The proline accumulation in salt tolerant plants

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					   The proline accumulation in
       salt tolerant plants


Elferra Swart, Maarten Weerenbeck en David van Diepen
                             Proline
• Amino acid + compatible osmolyte
  – Remain unchanged, H2O-soluble, no effect on other molecules

• First plants response water stress:
  – decrease growth rate and turgor + prevention of feedback inhibition
    proline synthesis. (Crawford, 1989)

• protects membranes and proteins against
  dangers of dehydration
• Accumulation in cytosol leaves in salt tollerant
  plants, but also other plants during salt stress..
              Hypothesis
• Proline accumulates when salt concentration
  becomes higher
                 Method
• Proline can be made visible by reaction
  with ninhydrine ( purple complex)
• Extinction muesurement, ED 520 nm
• Making standard line with known
  concentration
• Calculation proline amount
                                                                Results

                      A. thaliana , proline concentration                                       T. salsugenia, proline concentration

                     1                                                                     16
                                                                                           14




                                                                       Extinction / g FW
Extinction / g FW




                    0,8                                                                    12
                    0,6                                                                    10
                                                                                            8
                    0,4                                                                     6
                                                                                            4
                    0,2
                                                                                            2
                     0                                                                      0
                          0                  50                  100                            0             50                100       150
                                    salt concentration                                                        salt concentration


                                individual outcomes   average                                             individual outcomes   average
                                                                       Results

                            C. danica , proline concentration                                                   T.h.altai, proline concentration

                    7                                                                                  16
                    6                                                                                  14




                                                                                   Extinction / g FW
Extinction / g FW




                    5                                                                                  12
                                                                                                       10
                    4
                                                                                                        8
                    3
                                                                                                        6
                    2                                                                                   4
                    1                                                                                   2
                    0                                                                                   0
                        0     50   100   150   200   250   300   350   400   450                            0               50                100       150
                                          salt concentration                                                                salt concentration


                                     individual outcomes   average                                                      individual outcomes   average
                                                                      Results

                         C. anglica, proline concentration                                                T. burriata, proline concentration

                    25                                                                                6




                                                                                  Extinction / g FW
Extinction / g FW




                    20                                                                                5
                                                                                                      4
                    15
                                                                                                      3
                    10
                                                                                                      2
                    5                                                                                 1
                    0                                                                                 0
                         0   50   100   150   200   250   300   350   400   450                           0           50                 100       150
                                        salt concentration                                                             salt concentration


                                    individual outcomes   average                                                  individual outcomes   average
                                  Results

                            T.h. tuva , proline concentration

                    7
Extinction / g FW

                    6
                    5
                    4
                    3
                    2
                    1
                    0
                        0              50                 100       150
                                        salt concentration


                                    individual outcomes   average
                     Discussion
• No standard measures, but clear accumulation
• In every plant, proline accumulates, what’s the
  difference? Tollerant plants don’t begin at 0!
• Excellent results!                             7                                    0,2

  – Hypothese correct                            6
                                                                                      0,16
                                                 5




                                                                                             freshweight
    without doubt                                                                     0,12




                                       proline
                                                 4
                                                 3                                    0,08
                                                 2
                                                                                      0,04
                                                 1
                                                 0                                    0




                                                                 0



                                                                          0



                                                                                  0
• Further questions:                             0



                                                     50



                                                              10



                                                                       20



                                                                               40
                                                          salt concentration


  – Does proline keeps rising?            proline freshweight


  – What kind of trendline between salt and proline?
  – What is the correlation with other data?
               Conclusion
• This was a very interesting talk
• You can all applaud for me
• If you really want, I can do the talk again

          Thank you!
                   Credits:
• Deze powerpoint is gemaakt door:
  – David van Diepen
• Kijk voor meer powerpoints op:
  – www.davidvandiepen.nl




  Ik vind het leuk als u een berichtje achterlaat op
    mijn gastenboek!

				
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posted:5/21/2012
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