B cell Maturation and
Activation
Chapter 11
Affinity maturation
of the antibody
response
B cell differentiation in the germinal center
mature
B cell
bacteria helper
T cell
Centroblast
DARK ZONE -CLASS SWITCH
RECOMBINATION
LIGHT
ZONE -SOMATIC
HYPERMUTATION
FDC
centrocyte
Memory apoptosis
B cell
anergic
Plasma B cell
Somatic hypermutation introduces base changes
in the antibody V region
Point mutations occur at high rates of ~10-3 mut/bp/gen in vivo
AID Induces Somatic
HyperMutation
Martin et al, 2002
Mutations in variable-region mRNA
Proposed enzymatic activity for AID
dC to dU deamination in DNA
B cell differentiation in the germinal center
mature
B cell
bacteria helper
T cell
Centroblast
DARK ZONE -CLASS SWITCH
RECOMBINATION
LIGHT
ZONE -SOMATIC
HYPERMUTATION
FDC
centrocyte
Memory apoptosis
B cell
anergic
Plasma B cell
Role of AID in B cell Repertoire
Development
Kenter, 2002