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Gene Therapy Worksheet Apply your knowledge to convert the following description into a diagram that displays all of the information. One of the methods used for gene insertion in gene therapy involves using a virus. In order that the virus cannot replicate itself once inserted into the cell and cause damage to the host, the gene that codes for replication is removed from the virus. This is then replaced with a sequence of DNA that contains the normal allele for the desired protein that we want to introduce into the host. For example, in treating cystic fibrosis sufferers the DNA inserted would encode for the normal CFTR protein. Along with this gene, a promoter sequence of DNA which initiates transcription and translation of the inserted gene once inside the host, would also be inserted into the viral DNA. The virus is now ready to infect the host cells and introduce its DNA. Different types of viruses can be used – those whose DNA becomes part of the host DNA once infected, or those whose DNA remains independent. The latter type of virus is used when treating CF. Once the viral DNA is in the cell, the idea is that the normal process of protein synthesis occurs producing functional proteins from the DNA that has been inserted into the virus. Diagram Apply your knowledge to convert the following diagram into a paragraph or a written flow chart containing all of the details.
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