal Musoli COREC by 1q91CylD

VIEWS: 19 PAGES: 21

									 SELECTION AND PROPAGATION OF 7 ELITE
CWD RESISTANT ROBUSTA COFFEE VARIETIES



                   P.C. Musoli,

   Coffee Research Centre, P.O. Box 185, Mukono
                  RESEARCH TEAM


Scientists        Technicians        Collaborating
1. P.C. Musoli    1. S. Olal           Institutes
2. J. G. Hakiza   2. A. Nabaggala
3. J. Mwesigwa    3. C. Kabole
                                     1. UCDA
4. P. Wetala      4. S. Nakendo
5. P. Aluka       5. J. Pande        2. CABI
6. P. Kucel       6. J. Ekwaru       3. CIRAD
7. I. Sebugenyi   7. N. Edongot
8. A. Nalukenge   8. G. Ebong
9. R. Matovu      9. D. Musiime
10. D. Kyetere    10. S. Okiror
11. J. Ogwang     11. L. Chepkurui
12. A. Kangire
Coffee Wilt Disease (CWD) has been the major constraints of
Robusta coffee production since 1993
COREC started a breeding program in 1997 to develop
Robusta coffee varieties:

• Resistant to CWD
• Resistant to other major coffee diseases
   – Leaf rust
   – Red blister disease
• High yielding
• Have acceptable market qualities
   – Good cup quality
   – Good physical bean qualities
               1) Evaluated Robusta coffee germplasm for resistance
                  against CWD through assessment in the field under
                  natural infection (20 clones studied for 6 years)
               2) Selected 3 clones for multi-location testing en route to
                  release for cultivation
              100.0                                                                                                  100.0
                                                                                         Highly
               90.0                                                                                                   90.0
                                                                                         susceptible
               80.0                                                                                                   80.0




                                                                                                       % mortality
               70.0                                                                                                   70.0
% mortality




                                                                                         Moderately
               60.0                                                                      susceptible
                                                                                                                      60.0
               50.0                                                                                                   50.0
               40.0                                                                                                   40.0
               30.0                                                                      Moderately                   30.0
               20.0                                                                      resistant                    20.0
               10.0                                                                                                   10.0
                0.0                                                                      Resistant                     0.0




                                                                                                                               1-




                                                                                                                                                                                         66
                                                                                                                               7
                                                                                                                                             13


                                                                                                                                                      25
                                                                                                                                                           31
                                                                                                                                                                37
                                                                                                                                                                     43
                                                                                                                                                                          49
                                                                                                                                                                               55
                                                                                                                                                                                    61
                       1-

                       7 il 0
                       13



                                          25

                                               31
                                                     37

                                                            43

                                                                 49

                                                                       55

                                                                              61

                                                                                   66




                                                                                                                                  Ap




                                                                                                                                                                                           -M
                          Ap




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                                                                                                                                    ril




                                                                                                                                                                                             ar
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                                                                                       ar




                                                                                                                                        01




                                                                                                                                                                                                ch
                                                                                          ch




                                          Time in months
                              1




                                                                                                                                                     Time in months




                                                                                                                                                                                                06
                                                                                           06




                      E/3/2       223/32            1S/2              B/1/1           J/1/1                                  H/4/1            C/1/7         Q/1/1          257/53           C/6/1
                      G/3/7       Q/6/1             P/3/6             L/2/7           B/6/2                                  P/5/1            1S/3          Q/3/4          R/1/4            B/2/1




              Quantitative response to CWD by the Robusta coffee clones
        Mortality after 66 months

100.0


 90.0


 80.0


 70.0


 60.0


 50.0


 40.0


 30.0


 20.0


 10.0


  0.0
2) Evaluated Robusta coffee germplasm for resistance against
CWD through screen house assessment after artificial
inoculation of tens of thousands seedlings using root dip
method




            Susceptible plants        Survivors
• Planted all CWD resistant individuals (1500) identified
  from artificial inoculations into mother gardens at Kituza
  (at different times)
Cloned and planted CWD resistant individuals in rows of 6
                 trees for assessment
Evaluated the CWD resistant clones in on-station
field trials at Kituza:
      •Yield
      •Quality
      •Resistance to other diseases




                                             Leaf rust




                                           Red blister
25 with high potential were planted in multi-location trials at
         Ibanda, Nakanyonyi, Kamuli and Mityana
  7 best lines were selected and released by the national
        variety release committee as new varieties

                                                 100 bean   %          CWD                    Resistance   Yield
                                                 weight     Retained   resistan   Resistanc   to Red       (kg/cc/
Source       Variety      Body      Flavour      (gm)       by 16/64   ce         e to rust   blister      ha)

Identified   E.u 20       Fair      Fair-          17.6        83.1       1          1               1     1125*
through      E. U 14      Fair      Fair+          19.1        87.3       1          1.5             1     1825*
artificial
inoculatio   2/22/12      Fair+     Fair+          19.2        95.8       1          1               1     2300*
n            J1/14/21/1   Fair      Fair+          21.1        95.4       1          1.6             1     2100*
Identifie    J/1/1         Fair+    Fair++                     92.4       1          1.5             1     2940+
d thru
             Q/3/4         Fair+    Fair+                      87.3       2          1               1     2490+
infected
fields       R/1/4         Fair+    Fair+          21.4        79.7       2          1               1     2210+
             1s/3         Fair+     Fair+          20.5        80         3          1               1     2130+
Checks
             1s/6                                  23.0        80         5          1               1     2240+


 Key
 1= no disease ; 2 = 1-25 diseased; 3 = 26-50% diseased; 4 = 51-75% diseased; 5 = 76-100% diseased
 * = Yield for only 1 year and was affected by thefts
 •+ = average yield for 6 years
                         Variety Kituza R7 (J1/14/21/1
Released varieties
R/1/4 = Kituza R1
Q/3/4 = Kituza R2
J/1/1 = Kituza R3
E. U 14 = Kituza R4
E.u 20 = Kituza R5
2/22/12 = Kituza R6
J1/14/21/1 = Kituza R7
      Propagation of CWD resistant varieties

• The varieties are being maintained in mother gardens and
  field plots at Kituza
• The varieties have to be propagated exclusively by
  vegetative methods (rooted cuttings and tissue culture) due
  to segregation of the CWD resistance
• COREC provide rooted cuttings or tissue culture plant lets
  of these varieties to private nursery operators and
  government institutions for further multiplication and
  distribution to farmers
           Beneficiary nursery operators (so far)
     OPERATOR                      DISTRICT   SUB COUNTY   PARISH       Plants received



     Mukono ZARDI                  Mukono     Mukono TC    Ntawo                     240
 1
     Mpimbe L                      Masaka     Kibinge      Kiryasaaka                150
 2
     Mukiibi B                     Masaka     Kkingo       Kiterdde                  152
 3
     Kalisizo Crop Nursery(KAVA)   Rakai      Kalisizo     Kalisizo                  194
 4
     Semakula Richard              Luwero     Wobulenzi    Wobulenzi                 182
 5
     NUCAFE                        Mpigi                                                  25
 6
     DAO                           Buikwe                                            200
 7
     Mr. Lubega David              Rakai      Kabira       Bwamijja                  145
 8
     Kabunanga Investments         Mityana    Busimbi      Katakala                  200
 9
     Nakanwagi                     Mubende    Kaweri       Kaweri                    200
10
     R. Buwamatsiko                Mubende    Kigando      Kigando                   240
11
     Job Coffee                    Nakaseke                                          313
12
     Senoga Kennedy                Mityana                                           145
13
     Dr. Tumwine J                 Kamwenge                                          149
14
     Mountain view farm            Bushenyi                                               25
15
                  Work Still In Progress

• Assessment of varieties in the existing multi-location
  trials (Mityana, Nakanyonyi, Kamuli and Mukono Ibanda)
  for resistance against leaf rust, and red blister; quality
  and yield is till still in progress

• Over 1,000 lines have still not yet been evaluated in the
  on-station trial at Kituza
                                                                                                                                                                                   varieties

                                                                                                       1               2                 3                4                 5                  6            7             8                 9                10            11



                             Assessment scale   Cut of level                                2/22/3          E.U/11          227/54/2          14/50/1          202/63/2          J24/13/20/1 J72.01/10/1 J24/13/22/1 3/71/1                      J24/13/59/9 UFCT3

General Appearance           1-4                = or > 3                                              3.0             3.1               3.8              3.1               3.4             3.0             3.0           3.3               3.3               3.0           3.1

Yield Potential              1-4                = or > 3                                              2.9             3.0               3.8              3.0               3.1             3.0             2.9           2.6               3.0               2.9           3.0

Cherry Size                  1-4                = or > 2                                              2.6             2.9               2.8              2.3               2.9             2.3             2.3           2.1               2.7               2.6           2.7

Suckering Ability            1-4                = or > 2                                              1.8             2.3               2.7              2.2               2.4             2.1             2.4           2.6               2.0               2.7           2.1

Red Blister                  1-4                = or < 2                                              1.9             2.1               2.2              1.4               1.6             1.9             1.1           1.4               1.1               1.1           1.4

Coffee Leaf Rust             1-4                = or < 2                                              1.5             1.5               1.5              1.5               1.3             1.1             1.3           1.1               1.1               1.9           1.9

% moisture                   %                  11-13%                                               14.3            13.2              12.0             13.5              15.8           12.6             14.7          13.1              14.2              13.5          14.4



% wt retained by screen 18   %                                                                       50.9            18.2              30.7             21.1              12.8             8.9            10.3          20.0              27.5              12.8           3.4



% wt retained by screen 15   %                  80%                                                  97.9            92.3              91.4             92.9              94.9           87.6             81.0          83.9              92.5              90.3          79.4



density                                         Not considered                                        4.2             4.9               4.6              4.8               4.7             4.9            10.0           5.4               3.9               4.6           4.9



Fragrance                                       Not considered                                        3.0             2.5               3.0              1.5               3.0             1.5             3.0           2.0               2.0               2.0           2.0

Body                         1-10               = or > 5                                              6.5             6.5               7.5              6.0               6.0             6.5             7.0           7.5               5.5               5.5           5.0

Flavour                      1-10               = or > 5                                              6.0             6.5               6.5              6.0               6.8             7.5             7.0           7.0               6.0               6.5           5.0



Acidity                      1-10               Not considered                                        0.0             0.0               0.0              1.0               3.0             1.0             1.0           0.0               1.0               1.0           1.0



Roast                                           Not considered                              Dull            Dull            Dull              Dull             Dull              Dull              Dull          Dull          Ordinary          Ordinary          Dull



Taste (AT)                                      Not considered                                         2               2                 3               1.5                3                  2           2.5            1                 1                2.5            1




Visual apperance

Yield potential
                             Poor (1)

                             Low
                                                Fair (2)

                                                Medium
                                                                 Good (3)

                                                                 Good
                                                                            Very good (4)

                                                                            High
                                                                                                            11 newly selected lines are
Cherry size                  Small              medium           Big        Very big
                                                                                                            being propagated for planting in
                                                                                                            multi-location trials
Incidence of Red blister     None               minor            medium     high

Incdence of leaf rust        None               Minor            Medium     high

Suckering ability            Poor               Medium           Good       high
                           Challenges

• Coping with:
   –   limited mother bushes of the elite varieties
   –   bureaucratic Government procurement procedures
   –   Escalating research cost
   –   escalating demand for emerging research areas-Twig borer
   –   Increasing demand on Arabica research
   –   Salaries of project staff
   –    inconsistent stakeholder support/commitment to coffee research
• Upgrading dilapidated facilities for multiplying elite
  varieties
                      Way forward

• Commitment and Consistent support from the
  stakeholder

• Increased private sector participation in technology
  development and dissemination
                  Acknowledgement

• CTF for opportunity
• All scientist involved
      •   COREC
      •   UCDA
      •   CIRAD
      •   CABI
• Uganda Government
• Farmers’ support through UCDA
• Development partners
      • EU
      • CFC/ICO
      • USAID
THANK YOU

								
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