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Device For The Actively-controlled And Localised Deposition Of At Least One Biological Solution - Patent 8079832

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Device For The Actively-controlled And Localised Deposition Of At Least One Biological Solution - Patent 8079832 Powered By Docstoc
					
				
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Description: This application is a national stage application filed under 35 U.S.C. 371 of International Application No. PCT/FR03/01481, filed May 15, 2003, which claims priority from French Application No. FR0206016, filed May 16, 2002.FIELD AND BACKGROUND OF THE INVENTION The subject of the present invention is a device for the actively controlled, localized deposition of at least one biological solution in the form of microdrops. In the pharmaceutical industry, the capital invested in research for developing new medicines occupies a considerable portion of an enterprise's budget. New assay methods are needed to reduce the cost of such research. The arrival of microchips in the biomedical sector has revolutionized the fields of medicinal product development and of bioassay. The advantages of these microchips are the following: they allow new, more sensitive detection methods to be developed; they require smaller volumes of reactants, hence a lower cost; they allow analytical procedures that are more rapid, owing to their small dimensions; and they allow screening ordiagnostic studies to be carried out owing to the large number of different solutions present on any one surface. However, the tools that are currently operational for distributing small volumes of biological material in solution allow the deposition, on glass slides or on membranes, of drops with a diameter of the order of a hundred microns (whichcorresponds to a drop volume of the order of a nanoliter). These systems rely: either, in a first case, on a piezoelectric active device for sucking up and ejecting the products in solution (a contactless deposition system); or, in a second case, on apassive mechanism consisting of split pins made of metal (stainless steel, tungsten, etc.), the liquid being sucked up in this second case by capillary effect and being deposited by bringing the end of the pin into contact with a glass slide (a contactdeposition system). We should also mention the pin-and-ring system, the