Docstoc

8_10_strep_data_for_needle_gliadin_eild_kilr_inp

Document Sample
8_10_strep_data_for_needle_gliadin_eild_kilr_inp Powered By Docstoc
					SAFIRE II; Serial number: 807003897; Firmware: V 2.10 12/2007 Safire2; XFLUOR4SAFIREII Version: V 4.62n
Date:
Time:

Measurement mode:
Excitation wavelength:
Emission wavelength:
Excitation bandwidth:
Emission bandwidth:
Gain (Manual):
Number of reads:
FlashMode:
Integration time:
Lag time:
Plate definition file:
Z-Position (Manual):
Shake duration (Linear Medium):
Target Temperature:
Current Temperature:

Rawdata (RFU)
            <>                              1               2           3               4
              A                            93              81          82              86
              B                            82              78          75              78
             C                             92              76          69              66
             D                             73              76          24              73
              E                            76              78          25              74
              F                            54              50          26              71
             G                             27              25          24              23
             H                             25              26          23              25

{<cl02365 AtD>}                   {<Pcryo_1225 AtD>}    {<ehaB>} {<VtaA11 AtD>} {<OprF AtD>}
              93                           81               82         86              75
              82                           78               75         78              82
              92                           76               69         66              68




not normalized averaged
                            89                     78           75           77                75

average of PBS
                            25

subtracted PBS background
                           64                      53       50                52               50
                   6.08276253             2.516611478 6.506407       10.06644591                7

                                           averaged PBS background subtracted
                  3500
                  3000
                  2500
   fluorescence




                  2000
                  1500
                  1000
                   500
                     0
                         1   2   3   4     5   6    7    8   9 10 11 12 13 14 15 16 17 18 19 20 21
                  -500
                                                                      constructs




ODs after 3 washes
{<cl02365 AtD>}                          {<Pcryo_1225 AtD>}       {<ehaB>} {<VtaA11 AtD>} {<OprF AtD>}
             0.3176                             0.4043              0.4102      0.4236         0.3541
             0.3326                             0.4120              0.4061      0.3989         0.4247
             0.2980                             0.3565              0.3555      0.3531         0.3350

                  averaged ODs
                     0.3161                     0.3909             0.3906      0.3919          0.3713

subtracted by PBS background
                       0.2661                            0.3409      0.3406         0.3419          0.3213

OD normalized
                          240.5412177              156.4333203 147.7784       151.1310452       155.633949


                                                    OD normalized averaged

  20000
  18000
  16000
  14000
  12000
  10000
   8000
   6000
   4000
   2000
2000
   0
       1   2   3   4   5   6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
REII Version: V 4.62n
                        7/8/09
                        17:57

             Fluorescence
                         488     nm
                         575     nm
                           20    nm
                           20    nm
                           50
                           20
             High sensitivity
                           40    µs
                            0    µs
             COS96ft.pdf
                       5100      µm
                            1    s
                           37    °C
                           37    °C

             Temperature:                                                       37 °C
                   5                     6                7              8                9                10
                  75                    70               64             65               25                65
                  82                    74               72             68               23                63
                  68                    70               66             63               25                55
                 2806                  3220              55             54               55                48
                 2733                  3194              52             53               45                47
                 2818                  3345              60             51               56                50
                  25                    25               26             24               27                23
                  24                    25               24             23               25                25

             {<Hag AtD>}         {<Hia AtD>}        {<upaG_short>} {<CPG_L6>}        {<eCPX>}        {<yuaQ AtD>}
                   70                   64                65            25               65                53
                   74                   72                68            23               63                54
                   70                   66                63            25               55                50




                           71                  67              65               24              61                  52




                        46                     42               40            -1         36                      27
               2.309401077            4.163331999      2.516611478   1.154700538 5.29150262             2.081665999

                                                                                                                averaged PBS backgrou
                                                                     100
                                                                      90
                                                                      80
                                                                      70




                                                      fluorescence
                                                                      60
                                                                      50
                     Series1
                                                                      40
                                                                      30
                                                                      20
                                                                      10
                                                                       0
21 22 23 24
                                                                     -10       1   2     3      4   5     6      7   8



                                                   1-6, 16


{<Hag AtD>}     {<Hia AtD>}       {<upaG_short>} {<CPG_L6>}                    {<eCPX>}         {<yuaQ AtD>}
    0.3827           0.3951           0.4041         0.0447                       0.4110             0.3805
    0.4254           0.4384           0.4013         0.0449                       0.3949             0.4354
    0.3611           0.3705           0.3855         0.0585                       0.3766             0.3503


   0.3897           0.4013            0.3970                 0.0494                0.3942               0.3887


       0.3397            0.3513           0.3470                     -0.0006           0.3442                 0.3387


  136.3814757      120.4933586       116.2455567                               104.600484           80.69277701


                                                                                          OD normalized averaged

                                   300

                                   250

                                   200

     Series1                       150

                                   100

                                     50
     0
24       1   2   3   4   5   6   7   8   9 10 11 12 13 14 15
         11             12
         53             51
         54             57
         50             50
         54             48
         55             50
         53             51
         26             25
         24             24

   {<azo1653 AtD>} {<CPG_L2>}      {<espP(beta)>}        {<TshA>}        {<AIDA-1 AtD>} pos induced 9495
          51            73                  76                24               73              2806
          57            76                  78                25               74              2733
          50            54                  50                26               71              2818




               53            68                     68              25               73              2786




                28            43                    43              0                48              2761
       3.785938897   11.93035345           15.62049935              1       1.527525232       46.00362305

averaged PBS background subtracted
                                                                                        Series1




        8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
                    constructs




         {<azo1653 AtD>} {<CPG_L2>}        {<espP(beta)>}            {<TshA>}      {<AIDA-1 AtD>} pos induced 9495
              0.3363        0.3097                0.3270                0.0428         0.3514            0.2178
              0.3646        0.3196                0.3353                0.0470         0.3660            0.2111
              0.2982        0.3243                0.3258                0.0654         0.3813            0.2178


             0.3330           0.3179              0.3294               0.0517          0.3662           0.2156


                    0.2830        0.2679                    0.2794        0.0017           0.3162             0.1656


             97.75055942     159.2832255             153.91958                        150.7325814       16674.04872


rmalized averaged




                                                     Series1
14 15 16 17 18 19 20 21 22 23 24
pos induced 9145    neg induced    pos unin 9495        pos unin 9145 neg unin    {<cl02365 AtD>} unin
       3220               55              54                  55          48               54
       3194               52              53                  45          47               55
       3345               60              51                  56          50               53




             3253             56                   53              52        48                     54




            3228          31                   28                  27       23                      29
     80.72793816 4.041451884          1.527525232          6.08276253 1.527525                       1
pos induced 9145    neg induced    pos unin 9495     pos unin 9145 neg unin    {<cl02365 AtD>} unin
      0.2267           0.2426           0.2381           0.2720      0.2673           0.2554
      0.2326           0.2499           0.2348           0.2775      0.2373           0.2513
      0.2412           0.2521           0.2224           0.2710      0.2473           0.2425


     0.2335            0.2482          0.2318           0.2735       0.2506           0.2497


           0.1835         0.1982            0.1818          0.2235    0.2006                   0.1997


     17591.28065 154.7258661          152.2097928     120.8053691 116.2984             145.1935915
{<Pcryo_1225 AtD>} unin
           48
           50
           51




                      50




                     25
            1.527525232
{<Pcryo_1225 AtD>} unin
         0.2626
         0.2158
         0.2240


         0.2341


                  0.1841


            133.9608979
SAFIRE II; Serial number: 807003897; Firmware: V 2.10 12/2007 Safire2; XFLUOR4SAFIREII Version: V 4.62n
Date:
Time:

Measurement mode:
Excitation wavelength:
Emission wavelength:
Excitation bandwidth:
Emission bandwidth:
Gain (Manual):
Number of reads:
FlashMode:
Integration time:
Lag time:
Plate definition file:
Z-Position (Manual):
Shake duration (Linear Medium):
Target Temperature:
Current Temperature:

Rawdata (RFU)
         <>                        1                 2                 3              4
         A                        46                30                54             48
         B                        27                41                44             48
         C                        44                45                44             46
         D                        22                50                48             56
         E                        25                29                45             51
         F                        22                59                46             54
         G                        23                24                21             23
         H                        27                22                22             23


{<cl02365 AtD>}           {<Pcryo_1225 AtD>}    {<ehaB>}        {<VtaA11 AtD>} {<OprF AtD>}
         46                        30                54               48             74
         27                        41                44               48             48
         44                        45                44               46             56


not normalized avg
                     39                    39              47               47                59

subtract by PBS
average                                    16            16                 24             24
standard dev                      10.44030651    7.76745347        5.773502692    1.154700538

                                        averaged unnormalized

  3500

  3000

  2500
  2500

  2000

  1500

  1000

   500

      0
          1    2   3    4    5    6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
   -500




OD after 3 washes
{<cl02365 AtD>}             {<Pcryo_1225 AtD>}         {<ehaB>}      {<VtaA11 AtD>} {<OprF AtD>}
       0.2884                      0.2734                 0.2435          0.2743        0.3036
       0.3049                      0.3005                 0.2594          0.2848        0.2938
       0.2437                      0.2583                 0.2268          0.2611        0.2577


averaged ODs
               0.2790                         0.2774        0.2432           0.2734          0.2850

OD subtracted by
background
              0.2290                          0.2274        0.1932           0.2234          0.2350

OD normalized
       69.86899563                    68.89475227       125.927204      108.9227096    154.5880017


                                                   OD normalized

  25000

  20000

  15000

  10000

   5000

      0
          1    2    3   4     5   6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
OR4SAFIREII Version: V 4.62n
                     7/8/09
                      18:00

            Fluorescence
                          488    nm
                          575    nm
                           20    nm
                           20    nm
                           50
                           20
            High sensitivity
                           40    µs
                             0   µs
            COS96ft.pdf
                        5100     µm
                             1   s
                           37    °C
                           37    °C

            Temperature:                                                           37 °C
                  5                       6                 7                8               9            10
                 74                      61                55               53             3322           56
                 48                      55                48               47             3022           50
                 56                      47                50               49             2715           52
                 50                      65                22               58              56            57
                 54                      68                23               59              54            58
                 54                      65                22               55              53            56
                 22                      23                23               22              22            23
                 23                      25                24               22              24            22


            {<Hag AtD>}          {<Hia AtD>}        {<upaG_short>}    {<CPG_L6>}        {<eCPX>} {<yuaQ AtD>}
                  61                    55                53                22              50         48
                  55                    48                47                25              29         45
                  47                    50                49                22              59         46
                                                                                   23


                           54                  51                50                23             46           46


                        36                     31                28               27        0                   23
               13.31665624            7.023769169       3.605551275      3.055050463 1.732051          15.39480432

                                                                                        averaged unnormalized

                                                     100
                                                      90
                                                      80
                                                      70
                                      70
                                      60
                                      50
   Series1
                                      40
                                      30
                                      20
                                      10
                                       0
                                     -10       1       2       3       4   5   6   7    8   9 10 11 12 13 14 15 16


                                   of interest: 14


{<Hag AtD>}       {<Hia AtD>}      {<upaG_short>}                  {<CPG_L6>}               {<eCPX>} {<yuaQ AtD>}
    0.3082            0.3022            0.3115                         0.0472                 0.2229     0.2700
    0.3141            0.3175            0.3113                         0.0576                 0.2572     0.2918
    0.2720            0.2693            0.2808                         0.0484                 0.2339     0.2842



         0.2981           0.2963                   0.3012                          0.0511      0.2380        0.2820



         0.2481           0.2463                   0.2512                          0.0011      0.1880        0.2320


   126.2931614      113.6671177         106.1571125                                         122.3404    100.5747126


                                                                                                   OD normalized

                                     1000
                                      900
                                      800
                                      700
                                      600
   Series1                            500
                                      400
                                      300
                                      200
                                      100
                                           0
                                                   1       2       3   4   5   6    7   8    9 10 11 12 13 14 15 16
                 11            12
                 57            60
                 51            45
                 46            45
                3350           42
                3023           46
                3342           47
                 23            24
                 22            23


         {<azo1653 AtD>} {<CPG_L2>}        {<espP(beta)>} {<TshA>} {<AIDA-1 AtD>}        pos induced 9495
                56             50                65           22          58                    3322
                51             54                68           23          59                    3022
                54             54                65           22          55                    2715



                       54             53              66        22                  57                3020


                        23            31              30       43                -1                     34
               1.527525232   2.516611478     2.309401077 1.732051       0.577350269            2.081665999

unnormalized
                               Series1




16 17 18 19 20 21 22 23 24




 {<azo1653 AtD>} {<CPG_L2>}        {<espP(beta)>} {<TshA>} {<AIDA-1 AtD>}     pos induced 9495
      0.1128          0.0590           0.2302       0.0426      0.2616              0.1962
      0.1179          0.0474           0.2175       0.0490      0.2282              0.1921
      0.1163          0.0492           0.2084       0.0459      0.2253              0.1763
                 no growth?


          0.1157          0.0519          0.2187    0.0458           0.2384              0.1882



          0.0657          0.0019          0.1687   -0.0042           0.1884              0.1382


     467.0050761                     254.8903379                 182.268625         21683.55041




                               Series1




16 17 18 19 20 21 22 23 24
pos induced 9145          neg induced        pos unin 9495        pos unin 9145        neg unin
         3350                    56                 57                   42                60
         3023                    50                 51                   46                45
         3342                    52                 46                   47                45



                   3238                 53                   51                   45              50


                2997                 3215                30                   28        22
          303.506727          186.5270311       3.055050463          5.507570547 2.6457513
pos induced 9145        neg induced        pos unin 9495     pos unin 9145     neg unin
        0.2203                0.2285            0.2113            0.2227         0.2159
        0.2031                0.2115            0.1998            0.2317         0.2166
        0.2149                0.2018            0.1959            0.2391         0.2049



               0.2128             0.2139            0.2023            0.2312       0.2125



               0.1628             0.1639            0.1523            0.1812       0.1625


         19754.24944        180.9678731       185.9956236       121.4351426 166.18794
{<cl02365 AtD>} unin {<Pcryo_1225 AtD>} unin
         56                     57
         54                     58
         53                     56



                  54                       57


                 27                        31   34
        8.660254038               1.527525232    1
{<cl02365 AtD>} unin {<Pcryo_1225 AtD>} unin
       0.1927                 0.1791
       0.1927                 0.2034
       0.1989                 0.2051



              0.1948                   0.1959



              0.1448                   0.1459


        216.4402487               233.0895795
SAFIRE II; Serial number: 807003897; Firmware: V 2.10 12/2007 Safire2; XFLUOR4SAFIREII Version: V 4.62n
Date:                                                                                     8/8/09
Time:                                                                                      20:59

Measurement mode:                                                                         Fluorescence
Excitation wavelength:                                                                                 488
Emission wavelength:                                                                                   575
Excitation bandwidth:                                                                                    20
Emission bandwidth:                                                                                      20
Gain (Manual):                                                                                           50
Number of reads:                                                                                         20
FlashMode:                                                                                High sensitivity
Integration time:                                                                                        40
Lag time:                                                                                                  0
Plate definition file:                                                                    COS96ft.pdf
Z-Position (Manual):                                                                                 5100
Shake duration (Linear Medium):                                                                            5
Target Temperature:                                                                                      37
Current Temperature:                                                                                     37

Rawdata (RFU)                                                                             Temperature:
        <>                       1                   2            3             4               5
         A                      47                  85           53           313             7636
         B                      42                  65           41           226             4963
         C                      45                  61           46           200             4953
         D                      42                 161          203            83              27
         E                      41                 170          191            87              27
         F                      38                 161          195            85              27
         G                      23                  25           26            26              25
         H                      27                  27           25            28              26


{<cl02365 AtD>}        {<Pcryo_1225 AtD>}        {<ehaB>} {<VtaA11 AtD>} {<OprF AtD>}     {<Hag AtD>}
         47                     85                   53         313            42              161
         42                     65                   41         226            41              170
         45                     61                   46         200            38              161
average
                  45                        70           47           246           40                 164
subtract background
                  18                    43       20                   219            13              137
        2.516611478            12.85820101 6.027714           59.18051481   2.081665999      5.196152423

  7000

  6000

  5000

  4000

  3000                                                                                             Series1

  2000
  2000

  1000

      0
             1   2   3    4    5   6    7    8    9 10 11 12 13 14 15 16 17 18 19 20 21
  -1000




OD after 3rd wash
{<cl02365 AtD>}          {<Pcryo_1225 AtD>}           {<ehaB>} {<VtaA11 AtD>} {<OprF AtD>}     {<Hag AtD>}
       0.1899                   0.2845                  0.2384      0.2874        0.2813           0.2682
       0.1879                   0.2921                  0.2589      0.2771        0.2879           0.2861
       0.1982                   0.2441                  0.2491      0.2676        0.2843           0.2816
      averaged
       0.1920                      0.2736              0.2488      0.2774         0.2845           0.2786

subtract by PBS
background
              0.1520                         0.2336      0.2088        0.2374         0.2445           0.2386



OD normalize fluor
       116.2280702                     185.528757 94.18902        924.0275242   54.53306067      574.1025283


                                                   OD normalized

  35000

  30000

  25000

  20000
                                                                                                       Series1
  15000

  10000

   5000

         0
             1   2   3     4   5    6    7    8   9 10 11 12 13 14 15 16 17 18 19 20 21
II Version: V 4.62n




              nm
              nm
              nm
              nm



              µs
              µs

              µm
              s
              °C
              °C

                                                                  37 °C
                        6                  7               8                   9               10
                      6899                48              43                  51               26
                      5586                44              43                  40               25
                      4875               105              40                  39               26
                       41                 40              42                  39               36
                       42                 39              39                  43               38
                       40                 38              36                  44               37
                       27                 26              27                  25               25
                       27                 26              27                  27               27


              {<Hia AtD>}          {<upaG_short>}    {<CPG_L6>}        {<eCPX>}         {<yuaQ AtD>}
                     203                 83               27                  41               40
                     191                 87               27                  42               39
                     195                 85               27                  40               38

                             196                85                27               41                  39

                           169                  58                 0               14                  12
                   6.110100927                   2                 0                1                   1

                                     1000
                                      900
                                      800
                                      700
                                      600
                                      500
    Series1
                                      400
                                      300
                                300
                                200
                                100
                                   0
                                       1    2   3   4     5      6   7    8   9 10 11 12 13 14 15 16 17
                                -100


                             4, 6, 7



          {<Hia AtD>}        {<upaG_short>}     {<CPG_L6>}               {<eCPX>}            {<yuaQ AtD>}
                0.2780            0.2628            0.0363                    0.2614              0.2633
                0.3044            0.2800            0.0385                    0.2625              0.2571
                0.3037            0.2694            0.0390                    0.2588              0.2465

               0.2954              0.2707               0.0379                0.2609              0.2556



                    0.2554             0.2307                 -0.0021               0.2209             0.2156




              663.0988122         251.3724357                                  63.37709371       55.65002319



                                                                               OD normalized

                               1000
                                900
                                800
                                700
                                600
Series1                         500
                                400
                                300
                                200
                                100
                                  0
                                       1    2   3   4     5     6    7    8   9 10 11 12 13 14 15 16 17
       11                    12
       25                    26
       27                    27
       24                    25
       26                    37
       27                    40
       27                    40
       26                    27
       27                    26


{<azo1653 AtD>}        {<CPG_L2>}        {<espP(beta)>}        {<TshA>}        {<AIDA-1 AtD>}
       42                   39                    36                26               37
       39                   43                    38                27               40
       36                   44                    37                27               40

                  39                42                    37              27                39

                  12              15                      10              0                12
                   3     2.645751311                       1    0.577350269       1.732050808




                        Series1
17 18 19 20 21




  {<azo1653 AtD>}     {<CPG_L2>}          {<espP(beta)>}            {<TshA>}        {<AIDA-1 AtD>}
        0.2371            0.2516                 0.2769                 0.0366           0.2391
        0.2587            0.2691                 0.2777                 0.0376           0.2493
        0.2460            0.3012                 0.2886                 0.0383           0.2533

       0.2473             0.2740                 0.2811                0.0375           0.2472



             0.2073              0.2340                    0.2411         -0.0025            0.2072




       57.89642972      64.11169682                41.48230088                         57.90574232




                       Series1




17 18 19 20 21
pos induced 9495    pos induced 9145 neg induced        pos unin 9495        pos unin 9145
       7636                6899              48                 43                 51
       4963                5586              44                 43                 40
       4953                4875             105                 40                 39

             5851              5787                66                   42               43

             5824              5760                39                 15                16
      1546.152105       1026.812706       34.12232896        1.732050808       6.658328118
pos induced 9495    pos induced 9145 neg induced         pos unin 9495       pos unin 9145
       0.2078             0.2296           0.1728              0.2175            0.2692
       0.2393             0.2589           0.2001              0.2561            0.2416
       0.1998             0.2197           0.1797              0.2289            0.2355

      0.2156             0.2361           0.1842              0.2342             0.2488



           0.1756             0.1961            0.1442              0.1942           0.2088




      33158.09452       29376.06256       268.1460934         77.25321888      78.23726649
SAFIRE II; Serial number: 807003897; Firmware: V 2.10 12/2007 Safire2; XFLUOR4SAFIREII Version: V 4.62n
Date:
Time:

Measurement mode:
Excitation wavelength:
Emission wavelength:
Excitation bandwidth:
Emission bandwidth:
Gain (Manual):
Number of reads:
FlashMode:
Integration time:
Lag time:
Plate definition file:
Z-Position (Manual):
Shake duration (Linear Medium):
Target Temperature:
Current Temperature:

Rawdata (RFU)
         <>                        1                     2                3                 4
         A                        47                    62               56               168
         B                        43                    55               42               102
         C                        43                    52               48               109
         D                        47                    44               25                46
         E                        43                    40               28                42
         F                        46                    41               26                42
         G                        26                    25               25                29
         H                        27                    26               27                28


{<cl02365 AtD>}          {<Pcryo_1225 AtD>}        {<ehaB>}        {<VtaA11 AtD>}    {<OprF AtD>}
          47                      62                    56               168              48
          43                      55                    42               102              47
          43                      52                    48               109              43
average
                    44                        56              49               126              46

subtracted by PBS
                  17                       29          22                       99             19
         2.309401077              5.131601439 7.023769169              36.25373544    2.645751311

  4500
  4000
  3500
  3000
  2500
  2000
  1500
  1000
   500
     0
          1    2    3   4   5   6    7   8   9 10 11 12 13 14 15 16 17 18 19 20 21
  -500




OD after 3 washes
{<cl02365 AtD>}         {<Pcryo_1225 AtD>}        {<ehaB>}       {<VtaA11 AtD>}    {<OprF AtD>}
        0.1915                 0.1981                0.2063           0.2068          0.1901
        0.1816                 0.1843                0.1846           0.1774          0.1914
        0.1553                 0.1529                0.1655           0.1626          0.1734
       average
        0.1761                  0.1784               0.1855          0.1823           0.1850

subtract PBS
background
               0.1361                    0.1384         0.1455            0.1423          0.1450



OD normalize
        127.3261508                 211.8950157 148.9459212          698.2193065    131.0646126


                                             OD normalized

  25000

  20000

  15000

  10000

   5000

      0
          1    2    3   4   5   6    7   8   9 10 11 12 13 14 15 16 17 18 19 20 21
OR4SAFIREII Version: V 4.62n
                     8/8/09
                      21:04

            Fluorescence
                         488    nm
                         575    nm
                           20   nm
                           20   nm
                           50
                           20
            High sensitivity
                           40   µs
                            0   µs
            COS96ft.pdf
                       5100     µm
                            5   s
                           37   °C
                           37   °C

            Temperature:                                                        37 °C
                  5                       6                7             8                 9              10
                 48                     119              128            60                28              54
                 47                      97              121            62                29              42
                 43                      99              110            64                28              46
                4129                   4272               46            38                35              28
                3882                   4281               44            38                39              27
                 26                      26               28            24                37              26
                 26                      28               26            27                25              27
                 27                      27               28            28                28              26


            {<Hag AtD>}         {<Hia AtD>}         {<upaG_short>} {<CPG_L6>}        {<eCPX>}        {<yuaQ AtD>}
                 119                  128                 60             28               54              45
                  97                  121                 62             29               42              48
                  99                  110                 64             28               46              45

                         105                  120              62               28              47             46


                        78                    93               35               1              20              19
               12.16552506           9.073771726                2     0.577350269     6.110100927     1.732050808

                                                      500


                                                      400


                                                      300


               Series1                                200
                                     100


                                        0
                                               1   2    3   4   5    6    7     8    9 10 11 12 13 14 15
                                    -100


                                  4, 6, 7, 8




{<Hag AtD>}      {<Hia AtD>}      {<upaG_short>} {<CPG_L6>}                   {<eCPX>}          {<yuaQ AtD>}
    0.1878           0.1830           0.1841         0.0389                       0.2106            0.2170
    0.1866           0.1746           0.1869         0.0382                       0.1972            0.2173
    0.1715           0.1570           0.1736         0.0551                       0.1851            0.1833

    0.1820           0.1715            0.1815               0.0441                  0.1976         0.2059



        0.1420           0.1315                0.1415               0.0041             0.1576          0.1659




  549.4247476      704.5108971        247.2915685                               128.9913301      114.5498392


                                                                                       OD normalized

                                    1000
                                     900
                                     800
                                     700
                                     600
  Series1                            500
                                     400
                                     300
                                     200
                                     100
                                       0
                                               1   2    3   4   5     6   7     8     9 10 11 12 13 14 15
       11                12
       45                43
       48                45
       45                41
       27                26
       26                26
       25                26
       26                28
       26                25


{<azo1653 AtD>}    {<CPG_L2>}        {<espP(beta)>}        {<TshA>}          {<AIDA-1 AtD>}
       43                47                  44                   25                46
       45                43                  40                   28                42
       41                46                  41                   26                42

              43                45                    42               26                     43


              16               18                    15                 -1                 16
               2      2.081665999           2.081665999        1.527525232        2.309401077




                              Series1
15 16 17 18 19 20 21




 {<azo1653 AtD>}    {<CPG_L2>}       {<espP(beta)>}          {<TshA>}           {<AIDA-1 AtD>}
      0.2067            0.1787             0.1717                 0.0362             0.1836
      0.1864            0.1704             0.1668                 0.0357             0.1773
      0.1831            0.1752             0.1696                 0.0371             0.1877

      0.1921            0.1748             0.1694                 0.0363             0.1829



           0.1521           0.1348                  0.1294            -0.0037             0.1429




     105.2170101       136.0375958          113.3728421                              114.3257116




                             Series1




15 16 17 18 19 20 21
pos induced 9495    pos induced 9145 neg induced        pos unin 9495        pos unin 9145
       4129                4272            46                  38                   35
       3882                4281            44                  38                   39
                                                                                    37

             4006               4277               45                   38                   37


             3979               4250            18                      11                   10
       174.655375        6.363961031   1.414213562                       0                    2
pos induced 9495    pos induced 9145 neg induced     pos unin 9495    pos unin 9145
      0.2305              0.2362         0.1802           0.2510           0.2579
      0.2329              0.2533         0.1505           0.2598           0.2643
                                                                           0.2026

           0.2317             0.2448        0.1654           0.2554            0.2416



           0.1917             0.2048        0.1254           0.2154            0.2016




      20753.78195        20754.57875   143.5979258      51.06778087       49.6031746
Measurement mode:
Excitation wavelength:
Emission wavelength:
Excitation bandwidth:
Emission bandwidth:
Gain (Manual):
Number of reads:
FlashMode:
Integration time:
Lag time:
Plate definition file:
Z-Position (Manual):
Shake duration (Linear Medium):
Target Temperature:
Current Temperature:

Rawdata (RFU)
      <>                   1             2            3                 4
       A                  69           479          2540             11607
       B                  56           369          1841              7462
       C                  58           348          1797              7056
       D                  29          6512           63                72
       E                  30          6649           60                41
       F                  28          6439           61                70
      G                   26            27           26                28
       H                  27            26           27                27

{<cl02365 AtD>} {<Pcryo_1225 AtD>} {<ehaB>} {<VtaA11 AtD>}      {<OprF AtD>}
       69               479           2540         11607               65
       56               369           1841          7462               54
       58               348           1797          7056               53
average
              61                399     2059               8708              57

subtract PBS
background
               33                371     2031              8680             29
                7        70.35860525 416.8505       2518.513517    6.658328118


  12000

  10000

   8000

   6000

   4000
   2000

         0
             1   2   3    4    5       6       7       8     9 10 11 12 13 14 15 16 17 18 19
  -2000



OD after 3rd wash
{<cl02365 AtD>} {<Pcryo_1225 AtD>} {<ehaB>} {<VtaA11 AtD>}           {<OprF AtD>}
      0.2858           0.2952        0.2522            0.1031            0.3195
      0.2871           0.2986        0.2719            0.0838            0.3296
      0.2501           0.2527        0.2267            0.1235            0.2832
average                                       pellet loss and filmy
           0.2743             0.2822   0.2503                 0.1035          0.3108


subtract PBS background
           0.2293                  0.2372                  0.2053           0.0585        0.2658



OD normalize
    143.8953488              1562.895292               9896.07         148466.3626   110.3725072
    143.8953488              1562.895292               9896.07                       110.3725072


                                           not accounting pellet loss

  160000
  140000
  120000
  100000
   80000
   60000
   40000
   20000
         0
             1   2   3   4    5    6       7       8       9 10 11 12 13 14 15 16 17 18 19 20 21




                                           not accounting pellet loss

  5000
4000

3000

2000

1000

   0
       1   2   3   4   5   6   7   8   9 10 11 12 13 14 15 16 17 18 19 20 21
Fluorescence
                     488   nm
                     575   nm
                      20   nm
                      20   nm
                      50
                      20
High sensitivity
                      40 µs
                       0 µs
COS96ft.pdf
                    5100   µm
                       5   s
                      37   °C
                      37   °C

Temperature:                                                                    37 °C
        5                           6                  7                   8                9
        65                       6828                4837                6058              62
        54                       4862                6147                5835              51
        53                       5132                7924                5265              46
       821                         86                 29                 3694              31
      1404                         98                 26                 3232              28
       700                        103                 27                 3677              27
        27                         28                 27                  29               27
        26                         28                 27                  28               28

{<Hag AtD>}                {<Hia AtD>}          {<upaG_short>}     {<CPG_L6>}        {<eCPX>}
       6828                       4837               6058                 29              6512
       4862                       6147               5835                 30              6649
       5132                       7924               5265                 28              6439

                    5607                 6303               5719                29               6533



               5579                    6275                5691                  1             6505
        1065.713532             1549.376111          408.957618                  1      106.6130073

                                                  1000
                                                   900
                                                   800
                                                   700
                                                   600
                                                   500
                   Series1                         400
                                                   300
                                                   200
                                                  200
                                                  100
                                                    0
19 20 21
                                                             1       2       3     4   5   6   7   8   9 10 11 12
                                                  -100

                                             2, 3, 4, 6, 7, 8, 10, 13, 16


  {<Hag AtD>}            {<Hia AtD>}          {<upaG_short>} {<CPG_L6>}           {<eCPX>}
           0.1559               0.1284               0.1719         0.0465               0.1443
           0.1350               0.1163               0.1928         0.0506               0.1497
           0.1097               0.1083               0.1758         0.0435               0.1488
  pellet loss and filmy pellet loss and filmy pellet loss and filmy               pellet loss and filmy
                  0.1335             0.1177               0.1802           0.0469              0.1476



                  0.0885            0.0727                   0.1352                                         0.1026




          63019.57831        86348.62385           42106.04192                                         63404.80832



                                                                             accounting pellet loss

                           25000

                           20000

                           15000
        Series1
                           10000

                            5000

                                0
21                                   1   2    3     4    5       6       7   8     9 10 11 12 13 14 15 16 17 18




                                                                                 accounting pellet loss

                           5000
               4000

               3000
     Series1
               2000

               1000

                  0
21                    1   2   3   4   5   6   7   8   9 10 11 12 13 14 15 16 17
      10                  11              12
     6156                 54              51
     4625                 50              50
     4688                 47              49
      28                  28              26
      27                  26              28
      27                  27              30
      28                  26              27
      30                  30              28

{<yuaQ AtD>}        {<azo1653 AtD>} {<CPG_L2>}           {<espP(beta)>}        {<TshA>}
      63                   72             821                    86                  29
      60                   41            1404                    98                  26
      61                   70             700                   103                  27

               61               61                 975                    96              27



            33                  33              947                    68                  -1
   1.527525232         17.34935157      376.4186499           8.736894948         1.527525232




                                                 Series1
           12 13 14 15 16 17 18 19 20 21




             {<yuaQ AtD>}   {<azo1653 AtD>} {<CPG_L2>}        {<espP(beta)>}    {<TshA>}
                 0.2436          0.2200          0.3523            0.2671            0.0432
                 0.2731          0.1331          0.6333            0.3035            0.0418
                 0.2769          0.1966          0.3280            0.3109            0.0412
loss and filmy
                     0.2645           0.1832           0.4379            0.2938           0.0421



                    0.2195          0.1382             0.3929            0.2488




              151.8372305      238.7267905       2410.487018       271.9356999
              151.8372305      238.7267905       2410.487018       271.9356999




                              Series1




        17 18 19 20 21
                 Series1




17 18 19 20 21
{<AIDA-1 AtD>}     pos induced 9495    neg induced 9495 {<cl02365 AtD>} unin
      3694                6156                 62                 54
      3232                4625                 51                 50
      3677                4688                 46                 47

            3534                5156                 53                        50



            3506                5128                 25                    22
     261.9662828         866.3096059        8.185352772           3.511884584
{<AIDA-1 AtD>}        pos induced 9495 neg induced 9495 {<cl02365 AtD>} unin
        0.0828               0.2844          0.3001             0.3065
        0.0814               0.2947          0.3377             0.3364
        0.0836               0.2789          0.3036             0.2877
pellet loss and filmy
             0.0826                0.2860         0.3138                0.3102



           0.0376             0.2410            0.2688                 0.2652




      93253.5461        21279.39142        93.00595238           84.21317245
                        21279.39142        93.00595238           84.21317245
{<Pcryo_1225 AtD>} unin        degunked {<CPG_L2>}
           51                           223
           50                           157
           49                           178

                          50                    186



                          22                    158
                           1             33.7194306
{<Pcryo_1225 AtD>} unin     degunked {<CPG_L2>}
         0.3498                    0.1044
         0.3509                    0.0900
         0.3056                    0.1042

                   0.3354                 0.0995



                   0.2904                 0.0545




             75.74888098             2897.310513
             75.74888098             2897.310513

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:4
posted:3/29/2012
language:
pages:54