Appendix Rodent Breeding

							                             SOP, Rodent Breeding Appendix
                                      Appendix 1

1. Please justify the need to establish and maintain a breeding colony. Are these animals not available
   from commercial vendors?

2. State source of founder animals. State number of founder animals required to initate this colony.
   Confirm that all animals brought to UMB from any source will be coordinated through Veterinary
   Resources to assure that proper health records are obtained, reviewed and approved before animal
   shipment.

3. Animal Numbers - Adult Breeders:
      a. State the number of breeders per year necessary to conduct this work.
      b. State the age criteria for the gradual replacement of breeder.
      c. State the fate of retired breeders.

4. Animal Numbers - Offspring Generated:
    Please complete the following table. Additional rows can be added to this table by placing the cursor at the far
    right of the last row and hitting enter.

         Breeders        # of breeding     Offspring: strain   # Offspring used    # Offspring deemed    # Offspring to
      Strain x strain        pairs           designation       experimentally **        unusable        replace breeders




**This number should match the numberof animals of this strain requested in the experimental protocol.

5. Describe the breeding scheme used to establish and maintain each genetic line,
   homozygote/heterozygote, inbreeding, out crossing, hybrids, background strains, etc.        Please
   indicate at what age animals will be weaned. The standard is 21 days. If animals must be weaned
   at a later point, please specify at what age they will be weaned and provide justification for that
   request. Please contact Veterinary Resources for assistance as needed.

6. Discuss how genetic integrity will be maintained.

7. Describe procedures for genotyping/phenotyping or other testing methods to be performed?
        Please review the tail docking guidelines to insure that the described procedures are consistent with the
        most recent guidance document.

8. Discuss whether the generated mice experience any significant health problems associated with their
   genetic status, e.g. immunodeficiency, muscloskeletal deficiencies, any problems associated with
   eating, drinking, metabolism which requires special needs or results in pain or distress. Please
   discuss the severity of symptoms expected in these animals, if any. What measures will be used to
   assess the symptoms and relief from pain and/or distress, if necessary. Please assure that the IACUC
   that all moribund animals will be euthanized.

9. Please indicate the expected life span for each strain of mouse to be bred.




                                                                              IACUC, School of Pharmacy, 01/24/2008
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                           SOP, Rodent Breeding Appendix
                                    Appendix 1

10. Identify the individual(s) responsible for maintaining the colony and for keeping all breeding and
    testing records. Please indicate each person’s experience and training with this species and with
    managing/maintaining a breeding colony.

11. What nomenclature is used for the strains being bred?

12. The NIH Guidelines for Research Involving Recombinant DNA Molecules requires that the
    Institutional Biosafety Committee review and approve experiments involving the generation of
    rodents in which the animal’s genome has been altered by stable introduction of recombinant DNA,
    or DNA derived therefrom, into the germ-line (transgenic animal).

    Are two transgenic (or knockout) mouse strains being bred to create a new mouse strain?
                ____YES        _____NO

        Is a transgenic (or knockout) mouse strain being bred to a new background strain?
               ____YES       _____NO

        If yes, the following questions must be answered:
        a. Describe the unique characteristics of the the transgenic founder(s).
        b. Describe the expected unique characteristics of viable offspring.
        c. Indicate the type of confinement used to house these animals

        ___ ABSL1      ___ ABSL2       ___ ABSL3

        d. Describe the precautions that will be taken (or procedures used) to minimize the possibility
           that animals could escape confinement.
        e. Assume that progeny of the breeding pairs were to escape and mate with wild-type animals.
           Describe the potential consequences of this event upon the wild population of animals.




                                                                       IACUC, School of Pharmacy, 01/24/2008
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                              SOP, Rodent Breeding Appendix
                                       Appendix 1

SAMPLE Flow Charts #1:

One Breeding Pair

Male    +         Female




       __%                 __%
       Genotype            Genotype


___% -- used in experiments; ___% -- unusable as result of genotype, sex, etc


SAMPLE Flow Chart # 2:

One Breeding Pair

Male    +         Female




        _#_ pups / litter

        _#_ litters / year

        _#_ pups / year / breeding pair + 2 breeders X ( _#_ breeding pairs/year) = _#_ per year




                                                                      IACUC, School of Pharmacy, 01/24/2008
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