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GAGA-antibody-ab11962

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					Anti-GAGA antibody (ab11962)
   1 Image           URL for this product: http://www.abcam.com/GAGA-antibody-ab11962.html


 Product overview


Description                                Rabbit polyclonal to GAGA
Host species                               Rabbit
Tested applications                        WB
Cross reactivity                           Reacts with
                                           Fruit fly (Drosophila melanogaster)
Immunogen                                  This antibody was raised against a synthetic peptide corresponding to amino acid residue(s) 372-385 of Drosophila GAGA.
Positive control                           Schneider's Drosophila L2 nuclear extract can be used as a positive control for this antibody.


 Target


Relevance                                  The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of
                                           different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated
                                           genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ
                                           domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA
                                           protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo.
Cellular localization                      Nuclear
Alternative names
Alternative names                          GAF antibody
                                           GAGA factor antibody
                                           Nc70F antibody
                                           TFGAGA antibody
                                           Transcription factor GAGA antibody
                                           Trithorax like protein antibody


 Properties


Form                                       Liquid
Storage instructions                       Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer                             Preservative: 0.1% Sodium Azide, 0.01% Thimerosal (merthiolate)
                                           Constituents: 0.2% Gelatin, PBS
                                           See the website for more information on MSDS for this product.
Concentration                              0.05mg/ml
Purity                                     Protein A purified
Purification notes                         This antibody is affinity purified.
Clonality                                  Polyclonal
Isotype                                    IgG


 Applications


WB                                         There are no notes listed as specific to these applications. For more information please see the 'general application notes' section
                                           below or check the website.
General application notes                  WB: 1/500 - 1/1000. Detects a band of approximately 67 kDa (predicted molecular weight: 54.8 kDa). For optimal results, primary


                                                                                                                                                             Last updated on
UK office:                                      US office:                                                                                                   February 28, 2012
Abcam plc                                       Abcam Inc.
330 Cambridge Science Park Cambridge CB4 0FL UK 1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA
 el:
T +44 (0)1223 696000                             el:
                                                T 617 225 2272
Fax: +44 (0)1223 771600                         Fax: (617) 849-5829
Email: orders@abcam.com                         Email: us.orders@abcam.com
                                            antibody incubations should be performed at 37°C.

                                            Not tested in other applications.
                                            Optimal dilutions/concentrations should be determined by the end user.


 Images (See the website for higher resolution images of this product)



                        Predicted band size : 54.8 kDa


                        ab11962 at 1/500 (Lane 1), 1/1000 (Lane 2), 1/2000 (Lane 3) staining Drosophila GAGA in Schneider's Drosophila L2 nuclear extract by Western blot
                        (ECL). Lane 4 shows elimination of GAGA signal by addition of immunising peptide to the reaction mixture.




Our Abpromise to you: Quality guaranteed and expert technical support

If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit
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                                                                                                                                                         Last updated on
UK office:                                      US office:                                                                                               February 28, 2012
Abcam plc                                       Abcam Inc.
330 Cambridge Science Park Cambridge CB4 0FL UK 1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA
 el:
T +44 (0)1223 696000                             el:
                                                T 617 225 2272
Fax: +44 (0)1223 771600                         Fax: (617) 849-5829
Email: orders@abcam.com                         Email: us.orders@abcam.com

				
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