Infectious Waste Disposal Checklist by 6TcufY


									                   Infectious Waste Disposal Flowchart
1. Does the waste contain any radioactive materials?
      a. Yes—Dispose as RADIOACTIVE waste. Contact the Radiation Safety Officer at
          Safety and Risk Management for more information.
      b. No—Continue

2. Does the waste contain any hazardous chemicals (e.g. formaldehyde, ethidium bromide,
   polyacrylamide, heavy metals) or items with hazardous characteristics (e.g. ignitable,
   corrosive, reactive, or toxic)?
       a. Yes—Dispose as CHEMICAL waste. Contact the Chemical Safety Officer at
           Safety and Risk Management.
       b. No—Continue

3. Does the waste contain medical SHARPS or SHARPS contaminated with infectious
   organisms, genetically modified organisms, live or attenuated vaccines, rDNA organisms,
   or human blood (e.g. hypodermic needles, syringes with needles, Pasteur pipets, broken
   glass, pipette tips)?
       a. Yes—
               i. Minimize the handling of SHARPS. Needles will not be bent,
                  sheared, broken, recapped, removed from disposable syringes, or
                  otherwise manipulated by hand before disposal.
              ii. Carefully place used disposable needles and syringes in puncture-
                  resistant containers used for sharps disposal. Sharps containers
                  will not be beyond ¾ full. Contact Safety and Risk Management
                  for replacement SHARPS disposal containers at
             iii. Use a brush and dustpan or forceps to handle broken glassware.
             iv. When sharps containers are full, contact
         for disposal.
       b. No—Continue

 4. Does the waste contain non-contaminated, non-medical SHARPS (e.g. plastic
    pipette tips; microscope slides; cover slips; broken, clean glassware)?
         a. Yes—Collect non-contaminated SHARPS in a glass disposal box. These
             can be obtained from Safety and Risk Management. Close and tape the
             box shut when it is ¾ full. Dispose of Box in the trash.
         b. No—Continue

 5. Does the waste contain animal carcasses, tissues, or organs that have been
    contaminated with infectious organisms or rDNA?
        a. Yes—Contact the Animal Resources Center Director or Manager
        b. No—Continue

  6. Does the waste contain cultures of infectious organisms, genetically altered
     organisms, live or attenuated vaccines, or rDNA organisms?
        a. Yes-Separate liquid cultures from other contaminated waste.
                    i.   Liquid cultures may be chemically or physically

                             a. Chemical disinfection.
                             Before disinfection: Choose a disinfectant that has been
                             proven to kill the microorganism you are working with.
                             Follow the manufacturer’s recommendations for handling
                             the disinfectant and wear appropriate protection. Contact
                             the Chemical Safety Officer to determine if the disinfectant
                             can be poured into a sanitary sewer. To disinfect a culture,
                             add the amount of concentrated disinfectant into the culture
                             so that the final use-dilution will be that which is
                             recommended. Allow the culture-disinfectant solution to sit
                             for the manufacturer’s recommended length of time for
                             disinfection. After the appropriate contact time, the culture
                             can be disposed of in the sanitary sewer (drain). If you have
                             a heavy bioburden, you may have to increase the
                             concentration of disinfectant.

                             a. Physical decontamination—steam sterilization in an
                             Autoclave liquid cultures in a spore-tested autoclave at an
                             approved temperature for the appropriate amount of time
                             using a liquid autoclave cycle. Sterilized cultures may be
                             disposed down the drain into a sanitary sewer.

                   ii.   Other waste: agar plates, culture tubes, paper towels, bench
                         paper, gloves, etc.

                         Collect the waste in high strength polymer autoclave bags. Do
                         not place waste into the bags that may poke through such as
                         applicator sticks, Pasteur pipets, or swabs. Label each bag with
                         autoclave tape and/or a chemical indicator strip. Autoclave the
                         waste in a spore-tested autoclave at the approved temperature
                         for the appropriate time. Utilize autoclavable containers and
                         procedures developed for your department. After autoclaved
                         waste has cooled, dispose of in the regular trash.

           b. No—Continue

7. Does your waste require incineration?
      a. Yes—Contact Safety and Risk Management
      b. No—continue

8. If the answer to each item above is NO, then trash disposal is permitted!

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