CBRN mobile lab in Italy presentation

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					   CBRN mobile laboratories in Italy
Giorgio Mari
Italian National Institute for Safety and Health (ISPESL), via Urbana 167, Roma,
Italy
giorgio.mari@ispesl.it tel. +39 06 9789 3307



Giampaolo Giraudi, Mariarosa Bellino, Michele Pazienza
7° NBC Regiment, Italian Army, largo Acquaroni 24, Civitavecchia (RM), Italy



Claudio Garibaldi
Department of Fire and Rescue Service and Civil Defense, Fire Department of
Rome, NBCR, via Genova 3A, Roma, Italy



Corrado Lancia
Biotrace Microsafe, via Aurora 27, Fonte Nuova (RM), Italy




                   SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                          CBRN mobile laboratories in Italy

            Unconventional (NBC) terrorism threat in Rome
 Symbolic importance of the town

Religious events
                                                                  Sportive events




                                     Political events




            SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

       Sampling and approach to the laboratory.
                   (Standard NBC procedures)




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

      Main data of the mobile laboratories




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

        ITALIAN ARMY MOBILE LABORATORY




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

        ITALIAN ARMY MOBILE LABORATORY




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

        ITALIAN ARMY MOBILE LABORATORY




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                         CBRN mobile laboratories in Italy




Technical area




                                                                        - Electrical panel




                                                                      - Air-conditioner




                                                         - Hepa and carbon filters

           SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                           CBRN mobile laboratories in Italy




The shelter architecture*:


-   Technical Area (Pos.1)


-   Dressing room (Pos.2)


-   Decontamination room (Pos.3)


-   Laboratory room (Pos.4)




             SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

              ROOM PRESSURE CRITERIA




                  POTENTIALLY CONTAMINATED AREA




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                         CBRN mobile laboratories in Italy



Laboratory in a safe area:
- Internal pressure below atmospheric
- Dispersion of biological/chemical agent is prevented
- Environment is favourite
- Fire Brigades (always) and Army (standard situation)




           SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                           CBRN mobile laboratories in Italy


Laboratory in a potentially contaminated area:
- Internal pressure above atmospheric
- Penetration of biological/chemical agent from outside is prevented
- Safety of operators is favourite
- Army (emergency situation)




             SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                          CBRN mobile laboratories in Italy

 TECHNICAL SOLUTIONS WHICH ALLOW THE PRESERVATION OF THE
     SAFETY OF THE LAB OPERATORS AND OF THE EXTERNAL
               ENVIRONMENT AT THE SAME TIME

1.
The laboratory shelter is constructed with a complete tightness in order
to prevent any possible leak.
2.
There is an air filtration system for both air inlet and air outlet. The
filtration includes both particulate (so called HEPA filter, High Efficiency
Particulate Airborne) and chemical (activated carbon filter).
3.
There is no contact between the team responsible for collecting
samples (wearing scafander) and the laboratory personnel. The ingress
of the samples is done in a safe way through a pass box and the
samples are delivered directly inside the isolator
4.
The internal pressure of the isolator is always below the pressure of the
room.

            SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

    PASS BOX FOR THE SAMPLES INGRESS




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

    PASS BOX FOR THE SAMPLES INGRESS




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy


            ISOLATOR – GENERAL CONCEPT




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy


ISOLATOR WITH “NEGATIVE PRESSURE SYSTEM




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                        CBRN mobile laboratories in Italy


           “NEGATIVE PRESSURE” effectiveness tests




0.5 m/s     minimum velocity of air inlet in the glove hole




          SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy

                      AIR FILTERS




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy


       AIR FILTERS SAFE REPLACEMENT




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy



Cleaning Systems




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                 CBRN mobile laboratories in Italy

Cleaning Systems




   SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                 CBRN mobile laboratories in Italy

        CONTAINERS FOR MATERIALS TRANSFER



Rapid Transport Port (RTP)
       ISO 11933 - 3




   SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                 CBRN mobile laboratories in Italy




Rapid Transport Port (RTP)
                 ISO 11933 - 3




   SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                       CBRN mobile laboratories in Italy


 Rapid Transport Port (RTP)
                        ISO 11933 - 3

1. approach                                                    2. connection



          isolator
                                 container

                                                                                    
                                                                              
                                                                                  
 glove



         SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                         CBRN mobile laboratories in Italy


Rapid Transport Port (RTP)

3. door opening                                                   4. sample transfer

                                                                   

                                                             
                                                                  
                                                                              
                                                                                      

                           
                                                                               
                                                                  




           SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                          CBRN mobile laboratories in Italy



Rapid Transport Port (RTP)
  exterior surfaces are mainteined
           uncontaminated
5. door closing                                                   6. disconnection




                                                                     
                                                             
                                                                 
                                                                                        
                                                                                             




                                                                          CLEAN
                                                                                  CLEAN
                                                                                         
             
                                                            


           SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                  CBRN mobile laboratories in Italy



tubular film for disposal




    SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                CBRN mobile laboratories in Italy




tubular film for disposal




  SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                        CBRN mobile laboratories in Italy

                 BIOLOGICAL ANALYSIS AN DETECTION




According to the STANAG 4632 ed.1 “Deployable NBC
analytical laboratory” (STAndard Nato AGreement), inside
the biological lab of the Italian Army, the biological detection
is performed with two different techniques:


DNA identification by Real-time PCR (polymerase chain
reaction) (virus, bacteria)


Antigen identification by Immunological and
elettrochemiluminescent detection (virus, bacteria and
toxin)
          SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                               CBRN mobile laboratories in Italy

                                    Real Time -PCR
Real-time PCR (polymerase chain reaction) is a laboratory technique based on the
polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted
DNA molecule that is typical of the requested agent. Through this method the operator can
follow the reaction while the amplification is still in progress using fluorescent probes. The
fluorescence is measured in real time by a LED as it is released in response to a specific
radiation from the source light generated in the thermocycler.




                 SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
              CBRN mobile laboratories in Italy



          BL2 area - Real Time PCR




SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                            CBRN mobile laboratories in Italy

                            IMMUNOLOGICAL DETECTION

The method is based on immunological reaction (antigen-antibody) with
electrochemiluminescent detection.
The antibody is linked to a chemiluminescent label (marker). If there is the reaction
antigen/antibody the complex is excited by an electrode and emits a measurable light.




              SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                           CBRN mobile laboratories in Italy

                            MATERIAL/WORK FLOW



                                              MOBILE LABORATORY

SAMPLING
TEAM
                                                                                 BL 2
            BL 3


                                                                                  PCR




                                           IMMUNOLOGICAL
                      - ALIQUOTATION       DETECTION
                      - SAMPLES
                      PREPARATION
           WASTES
           TO BE      FOR PCR
           DISPOSED




             SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
               CBRN mobile laboratories in Italy

                    CHEMICAL ANALYSIS

GC-MS (gas chromatography–mass spectrometry system)




 SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                         CBRN mobile laboratories in Italy



                RADIOLOGICAL ASSESSMENT

The mobile laboratory (fire brigades) has a high purity
Germanium (HPGe) detector cooled in a cryostat with liquid
nitrogen, which is usually kept in a front-opening lead-lined
container for the storage of Marinelli beakers. The detector is
linked to an Ortec DigiDART 16k multichannel analyzer that can
interface with a laptop PC. The softwares used are: ScintiVision-
32 Gamma-Ray Analysis with NaI and GammaVision-32 Gamma-
Ray Analysis with HPGe. The DigiDART can be used, if
necessary, directly in the field and independently of the shelter,
with battery autonomy of up to 9 hours.
For drinking water and general liquid analysis there is an LSC
(Liquid Scintillation Counter) Hidex mod Triathler with Beta and
Alpha Counter, Gamma Counter and Luminometer functions.


           SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
                          CBRN mobile laboratories in Italy

                     Others lab accessories




                                             Refrigerated
                                             Centrifuge
autoclave



                                                                          Refrigerator


                                                       hood


            SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA
   THANK YOU !




giorgio.mari@ispesl.it
tel. +39 06 9789 3307

              SPIE Defense, Security + Sensing, 13 – 17 April 2009, Orlando USA

				
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