Granules by f2968A

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									                                Leukocyte Development
                                           CLS 312 Hematology




General Statements: With Maturation-

    Cell Size:                       progressive decrease
    Cytoplasm Color:                 lightens
    Nuclear Size:                    progressive decrease
    Nuclear Color:                   purple/red--->dk blue
    Nuclear Chromatin:               fine/delicate--->clumped
    Granules:                        none-->azure-->specific

Myeloblast: 3-5 nucleoli usually visible

    most immature form
    dark blue cytoplasm, but not as dark as rubriblast
    15-20 micron diameter
    large nucleus relative to cytoplasm
    cytoplasm nongranular
    nucleus round, without folds or lobules
    chromatin fine, delicate and purple red in color

Promyelocyte: also called progranulocyte

    cytoplasm dark to pale blue
    appearance of non-specific, azurophilic, primary granules appearing first at the periphery of the Golgi
    Zone.
    nuclear outline round to oval
    centrally located OR eccentric nucleus
    nuclear chromatin stranded
    parachromatin space quite distinct
    nucleoli may be visible

Myelocyte: cytoplasm shows appearance of specific granulation
  may have some azurophil granules left over
  cytoplasm rather light blue
  presence of identifiable specific granules is key to identification
  round or oval, eccentric nucleus
  some chromatin clumping and coarse strands
  nucleoli may be present, but not usually distinct
Metamyelocyte: not normally seen, except in Bone Marrow.
   diffusely blue-gray or pinkish cytoplasm
   scattered azurophil granules
   many specific granules (secondary granules)
   usually kidney-shaped nucleus, but may be only slightly dented
   chromatin clumped into ropy strands

Band Form: also called Stab

Committee for the Clarification of the Nomenclature of Cells and Diseases of the Blood and Blood
Forming Organs standard definition for a band is as follows:

    "Any mature cell of the granulocytic series which has a curved, band shaped nucleus, but which has
    not developed a thread like filament, shall be called a band or stab. If any nuclear chromatin-
    parachromatin space is seen in the bridge between the two lobes then the bridge is not a filament and
    the cell is a band. If the two sides of the nuclear bridge are parallel for an appreciable distance, then
    the cell is a band, not a metamyelocyte.
    Any cell in which the nucleus is twisted in such a fashion in which the entire outline of the nucleus is
    not visible, due to superimposition of one part of the nucleus on other parts, shall be classified as a
    poly."

Polymorphonuclear Form:                also called "poly" or "seg"
    segmented, lobulated nucleus
    specific granulation
    neutrophilic, basophilic, or eosinophilic granulation


LEUKDEVP.DOC
Tuesday, February 21, 2012

								
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