Basics of Chromatography by l62idC8

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									   Basics of Chromatography
• Separation of Analytes
• Type of Equilibrium

                 CS
             K 
                 CM
Basics of Chromatography
            • Ability to dissolve in
              mobile phase
            • Interaction with
              stationary phase
            • Time on X axis
            • Response on Y
• Chromatography Types
• Separation dependent
  on time on the column
  (along with other
  factors)
• Retention time of the analyte
• Retention time of the Mobile Phase
   Basics of Chromatography
• Average linear flow rate
• Average linear flow of mobile phase

        L                    L
                       
        tR                  tM
• Plate Height
• Theoretical Measure
  of Efficiency



                  2
      H 
                L
• Fig. 26-6
• Determining standard deviation from a peak
• Std dev = τ    W=4τ
    Basics of Chromatography
• Capacity Factor
• Selectivity Factor
                           tR  tM
  k 
   '  K AVS            k 
                        '

       VM                     tM

        k   '
                          (t R ) B  tM
          B          
        k   '             (t R ) A  tM
            A
   Basics of Chromatography

• The Van Deemter Equation



        H = A + B/u + Cu
• Multiple Flow Paths
• Tortuosity
• Components
  of the
  VanDeemter
• Van Deemter Plot
• How Plate Height is effected by particle size in
  solid packing
   Basics of Chromatography
• Column Efficiency


       L                       tR 2
                      N  5.54( )
    N                         W1
       H                        2


                   tR 2
               N ( )
                   W
Resolution
   Basics of Chromatography
• Resolution


            (t R ) B  (t R ) A
       RS 
                    W
The Effect of Mobile Phase on Retention
More on Resolution

								
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