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Medicinal herbs for peptic Ulcer Areta lanata pindi konda

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Medicinal herbs for peptic Ulcer Areta lanata pindi konda Powered By Docstoc
					Introduction:

Some dates back to centuries, plants have been used to treat various diseases and their importance is
considerable now a days. Therefore the use of medicinal plants all over the world has gained great
importance. So it is necessary to study the effect of these plants on various diseases. Peptic ulcer is a
patho physiological condition where the mucosal layer is eroded characterized by inflamed lesions, also
known as ulcer peptium, peptic ulcer disease (PUD). Peptic ulcer in stomach is called as gastric ulcer. It is
most common chronic disorder in duodenum and became a global health problem effecting larger
number of people through the world mainly in India. Aerva lanata is a small woody prostate or succulent
perennial herb, commonly known as pindi konda (Telugu), Gorak butti (Hindi), Sirru-pulay-vayr (Tamil).
The herb grows wildly everywhere on plains of India. It is a common leafy vegetable and its extract is
used traditionally on fresh wounds, lithiasis , gonorrhea etc., scientific works has been done to prove
this plant as diuretic, anti inflammatory anti tumor, anti microbial, anti malarial, anti venin, sedative and
demulcents. But no work has been reported on anti ulcer activity of leaf extract of Aerva lanata. Keeping
this in view the present study has been under taken to investigate the anti ulcer activity of Ethanolic leaf
extract of Aerva lanata, Linn (ELEAL) in experimental l animal models.

Materials and methods:

Collection of plant material:

The fresh green leaves of plant Aerva lanata were collected during the month of August from wild
source. And the plant material was taxonomically identified by Mr.________, lecturer in botany in AKRG
Degree College. About 1 kg Leaves were washed with water free from the dust and shade dried. They
were packed in air tight container.

Preparation of extract:

Under the procedure of cold maceration the dried leaves were grounded to fine powder and soaked in
ethanol for n7 days. After 7 days, the solution was filtered and mark was pressed. The obtained was
made concentrate by distillation followed by evaporation.

Animals:

Swiss albino rats of wistar strain of both sex weighing 120-130 gm were selected for the study from the
animal house of AKRG college of pharmacy. They were maintained under std. environmental conditions
and fed with std. diet provided in animal housing.

Materials:

     Gum acacia – vehicle for all preparations

     Omeprazole – standard drug
Acute Oral toxicity studies:

The study of acute oral toxicity was conducted in accordance with CPCSEA guide lines. The animals were
acclimatized under lab conditions before study and mortality was seen up to 2000 mg/kg in the study.
1/10 th and 1/5 th of upper dose limit are selected as test I and test II respectively for the experiment.
Following regimen of treatment was given for each group animals per orally.

Experimental Design:

The selected animals were divided into 4 groups as 4 animals of both sex in each group. Different
markings were given to all animals to avoid mingling.

     GROUP I – control receives (3% gum acacia)

     GROUP II – Test I receives ELEAL 200mg/kg

     GROUP III – Test II receives ELEAL 400mg/kg

     GROUP IV – Standard receives Omeprazole 2mg/kg

METHODOLOGY:

 Animals were fasted for 24 hours before the study but had free access to water. Animals in group I
received only distilled water. Group II and Group II received 200mg/kg and 400mg/ kg of ELREAL
respectively. Animals of group IV received standard drug. Coprophagy was avoided during fasting.

Gastric ulcers induced by pylorus ligation: For The 48 hrs fasted animals, at 50 th hour administer the
drug to be given. After 3 hours of the drug administration, under light ether anesthesia, incision was
made at xyphoid process to open abdomen and pylorus was ligated without causing any damage to its
blood vessels. The stomach was replaced carefully and abdomen was closed with interrupted sutures.
The animals were deprived of water during post operative period. After 4 hr s from ligation the animals
were sacrificed either by high anaesthesia or decapitation. Stomachs were dissected out and the
contents of stomach were collected carefully into cleaned test tubes. After recording the ph of the
gastric content, they were centrifuged, filtered taken to determine the free acidity and total acidity
according to the standard procedures. The stomach was cut along the greater curvature, stretched on a
labeled piece of plank on black background. Observed for ulcers and ulcer index was calculate. Along
with various parameters studied above, some histopathological parameter were studied and compared
among all four groups of animals.

Statistical analysis:

It was carried out using ANOVA , followed by Dunnet’s multiple comparison tests using graph pad PRISM
software P values<_________ were considered to be significant.

Results and Discussion:

				
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posted:2/13/2012
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Description: Articcle from my associates on Peptic Ulcer research carried out on Areta lanata