The Yeast Nuclear Pore Complex and Transport Through It by ProQuest


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The Yeast Nuclear Pore Complex and Transport
Through It
John D. Aitchison*,1 and Michael P. Rout†
*Institute for Systems Biology and Seattle Biomedical Research Institute, Seattle, Washington 98109, and †The Laboratory of Cellular and Structural
Biology, The Rockefeller University, New York, New York 10021

ABSTRACT Exchange of macromolecules between the nucleus and cytoplasm is a key regulatory event in the expression of a cell’s
genome. This exchange requires a dedicated transport system: (1) nuclear pore complexes (NPCs), embedded in the nuclear envelope
and composed of proteins termed nucleoporins (or “Nups”), and (2) nuclear transport factors that recognize the cargoes to be
transported and ferry them across the NPCs. This transport is regulated at multiple levels, and the NPC itself also plays a key regulatory
role in gene expression by influencing nuclear architecture and acting as a point of control for various nuclear processes. Here we
summarize how the yeast Saccharomyces has been used extensively as a model system to understand the fundamental and highly
conserved features of this transport system, revealing the structure and function of the NPC; the NPC’s role in the regulation of gene
expression; and the interactions of transport factors with their cargoes, regulatory factors, and specific nucleoporins.

    Abstract                                                                                                                                                        855
    Introduction                                                                                                                                                    856
    Structure and Composition of the NPC                                                                                                                            856
    Overall Composition                                          
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