Case It DNA Electrophoresis (PowerPoint)

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					 Case It! – A Project To Integrate Collaborative Case-based
Learning Into International Undergraduate Biology Curricula




           Mark Bergland, Karen Klyczek, Kim Mogen,
             Mary Lundeberg and Douglas Johnson

               University of Wisconsin-River Falls
 Contact information


URL for Case It! Home Page:
Http://www.uwrf.edu/caseit/caseit.html

E-mail address:
mark.s.bergland@uwrf.edu

Vol 5 of BioQUEST Library CD-ROM
             Case It! Overview


Electronic framework for analyzing and discussing
case studies in molecular biology
Human genetic diseases and associated ethical issues
Pre-existing and student-designed cases
Web-based “poster sessions”
Interactions among high school and university students
Pre-service science teachers as research assistants
                 Class-testing

 Students gather background information on cases using
  Case It! Investigator (v1.01) and web browser
 Analyze DNA sequences using Case It! Version 3.0/4.0
 Prepare web-page poster using automated system
 Poster session via Internet conferencing
 Role-playing (family members and genetics counselors)
 Mentoring of high school students by university students
    Techniques for DNA analysis

Features of Case It! Version 3.0
 DNA electrophoresis
 Restriction enzyme digestion and mapping
 Southern blotting

New features of Case It! Version 4.0
 Dot blotting
 Polymerase Chain Reaction
 More efficient file management via new floating windows
 Exports photos in GIF format rather than JPEG format – smaller files
  and no compression problems
 Makes possible more complex cases such as Jefferson-Hemings case
      Jefferson –Hemings Case


“In 1802, President Thomas Jefferson was accused of having
fathered a child, Tom, by Sally Hemings. Tom was said to
have been born in 1790, soon after Jefferson and Sally
Hemings returned from France. Present-day members of
the African-American Woodson family believe that Thomas
Jefferson was indeed the father of Thomas Woodson, whose
name comes from his later owner. No known documents
support this view.” (Foster et. al., Nature 396:27-28 (1998)
  Jefferson –Hemings Case


“Sally Hemings had at least 4 more children. Her last
son, Eston (born in 1808), is said to have borne a striking
resemblance to Thomas Jefferson, and entered white
society in Madison, Wisconsin, as Eston Hemings
Jefferson.”
    Jefferson –Hemings Case


“Although Eston’s descendants believe that Thomas
Jefferson was Eston’s father, most Jefferson scholars give
more credence to the oral tradition of the descendants of
Martha Jefferson Randolph, the president’s daughter.
They believe that Sally Hemings’ later children, including
Eston, were fathered by either Samuel or Peter Carr, sons
of Jefferson’s sister, which would explain their
resemblance to the president.”
Sample case: Huntington’s Disease


Susan is a 23-year-old whose father, age 55, and paternal
aunt, age 61, have been diagnosed with Huntington’s
chorea. A paternal uncle, age 66, appears to be unaffected
by the disease. Susan wants to know if she inherited the
mutated gene from her father so that she can prepare for
that future if necessary. She arranges to undergo DNA
testing for Huntington’s. Her 17-year old brother, John,
also decides to be tested after talking with Susan.
   Ethical issues raised by the case


Should someone with a family history of HD be required
to undergo testing? Should they have children if they test
positive, or if they have not been tested?

Should someone as young as John be tested?

Should the results of such tests be made available to
insurance companies? to potential employers? to potential
mates?
              Class testing

 Biology 100 - freshmen nonscience majors
 Groups choose a case, run the tests, interpret
  the results, and consider the ethical issues
  raised
 Play role of genetic counselors in session
  with “family members” (faculty)
 Poster presentations: live and via Internet
 Peer review
     Internet conferencing

 Student-generated web page posters accessed
  from Case It! home page
 Bulletin boards for peer review
 Comparisons:
  » Live versus web page poster sessions
  » Intra-school versus inter-school poster
    sessions
  » Intra-school versus inter-school
    collaborative teams
             Assessment

 Collaboration with College of Education
  (Mary Lundeberg, Co-PI)
 Confidence testing/gender issues
 Pre-service teachers conduct tests and analysis
 Videotape computer work, surveys, exit
  interviews, Internet poster discussions
                   Results

 Preservice teachers benefited from active
  participation in research project

 Case-based simulation enhanced interest,
  especially for female students

 Students, particularly females, became more
  confident in their knowledge
              Student quotes

"Case It! was definitely effective in my learning and
understanding of genetic testing. Not only effective, but
also interesting. Having a particular case and defect to
figure out drove the information home. Very good
program for college biology students. "


"I learned more [from this experience], than I have ever
learned in any science class! Plus, there was an actual end
result that we could be proud of. I definitely think this
helped and you should keep it going."
                 Student quotes

"By doing independent team research outside of class you
learn valuable research skills as well as interpersonal skills
when working with others. I enjoyed being able to learn (in
depth) about the disease. I was also glad we had a choice in
what disease we could pick. I think adding more diseases to
the Case It software would be good! I thought it was a non-
threatening way to learn and was fun."
"It seemed more real life than any other assignments, it
actually relates, it’s a real disease and we were using real life
like genetics and had real life like questions to answer.”

				
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