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Stat 415_ section 3. HW 1 solutions This is written in Word_ which

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					Stat 415, section 3. HW 1 solutions

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1) Echinacea metabolites
a) Pur504 (row 30)

You are looking for the sample with the smallest distance. That’s most easily done by looking at the Pur307 row of the
matrix containing the distances.

lipo.m <- as.matrix(lipo[,-(1:2)])
dimnames(lipo.m)[[1]] <- lipo$access

lipo.canb <- vegdist(lipo.m, ‘canb’)
temp <- as.matrix(lipo.canb)
temp[‘Pur307’, ]

The smallest is Pur504 (row 30) with a distance of 0.426

b) There are number of metabolites that are in Pur307 and Pur504, but not in Ang267.

lipo.m[c(1,28, 30),]

c) Pur 313

lipo.pa <- decostand(lipo.m, 'pa')
lipo.jd <- vegdist(lipo.pa, 'jacc')
temp <- as.matrix(lipo.jd)
temp[‘Pur307’, ]

The smallest is Pur313 (row 29).

d) Most metabolites are present in all 3 samples or absent in all three samples. There are two that are not. Pur307 and
313 differ in metabolite (unknown_A2). Pur307 and 504 differ in 2 (unknown_A2 unknown_B7).

lipo.pa[28:30,]

e) I expect them to be quite similar because the two distance measures are highly correlated.
The plot is on the next page
           0.8
           0.6
 lipo.jd

           0.4
           0.2
           0.0




                 0.2        0.4             0.6        0.8          1.0

                                     lipo.canb

f) 3 Dimensions. Stress for the nMDS 2D solution is 11.5%. That for the 3D solution is 7.9%

lipo.mds2 <- metaMDS(lipo.canb, k = 2, expand=F)
# because we’re starting with the distance matrix autotransform=F is not necessary
#or, to get the species scores, use the data matrix
lipo.mds2 <- metaMDS(lipo.m, ‘canb’, k=2, autotransform=F, expand=F)

lipo.mds2 <- metaMDS(lipo.canb, k = 2, expand=F)

g) The very obvious ones are Pur, Lae, Tenn. All three Pur samples and all Tenn samples are on the edge of the plot of
samples, close to each other and relatively well separated from the rest. The three Lae samples are also close to each
other in the middle of the plot but separated from the other species.

ordirgl(lipo.mds3)

Note: Pal215 is one sample that stands out from the rest, but the other Pal samples are quite distant.

h) Again, Lae, Pur, and Tenn are the species relatively well separated from the rest (plot on next page).

ordiplot(lipo.mds2,type=’t’,disp=’sites’)
         0.4

                             Tenn326
                             Tenn325
                      Tenn324
                          Tenn250
         0.2




                AngAng318

                   Sang258                                                          ParaNeg264
                     Sang878                                                          Hyb306
                    Sang257                                                          ParaNeg265
                                                                                   ParaNeg263
                                                                                 Pal293
                                                                               Simu308
 NMDS2




                Pal275                                                               Simu304
         0.0




                 AngAng285                            Lae310
                                                     Lae314                       AngStr320
                                                                              Pal290
                  AngStr266
                       AngAng272                                                    ParaPara301
                                                                                Pal296
                                                     Lae312                         ParaPara321
                                                      Lae316       Simu249           Hyb294
                       Atr260                                                  ParaPara292
                  Atr262
                  Atr255 Ang267
                    Atr299
         -0.2




                    Pal215


                                          Pur504
         -0.4




                               Pur307
                                           Pur313



                    -0.4           -0.2             0.0         0.2           0.4

                                                    NMDS1


i) Your explanation here is more important than your specific answer.
I would use the 2D plot because it represents the same features seen in the 3D plot, even though the stress is slightly
above 10%.

j) Unknown B1, Unknown B6, amide 16, amide 13.

Note: You are looking for the compounds (species scores to vegan) that are plotted in the direction of the Tenn samples.
To get the species scores, you need to do the mds on the raw data matrix.

ordipointlabel(lipo.mds2)
k) Yes, the plot does.

                                                                  lipo.m[, "amide_16"]



                       1.0
                                                           0.07

                                                    0.06
                                                              0.05
                       0.5

                                                       0.04

                                           0.03

                                                           0.02
              NMDS2

                       0.0




                                               0.01
                       -0.5




                                           0
                       -1.0




                              -2               -1                          0             1      2

                                                                          NMDS1

amide 16 increases towards the locations of the Tenn samples.

ordisurf(lipo.mds2, lipo.m[,'amide_16'])

l) Yes, the arrow appropriate summarizes the variation in amide 16. The arrow points in the direction of increasing
amounts of amide 16, the contours from ordisurf are reasonably parallel and equi-distant.

                                                                  lipo.m[, "amide_16"]
                      1.0




                                                           0.07
                                                                      2
                                                                      1
                                                    0.06
                                                              0.05
                      0.5




                                                      0.04

                                           0.03

                                                           0.02
            NMDS2

                      0.0




                                               0.01
                      -0.5




                                           0
                      -1.0




                              -2               -1                          0             1       2

                                                                          NMDS1
2) Missouri River fish data
a) Bray-Curtis after standardizing to site total and Morisita-Horn are very highly correlated. Jaccard is much less
correlated. BC and MH will give similar results; Jaccard may give very different results.

Note: the curvature in BC vs MH plot is not relevant (at least for nMDS) because nMDS is based on ranks, not linear
correlation.

fish.bray <- vegdist(decostand(fish.m,’total’), ‘bray’)
fish.mh <- vegdist(fish.m, ‘horn’)
fish.j <- vegdist(decostand(fish.m, ‘pa’), ‘jacc’)
pairs(cbind(fish.bray, fish.mh, fish.j))



                                                               0.0   0.2    0.4   0.6   0.8   1.0




                                                                                                                                        1.0
                                                                                                                                        0.8
                                                                                                                                        0.6
                                      fish.bray




                                                                                                                                        0.4
                                                                                                                                        0.2
      0.0 0.2 0.4 0.6 0.8 1.0




                                                                           fish.mh


                                                                                                                                        0.0 0.2 0.4 0.6 0.8 1.0




                                                                                                                fish.j



                                0.2    0.4   0.6   0.8   1.0                                        0.0   0.2   0.4   0.6   0.8   1.0



b) Yes. Standardizing by site total removes the dependence on total abundance. BC is most sensitive to differences in
the abundant species. MH would have also been appropriate.

c) You would need 4 dimensions if you strictly demanded stress < 10%.
Stress for a 2D solution is 0.143, for a 3D solution is 0.102, for a 4D solution is ca 0.080.

fish.mds2 <- metaMDS(fish.bray, k=2)
fish.mds3 <- metaMDS(fish.bray, k=3, trymax=200)
fish.mds4 <- metaMDS(fish.bray, k=4, trymax=200)
Note: it was hard to find the best 3D solution and even harder to find the best 4D.
trymax= tells metaMDS to try even more starting positions.

d) gear is the only factor with visually obvious association with species composition.

The convex hulls for sites and habitats overlap each other, but those for some gears don’t overlap.

plot(fish.mds2,disp='sites')
ordihull(fish.mds2,fish.env$site,label=T)
title("Sites")

plot(fish.mds2,disp='sites')
ordihull(fish.mds2,fish.env$habitat,label=T)
title("Habitats")

plot(fish.mds2,disp='sites')
ordihull(fish.mds2,fish.env$gear,label=T)
title("Gears")


                                                    Sites
         0.2




                                                      C
         0.0




                                                    L
 NMDS2




                                                  O
         -0.2
         -0.4




                    -0.4           -0.2            0.0            0.2            0.4          0.6

                                                   NMDS1
                                     Habitats
         0.2




                                      m
         0.0
NMDS2




                                       c
         -0.2
         -0.4




                 -0.4        -0.2    0.0        0.2          0.4     0.6

                                     NMDS1



                                       Gears
          0.2




                        SH

                                                        FN    SN
          0.0
 NMDS2




                             LH
          -0.2




                                       EF
          -0.4




                  -0.4        -0.2     0.0        0.2          0.4     0.6

                                       NMDS1
e) OcLH02 is very different from the others. The two species strongly associated with that sample are GZSD and BKCP.

Note: You are looking at for a point well separated from the others. The species most strongly associated with that
sample are those with species scores closest to OcLH02.

f) When this HW was assigned, we knew about two ways to answer this question: visual assessment, e.g. using convex
hulls and testing association between the ordination and the habitat using envfit. Either was appropriate.

In neither approach is there evidence of a difference.
Visually:


                                    Habitats, LH gear
         0.0 0.2 0.4




                                  mc
 NMDS2

         -0.4
         -0.8




                       -0.5      0.0            0.5             1.0            1.5

                                            NMDS1


fish.lhmds <- metaMDS(decostand(fish.lh, ‘total’), autotransform=F, expand=F)
plot(fish.lhmds, disp=’sites’)
ordihull(fish.lhmds, fish.envlh$habitat)

The envfit test has a p-value of 0.97. (because this is permutation based, you may have a slightly different p-value).
No evidence of a difference among habitats.

envfit(fish.lhmds, fish.envlh$habitat)

				
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