In vitro induction of a trisomic of Agave tequilana Weber var. Azul (Agavaceae) by para-fluorophenylalanine treatment

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In vitro induction of a trisomic of Agave tequilana Weber var. Azul (Agavaceae) by para-fluorophenylalanine treatment Powered By Docstoc
					In Vitro Cell.Dev.Biol.—Plant (2012) 48:144–152
DOI 10.1007/s11627-011-9405-0

 SOMATIC CELL GENETICS



In vitro induction of a trisomic of Agave tequilana Weber
var. Azul (Agavaceae) by para-fluorophenylalanine
treatment
Domingo Ruvalcaba-Ruíz & Guadalupe Palomino &
Javier Martínez & Ignacio Méndez &
Benjamín Rodríguez-Garay



Received: 21 December 2010 / Accepted: 5 September 2011 / Published online: 6 October 2011 / Editor: J. Finer
# The Society for In Vitro Biology 2011


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DOCUMENT INFO
Description: The effect of para-fluorophenylalanine (PFP) on the production of trisomic plants of Agave tequilana Weber var. Azul produced through somatic embryogenesis was investigated. Normal diploid plants with 2n=2x=60 were obtained in the control treatment and with 4 mg L^sup -1^ PFP exposure, while use of 8 and 12 mg L^sup -1^ PFP led to production of trisomics with 2n=2x=61. Normal diploid plants showed a bimodal karyotype with five pairs of large chromosomes and 25 pairs of small chromosomes. Trisomic plants also had a bimodal karyotype with a group of three chromosomes in position five of the chromosome set. More than 13 homologous chromosome pairs exhibited structural changes. Differences in chromosome arm ratio (long arm/short arm) were also found in eight chromosome pairs; all these aberrations in the chromosome complement of trisomic plants were probably caused by inversions, deletions, and/or duplications produced by high concentrations of PFP. The gross chromosome structural changes and the presence of a single extra chromosome could have been induced by the effect of PFP on the mitotic spindle by inducing nondisjunction of sister chromatids, resulting in hyperploids (2n + x) and hypoploids (2n - x). Flow cytometric analysis of nuclear DNA content was performed using nuclei isolated from young leaves of normal and trisomic plants. The 2C DNA content of 8.635 pg (1Cx= 4,223 Mbp of trisomic plants was different (p0.001) than that of normal plants (2C DNA=8.389 pg (1Cx= 4,102 Mbp). The difference in genome size was correlated with the large structural changes in the trisomic plant genomes. [PUBLICATION ABSTRACT]
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