AP Biology Molecular Pre-Lab Worksheet by HC120324234143

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									AP Biology Molecular Pre-Lab Worksheet 4-13-10

http://www.phschool.com/science/biology_place/labbench/lab6/concepts1.html

   1. Explain when genetic transformation occurs.
   2. What gene will we use in this transformation lab?
   3. What will happen to the bacteria once the transformation has occurred?
   4. Which bacteria will we use in this lab? Where is it normally found?
   5. Give one of the main reasons E.coli is a good bacteria to use.
   6. E. coli has ______ chromosomes.
   7. Explain what a plasmid is.
   8. Explain how plasmids are useful in genetic engineering.
   9. Explain the difference between –ampR and +ampR.
   10. Do all plasmids have the ampR gene? Explain
   11. Explain how a –ampR bacteria can become +ampR.
   12. Explain what it means for bacterial cells to become competent.
   13. Explain how we will make these bacterial cells competent.
   14. Give the 4 stages of bacterial cell growth.
   15. What determines the length of each of these phases?
   16. Give the 5 requirements in order to maintain a relatively sterile environment in
       which to do this experiement.
   17. What will be the control group in this experiment?
   18. Explain how experimental plates 1 and 2 will differ from control plates 1 and 2.
   **Take the lab quiz until you understand what the results should be.

   Electrophoresis

   1. Explain the function of restriction enzymes and give another name for them.
   2. Explain what a recognition sequence is.
   3. How did the discovery of restriction enzymes make genetic engineering possible?
   4. What will we use electrophoresis for in this part of our lab?
   5. How will we calculate the size of the DNA fragments we create?
   6. Explain the idea of palindromic recognition sites.
   7. Explain what sticky ends are and how they are created.
   8. Why are they called sticky ends?
   9. Give a basic definition for recombinant DNA.
   10. Explain what a microliter is.
   11. What happens to a DNA sample that is mixed with one or 2 restriction enzymes?
   12. Explain how gel electrophoresis separates molecules.
   13. What affects the direction of movement of these molecules?
   14. What 4 factors affect the rate of movement of these molecules?
   15. DNA is a _____ charged molecule therefore it will move toward the _____ pole.
   16. Which DNA fragments will move the furthest and why?
   17. How is the length of each fragment measured?
   18. Which virul DNA will we use in this lab?
   19. Describe the structure of the 3 virul DNA samples we will be using.
20. What 3 things will we do with these 3 samples?
21. Which pole should the DNA wells be placed at? Why?
22. Explain why a tracking die is needed in this experiement.
23. Does the die actually stain the DNA? Explain
24. Why do we add sucrose or glycerol to our samples?
25. What will we use the micropipette for?
26. What will we stain the DNA with after electrophoresis?
27. Explain the purpose of the DNA marker and which sample acts as the marker in
    our experiment.
**Do the analysis of results II until you clearly understand it

								
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