全球信赖的迈瑞 A GLOBAL PARTNER YOU CAN RELY ON Trouble Shooting Diego Fang International Customer Service Dept. Mindray Co., Ltd. 1 Trouble Shooting Flow Yes Visible Trouble Record error Locate the source with Customer No (Mechanical stopping/noise messages on the the instruction of Service s complaints /liquid leakage Error Log report Manual /bad smell) Yes Have spare parts? No No Repair or replace Ask for RMA End Tes number from defective parts Yes t mindray OK ? Get new parts Return defective parts 2 Error Description 2.1 Error Code Classification 50000-XX-XX: PC software error 10064-XX-XX: Main unit error 10068-XX-XX: Sample unit error 10069-XX-XX: Reagent unit error 10067-XX-XX: Mixing unit error 10066-XX-XX: Temperature control unit error 10065-XX-XX: Reaction tray and photoelectric unit error Other: Alarm information 2.2 Error Level Warning Pause Stop Emergency stop Test forbidden Startup refusal 2.3 Error Code List Refer to Chapter 6 of Service Manual 3 Judging Errors on the Reaction Curve 3.1 Reaction Curve The BS-200 analyzer features in its reaction curve. The curve and reaction data shows the whole reaction process to engineers and customers, which enables users to find and solve problems more easily. Causes for almost all result-related errors can be found on the reaction curve. 3.2 Example of Reaction Curve (GGT) F C E A B 0 D 3.3 Description As shown in the reaction curve above, the vertical axis indicates the absorbance (real value = value in this curve/10000).The intervals on the vertical axis of the reaction curve is not fixed. The horizontal axis indicates the measuring cycle, which is 16 seconds for the BS-200 analyzer. A normal double-reagent reaction curve is usually divided into 6 phases: A: time when R1 is added; B: time when R1 is preheated; C: time when sample is added; D: incubation time of sample and R1; E: time when R2 is added; F: reaction time. 3.4 Phase Description Phase A: Time When R1 Is Added The point 0 indicates the cuvette blank (which is considered as the referenced absorbance for calculating ). In other words,calculating absorbance = real 0:cuvette blank absorbance – cuvette blank. 0 Phase A: Time When R1 Is Added In the first cycle, R1 is added into the reaction cuvette, so the absorbance value here is the reagent blank of R1. Phase A: Time When R1 Is Added A:Usually it ranges from 12000 to 20000 (R1 of ALT) Absorbance values at Point A vary with different reagents. If the absorbance is much smaller than the value specified in the reagent instructions, the reagent may have lapsed. On the other hand, if the absorbance at Point A exceeds 50000 (except for reagent for CO2), visually check whether there is any contamination or bubble in the reaction cuvette. Phase B: Time When R1 Is Preheated B:Usually, absorbance does not change when the reagent is heated. Phase B: Time When R1 Is Preheated Absorbance values of some reagents may have a increase/decrease of about 200 (0.02A). Phase B: Potential Problems The normal fluctuation of absorbance between two continuous cycles is no more than 50. Otherwise there may be problems as below: •Bubbles or contamination in cuvette. In this case, the absorbance fluctuates in some tests, but not all tests. •The photoelectric conversion time matches the optical path not well. Observed in all tests for a specific wavelength. •The optical path is not stable such as an aged lamp. Phase C: Time When Sample Is Added Showing the sample is added into the reaction cuvette. Phase C: Potential Problems Possible causes for inconsistent absorbance values: Liquid dropping from probe; level detection failure for sample; In the same reduplicate fluid tubing leakage; tests,different sample malfunction of the mixing bar; volume makes different over high position of the absorbance on point C. mixing bar; unfixed position of the sample syringe. Phase D: Incubation Time The reaction curve in this If fluctuation of absorbance exceeds phase is supposed to be 50 between two continuous cycles, smooth or changing smoothly. check whether the mixing bar strikes the cuvette. Phase E: Time When R2 Is Added Similar situation as on point A and point C. Point E is also considered as the starting time of the reaction in the software. The possible problems here may be: Failure of adding R2; Bubbles or contamination with R2; Mixing bar strikes the cuvette. Phase F: Reaction Time Kinetic Endpoint Decline Incline Phase F: Potential Problems Most problems here will appear somewhere on the same reaction curve, and the causes are also similar. Such as the fluctuation caused by mixing bar ,or insufficient response as a result of the LLD failure. The impact of R2 may be considered when there is nothing abnormal on the other parts of the reaction curve. 3.5 Reaction Curve Review Even all the problems of the instruments and the reagents will result in an abnormal reaction curve. But some abnormal reaction curves seem normal. Different reaction type, different curve shape. So a nearly invisible fluctuation here may impact other tests much. Watch the instruction of the reagent carefully. How to know? Repeat the tests is very important. It can help you find the difference of the reaction curves. That is where the problems are. 4 Abnormal Test Result All results abnormal; Item results abnormal; Accidental results abnormal. Application of reaction curve! 4.1 All Results Abnormal Symptoms: All results are low; Some are high, and some are low, resulting in bad repeatability. Practice:All Results Low How do you think about the reaction curve like if there are: The added sample and reagent is insufficient (due to blocked probe, fluid tubing leakage and/or syringe leakage); The reaction is not thorough enough (because the mixing bar is too high, and/or the mixing bar does not work). Practice:Some High,Some Low • How do you think about the reaction curve like if : The mixing bar strikes the cuvette; Reagent usually has bubbles; The lamp is aged, the filter is aged, the photometric system is dirty, and/or the photoelectric conversion position is incorrect. 4.2 Item Results Abnormal In case of such an error, check the repeatability of the test. Find the rules on the reaction curves. The specific reagent deteriorated; Optical path problems of the specific wavelength; Photoelectric conversion position problems of the specific wavelength; Wrong calibration. 4.3 Accidental Results Abnormal If the results are normal in the repeated tests, the possible cause is dirty reaction cuvette or bubbles aspirated into the reagent probe. If the results are still abnormal, the possible cause exist in the sample. 5 Liquid Level Detection(LLD) Error There are three LLD errors: LLD error in reagent disc; LLD error in sample disc; LLD error in washing pool. 5.1 LLD Error: Reagent liquid level The probe fails to detect the reagent liquid level : A) Liquid level detection signal error Symptom: The detection indicator is off or always on. Abnormal assembly: Main control board or connection cable (The 12V working voltage is unavailable); reagent probe(broken or over high position); disabled LLD board. B) Transmission error Symptom: There is an alarm even when the detection indicator is on. Abnormal assembly: Probe connection cable is disconnected or in ill contact. C) Processing error Symptom: The indicator is on, and the connection cable is enabled. Abnormal assembly: Main control board. 5.2 LLD Error: Sample level Two symptoms : A:failing to detect liquid level in sample tray; B:failing to detect liquid level in reaction tray. The same causes as Both A and B: that of the reagent part(chapter 5.1) No/insufficient The probe mistakes to Only B: reagent in the detect the liquid level. cuvette 5.3 LLD Error of The Probe in Washing Pool Two symptoms : No water in washing pool; water available in washing pool. No water in washing pool The distill water container is empty (the sensor is disabled); The exterior-washing pump is disabled (no water in all pools); The tubing is blocked (single-way valve blocked, 3-way connector blocked); The tubing is disconnected. Water available in washing pool The same as that of the probe failing to detect liquid level in the reagent part. 5.4 LLD Error Caused by Little Tubes The capacity of liquid aspirated only depend on the depth of the probe under the liquid level. Different area of cross section, different capacity. Using little tube instead of standard tube will cause insufficient sample /reagent. 6 Liquid Dropping From Probes • Three possible causes: Fluid tubing leakage (syringe/connector/valve) LLD error Other problems (dirty probes/unqualified probe driver assembly and so on) Cause1: Fluid Tubing Leakage Distinct symptoms: In this case, the liquid drop in a large scale, some of which are relatively big, and dropping happens even when the probes are still. Solution: Check the syringe piston/tubing connector/valves on the leaking side and repair/replace the defective ones. Cause2: LLD Error Distinct symptoms: In this case, most drops are found between the reaction tray and the washing pool. Solution: Correct the over high position of the rocker-arm; Be sure there is no bubble in the reagent bottle and the cuvette; Be sure that the probe is in the center while dispensing liquid; Tight the screw for fixing the syringe . Note:The probe will NOT detect liquid level in the reaction tray when add reagent. Cause3: Other Problems A) Distorted probe tip Cause: The probe tip is distorted, resulting in carryover. Solution: Replace the probe. B) Dirty exterior of the probe Cause: Contaminated by samples, resulting in carryover. Solution: Clean or replace the probe. C) Unqualified probe driver assemblies Cause: The probe driver assemblies are unqualified, resulting in severe jitters at the moment when the probe is rising. Solution: Replace the defective ones. 7 Problems in Photoelectric Unit Insufficient Light Intensity: A) Optical path Symptom: Low background of several wavelengths, specially of the short wavelengths. Solution: Clean the end face of the optical fiber. And increase the gain if it is too low yet. B) Aged lamp Symptom: Either of the background of the 340nm wavelength or reference wavelength is lower than 43000. Solution: Replace the lamp. Insufficient Light Intensity 2 C) Aged filter Symptom: After the lamp is replaced, the background of a certain wavelength is still very low. Solution: Replace the defective pre-amplification assembly. D) Defective pre-amplification board Symptom: The background of a certain wavelength is nearly 0. Solution: Replace the defective pre-amplification board. Photoelectric Conversion Position Match Not Well B: Indicate the time of A/D converting. Cuvette center Air between A: Photoelectric signal Cuvette wall cuvettes of a certain wavelength. 8 Temperature Control Error Only the reagent preheating has an alarm. Causes: The preheating temperature transducer is disconnected; The preheating temperature transducer does not work; The preheating system is switched off. Both the reagent preheating and reaction tray have alarms. Causes: Temperature control parameters are lost. Solution for This Error Monitoring the temperature curve; Correct the temperature control parameters using the Parameter Configuration List; Measuring the impedance of the temperature transducer, and replace the disabled one; Replace the heater. Summary Here we can only give you a suggestion on how to find the problems. Actually the problems you facing always result in multiform symptoms. Do observe the reaction curve carefully, and find out the roots of the errors. Most alarm information of the BS-200 analyzer is obtained based on sensor signals, so it is necessary to learn the signal flows. Note: Backup the important data and parameters when you prepare to replace some parts. Thanks!
Pages to are hidden for
"Mindray BS 200 Trouble Shooting"Please download to view full document