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                                LSSG-CT-2004-013032
                                          ELIfe
                 European Lipidomics Initiative: Shaping the Life Sciences

Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6




       TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

                      Publishable Final Activity Report



                                                      Final Report
                                                       2005-2007




Period covered: from 1/1/06 to 1/7/07                                          Date of preparation: 1/1/08
Start date of project: 1/1/05                                                  Duration: 2.5 years
Project coordinator name:                                       Prof. Gerrit van Meer
Project coordinator organisation name:                          Utrecht University
                TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

                            Publishable Final Activity Report




Contents                                                        page

1. Project execution                                             3

       Project Objectives                                        4

       Contractors Involved (for the duration of the project)    5

       Work Performed and End Results                            6

2. Dissemination and use                                         8

       References                                                9


Annex 1 - Survey of expertise and infrastructure within the
                     Lipidomics field

Annex 2 - Plan for Using and Disseminating Knowledge
             (extended version)

Annex 3 - Plan for Using and Disseminating Knowledge
             (publishable version)

Annex 4 - Final management report (pending)

Annex 5 - Final report on the distribution of the Community's
                     contribution (pending)

Annex 6 - Questionnaires




                                                                       2
                  TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

1. Project execution

The research areas that deal with the major chemical constituents that build up the cells in our
body are referred to as genomics for genes and nucleic acids, proteomics for proteins and
glycomics for carbohydrates. What has been missing in the “OMICS” realm is lipidOMICS.
Since both carbohydrates and lipids are cellular metabolites, glycomics and lipidomics are
subdivisions of metabolomics.

The time has come for generating broad insights into the role of lipids in physiology and
pathology. Given that thousands of different lipids are present within a single cell and that
many of these lipids are involved in modulating the processes of life in an area that is
upcoming, lipidomics describes and quantitatively analyses the full complement of lipids, in
for example body fluids, cells and tissues. Lipidomics integrates these data with knowledge of
their protein targets, i.e. the metabolic enzymes and transporters, and of the relevant genes
and the regulatory aspects of these physiological systems. Above all, an understanding of cell
membranes will not be possible without understanding their lipid constituents.

Most important is the fact that many of the widespread diseases that plague humankind
involve lipids. Prime examples are cardiovascular disease, obesity-related type-2 diabetes, and
stroke. Other major diseases such as cancer and Alzheimer's disease also have a lipid
involvement. In addition to these disorders of epidemic proportions, there are many other
diseases that are directly caused by inherited defects in lipid metabolic enzymes and
transporters, such as defects in cholesterol synthesis and lipid storage diseases. Lipids also
play major roles in autoimmune diseases and act as (co-)receptors for bacteria, viruses and
toxins. An increase in our knowledge of disease-related changes in lipid patterns and its
integration into proteomic and genomic data will provide new basic biomedical insights; thus,
far-reaching possibilities for diagnostic application (prognostic assessment, diagnosis and
monitoring) as well as for the development of prevention and new therapeutic approaches can
be expected.

Although lipid research was well developed in the initial era of biochemistry in the 1960s and
70s, it lagged behind the more recent developments of genomics and proteomics. One major
problem has been the lack of technology to analyze the thousands of different lipids in body
fluids and cellular systems. However, this problem is now quickly being overcome by
exquisitely sensitive high-throughput mass spectrometric methods that are revolutionizing the
field. Still, the application of such techniques to solving basic biomedical problems has
remained rare and the clinical use of the potential of lipidomics is sparse. Finally, at the start
of the present initiative there were no fora where basic biomedical scientists and clinicians
would meet the technology developers, bioinformaticians and industrial stakeholders who can
provide the infrastructure and the standardized kits needed for clinical testing.




                                                                                                 3
Project Objectives

The primary aim of the European Lipidomics Initiative (ELIfe) was to mobilize and organize
key stakeholders, researchers and end-users in the area of metabolomics, especially lipidomics
research, and to further define this field of research in terms of participants, scientific contents
and strengths.

Objective 1: To network the field of metabolomics and to seek alliances with relevant
stakeholders
The consortium should network and position the field of Lipidomics. Other areas of
Metabolomics research like Glycomics and Signalomics (signaling lipids, lipid hormones)
would be involved in all activities. Researchers, stakeholders from the health care profession
and from industry with special emphasis on SMEs would be invited to join ELIfe activities at
the earliest possible stage.

Objective 2: To link the field of metabolomics to the genomics and proteomics initiatives
One special challenge was to adapt bio-informatics as devised for Genomics and Proteomics
to the field of Metabolomics. The choice of the technology to be used would define the type
of bio-informatics approaches necessary to deal with the large body of data expected from
Lipidomics and in a wider sense Metabolomics.

Objective 3: To define a strategy for metabolomics research, using lipidomics as example
Lipidomics was subdivided into three areas of development: i) technology, ii) cell biology,
and iii) health. Experts in these areas were to be joined in a series of workshops to define
where these areas would gain most from a European collaboration and what direction of
research would most likely bring breakthroughs. The topic of lipid rafts would be taken as a
theoretical test case to define the kinds of experimental data that can be generated by high-
throughput approaches in this field, and to determine how these data sets could be most useful
for medical application.

Objective 4: To initiate an Expertise Platform on Lipidomics
As a first step to fully mobilize the field, an awareness of the strength and expertise was to be
created. Via a survey of expertise, to be published on the web and via a specific workshop on
Lipidomics and Technology, a virtual Expertise Platform would be initiated and linked to the
Euro Fed Lipids organization. It was to become a test centre for bench marking of new
Lipidomics technology.

Objective 5: To hold both science-related as well as policy meetings
A series of scientific and strategic meetings had to be organised. (a) Workshops with a focus
on technology and terminology, on basic science and on medical applications of
metabolomics and of lipidomics in particular. (b) Networking meetings and applications
meetings should join academic scientists across the life sciences, clinical laboratory scientists,
clinicians and industrial representatives. (c) The concluding conference should be open to
policy makers as well as the above mentioned representatives, and specific round tables were
to be organized for this purpose.




                                                                                                  4
Contractors Involved (for the duration of the project)

     Partic.    Participant name   Participant                         Address             Country
      No.                          short name
       1        Gerrit van Meer    UU-CBLE       Utrecht University                          NL
       2        Gerd Schmitz       UniReg        University Hospital Regensburg              D
       3        Kai Simons         MPI-CBG       MPI-CBG, Dresden                            D
       4        Jürgen Borlak      Fh-ITEM       ITEM, Hannover                              D
       5        Raymond Dwek       OGBI          Oxford University                           UK
       6        Pam Fredman        UGOT          Göteborg University                         S
       7        Felix Goñi         UPV-EHU       University of the Basque Country, Leioa     E
       8        Elina Ikonen       UniHsk        University of Helsinki                     FIN
       9        Michel Lagarde     INSERM        INSA Lyon                                   F
       10       Konrad Sandhoff    KI            University of Bonn                          D
       11       Balázs Sarkadi     NMC           National Medical Center, Budapest           HU
       12       Fritz Spener       UniGraz       University of Graz                          A
                Sepp Kohlwein
       13       Sandro Sonnino     UniMi         University of Milano                         I
       14       Gerd Utermann      IMBHG         Medical University Innsbruck                A

Co-ordinator:        Prof.dr. Gerrit van Meer
                     Bijvoet Center             T:       +31-30-253.3427
                     Utrecht University         E:       g.vanmeer@uu.nl
                     Padualaan 8
                     3453 CH Utrecht
Project manager:     Dr. Bas R. Leeflang
                     Bijvoet Center             T:       +31-30-253.3498
                     Utrecht University         E:       b.r.leeflang@uu.nl
                     Padualaan 8
                     3453 CH Utrecht
Project website: www.lipidomics.net
Lipidomics Expretise Platform: www.lipidomics-expertise.de




                                                                                                     5
Work Performed and End Results

Expertise Platform and the inventory of stakeholders (Objectives 1 and 4).
The Lipidomics Expertise Platform was launched on the internet in November 2005. The
database was used to prepare a survey of the lipidomics expertise and infrastructure in
Europe. A database containing 2,500 active e-mail addresses was used to mobilize
lipidologists for the concluding ELIfe meeting. Scientists from industry were specifically
invited for the first workshop (Dresden, 2005) focusing on lipidomics technology, which led
to the exchange of ideas on technological possibilities versus the needs of the scientists in the
field.

Contacts were established between ELIfe and related initiatives in the US (LipidMAPS) and
Japan (LipidBank). As a first collaborative step, a common lipid classification scheme has
been devised and published [1-3]. In this context, professors Spener and van Meer were
invited to become members of the International Lipid Classification and Nomenclature
Committee (ILCNC), which has taken on the task of updating and expanding the
classification system. The committee concluded its first meeting on July 7, 2006, with a list of
recommendations in the light of a continuously evolving inventory of biological lipid
structures.

Lipidomics versus genomics/proteomics and the need for bioinformatics (Objective 2).
Genomics, proteomics and metabolomics experts were invited to present their points of view
at the ELIfe kick-off meeting. The recognized need for specific bioinformatics approaches
was then addressed by inviting the lipid MAPS experts for the first technology workshop in
Dresden, and subsequently by active participation of ELIfe members (with LipidMAPS
representatives) in an extra workshop at the European Bioinformatics Institute in Hinxton that
concentrated on connectivity between different types of databases, the willingness of EBI to
provide the physical storage space for lipid databases, and the curation of existing databases.
This resulted in the involvement of EBI experts in the later ELIfe-based FP7 lipidomics
application LipidomicNet.

Devising a strategy for lipidomics research (Objective 3)
Experts in three areas of development of lipidomics: i) technology, ii) cell biology, and iii)
health, were collected in three workshops to discuss progress in their subfield and to define
what would be the best way to stimulate lipidomics research in Europe. Experts in technology
were brought into contact with basic scientists and clinicians at an industry workshop, that
was positioned as a satellite meeting to the concluding ELIfe/FEBS special meeting in
Noordwijkerhout (NL). The outcome of this meeting, which attracted over 60 scientists, was a
round table discussion on technologies available, on instrument development, and on
application of these in society, where possible in high throughput mode. A second workshop
in Bilbao (E), cosponsored by EMBO, brought together basic scientists in physics, chemistry
and biology who focused on the theme of lipid rafts, membrane substructures of exciting
functions, and the possibilities to further characterize these structures using novel
technologies. In a final workshop on 'Lipidomics and Health' preceding the 2006 ICBL
meeting in Pécs (HU), life scientists and clinical scientists discussed the applications of novel
and high-throughput lipidomics technology in medical applications.

In line with the workshops, the following papers were published, each of which included a
conclusion/perspective section: 'Cellular Lipidomics', 'Lipidomic strategies to study structural
and functional defects of ABC-transporters in cellular lipid trafficking' and 'The European
Lipidomics Initiative: Enabling Technologies' [4-6]. In addition, a white paper under the title

                                                                                                6
'Enabling Technologies for Studying the Genome, Proteome and Cytome of the Lipidome'
was published as an interactive paper on the ELIfe website [7]. A series of editorials by ELIfe
members served to critically evaluate future developments in lipidomics [8-13]. In addition,
ELIfe members formed an interest group with other lipid scientists to prepare a policy
document for the European Science Foundation under the title: 'Structural Medicine II: the
Importance of Lipidomics for Health and Disease' [14].

To pursue possibilities to apply for lipidomics funding under FP7 two meetings were
organized in 2006 and 2007 for ELIfe members plus other interested scientists in Frankfurt
(D). This eventually resulted in the successful FP7 application LipidomicNet. A EuroCORE
theme proposal EuroMEMBRANE by a team led by the ELIfe chair has been accepted by
ESF, with an expected call for research grant applications in early 2008 [15].

Science-related and policy meetings (Objective 5)
A series of scientific and strategic meetings has been organised. Besides the technology
workshop in Dresden (2005), and the three workshops in Bilbao, Pécs an Noordwijkerhout,
ELIfe contributed sessions to four industrial and life science meetings: (1) the 2005
conference of the European Life Sciences Organization (ELSO 2005) in Dresden (D), was
attended by 1,200 scientists, amongst whom 40% PhD students. The program consisted of 6
plenary sessions, 21 minisymposia, 3 poster sessions and 7 sub-group meetings. ELIfe
contributed Minisymposium 1: Lipidomics. (2) the 26th World Congress and Exhibition of
the International Society for Fat Research (26th ISF World Congress) in Prague (CZ) 2005,
hosted by the Czech Chemical Society and Euro Fed Lipid: 'Modern aspects of fats and oils -
A fascinating source of knowledge'. The session on 'Lipid Bioscience and Genomics' to this
industrial meeting was sponsored by ELIfe. (3) the 47th International Conference on the
Bioscience of Lipids (ICBL - ELIfe – ILPS joint meeting), 2006, in Pécs (HU): two sessions
on 'Lipidomics' and 'membrane microdomains' to an audience of basic lipid scientists. (4) the
4th Euro Fed Lipid Congress, 'Fats, Oils and Lipids for a Healthier Future - The Need for
Interdisciplinary Approaches', Madrid (E), 2006. One session was contributed on 'Lipid
Mediators and Lipidomics'. This was a meeting with an industrial character.

The concluding general ELIfe meeting 'New concepts in lipidology: from lipidomics to
disease' in Noordwijkerhout, NL, 2006, was cosponsored as a FEBS special meeting and was
attended by over 250 participants The meeting hosted a short joint symposium between the
Nordrhein-Westfälische Akademie der Wissenschaften and the Dutch Royal Academy of Arts
and Sciences. A special ELIfe issue of FEBS Letters under the title 'Lipidome and Disease'
was distributed at the meeting [16].




                                                                                                7
2. Dissemination and use

Over 2005 and 2006, the specific support action 'the European Lipidomics Initiative (ELIfe)'
has resulted in four successful workshops where stakeholders from basic science, industry and
medicine met to discuss cell biological, technological, industrial and clinical aspects of
lipidomics. In addition, ELIfe contributed lipidomics sessions to four networking meetings,
one on life sciences, one on the bioscience of lipids and two industrial lipid meetings. These
workshops and meetings culminated in the concluding general meeting in October 2006,
attended by a broad audience of 250 scientists. At the occasion of this meeting a special
journal issue was published on 'Lipidome and Disease' [16]. The results of the the specific
support action were reported in a series of policy papers in the scientific literature [4-6, 8-13],
and a policy briefing on lipidomics and health of the European Science Foundation [14]. In
addition, ELIfe contributed to a number of technical papers on lipid classification and data
handling [1-3] and to a 500 page document entitled 'Enabling technologies for studying the
genome, proteome and cytome of the lipidome.' [7]. This document will be presented on the
Lipidomics Expertise Wiki Portal (LEP-Wiki): http://www-cgi.uni-
regensburg.de/Klinik/Klinische_Chemie/lipidWiki/, to allow a direct interaction with the
scientists in the field. Interviews with ELIfe members appeared in various journals [17,18].

A survey was prepared based on registrations in the Lipidomics Expertise Platform
www.lipidomics-expertise.de with the purpose of identifying stakeholders and providing
insight in the available expertise, which can be used for contacting specific centers for
collaboration. A 260 page document entitled 'Lipid droplets and lamellar bodies as dynamic
organelles connecting influx, efflux, and storage of lipids: Translational research towards
human disease' was prepared as the basis for a grant proposal under FP7 by a number of
ELIfe members plus other scientists. This proposal 'LipidomicNet' was recently selected for
funding. A EuroCore theme proposal under the name EuroMembrane [15] has been accepted
by the European Science Foundation and a call for applications is expected for early 2008.

In summary, we believe that the European Lipidomics Initiative has created many
opportunities for crossover between basic science and medical and commercial applications,
and that it has inspired stakeholders to seek contact and establish strategic alliances. Because
national funding agencies and policy makers value the European dimension, the project will
impact on both the European and the national level in shaping policies and research activities,
both in applied and fundamental research. One such field is that of nutrition and health.
Technology development will allow more detailed analyses of lipid patterns in diseased and
healthy persons, which will drive discussions with the food industry concerning the potential
positive and negative effects of different types of (lipid) nutrition on human health with new
health policies drawn up as a result.




                                                                                                 8
References

[1.a]   Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy,
        C.R. Raetz, D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer,
        M.S. Vannieuwenhze, S.H. White, J. Witztum, and E.A. Dennis (2005) A
        comprehensive classification system for lipids. J. Lipid Res. 46, 839-861.
[1.b]   idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
[2]     Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M.,
        Bogdanov, A., Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle,
        T., Jarv, J., Karyakin, A., Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I.,
        Spener, F., Voyer, N., and Wild, J. (2005) Postgenomic chemistry (IUPAC Technical
        Report). Pure Appl. Chem. 77, 1641–1654.
[3]     Spener F. (2005) Lipidomics and consequences: a new classification system for lipids.
        Eur. J. Lipid Sci. Technol. 107, 277-278.
[4]     van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.
[5]     Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study
        structural and functional defects of ABC-transporters in cellular lipid trafficking.
        FEBS Lett. 580, 5597-5610.
[6]     van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The
        European lipidomics initiative: enabling technologies. Methods Enzymol. 2007;432,
        213-232.
[7]     White paper on 'Enabling technologies for studying the genome, proteome and cytome
        of the lipidome.' to be published in Wikipedia format on the LEP website.
[8]     Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci.
        Technol. 107, 1-2.
[9]     Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–
        708.
[10]    Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci.
        Technol. 108, 895–897.
[11]    Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies
        – from innocent bystanders to prime targets of lipid research for combating human
        diseases. Eur. J. Lipid Sci. Technol. 108, 541-543.
[12]    Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J.
        Lipid Sci. Technol. 108, 625-627.
[13]    van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J.
        Lipid Sci. Technol. 108, 799–801.
[14]    van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U.,
        Bougnoux, P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., Julià-
        Sapé, M., Larijani, B., Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B.,
        Record, M., Schmitz, G., Simons, K., Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R.,
        Wakelam, M.J.O., and Wanders, R.J.A. (2008) Structural Medicine II: the Importance
        of Lipidomics for Health and Disease, European Science Foundation Policy Briefing,
        in press.
[15]    van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G.,
        Warren, G. (2008) Membrane Architecture and Dynamics (EuroMEMBRANE),
        EuroCORE theme proposal. Call to be launched in spring 2008.
[16]    Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special
        Issue. FEBS Lett. 580, 5429-5610.
[17]    Winckler, L. (2005) Lipidomics. Laborjournal 12, 20-23.
[18]    Hillyer, C.D. (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206-208.


                                                                                              9
10
                                  Qui ckT ime™ and a
                        T IFF (Uncompressed) decompressor
                            are needed to see thi s pi cture.




                                LSSG-CT-2004-013032
                                          ELIfe
                 European Lipidomics Initiative: Shaping the Life Sciences

Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6




       TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

Survey of expertise and infrastructure within the Lipidomics field



                                                      Final Report
                                                       2005-2007




Date of preparation: 1/1/08
Start date of project: 1/1/05                                                  Duration: 2.5 years
Project coordinator name:                                       Prof. Gerrit van Meer
Project coordinator organisation name:                          Utrecht University




                                                                                                     11
 Work Package 2 (coordinated by Partner 2: Prof. Gerd Schmitz, Regensburg)
                                      Deliverable 2.4:
        Survey of expertise and infrastructure within the Lipidomics field


The following project objectives were implemented into the Lipidomics
Expertise Platform (LEP, http://www.lipidomics-expertise.de) :
   -   Design an Expertise Platform on Lipidomics
   -   Hold a survey of expertise and infrastructure with the Lipidomics field


Description of the Lipidomics Expertise Platform (LEP)
The Lipidomics Expertise Platform (LEP, http://www.lipidomics-expertise.de) was set up in
2005 (Fig. 1).




Fig. 1: Starting page and menu structure of the LEP


In order to gain full access institutions and users have to register with the LEP. Currently,
128 different scientific and 6 industrial institutions are registered in the LEP. These
institutions include a total number of 188 registered users. Most users belong to 18 different
European countries, although there are non-European registrations from 8 countries (Table
1). The contact details of institutions and user registered in LEP are attached in appendices 1
and 3, respectively.


                                                                                            12
                                           Europe

                                           Users                      Institutions
           Country            scientific        industrial     scientific       industrial
            Austria              10                 1              8                1
           Belgium                4                                4
          Denmark                 1                                1
           Finland                6                                6
            France               21                   1           14                1
          Germany                33                               24
           Greece                 1                                1
           Hungary                7                                4
             Italy               14                                9
        Netherlands              21                   2            7                2
           Norway                 1                   1            1                1
            Poland                1                                1
          Romania                 1                                1
           Slovenia               1                                1
            Spain                10                                7
           Sweden                 5                                3
        Switzerland               4                                2
       United Kingdom            22                   1           16                1
           Total                 163                  6          110                6


                             Non European Countries

                                           User                       Institutions
          Country             scientific          industrial   scientific       industrial
         Argentina                2                                1
            Brazil                1                                1
          Canada                  2                                2
            China                 1                                1
            Israel                1                                1
            Japan                 3                                2
          Taiwan                  3                                2
        United States            12                                8
           Total                 25                   0           18                0
Table 1: Users and Institutions registered in the LEP (as at 01.01.2008)

One aim of the Lipidomics Expertise Platform was to collect and provide information on labs
involved in lipidomics. Therefore the LEP contains various electronic surveys that collect
contact information and give an overview of the expertise present in the various labs. The
institutions registered were asked to complete electronic surveys on (Fig. 2):
   -    Areas of lipidomics expertise
   -    Lipid class skills
   -    Technology skills
   -    Organisms and cellular systems skills

                                                                                             13
Fig. 2: Wizard for electronic surveys




Fig. 3: Search menu for lipidomics expertise.


                                                14
The results of these surveys are searchable in an advanced search menu (Fig. 3). A
statistical analysis of the survey results is shown in Table 2-5. Detailed survey results are
displayed in appendix 2 ordered by country.


                       Areas of Lipidomics Expertise
Lipid Metabolism                                       37
Analytical Tools in Lipidomics                         36
Lipid Signalling                                       23
Membrane Microdomains                                  22
Mapping of the Lipidome                                19
Genomics of the Lipidome                               19
Lipids in Inflammation                                 19
Metabolomics of the Lipidome                           18
Membrane Trafficking                                   18
Lipid Transport                                        17
Lipids and Nutrition                                   17
Signalomics of the Lipidome                            11
Bioinformatics related to the Lipidome                 10
Proteomics of the Lipidome                              9
Pharmacology and toxicology of the Lipidome             8
Glycomics of the Lipidome                               7
Lipids in Microbiology                                  7
Chemical Synthesis of Lipids (Probes/Tools/Agents)      7
Lipid Standard Bank                                     7
Nutrigenomics of the Lipidome                           6
Lipids in Skin Care                                     2
Table 2: Survey on the areas of Lipidomics expertise; Institutions with medium and high
expertise level

                               Lipid Class - Survey
Fatty Acids and Conjugates [FA01]                           24
Glycerophosphocholines [GP01]                               21
Glycerophosphoethanolamines [GP02]                          17
Eicosanoids [FA03]                                          13
Ceramides [SP02]                                            13
Diradylglycerols [GL02]                                     12
Glycerophosphoglycerols [GP04]                              11
Docosanoids [FA04]                                           8
Glycerophosphoglycerophosphates [GP05]                       7
Glycerophosphates [GP10]                                     7
Fatty aldehydes [FA06]                                       6
Fatty esters [FA07]                                          6
Triradylglycerols [GL03]                                     5
Phosphosphingolipids [SP03]                                  5
Neutral glycosphingolipids [SP05]                            5
Sterols [ST01]                                               5
Glycerophosphoserines [GP03]                                 4
Glycerophosphoinositols [GP06]                               4
Glycerophosphoinositol bisphosphates [GP08]                  4
Acidic glycosphingolipids [SP06]                             4
Bile acids and derivatives [ST04]                            4
Glycerophosphoinositol monophosphates [GP07]                 3
Steroids [ST02]                                              3
Table 3: Survey on lipid classes investigated; Institutions with medium and high expertise
level (only lipids classes with more than 2 hits)



                                                                                             15
                         Technology - Survey
ESI                                              32
Fluorescence Microscopy                          27
Enzymatic Assays                                 27
LC                                               24
GC                                               23
Gel Electrophoresis                              22
Light Microscopy                                 19
CI                                               18
Confocal Microscopy                              18
LC-MS coupling                                   17
NCI                                              15
Thin layer chromatography                        14
Normal Phase                                     14
GC-MS coupling                                   14
Real Time PCR                                    14
Reversed Phase                                   13
Ion Exchange                                     13
Liquid-Liquid Extraction                         13
Cell Sorting                                     13
Immuno Assays                                    13
DNA Sequencing                                   12
Data Processing                                  11
EI                                                9
Quadrupole                                        9
Ion Trap                                          9
Database Setup                                    9
Cluster Analysis                                  9
Biostatistics                                     9
Immune Affinity                                   8
MALDI                                             8
Solid Phase Extraction                            7
Microarrays                                       7
Chemical Synthesis                                7
Size Exclusion                                    6
nano ESI                                          6
APCI                                              6
Electron Microscopy                               6
Nuclear Magnetic Resonance                        6
Multicolor Analysis                               5
UV-Vis Spectroscopy                               5
TOF                                               4
Electron Spin Resonance                           4
Infrared Spectroscopy                             4
Fluorescence Phosphorescence Spectroscopy         4
Capillary Electrophoresis                         3
FTICR                                             3
QqTOF                                             3
Atomic Force                                      3
Capillary Electrophoresis                         3
dHPLC                                             3
Table 4: Survey on the technologies used; Institutions with medium and high expertise level
(only technologies with more than 2 hits)




                                                                                          16
             Organisms and cellular Systems - Survey
Human Cell Lines                                       27
Mice                                                   22
Blood                                                  22
Biopsy                                                 18
Human Primary                                          15
Mouse                                                  15
Rats                                                   14
Yeast                                                  14
Urine                                                  10
Eubacteria                                              5
Rabbits                                                 4
Arabidopsis                                             4
Agricultural Crop                                       3
Stool                                                   3
Table 5: Survey on the areas of Lipidomics expertise; Institutions with medium and high
expertise level (only organisms with more than 2 hits)



Beside this contact and expertise database the LEP contains databases for lipid standards
(Fig. 4) and for methods (Fig. 5), which should improve methodology and standard material
exchange.




Fig. 4: Methodology Database.




                                                                                          17
Fig. 5: Lipid Standard Database.


Currently, as one goal of the FP project LipidomicNet the Wikipedia software is being
integrated into the LEP (LEP-Wiki, Fig. 6), which will provide information on lipids from basic
information to advanced aspects like the biology and pathophysiology that lipids are involved
in.
A major source for LEP-Wiki is a close link to other databases including LIPID MAPS
(http://www.lipidmaps.org) for lipid nomenclature and lipid species data, Lipid Bank - Japan
(http://www.lipidbank.jp) for lipid species data and KEGG PATHWAY Database for metabolic
pathways    (http://www.genome.jp/kegg/pathway.html).      Moreover,   information   on   lipid
metabolic pathways will be linked to genomics information including databases like Ensembl
(http://www.ensembl.org).
LEP-Wiki lives from the contribution of people interested in Lipidomics. All Lipidomics
Expertise Platform (LEP) members can edit the content and add new data.




                                                                                            18
Fig. 6: LEP-Wiki




                   19
       Appendix 1: Contact details and description of institutions registered in LEP ordered by country (as per 01.01.2008)

Company            Organisation         Phone        ZIP      City        Street             Country     Keywords                   Description                                                                   Type
UNESCO Chair       Universidad          054          8000     Bahia       C.C. 857           Argentina   cell-surface receptors /   biochemistry and biophysics of lipids cell-surface membrane structure         scientific
Biophys. & Mol.    Nacional del Sur     2914861201            Blanca                                     lipid-protein              and dynamics lipid-protein interactions in nicotinic receptors lipids in
Neurobiol                                                                                                interactions /             embryonic development lipids in neuronal differentiation and survival
                                                                                                         membranes / structure      lipids in retina lipids in plants lipids in reproductive system
                                                                                                         / dynamics
Inst. Biophysics   Austrian Academy ++43-316-        A-8045   Graz        Schmiedlstrasse    Austria     lipid polymorphism,        Our research aims at the elucidation of the molecular mode of action of     scientific
& X-ray            of Sciences      4120-323                              6                              membrane biophysics,       host defence peptides that affect cells by interacting in a non-specific
Structure                                                                                                membrane mimetic,          manner with their membranes and not via specific receptors. An
Research / Fct.                                                                                          membranolytic              understanding of how these peptides distinguish between bacterial and
Lipidomics                                                                                               peptides                   mammalian cell membranes will allow the design of novel peptide
                                                                                                                                    antibiotics, which can selectively kill bacteria. Bacterial resistance to
                                                                                                                                    such antimicrobial peptides is less likely to occur owing to the nature of
                                                                                                                                    their target and fast killing kinetics. However, there has been evidence
                                                                                                                                    that resistance to antibiotics may also arise due to changes in lipid
                                                                                                                                    composition of their membranes. Therefore, the design of novel and
                                                                                                                                    effective antimicrobial peptides will only be possible, if the entire lipid
                                                                                                                                    spectrum of its membrane has been identified, which nowadays owing to
                                                                                                                                    recent advances in analytical instrumentation can be addressed both at
                                                                                                                                    the qualitative and quantitative level. Experimental approach -
                                                                                                                                    membrane biophysics (structural, thermodynamic and spectroscopic
                                                                                                                                    techniques)- lipid analysis of target membranes - peptide libraries
BIOCRATES          BIOCRATES Life       ++43-512-    6020     Innsbruck   Innrain 66         Austria     Metabolomics, Lipid        Targeted metabolomics Mass spectrometry Quantitation of phospho-            industrial
Life Sciences      Sciences             579823-                                                          quantitation,              and glycolipids, eicosanoids etc. Bioinformatics and biostatistics
                                        4216                                                             Bioinformatics             Biomarker discovery and validation
Institute for      Graz University of   ++43-316-    8010     Graz        Petersgasse 14     Austria     transcriptional            Transcriptional regulation of lipotoxic pathways. Gene expression           scientific
Genomics and       Technology           873-5345                                                         regulation,                analysis of mouse models of lipid-associated disorders.
Bioinformatics                                                                                           microarrays,
                                                                                                         adipogenesis,
                                                                                                         lipotoxicity
Institute for      Graz University of   ++43-316-    A-8010   Graz        Petersgasse 14/I   Austria     yeast lipids, sterols      Lipid transport in yeast The roles of sterols in membrane transport in        scientific
Molecular          Technology           873-4089                                                                                    yeast Sterol homeostasis in yeast
Biotechnology
Institute of       Graz University of   +43-316-     A-8010   Graz        Petersgasse 12/2   Austria     yeast, mitochondria,       The main subjects studied in our group are synthesis of lipids and their      scientific
Biochemistry,      Technology           873-6462                                                         lipid particles,           assembly into organelle membranes of the yeast Saccharomyces
Cell Biology                                                                                             phospholipids, neutral     cerevisiae. The majority of yeast lipids are synthesized in the
Group                                                                                                    lipids                     endoplasmic reticulum with some significant contributions of
                                                                                                                                    mitochondria, the Golgi and the so-called lipid particles. Other
                                                                                                                                    subcellular fractions, e.g. the plasma membrane, are devoid of lipid-
                                                                                                                                    synthesizing activities. Spatial separation of lipid biosynthetic steps and
                                                                                                                                    lack of lipid synthesis in several cellular membranes necessitate an
                                                                                                                                    efficient transfer of lipids from their site of synthesis to their proper
                                                                                                                                    destination(s). The maintenance of organelle lipid profiles requires strict
                                                                                                                                    coordination and regulation of biosynthetic and translocation processes.



                                                                                                                                                                                                          20
                                                                                                                                     Specific aspects currently studied are the assembly of lipids into
                                                                                                                                     mitochondrial membranes and lipid homeostasis in this compartment,
                                                                                                                                     and dynamics of neutral lipid storage in lipid particles. Recently we
                                                                                                                                     started to extend our studies to organelles of Pichia pastoris as a basis
                                                                                                                                     for future research of protein expression in this biotechnologically
                                                                                                                                     important yeast. Enzymes and other proteins involved in the above
                                                                                                                                     mentioned processes are investigated using biochemical, cell biological
                                                                                                                                     and molecular biological methods.
Structural          Inst. of Chemistry   +43-316-     8010     Graz     Heinrichstr. 28      Austria   membrane protein,             We are working on the characterization of a typ IV-like secretion system scientific
biology                                  3805423                                                       conjugation, T4SS             (T4SS) from Gram positive bacteria. The system is encoded on the
                                                                                                                                     resistance plasmid pIP501. The tra region shows a modular organization
                                                                                                                                     and contains 15 ORFs, of which several have been predicted to encode
                                                                                                                                     for transmembrane or membrane asociated proteins. Our aim is the
                                                                                                                                     structure elucidation of the components essential for the conjugative
                                                                                                                                     DNA transfer.
Institute of        Joanneum             ++43316876   8036     Graz     Auenbruggerplatz     Austria   acyl-coA, mass                analysis methods based on GC-MS, HPLC-MS^2 and nano-HPLC-MS^2 scientific
Medical             Research             2103                           20/3                           spectrometry,                 analysis of interstitial fluid of humans and animals method validation
Technologies                                                                                           interstitial fluid analysis   according to GLP guidelines
and Health
Mangement
Molecular           Medical University +433163804     A-8010   Graz     Harrachgasse 21      Austria   cholesterol            Novel and already identified genes involved in lipid homeaostasis of                scientific
Biology and         Graz               200                                                             metabolism, nuclear    different organs including liver, intestine, endothelial cells,
Biochemistry,                                                                                          receptors,             macrophages-foam cells. Cholesterol efflux and cholesteryl ester
                                                                                                       atherosclerosis        hydrolases. Pathophysiology of athersoclerosis. Lipid metabolism in
                                                                                                                              gneral. Lipidome of human plasma in health and diseases. Lipidome of
                                                                                                                              enterocytes and cardiomycytes .
IMB                 University of Graz   ++43 316     A8010    Graz     Schubertstr. 1       Austria   yeast, fatty acid,     Lipid metabolism and membrane assembly in yeast, yeast as a model of                scientific
Biochemistry -                           380 5487                                                      membrane and lipid     lipid-associated disorders, regulation of fatty acid, triglyceride and
Yeast Genetics                                                                                         imaging                phospholipid metabolism, high-resolution microscopy, implementation of
Group                                                                                                                         novel imaging methods to investigate membrane and organelle stucture
                                                                                                                              and dynamics in yeast, yeast lipidomics
Institute of        Vienna University    ++43 1      A-1060    Vienna   Getreidemarkt        Austria   MALDi, ESI, multistage Characterisation of lipid pattern in human blood during dietary treatment           scientific
Chemical            of Technology        58801 15160                    9/164                          MS, plant lipids,      Characterisation of lipid pattern of industrial (as a renewable source of
Technologies                                                                                           human blood            chemicals), pharamceutical and medical relevant plants Development of
and Analytics,                                                                                                                ultrafast and structurespecific MALDI and ESI mass spectrometric
Bio and Polymer                                                                                                               techniques for all classes of lipids
Analysis
Core Facility for   ZMF/Medical          ++43 (316)   8010     Graz     Stiftingtalstrasse   Austria   Mass Spectrometry,            Development of mass spectrometric tools for the analysis of lipids           scientific
Mass                University Graz      385-73005                      24                             FT-MS, Glycerolipids,         (neutral and polar glycerolipids, sphingolipids, cholesterols) and lipid
Spectrometry                                                                                           Sphingolipids                 derived second messengers (e.g. eicosanoids) is a key research interest
                                                                                                                                     of our facility. This is reflected by participation in center grants such as
                                                                                                                                     SFB Lipotox and the technology based initiative Lipidomic Research
                                                                                                                                     Center (LRC) Graz. Currently we develop an analytical platform based
                                                                                                                                     on UPLC-FT-MS/MS for differential quantitation of the lipidome at the
                                                                                                                                     level of molecular species. Finally these data should enable researchers
                                                                                                                                     to establish up- and down regulated metabolic pathways for different
                                                                                                                                     sets of samples. If it is needed to provide absolute quantitative data for
                                                                                                                                     single species or a limited set of them, we have the possibility to quantify




                                                                                                                                                                                                           21
                                                                                                                                  them in a targeted approach with LC-MS/MS.
Div.               K.U.Leuven          ++32-16-     B-3000    Leuven         Campus              Belgium   phytanic, pristanic,   Breakdown of fatty acids/derivatives via alpha-oxidation and beta-              scientific
Pharmacology -                         345801                                Gasthuisberg,                 ceramide, sphingosine- oxidation, peroxisomal lipid metabolism, bioactive sphingolipid
Dep. Mol. Cell                                                               Herestraat                    phosphate              metabolism, mouse models related to peroxisomal disorders,
Biology
CMPG-PFI           Katholieke          ++32 16 32   3001      Heverlee       Kasteelpark         Belgium   yeast, sphingolipid,      We are interested in sphingolipidomics in yeast. Using ESI-MS, we            scientific
                   Universiteit        96 88                                 Arenberg 20                   ESI-MS                    have optimized a method to determine and relatively quantify the three
                   Leuven                                                                                                            different classes of complex inositolphosphoryl-containing sphingolipids
                                                                                                                                     [i.e. IPC (inositolphosphoryl ceramide), MIPC (mannosyl
                                                                                                                                     inositolphosphoryl ceramide) and M(IP)2C (mannosyl
                                                                                                                                     diinositolphosphoryl ceramide] in S. cerevisiae [Aerts et al., 2006, FEBS
                                                                                                                                     Letters, 580(7):1903-7]. We are interested in technologies enabling
                                                                                                                                     quantification of sphingoid bases and ceramides in yeast membranes.
Laboratory for     Katholieke          ++32-16-     3000      Leuven         Herestraat 49 bus   Belgium   cancer, lipid rafts,      Our team is interested in the metabolic changes in cancer cells versus       scientific
Experimental       Universiteit        330533                                902                           protein acylation         normal cells, and particularly in the marked increase in lipogenesis that
Medicine and       Leuven                                                                                                            is observed in nearly all cancer types. We 1. study the mechanisms that
Endocrinology                                                                                                                        underly this increase (imvolvement of oncogenes, steroid hormones,...),
(LEGENDO)                                                                                                                            2. We examine the impact of these chnages on lipid profiles, lipid rafts
                                                                                                                                     and lipid-modified proteins, 3. We investigate the consequences of these
                                                                                                                                     changes for cancer cell biology (growth, metastasis,...), and 4. We
                                                                                                                                     explore the potential clinical applications (diagnosis, therapy).
Neuronal           VIB and Catholic    +32-16-      3000      Leuven         Herestraat 49       Belgium   cholesterol lipid rafts   We are interested in analysing the role of cholesterol in the cell           scientific
Differentiation    University of       330526                                                              senescence                senescence/survival process. We postulate that cholesterol reduction in
Unit               Leuven                                                                                                            membrane lipid rafts it might be a central effector in survival of
                                                                                                                                     senescent neurons, implying that brain cholesterol regulation could be
                                                                                                                                     crucial for death/survival equilibrium durin brain aging.
Laboratório de     Departamento de     55-51-33-16- 90.035-   Porto Alegre   Rua Ramiro          Brazil    shingolipids,             - Sphingolipid metabolism and their cellular functions in central nervous    scientific
Bioquímica e       Bioquímica -        55-50        003                      Barcelos 2600-                gangliosides,             system and hematopoietic differentiation. - Adipogenesis and
Biologia Celular   ICBS-                                                     anexo                         adipogenesis,             lipogenesis.
de Lipídios        Universidade                                                                            lipogenesis
                   Federal do rio
                   Grande do Sul
Research           Hospital for Sick   1-416-813-   M5G1X     Toronto        555 University      Canada    sulfatide,                Structural organization of glycosphingolipids and effect of length of fatty scientific
Institute, Boggs   Children            5919         8                        Ave.                          galactosylceramide,       acid chain and hydroxylation of fatty acid, Lipid rafts and membrane
Laboratory                                                                                                 glycosynapse              domains in myelin and oligodendrocytes and role in signalling, Trans
                                                                                                                                     interactions between glycosphingolipid head groups and formation of a
                                                                                                                                     glycosynapse between apposed cell membranes, Role of
                                                                                                                                     phosphatidylinositides.
CIHR Group in      University of       1-780-492-   T6G       Edmonton       328 Heritage     Canada       atherosclerosis,          Goals of our CIHR Group in Molecular and Cell Biology of Lipids (MCBL) scientific
Molecular and      Alberta             2963         2S2                      Medical Research              obesity,                  * To enhance the knowledge and understanding of the metabolism,
Cell Biology of                                                              Centre                        neurodegeneration,        function and transport of mammalian lipids, lipid biosynthetic enzymes
Lipids                                                                                                     lipids                    and transport proteins, and the regulation of the genes that encode
                                                                                                                                     these proteins * To facilitate the translation of discoveries into potential
                                                                                                                                     diagnostics and treatments of human diseases * To provide
                                                                                                                                     fundamental knowledge that will improve the health of Canadians and
                                                                                                                                     peoples throughout the world * To provide an environment that will
                                                                                                                                     enhance scholarly and scientific endeavors. The principal investigators
                                                                                                                                     * Luis B. Agellon, PhD, Associate Professor of Biochemistry * Gordon




                                                                                                                                                                                                          22
                                                                                                                         A. Francis, MD, Associate Professor of Medicine * Richard Lehner,
                                                                                                                         PhD, Associate Professor of Pediatrics and Cell Biology * Dennis E.
                                                                                                                         Vance, PhD, Professor of Biochemistry * Jean E. Vance, PhD,
                                                                                                                         Professor of Medicine Our research program The MCBL Group research
                                                                                                                         program is organized into four major themes: * molecular regulation of
                                                                                                                         genes involved in lipid homeostasis * biochemistry of lipid-protein
                                                                                                                         interactions * lipid compartmentalization and intracellular trafficking *
                                                                                                                         lipid homeostasis in murine models The current major research projects
                                                                                                                         are: * regulation of phosphatidylcholine metabolism * molecular and
                                                                                                                         cell biology of phosphatidylserine metabolism * triacylglycerol
                                                                                                                         synthesis * role of triacylglycerol hydrolase in triacylglycerol
                                                                                                                         metabolism * function and metabolism of sterols in the liver *
                                                                                                                         metabolism of lipids in the enterohepatic circulation * cellular lipid
                                                                                                                         efflux and HDL formation * lipid homeostasis in neurons Our Core
                                                                                                                         Resources Members of the MCBL Group contribute specific technical
                                                                                                                         expertise and newly developed technologies which are shared with other
                                                                                                                         projects and laboratories through our Core resources. * The Core Cell
                                                                                                                         Culture Laboratory provides access to a variety of cell lines (including
                                                                                                                         various derivatives of McArdle RH7777 cells expressing recombinant
                                                                                                                         forms of enzymes important in the metabolism of lipids), and technical
                                                                                                                         support for immunofluorescence and confocal microscopy studies. *
                                                                                                                         The Core Metabolism and Physiology Laboratory provides access to
                                                                                                                         genetically-modified mouse strains used by investigators in the MCBL
                                                                                                                         Group. This laboratory also provides access to primary cell cultures, in
                                                                                                                         conjunction with the Core Cell Culture Laboratory, and technical support
                                                                                                                         for surgical procedures. * The Core Lipid Analysis Laboratory provides
                                                                                                                         access to current, standardized and validated analytical procedures, and
                                                                                                                         technical support for the detection and analysis of lipids and their
                                                                                                                         metabolites. * The Core Training Program provides opportunities for
                                                                                                                         qualified postdoctoral fellows and graduate students to participate in our
                                                                                                                         integrated research program.
Lab 305          Institute of       86-10-     100101   Beijing   Datun            China      lipid, gene cloning,       I am interested in improvement of oilcrops, lipid synthesis related gene   scientific
                 Genetics and       64889783                      Road,Chaoyang               gene function              cloning and gene function characterization.
                 Developmental                                    District                    characterization
                 Biology, Chinese
                 Academy of
                 Sciences
Biochemistry     The Technical      +45        2800     Lyngby    Building 224, DTU Denmark   LC/MS, animal              Research areas/competencies related with lipidomics: Identification and scientific
and Nutrition    University of      45252744                                                  experimental facilities,   quantification of triacylglycerols and phospholipids by LC/MS, separation
Group,           Denmark                                                                      GC, TLC                    and quantification of different phospholipids and lipid classes by NP-
Biocentrum-DTU                                                                                                           HPLC, separation and quantification of triacylglycerol molecular species
                                                                                                                         by RP-HPLC, separation and purification of lipids by TLC and SPE,
                                                                                                                         analysis of fatty acid profiles by GC, analysis of lipid regiostructures by
                                                                                                                         partial degradation (Grignard degradation for triacylglycerols &
                                                                                                                         phospholipase degradation for phospholipids) followed with GC, GC/MS,
                                                                                                                         LC/MS, analysis of plasma lipids by Cobas Mira, quantification of
                                                                                                                         cholesterol in different lipoproteins using HPLC. Other research
                                                                                                                         activities: Lipid absorption and metabolism, application of stable isotope
                                                                                                                         techniques and GC/C/IRMS in metabolic studies, effect of dietary fats on



                                                                                                                                                                                              23
                                                                                                                                  learning ability and visual function, bioavailability of n-3 fatty acids,
                                                                                                                                  applying enzymatic technology in studies on lipid modification including
                                                                                                                                  structured triacylglycerols and structured phospholipids, studies on new
                                                                                                                                  dietary lipids such as diacylglycerols and conjugated linoleic acids,
                                                                                                                                  interactions of lipids with other food components, lipoprotein oxidation,
                                                                                                                                  development of analytical methods, especially emphasized on
                                                                                                                                  separation techniques and mass spectrometry for analysis of lipids and
                                                                                                                                  lipid soluble vitamins, qualitative and quantitative analysis of
                                                                                                                                  carbohydrates. We possess extensive expertise in lipid biochemistry and
                                                                                                                                  lipid analysis, and we have the following facilities: Analytical instruments
                                                                                                                                  for lipid analysis of food and biological systems (GC, HPLC, TLC,
                                                                                                                                  GC/MS, LC/MS, GC/C/IRMS, Cobas Mira, Spectrophotometer) Animal
                                                                                                                                  experimental facilities
University of    Department of        +358 50 563   00020    Helsinki    PO Box 700         Finland   liver insulin marker        Profiling of human serum in individuals with a fatty vs no-fatty liver in      scientific
Helsinki         Medicine             6899                                                                                        collaboration woth Matej Oresic.
Helsinki         Institute of         +358-9-       FIN-     Helsinki    POB 63             Finland   lipids, drugs, proteins,    lipid phase behavior, bioactive lipids, lipid-protein interactions, drug-lipid scientific
Biophysics &     Biomedicine          19125400      00014                                             amyloids                    interactions, cationic lipids in gene delivery, lateral organization of
Biomembrane                                         Universi                                                                      biomembranes
Group                                               ty of
                                                    Helsinki
Institute of    University of         ++358-9-      P.O.     Helsinki    Haartmaninkatu     Finland   Lipid-protein               -Interactions of lipid membrane and membrane proteins/ion channels -           scientific
Biomedicine/Käk Helsinki              19125409      Box 63               8/P.O. Box 63                interactions, Mass          Cellular metabolism of lipids and fatty acids -Mass spectrometry of lipids
elä Group                                                                                             spectrometry, Ion
                                                                                                      channels, Fatty acids
Institute of     University of        358-9-        00014    Helsinki    Haartmaninkatu     Finland   mass-spectrometry,          1)Lipid trafficking 2)Regulation of lipid composition of cells (lipid          scientific
Biomedicine/So   Helsinki             19125410                           8/PL 63                      trafficking,                homeostasis) 3)Mode of action of phospholipases 4)Membrane
merharju group                                                                                        membrane,phospholip         structure, expecially lateral distribution of lipids 5)Atherosklerosis and
                                                                                                      ase                         other lipid related diseases 6)Development of MS tools for lipid analysis
Department of    University of Oulu   +358-8-553    FIN-     Oulu        POB 3000           Finland   mitochondria,               Lipids and metabolic disorders, yeast lipids, fatty acid synthesis,            scientific
Biochemisty                           1150          90014                                             peroxisomes, structural     membrane lipidomics, lipid metabolism, lipid metabolizing enzymes,
                                                                                                      enzymology
Quantitative     VTT Technical        ++358-20-     FIN-     Espoo       Tietotie 2, P.O.   Finland   systems biology, early      Animal model phenotype characterization using lipid (and general            scientific
Biology and      Research Centre      720-4491      02044                Box 1500                     markers,                    metabolite) profiling approaches - Bioinformatics method for integration
Bioinformatics   of Finland                         VTT                                               metabolomics,               of lipid profile information from mass spectrometry based approches with
                                                                                                      phenotype                   other levels of data such as gene expression and protein profiles -
                                                                                                      characterization            Clinical applications of lipidomics for early disease detection and
                                                                                                                                  disease progression studies, focus on diabetes
Biological       Biological           ++33-1-       78850    Thiverval   BP 1               France    oil, oil bodies, A          We are interested in understanding the biology of oil bodies, with special scientific
Chemistry        Chemistry            30815474               Grignon                                  thaliana, Y lipolytica, B   emphasis on assembly and degradation. We are currently studying two
Laboratory       LaboratoryNation                                                                     napus                       organisms: - A. thaliana, a model for for oil crop. -- Y. lipolytica, yeast
                 al Institute for                                                                                                 capable to use and transform lipids. We combine a multiscale approach
                 Agronomic                                                                                                        going from: -the identification of oil bodies proteins (proteomics,
                 Research (INRA)                                                                                                  enzymatic studies, immunochemistry) --the study of isolated proteins
                                                                                                                                  (purified of recombined) in solution (enzymology, interfacial properties
                                                                                                                                  using hanging drop methods), or within model interfaces (Langmuir
                                                                                                                                  balance experiment) --- the study of proteins within complete organelle
                                                                                                                                  (from wt or mutants organisms) ----the study of wt and mutant model
                                                                                                                                  organism. ----- transcriptomic analysis of yeasts genes expressed or




                                                                                                                                                                                                          24
                                                                                                                           repressed upon shift from glucose to oleic acid medium. -Our goals are
                                                                                                                           - to identify all protein component involved in oil bodies biogenesis -- to
                                                                                                                           understand their role and identify their activity ---to study the influence
                                                                                                                           of oil body protein composition on lipid content (nature, amount)
                                                                                                                           Principal collaborations: -Within France Dr. M Miquel, Dr. L Lepiniec,
                                                                                                                           Seed Biology Laboratory, INRA, Versailles: Molecular biology of A.
                                                                                                                           thaliana seeds, Dr JM Nicaud Molecular Genetic Laboratory, INRA
                                                                                                                           CNRS, Grignon: Molecular biology of Y. lipolytica. Dr. M Axelos: BIA
                                                                                                                           Laboratory, INRA, Nantes. Interfacial methods -Within European Union
                                                                                                                           Prof. G Daum, Dr K Athensdaedt, technical University, Graz: Lipid
                                                                                                                           particles from yeast Dr. S Papanikolaou, Agronomic University of
                                                                                                                           Athens, Biotechnology of Oleaginous yeast
INSERM-538       CHU Saint-      33-01-        75571   PARIS 12°   27 rue Chaligny       France   membrane,                Membrane heterogeneity and polarized cellular traffic. Cholesterol and        scientific
(biomembranes)   Antoine         40011340                                                         microdomains, traffic,   sphingolipids enriched membrane microdomains Rotavirus traffic in
                                                                                                  phospholipids            Caco-2 cells. Synthesis of bile phospholipids
Laboratory of    CNRS            33 4 91 16    13402   Marseille   31 chemin Joseph France        lipases, TLC-FID,        Lipolytic enzymes and their use for the characterization lipids Production scientific
Enzymology at                    41 34                 cedex 20    Aiguier                        monolayers               facilities, purification and kinetic characterization of several recombinan t
Interfaces and                                                                                                             lipases, including gastric and pancreatic lipases, pancreatic lipase-
Physiology of                                                                                                              related proteins, hormone-sensitive lipase, microbial lipases, Plant
Lipolysis                                                                                                                  phospholipase D, phospholipase A2, phospholipase A1, galactolipase
                                                                                                                           Use of thin-layer chromatography coupled to flame ionization detection
                                                                                                                           (TLC-FID, Iatroscan) for quantitative measurement of neutral and polar
                                                                                                                           lipid species and their lipolysis products. Use of the monomolecular film
                                                                                                                           technique for studying lipid-protein intercations and lipolytic enzymes
                                                                                                                           kinetics. This technique allows measurement of lipolytic activity with very
                                                                                                                           low amounts of substrate (g)and is therefore suitable for rare lipids.
CIML             CNRS INSERM     33            13288   marseille   parc sxcientific de   France   abc transporters,        lipid transport /flip by abac transporters of the A class lipidomic analysis scientific
                                 491269404                         luminy                         abca1, malaria,          of microparticles during malaria infection
                                                                                                  flippase
Laboratoire de   CNRS UMR 5200   +33 (0)5 57   33076   Bordeaux    146 Rue Léo           France   Plant lipids, waxes,     The Membrane Biogenesis Laboratory (MBL) has a long history of                  scientific
Biogenèse        Université V.   57 10 45              cedex       Saignat                        fatty acids, GC, Lipid   research in membrane biogenesis and lipid biosynthesis. This lab is
Membranaire      Segalen                                                                          analysis                 internationally known and has been recently chosen to organise the
                 Bordeaux 2                                                                                                International Plant Lipid Symposium in 2008. The aims of the research
                                                                                                                           concern the identification and the characterisation of the genes involved
                                                                                                                           in the regulation of lipid metabolism. Using Arabidopsis thaliana as the
                                                                                                                           main plant model, the topics currently developed in the unit are: i/
                                                                                                                           research project I: Genomics of epicuticular wax metabolism. The
                                                                                                                           objective is to characterise the genes involved in the biosynthesis of
                                                                                                                           epicuticular waxes in order to understand the regulatory mechanisms of
                                                                                                                           the expression of genes, which control the composition of the wax layer
                                                                                                                           in response to various stress conditions. ii/ research project II:
                                                                                                                           Membrane biogenesis and homeostasis. This project aims to
                                                                                                                           characterise the mechanisms controlling the membrane lipid
                                                                                                                           composition, the lipid transfer between organelles and membranes, and
                                                                                                                           the lipid composition of lipid rafts in relation to hormonal signalling. iii/
                                                                                                                           research project III: SNAREs, lipids and endomembrane dynamics. The
                                                                                                                           objective is to study the role of SNAREs in membrane dynamics
                                                                                                                           between the ER and the Golgi apparatus in the secretory pathway




                                                                                                                                                                                                   25
                                                                                                                                    leading to the biogenesis of plasmalemma and the formation of lipid rafts
                                                                                                                                    through the secretory pathway. The MBL is one unit of the IFR 103
                                                                                                                                    Integrative Biology which involves 6 labs covering the different areas of
                                                                                                                                    plant research (pathogenesis, biotechnology, agronomy), and
                                                                                                                                    technological platforms (imaging, lipidomic, metabolism, transcriptome).
                                                                                                                                    The MBL has in charge the lipidomic platform which consist of 3 GLC,1
                                                                                                                                    GC/-MS 1 densitometer, 1 phosphor imager and 2 TLC spotters. This
                                                                                                                                    platform allows lipid and fatty acid analysis and lipid metabolism studies.
Institute of        CNRS UMR6097        ++33-(0)4-    06560   Valbonne       Sophia Antipolis-   France   phospholipase A2, lipid Eleven genes coding for secreted phospholipases A2 (sPLA2s, 14-18             scientific
molecular and                           93957733                             660 route des                mediator, cancer,         kDa) and 2 types of receptors (M and N) have now been identified in
cellular                                                                     lucioles                     inflammation              mammalian tissues. sPLA2s and their receptors are found in several
pharmacology                                                                                                                        tissues, and their expression levels are increased in inflammation,
                                                                                                                                    associated diseases, different types of cancer, and neurodegenerative
                                                                                                                                    diseases. Although the biological functions of sPLA2s and their
                                                                                                                                    receptors are still ill-defined, some sPLA2s are mitogenic and/or
                                                                                                                                    apoptotic, pro-inflammatory and/or pro-tumoral, but also anti-tumoral,
                                                                                                                                    anti-bacterial, anti-viral, and anti-parasitic. At the molecular level,
                                                                                                                                    sPLA2s are most likely bifunctional proteins, acting as both enzymes
                                                                                                                                    and ligands for a variety of soluble and membrane proteins. sPLA2
                                                                                                                                    enzymatic activity participates in the control of the rate-limiting step in
                                                                                                                                    the production of lipid mediators such as prostaglandins and
                                                                                                                                    leukotrienes which are involved in a myriad of biological effects. We
                                                                                                                                    have previously identified for the first time the M and N type receptors
                                                                                                                                    for sPLA2s using snake venom sPLA2s as ligands. More recently, we
                                                                                                                                    have cloned 7 of the 11 known mammalian sPLA2s, and we have shown
                                                                                                                                    that several of these enzymes are the natural ligands of the M-type
                                                                                                                                    receptor, suggesting that mammalian sPLA2s are true ligands for
                                                                                                                                    mammalian proteins. The main objective of our research program is to
                                                                                                                                    determine the function of sPLA2s, while continuing their molecular
                                                                                                                                    characterization. Our specific aims are : 1) To analyze the molecular
                                                                                                                                    properties of sPLA2s and to develop key tools to determine sPLA2
                                                                                                                                    function (recombinant production, native structure, crystallization,
                                                                                                                                    enzymatic and binding properties, search of specific inhibitors,
                                                                                                                                    characterization of receptors, transgenic mice), 2) To analyze the tissue
                                                                                                                                    distribution of sPLA2s and their receptors in normal and pathological
                                                                                                                                    states in relation with the following item, 3) To study the role of sPLA2s
                                                                                                                                    in three major research areas : colorectal cancer, inflammation, and host
                                                                                                                                    defense against viruses, bacteria, and parasites.
INSERM              Faculté de          +33 1 40 61   75015   Paris          156 rue de          France   lipid rafts, eicosanoids, Protein-lipid interactions, proteomics and lipidomics of lipid              scientific
U467/IFR94          Médecine René       56 21                                Vaugirard                    epithelium                microdomains, and their relevance in physiopathology of epithelial cells.
Proteomics          Descartes Paris 5                                                                                               Regulation of inflammation in cystic fibrosis.
Platform
French Institute    Industrial          +33 5 56 36   33600   PESSAC         rue Monge           France   nutrition bioavailability   Nutritional impact of lipids (in relation to diseases : CHD, cancer,        industrial
for Fats and Oils   Technical Center    00 44                                                             cancer CHD                  obesity). Biomarkers studies (adipose tissue, plasma, erythrocytes).
(ITERG)
IMBL-Lipid          INSA-Lyon and   33-4-72 43        69621   Villeurbanne   20 Ave A.           France   Signaling , Fatty acids , INSERM UMR 585 / INSA-Lyon is part of IMBL (Institute for                     scientific
Signaling (UMR      CNRS / INSERM / 82 40                                    Einstein                     Lipid mediators ,         Multidisciplinary Biochemistry of Lipids). This UMR works on lipid
585 INSERM /        Univ Lyon1                                                                            Analysis                  signaling, in blood, vascular cells and adipocytes, in the context of




                                                                                                                                                                                                             26
INSA-Lyon)                                                                                                                 aging, atherosclerosis, diabetes and obesity. Lipids of interest are
                                                                                                                           membrane phospholipids as reservoirs of polyunsaturated fatty acids
                                                                                                                           which serve as precursors of eicosanoids and docosanoids. The
                                                                                                                           production of those oxygenated metabolites through cyclooxygenase
                                                                                                                           and lipoxygenase pathways is the main focus. The role of oxidative
                                                                                                                           stress/lipid peroxidation in those pathways as well as their control by
                                                                                                                           polyunsaturated fatty acids of nutritional value are of special interest.
                                                                                                                           The sphingomyelin / ceramide / sphingosine / sphingosine-1-phosphate
                                                                                                                           is also taken into consideration.
INSERM U671      INSERM           ++33142346    75006   Paris      15 rue de l ecole   France   adipocyte obesity lipid    Synopsis of current research interest in the field of lipid droplet biology:   scientific
                                  923                              de medecine                  droplet                    We are working in the field of metabolic diseases associated with
                                                                                                                           obesity in an INSERM unit in Paris (UMR 671). In these diseases,
                                                                                                                           ectopic lipid storage is a key deleterious event at the whole body level,
                                                                                                                           associated with grave metabolic complications. In this regard, we are
                                                                                                                           interested in lipid trafficking in adipose cells specialized in the storage of
                                                                                                                           fat. Caveolins have been found at the surface of lipid droplets, but their
                                                                                                                           functional role in the dynamics of fat storage remains unknown. Since
                                                                                                                           caveolins are highly expressed in adipocytes, and caveolin-KO mice
                                                                                                                           have revealed a striking inability to store fatty acids from extracellular
                                                                                                                           sources into their adipose tissue, we focused our research on the role of
                                                                                                                           caveolins in lipid homeostasis. In a recently published paper (Le Lay et
                                                                                                                           al., Traffic 2006), we have shown that adipocyte lipid droplet
                                                                                                                           composition was dependent on caveolin expression and that exogenous
                                                                                                                           cholesterol was able to induce caveolin targetting to these lipid droplets
                                                                                                                           through a process sharing many features in common with caveolar
                                                                                                                           endocytosis. We are now interesting in elucidating the dynamic
                                                                                                                           relationship between cell surface and lipid droplets through caveolin
                                                                                                                           trafficking, and identifying the adipocyte lipid species that can be stored
                                                                                                                           through a caveolin dependent pathway. In this line, our future projects
                                                                                                                           deal with the functional role of caveolins in lipid targeting to the fat
                                                                                                                           storage compartment, the lipid droplet.
INSERM U586      INSERM,          33-5-         31432   Toulouse   CHU Rangueil        France   lysophosphatidic acid,     My group is interested in the regulations and the biological                   scientific
(Obesity                          62172956                         BP84225                      autotaxin,                 consequences of the release of lysophosphatidic acid (LPA) by
Research Unit)                                                                                  lysophospholipase D,       adipocytes. We are particularly interrested in a lysophospholipase D
                                                                                                adipocyte,                 (autotaxin) which appears to be the main source of LPA release by
                                                                                                                           adipocytes but which could also be involved in synthesis of several other
                                                                                                                           bioactive phospholipids. ATX expressio is adipose tissue is up-
                                                                                                                           regulated in association with insulin-resistance and type 2 diabetes. In
                                                                                                                           paralelle, we are also interrested in understanding the transduction
                                                                                                                           pathways (particularly the receptors) involved in LPA actions on the
                                                                                                                           different cell types surrounding adipocytes in the adipose tissue
                                                                                                                           including preadipocytes, macrophages, endothelial cells.
IFR30 Lipid      INSERM/Toulous   33            31024   Toulouse   CPTP Bat C,         France   neutral lipid, ceramide,   Our Lipid Analysis Platform belongs to the French Nationale Institute for scientific
Analysis         e Genopole       (0)56177941                      Hopital Purpan               bile acid, phytosterol     Health and Medical Research (INSERM). It is located in the Federative
Platform                          4                                BP3028                                                  Research Institute (IFR30) in Toulouse, France and is associated to the
                                                                                                                           Toulouse Genopole. The platform created in 2003 proposes a number of
                                                                                                                           lipid analysis using chromatographic methods, either adapted from the
                                                                                                                           litterature or original. We are equiped with 3 gas chromatographs (GC)
                                                                                                                           with FID detection, one GC coupled to a mass spectrometer and one



                                                                                                                                                                                                   27
                                                                                                                              liquid chromatograph (HPLC) coupled to a light scattering detector
                                                                                                                              (LSD). We propose qualitative and quantitative analysis of lipid
                                                                                                                              molecules following extraction from micro-samples (biological fluids,
                                                                                                                              tissues, cells). We thus have access to the molecular species of the
                                                                                                                              following lipids : - Neutral lipids including cholesterol and related sterols,
                                                                                                                              diacylglycerol, cholesterol ester and triacylglycerol (on the basis of
                                                                                                                              carbon number) - Free or total fatty acids - Sphingomyelines and usual
                                                                                                                              ceramides - Phytosterols - Bilary acids (conjugated or free) - We are in
                                                                                                                              progress to propose in the near future the analysis of major
                                                                                                                              phospholipids classes, galactolipids and cerebrosides by HPLC-LSD.
                                                                                                                              Analysis are proposed on the basis of a price per sample. Collaboration
                                                                                                                              on specific projects is opened. Present ongoing projects include the
                                                                                                                              fields of intestinal lipid absorption, cancer and apoptosis, lipoprotein
                                                                                                                              metabolism, nervous system functionnning, lipid transduction
Microbiology    National Institute ++33         F78850   Thiverval-    CBAI B.P. 01       France   yarrowia lipolytica, lipid We are interested in understanding the degradation of hydrophobic                scientific
and Molecular   for Agronomic      130815450             Grignon                                   accumulation, genetic      substrates (alkanes, fatty acids, oils) by the oleaginous yeast Yarrowia
Genetic         Research (INRA)                                                                                               lipolytica. We combine a multistage approach going from: -
Laboratory,     and National                                                                                                  transcriptional analysis of gene expression during growth on
UMR2585         Centre for                                                                                                    hydrophobic substrates using Y lipolytica micro array, - the identification
                Scientific                                                                                                    of peroxisomal and oil bodies proteins (proteomics), - the study of wild-
                Research (CNRS)                                                                                               type and mutants affected in HS utilisation, in beta oxidation, in lipid
                                                                                                                              accumulation, ... Our goals are to identified genes involved in lipid
                                                                                                                              accumulation (functional analysis, nature and amount of fatty acid
                                                                                                                              accumulated), lipid and HS transport into different cell organelles.
                                                                                                                              Principal collaboration: - within France Dr T Chardot, Biological
                                                                                                                              Chemistry, INRA, Grignon (biochemistry of lipid bodies), Prof N Latruffe,
                                                                                                                              Burgundy University, Dijon (peroxisome), MC C Molina-Jouve, INSA,
                                                                                                                              Toulouse (Biotechnology of oleaginous yeast), - within European Union:
                                                                                                                              Prof G Daum, Dr Athensdaedt, Technical University, Graz (lipid particles
                                                                                                                              from yeast), Dr S Papanicolaou, Agronomic University of Athens
                                                                                                                              (Biotechnology of oleaginous yeast).
Cyberlipid      Private           3304674143    34090    Montpellier   2 rue du colonel   France   database internet lipids Construction and administration of an internet site devoted to all lipid           scientific
Center                            65                                   Marchand                                               aspects www.cyberlipid.org which is yet cited in your links page
Nutrition,      Université        +33 (0)247    37044    TOURS         CHU Bretonneau     France   diet, cancer, lipidome,    In the field of cancer prevention through dietary lipids, we use the white       scientific
Croissance et   François Rabelais 366179                                                           PUFA, CLA                  adipose tissue lipid composition as a qualitative indicator of past dietary
Cancer,                                                                                                                       intake of lipids. Through a reappraisal of white adipose tissue data
INSERM E211                                                                                                                   obtained recently in human and animal models, we individualized a
                                                                                                                              composite lipid profile associated with a low risk of breast cancer. This
                                                                                                                              profile has led us to elaborate a composite index indicative of the risk of
                                                                                                                              breast cancer. This biomarker offers the opportunity to quantify the part
                                                                                                                              due to modifiable dietary factors in the risk of breast cancer and
                                                                                                                              complements the currently known risk factors for breast cancer.
                                                                                                                              Bougnoux P, Giraudeau B, Couet C. Diet, cancer and the lipidome.
                                                                                                                              Cancer Epidemiology, Biomarkers & Prevention 2006, 15(3):416-21.
University of   University        +33 3 80 39   21000    DIJON         6 Bd Gabriel       France   Diacylglycerols, MAP       Dietary polyunsaturated fatty acids (PUFA) have been classified into two         scientific
Bourgogne                         63 12                                                            kinases, T cells,          categories, belonging to n-6 and n-3 families. These fatty acids are
                                                                                                   Macrosomia                 indispensable for animal cell to maintain its structure, fluidity and
                                                                                                                              function. The polyunsaturated fatty acids (PUPA) of n-3 family have




                                                                                                                                                                                                      28
                                                                                                                                  been considered as authentic immunosuppressors, however, their
                                                                                                                                  mechanisms of action in T-cell activation have not been well elucidated.
                                                                                                                                  T-cell activation involves a series of complex mechanisms from
                                                                                                                                  membrane receptor to gene transcription via the second messenger
                                                                                                                                  cascades. The main theme of our research is to elucidate the role of
                                                                                                                                  dietary PUPA of n-3 family in human T-cells activation which might be
                                                                                                                                  involved in the pathology of several diseases like diabetes and obesity.
                                                                                                                                  We are studying the interaction of these fatty acids with the second
                                                                                                                                  messenger cascade / cell signalling (MAP kinases, calcium signalling,
                                                                                                                                  protein kinases C & diacylglycerol).
Applied           Applera            +496151967   64293   Darmstadt    Frankfurter         Germany   SOP, MS, LCMS,               Co-Developement of Gene Expression Assays Standard Operating                     industrial
Biosystems        Deutschland        05231                             Strasse 129B                  funding, TaqMan              Procedures for MS Lipid Analysis Business developement / Product &
                  GmbH                                                                               Assays                       Service developement in Lipidomics
AkdÄ              Arzneimittelkomm   ++49304004   10623   Berlin       Herbert-Lewin-      Germany   Clinical effects of lipid    Clinical effects of lipid lowering, outcome trials, statins, safety of statins   scientific
                  ission der         56500                             Platz 1                       lowering, outcome
                  deutschen                                                                          trials, statins, safety of
                  Ärzteschaft                                                                        statins
Julius-v-Sachs-   Biocenter,         ++49-931-    D-      Wuerzburg    Julius-von-Sachs-   Germany   plant oxylipins,             We are interested in the functional analysis of plant lipids. There is a   scientific
Institute for     University of      888-6160     97082                Platz 2                       isoprostanes                 long track record on plant oxylipins of the jasmonate type. However
Biosciences,      Wuerzburg                                                                                                       more recently we came interested in oxidized lipids derived from non-
Pharmaceutical                                                                                                                    enzymatic oxidation of plant membrane lipids. The current focus is on
Biology                                                                                                                           hydroxy fatty acids and prostaglandin-like plant isoprostanes termed
                                                                                                                                  phytoprostanes. Formation of these compounds in response to
                                                                                                                                  environmental stress conditions is intensively been investigated in
                                                                                                                                  cyanobacteria and plants. It has been shown by our group that oxidized
                                                                                                                                  lipids (including phytoprostanes)represent archetype signals that induce
                                                                                                                                  detoxification and defence responses in plants and cyanobacteria.
                                                                                                                                  Moreover oxidized plant lipids also have an pronounced effect on
                                                                                                                                  mammalian immune cells. In the future, a comprehensive analysis of
                                                                                                                                  plant oxidized lipids and their biological effects in vivo (cyanobacteria,
                                                                                                                                  plants) will be performed. The lab is equipped with three GC-MS (sector
                                                                                                                                  field MS and quadrupol MS instruments). More recently, an Nano-ESI-
                                                                                                                                  HPLC-Ion-Trap MS instrument for plant proteome analysis and a HPLC-
                                                                                                                                  Triple Quadrupol MS for plant lipidomics have been purchased.
Eicosanoids and   Deutsches        49 6221        D       Heidelberg   Im Neuenheimer      Germany   Lipoxygenase,                The long-term objective of the research group is to substantially          scientific
Tumor             Krebsforschungsz 424506         69120                Feld 280                      ichthyosis, epidermal        contribute to the understanding of the role of eicosanoids, which perform
Development       entrum                                                                             barrier                      important tasks as local signals coordinating the concerted activities of
                                                                                                                                  different cell types in tissues under normal and disease conditions with a
                                                                                                                                  special focus on cancer. As autocoids the biological activities of
                                                                                                                                  eicosanoids are primarily determined by their biosynthesis via the
                                                                                                                                  cyclooxygenase (COX) and the lipoxygenase (LOX) pathways.
                                                                                                                                  Accordingly, the expression and activity of COX and LOX are strictly
                                                                                                                                  regulated under normal conditions, transiently up- or down-regulated
                                                                                                                                  during irritation and tissue regeneration, and permanently deregulated
                                                                                                                                  during carcinogenesis in both humans and mice. The group’s
                                                                                                                                  experimental strategy is to establish expression and activity profiles of
                                                                                                                                  individual LOX in mouse and human biopsies in order to bridge the
                                                                                                                                  animal data with clinical investigations. The experimental models used




                                                                                                                                                                                                           29
                                                                                                                             for functional studies include phorbol ester-irritated or wounded mouse
                                                                                                                             skin, the two-stage skin carcinogenesis approach, and transgenic
                                                                                                                             mouse lines with targeted over-expression or deletion LOX genes in skin
                                                                                                                             epidermis or other epithelia in vivo and epithelial cells in vitro. Our
                                                                                                                             current work is focussed on analyses of molecular mechanisms involved
                                                                                                                             in the functions of epidermis-type LOX in tissue homeostasis and
                                                                                                                             disease-related deregulation of eicosanoid metabolism.
Deutsche           DGPF              089-1897    82152    Martinsried   Am Klopferspitz   Germany   Proteomics, proteome     Proteomics                                                              scientific
Gesellschaft für                     9007                               18                          research
Proteomforschu
ng
department of      DKFZ(German       49-6221-    D-       Heidelberg    Im Neuenheimer    Germany   glucosylceramide         Glycosphingolipid and immune function Glycosphingolipid and              scientific
cellular and       Cancer Research   424350      69120                  Feld 280                    synthase,cerebrosides    transgene mice
molecular          Center)                                                                          ulfotransferase,cell-
pathology                                                                                           specific
                                                                                                    deficiency,fertilify
Dept. of         Eberhard Karls      #49-(0)7071- 72076   Tübingen      Calwerstr. 7      Germany   Phospholipids, in vivo   The Lipid Metabolism and Developmental Physiology Group focuses on scientific
Neonatology,     Universität         29-86377                                                       metabolism, D9-          clinical as well as on developmental and comparative aspects of the
Lipid Metabolism Tübingen                                                                           choline, human           metabolism of individual (phospho)lipid molecular species. Main topics
and                                                                                                                          are: (1) the metabolism of pulmonary phospho- and other lipids, together
Developmental                                                                                                                with that of their hydrophobic and hydrophilic proteins, in relation to
Physiology                                                                                                                   pulmonary maturation, ante- and perinatal diseases and the
Group                                                                                                                        development of bronchopulmonary dysplasia. (2) the function of
                                                                                                                             individual and characteristic phospholipid molecular species with respect
                                                                                                                             to air-liquid interface function under dynamic conditions and to
                                                                                                                             immunological differentiation of immune-competent cells in terminal lung
                                                                                                                             tissues. (3) the metabolism and secretion of hepatic phospholipids and
                                                                                                                             their role in the supply of peripheral organs with lipids and lipid
                                                                                                                             precursors during postnatal development and at different clinical
                                                                                                                             settings, where liver function is compromised. To investigate these
                                                                                                                             issues we have performed a lipidomic approach to individual
                                                                                                                             phospholipid molecular species composition and metabolism of
                                                                                                                             pulmonary surfactant systems. This includes investigation across a wide
                                                                                                                             spectrum of vertebrate species in relation to development and their
                                                                                                                             differing parameters of pulmonary physiology, air-liquid interface
                                                                                                                             dynamics and parenchymal structures (avian air capillaries, mammalian
                                                                                                                             sacculi, alveoli and airways). Furthermore, it includes investigation of
                                                                                                                             functional characteristics of individual phospholipid components for
                                                                                                                             phagocyte differentiation and function as well as for surface tension
                                                                                                                             function. We have furthermore studied the relation of pulmonary
                                                                                                                             surfactant molecular composition to extrapulmonary interfaces, like that
                                                                                                                             of the hydrophobic gastric barrier. To investigate phospholipid
                                                                                                                             metabolism under different clinical conditions and during postnatal
                                                                                                                             development we have developed and established strategies using stable
                                                                                                                             isotope labelled precursors in vivo in human subjects as well as in
                                                                                                                             animal models, combined with electrospray-ionization tandem mass
                                                                                                                             spectrometric (ESI-MS/MS) analysis of hydrophilic phospholipid
                                                                                                                             precursors and their synthesis products. We have furthermore
                                                                                                                             established animal models suitable for the investigation of inflammatory



                                                                                                                                                                                               30
                                                                                                                                   and hereditary diseases and during development, where changes of
                                                                                                                                   pulmonary and/or hepatic phospholipid metabolism as well as that of
                                                                                                                                   lipid-derived mediators is prominent (bronchopulmonary dysplasia,
                                                                                                                                   hyperoxic lung injury, cystic fibrosis).
Albrecht-von-      Georg-August-       ++49-551-    37077   Goettingen   Justus-von-Liebig   Germany   Plant lipid metabolism,     A central aspect in lipid metabolism is lipid peroxidation. It is common to   scientific
Haller-Institute   University          39-5743                           Weg 11                        lipid peroxidation          all biological systems, both appearing in developmentally and
for Plant                                                                                              processes, oxylipins,       environmentally regulated processes. Products are hydroperoxy
Sciences/Plant                                                                                         &#946,-oxidation            polyunsaturated fatty acids and metabolites derived there from
Biochemistry                                                                                                                       collectively named oxylipins or eicosanoids in mammals. They may
                                                                                                                                   either originate from chemical oxidation or are synthesized mainly by the
                                                                                                                                   action of various highly specialized forms of lipoxygenases. We are
                                                                                                                                   analyzing the physiological function of oxylipins during the degradation
                                                                                                                                   of storage lipids in oilseed seedlings as well as the involvement of
                                                                                                                                   lipoxygenases in the interaction of plants with pathogenic fungi and
                                                                                                                                   bacteria. Another research focus is the ana- and catabolism of lipid
                                                                                                                                   peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty
                                                                                                                                   acids, aldehydes, ketols and divinyl ethers by combined biochemical and
                                                                                                                                   analytical methods as well as by using a metabolomic approach (oxylipin
                                                                                                                                   profiling). The use of plants as bioreactors for biotechnological purposes
                                                                                                                                   is addressed by isolating new fatty acid desaturases, acyl transferases
                                                                                                                                   and double bond isomerases in order to introduce new functional groups
                                                                                                                                   in seed oils for nutritional and industrial purposes. In order to identfy
                                                                                                                                   bottle necks within these projects we use lipid profiling methods.
Albrecht-von-      Georg-August-       ++49-551-    37077   Goettingen   Justus-von-Liebig   Germany   Plant lipid metabolism,     A central aspect in lipid metabolism is lipid peroxidation. It is common to   scientific
Haller-Institute   University          39-5743                           Weg 11                        lipid peroxidation          all biological systems, both appearing in developmentally and
for Plant                                                                                              processes, oxylipins,       environmentally regulated processes. Products are hydroperoxy
Sciences/Plant                                                                                         &#946,-oxidation            polyunsaturated fatty acids and metabolites derived there from
Biochemistry                                                                                                                       collectively named oxylipins or eicosanoids in mammals. They may
                                                                                                                                   either originate from chemical oxidation or are synthesized mainly by the
                                                                                                                                   action of various highly specialized forms of lipoxygenases. We are
                                                                                                                                   analyzing the physiological function of oxylipins during the degradation
                                                                                                                                   of storage lipids in oilseed seedlings as well as the involvement of
                                                                                                                                   lipoxygenases in the interaction of plants with pathogenic fungi and
                                                                                                                                   bacteria. Another research focus is the ana- and catabolism of lipid
                                                                                                                                   peroxides by analyzing the biosynthesis of hydro(pero)xy polyenoic fatty
                                                                                                                                   acids, aldehydes, ketols and divinyl ethers by combined biochemical and
                                                                                                                                   analytical methods as well as by using a metabolomic approach (oxylipin
                                                                                                                                   profiling). The use of plants as bioreactors for biotechnological purposes
                                                                                                                                   is addressed by isolating new fatty acid desaturases, acyl transferases
                                                                                                                                   and double bond isomerases in order to introduce new functional groups
                                                                                                                                   in seed oils for nutritional and industrial purposes. In order to identfy
                                                                                                                                   bottle necks within these projects we use lipid profiling methods.
Department of      GKSS Research       ++49-4152-   21502   Geesthacht   Max-Planck-Str. 1   Germany   peptide antibiotics,        interaction in or at membrane interfaces sensing of biophysical               scientific
Macromolecular     Center              87-1291                                                         membrane biophysics,        membrane properties by proteins modification of biophysical membrane
Structure                                                                                              scattering                  properties by proteins, peptides or membrane active molecules peptide
Research                                                                                                                           antibiotics, viral infectivitity
Institute of       Humboldt-           49 (0) 30    D-      Berlin       Invalidenstr. 42    Germany   lipid, transport, fusion,   The research is interdisciplinary ranging from structural biology,            scientific
Biology/Molecul    University Berlin   2093 8860    10115                                              virus                       molecular and cell biology, virology, spectroscopy to the design and




                                                                                                                                                                                                         31
ar Biophysics                                                                                                       synthesis of biomolecule analogues. The topics of basic and applied
                                                                                                                    research are protein-mediated fusion of membranes (viral fusion
                                                                                                                    proteins), virus budding, lipid-trafficking in eukaryotic cells, protein-lipid
                                                                                                                    interaction in membranes, and application of membranes in
                                                                                                                    nanobiotechnology. Cell Entry and Budding of Enveloped Viruses: A
                                                                                                                    focus of our research is an early step of cell entry of enveloped viruses.
                                                                                                                    Enveloped viruses as influenza virus or HIV fuse with respective
                                                                                                                    membranes to deliver their genome into the host cell. In our lab
                                                                                                                    enveloped viruses are employed to study the molecular mechanism of
                                                                                                                    protein-mediated virus-membrane fusion. We use various biophysical
                                                                                                                    and cell biology methods to follow directly the fusion process and to
                                                                                                                    identify structural intermediates mainly by fluorescence microscopy and
                                                                                                                    spectroscopy. To understand the functioning of viral fusion proteins,
                                                                                                                    specific mutants and chimeras of those proteins are created and probed
                                                                                                                    for their fusion activity. By strong collaboration with groups specialised in
                                                                                                                    electron microscopy and in image reconstruction, we identify the three
                                                                                                                    dimensional structure of complete viral fusion protein at conditions
                                                                                                                    typical for triggering membrane fusion. Another research focus is the
                                                                                                                    assembly and budding of enveloped viruses. We are interested in
                                                                                                                    mechanisms underlying local enrichment of viral components at the
                                                                                                                    budding site of the host membranes. Lipid-trafficking in eukaryotic cells:
                                                                                                                    Lipids play an essential role in various cellular processes including
                                                                                                                    signal transduction. Our specific interest is the protein-mediated
                                                                                                                    translocation of lipids across cellular membranes and their intracellular
                                                                                                                    transport to distinct organelles. Although indirect evidence already exists
                                                                                                                    the molecular identification of translocases pumping on the expense of
                                                                                                                    energy (specific) lipids across membranes and of flippases facilitating a
                                                                                                                    rapid, energy independent and unspecific movement of lipids in
                                                                                                                    membranes is still awaiting. We are very much interested in the
                                                                                                                    physiological relevance of those protein-mediated lipid transports and
                                                                                                                    their function(s), for example in cell genesis, apoptosis, fertilisation, cell
                                                                                                                    shape, exo- and endocytosis, in the enrichment of specific lipids in the
                                                                                                                    bile fluid as well as in the mechanism and consequences of the lipid
                                                                                                                    transport mediated by multi drug resistance proteins in tumor cells.
Microbial         Institute for    +492461612   52426   Juelich   Stetternicher    Germany   lipolytic enzymes,     - Biochemistry and biotechnology with lipases, esterases,                      scientific
Expression        Molecular        947                            Forst                      biotechnology,         phospholipases, thioesterases - Biodetergents, e. g. rhamnolipids from
Technology        Enzyme                                                                     biodetergents          Pseudomonas aeruginosa - Membrane processes in pathogenic
                  Technology,                                                                                       bacteria
                  Heinrich-Heine
                  University
                  Duesseldorf
LIPIDOMIX         LIPIDOMIX        ++49-30-     D-      Berlin    Berliner Allee   Germany   eicosanoids,           Analytic of eicosanoid related substances like: Prostaglandins                 industrial
GmbH              GmbH             76778940     13088             261-269                    phospholipids, HPLC,   Leukotrienes Hydroxy- and Epoxy fatty acids High throughput analytics
                                                                                             mass spectrometry      Replacement of immuno assays and: Fatty acids Phospholipids,
                                                                                                                    Sphingolipids Lipid profiling using: HPLC - Triple quad mass
                                                                                                                    spectrometry
Walther-Straub-   Ludwig-          +49-89-      80336   Munich    Goethestr. 33    Germany   inflammation,          Arachidonic acid metabolism, pharmacology of inflammation,                     scientific
Institute         Maximilians-     21807564                                                  eicosanoids, obesity   neurotransmitter, obesity
                  University



                                                                                                                                                                                            32
Max Planck           Max Planck         ++49-331-     14476   Golm         Am Mühlenberg 1     Germany   galactolipid,             Biochemistry and Molecular Biology of Plant Lipids: Galactolipids,               scientific
Institute of         Society            567-8259                                                         tocopherol,               Tocopherol, Phylloquinone, Chloroplast Lipids, Phospholipids, Fatty
Molecular Plant                                                                                          phylloquinone             Acids
Physiology/Plant
Lipid Group
Institute of         Medical Faculty,   ++49-341-     04107   Leipzig      Härtelstrasse 16-   Germany   MALDI, 31P NMR,           We are using using MALDI-TOF mass spectrometry and 31P NMR                       scientific
Medical Physics      University of      9715733                            18                            Phospholipids,            spectroscopy for the evaluation of physiologicallly relevant
and Biophysics       Leipzig                                                                             Lysophospholipids         phospholipids. Besides methodological work, we are particularly
                                                                                                                                   interested in - the activity determination of phospholipases and
                                                                                                                                   phosphoinositde kinase - the lipid / phospholipid composition of tissues
                                                                                                                                   and body fluids - the changes of the lipid patterns under inflammatory
                                                                                                                                   conditions (e.g. in rheumatoid arthritis and atherosclerosis) - the
                                                                                                                                   changes of the lipid pattern of cells during differentaition
Institute for     RWTH Aachen           +49-(0)241-   52074   Aachen       Worringerweg 1      Germany   plant glycerolipid        our research focuses on plant lipid metabolism with special interest in          scientific
Biology I, Botany University            8026641                                                          isoprenoid                formation/function of glycerolipids (glycerophospholipids) and
                                                                                                                                   isoprenoids.
Institute for        RWTH-Aachen        ++49241808    52074   Aachen       Pauwelstr. 30       Germany   acyl-CoA, ceramide,       Functional characterisation of acyl-CoA synthetase and its products,             scientific
Pathology,                              9729                                                             liver                     esp. in gastrointestinal tract and liver. Cellular localisation, concentration
University                                                                                                                         and function of acyl-CoAs and lipids.
Hospital Áachen
Kekulé Institut f.   Universität Bonn   ++49 228      53121   Bonn         Gerhard-Domagk- Germany       Sphingolipids,            The research of Konrad Sandhoffs group focuses on sphingolipids and        scientific
Organische                              735346                             Straße 1                      Glykosphingolipids,       sphingolipidoses. Metabolism of sphingolipids and glycosphingolipids, its
Chemie und                                                                                               Sphingolipidoses, Lipid   function, regulation and topology are studied in vitro at the cellular and
Biochemie                                                                                                transfer proteins         organismic level. Sphingolipid disorders are analyzed to clarify human
                                                                                                                                   diseases and their molecular and cellular pathogenesis. At the
                                                                                                                                   biochemical level, we synthesize sphingolipid probes and analogues to
                                                                                                                                   analyze their molecular environment in the cells, and to characterize
                                                                                                                                   enzymes and sphingolipid activator proteins involved in sphingolipid
                                                                                                                                   metabolism and membrane digestion at lipid-water interphases. We
                                                                                                                                   develop lipid transfer assays for the analysis of lipid transfer between
                                                                                                                                   membranes by transfer proteins.
Kekulé-Institute     University Bonn    ++49-228-     53121   Bonn         Gerhard-Domagk- Germany       sphingosine-1-            A)Sphingosine-1-phosphate signaling in terminally differentiated post-     scientific
for Organic                             732703                             Str. 1                        phosphate, ceramide-      mitotic neurons and its implications for the pathology of
Chemistry and                                                                                            1-phosphate,              neurodegenerative disorders. B)The pro-inflammatory functions of
Biochemistry                                                                                             neurodegeneration,        sphingosine-1-phosphate and ceramide-1-phosphate and its
                                                                                                         postoperative ileus.      implications in postoperative ileus.
Department of        University         (+49) 6221    69120   Heidelberg   INF 410             Germany   Acyl-CoA synthetases,     Acyl-CoA synthetases in fatty acid uptake Lipid droplet formation Lipid    scientific
Gastroenterolog      Heidelberg         5638960                                                          Lipid droplets, Rafts,    rafts in fatty acid uptake Lipid rafts in intestinal inflammation Lipid MS
y                                                                                                        inflammatory bowel        analysis of intestinal mucus
                                                                                                         disease
Insitute of          University         ++49-341-     04103   Leipzig      Liebigstraße 27     Germany   phytosterols,           Sterol lipids: sterol metabolismn and atherosclerosis. Application of LC-          scientific
Laboratory           Hospital Leipzig   9722407                                                          oxysterols, eicosanoids MS/MS for the measuerment of free and esterified phtyosterols,
Medicine,                                                                                                                        lanosterol and cholesterol in serum and tissue. Eicosanoids: Evaluation
Clinical                                                                                                                         of a LC-MS/MS method for the measurements of eicosanoids as marker
Chemistry and                                                                                                                    of oxidative stress and inflammation.
Molecular
Diagnostics
Institute of         University of      ++49-221-     50931   Cologne      Joseph-             Germany   lipid droplets,           Our interest covers the analysis of lipid droplet associated proteins and        scientific




                                                                                                                                                                                                            33
Biochemistry I      Cologne              4783656                            Stelzmann-Str. 52             biosensor technology,      their function in lipid turnover. Coming from the field of membrane
                                                                                                          membrane protein           protein trafficking we apply cell based assay based on life cell imaging
                                                                                                          trafficking, endocytosis   and conventional confocal immunofluorescence techniques to localize
                                                                                                                                     proteins. We further have a long-standing expertise in the analysis of
                                                                                                                                     biomolecular interaction analysis using surface-plasmon-based
                                                                                                                                     biosensors (BIAcore 3000, T-100). We analyse protein-protein
                                                                                                                                     interactions as well as membrane protein interactions by this very
                                                                                                                                     sensitive method.
Biocenter Klein     University of        +49-(0)40-    D-      Hamburg      Ohnhorststr. 18     Germany   sphingolipid,              Molecularbiology and biochemistry of plant and fungal lipids: Functional     scientific
Flottbekand         Hamburg              42816-343     22609                                              desaturase,                characterizations of sterol-, sphingolipid and glycerol lipid-modifying
Botanical                                                                                                 glycosyltransferase        enzymes such as glycosyltransferases and desaturases/hydroxylases.
Garden/Plant
Physiology
Institut of         University of jena   ++49-03641-   D-      Jena         Dornburger Str.     Germany   metabolism, CLA,           fatty acid metabolism, CLA, omega-3 LC-PUFA, sterol metabolism               scientific
Nutrition                                949610        07743                24                            omega-3, sterol
Institut for Food   University of        +49 251 83    48149   Muenster     Corrensstr. 49      Germany   HPLC-MS/MS                 HPLC-MS/MS analysis of sphingolipids Influence of fumonisins on the          scientific
Chemistry           Muenster             33867                                                            fumonisin sphingolipids    sphingolipid metabolism
                                                                                                          food ceramide
                                                                                                          synthase
Leibniz-Institute   University of        +49 251       48149   Münster      Domagkstr. 3        Germany   coronary heart             Research on biomarkers related to chd, stroke, Alzheimer disease risk.       scientific
of                  Münster              8356181                                                          disease, biomarkers,       Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.
Arteriosclerosis                                                                                          NMR, mass
Research                                                                                                  spectrometry
Leibniz-Institute   University of        +49 251       48149   Münster      Domagkstr. 3        Germany   coronary heart             Research on biomarkers related to chd, stroke, Alzheimer disease risk.       scientific
of                  Münster              8356181                                                          disease, biomarkers,       Specific interest in technologies: H1-NMR, MS-MS, LC-MS, GC-MS.
Arteriosclerosis                                                                                          NMR, mass
Research                                                                                                  spectrometry
Department of       University of        +49 941       93051   Regensburg   Universitätsstr. 84 Germany   alpha-synuclein,           membrane microdomains and alpha-synuclein interaction in synaptic            scientific
Neurology           Regensburg           9410                                                             Parkinson,                 vesicle dynamics and plasticity
                                                                                                          Neurodegeneration,
                                                                                                          synaptic plasticity
Institute for       University of        ++49-941-     93053   Regensburg   Franz-Josef-        Germany   ABC Transporter, Lipid     The research of the institute is directed towards the molecular analysis     scientific
Clinical            Regensburg           944-6201                           Strauss-Allee 11              Biomarker, Biobanking      of metabolic and degenerative diseases. The central role of lipids in the
Chemistry                                                                                                                            pathogenesis of these disorders is a the main focus of the Institute of
                                                                                                                                     Clinical Chemistry. Our strategy combines basic research with clinical
                                                                                                                                     association studies enabling a rapid transfer of newly identified
                                                                                                                                     candidate markers from basic science to clinical diagnostics in order to
                                                                                                                                     support prediction, prevention, and therapy of diseases. The clinical part
                                                                                                                                     involves the Regensburg Diabetes Endpoint Prediction and Prevention
                                                                                                                                     Study (REDEPPS), an interdisciplinary platform that enables the
                                                                                                                                     establishment of large sample and data banks from patients with
                                                                                                                                     different endpoints or co-morbidities of diabetes, such as coronary heart
                                                                                                                                     disease, stroke, hypertension, obesity, nephropathy, retinopathy and
                                                                                                                                     neuropathy. Candidate markers which are verified for their diagnostic
                                                                                                                                     applicability in these cohorts are derived from basic research project
                                                                                                                                     focusing on the regulation of lipid homeostasis and vesicular traffic as
                                                                                                                                     related to disease pathogenesis. In this context the group has recently
                                                                                                                                     identified the ATP binding cassette transporter ABCA1 as a major




                                                                                                                                                                                                          34
                                                                                                                            regulator of HDL metabolism. The function of ABCA1 in the formation of
                                                                                                                            lipid microdomains and vesicular transport is subject of a current project
                                                                                                                            within the Transregional Collaborative Research Centre 6031 entitled
                                                                                                                            Membrane Microdomains and Their Role in Human Disease funded by
                                                                                                                            the DFG. The available technologies include high throughput genotyping
                                                                                                                            platforms and DNA-arrays for genetic analysis, protein analysis by
                                                                                                                            MALDI-TOF and the 2D-Gel Typhoon system, multicolour flow cytometry
                                                                                                                            and fluorescence imaging as well as tandem mass spectrometry and
                                                                                                                            NMR spectroscopy for lipids analysis. The methodological expertise of
                                                                                                                            the institute is complemented by the competence centre for fluorescent
                                                                                                                            bioanalysis (KFB) a public private partnership involving various institutes
                                                                                                                            of the University of Regensburg.
Department of     University of     ++49-511-     D-       Hannover   Bünteweg 17         Germany   Lipid microdomains,     Membrane transport, polarized protein sorting: role of lipid                scientific
Physiological     Veterinary        953 8780      30559                                             early detergent-        microdomains/detergent-resistant membranes, Intestinal membrane
Chemistry         Medicine                                                                          resistant membranes,    topology in IBD
                  Hannover                                                                          polarized protein
                                                                                                    sorting, colitis
Laboratory of     University of     +3026510-     451 10   Ioannina   Panepistemioupol    Greece    Lung surfactant,       Analysis of lung surfactant lipids and their interaction with surfactant        scientific
Lipid Analysis    Ioannina          98367                             is                            apoptosis,             proteins Lipids as substrates for phospholipases: metabolism, structural
                                                                                                    inflammation           studies, modeling Lipid modifications Lipids and signal transduction
                                                                                                                           Techniques in Lipid Analysis: TLC, gas chromatography, HPLC (normal
                                                                                                                           and reversed phase), Mass spectrometry (FAB, EI), Light scattering
Laboratory of    Department of      +36 30 320    4012     Debrecen   Nagyerdei Krt. 98   Hungary   HPLC MS-MS, retinoic HPLC MS-MS of retinoids, carotenoids and PUFA metabolites (HETEs,                 scientific
Nutritional      Biochemistry and   97 69                                                           acid, carotenoid, PUFA HODEs, eicosanoids).
Bioactivation    Molecular                                                                          metabolite
and Bioanalysis  Biology,
                 University of
                 Debrecen
Laboratory of    Institute of       +36-62-432-   H-6726   Szeged     Temesvari krt. 62. Hungary    stress sensing, lipid   Regulation of heat shock gene expression by the physical state, lipid          scientific
Molecular Stress Biochemistry,      048                                                             molecular species,      composition, mass spectrometry of lipids, microdomain organization of
Biology          Biological                                                                         mass spectrometry,      membranes in different systems, the mechanism of thermoadaptation,
                 Research Centre,                                                                   rafts                   stress sensing and signaling, cellular thermosensors, etc.
                 HUng. Acad. Sci.
Dept of Human    Semmelweis         36 1 215      1094     Budapest   Tuzolto u. 58       Hungary   caveoae, lipid rafts    In my lab we are very much interested in caveolae, mainly the function         scientific
Morphology and University           6924/3610                                                                               of these special lipid raft
Developmental
Biology
Dept. Biophysics University of      ++36-52-      4010     Debrecen   Egyetem ter 1.      Hungary   P-glycoprotein, lipid   We wish to understand the relationships between catalytic activity,            scientific
and Cell Biology Debrecen           455866                                                          rafts, cytoskeletal     conformational changes and topological constraints of the ABC
                                                                                                    anchorage               transporter P-glycoprotein (Pgp), both in the context of multidrug
                                                                                                                            resistance (mdr) and its physiological roles, also to screen more
                                                                                                                            efficient/ selective Pgp modulators. We are currently focusing on the
                                                                                                                            localization of Pgp within and outside of lipid rafts, in conjunction with its
                                                                                                                            conformational and catalytic characteristics. Our experimental systems
                                                                                                                            include: (A) a novel flow-cytometric method to detect Pgp conformational
                                                                                                                            changes upon its interactions with modulators, based on antibody
                                                                                                                            competition, (B) a flow-cytometric platform for the measurement of raft-,
                                                                                                                            and cytoskeleton-association of cell-surface proteins, applicable for




                                                                                                                                                                                                    35
                                                                                                                         rapid, simple, serial analysis of intermolecular associations between
                                                                                                                         different cell surface proteins, (C) a simple method to completely block
                                                                                                                         Pgp function using an anti-Pgp mAb, (D) a xenotransplantation system
                                                                                                                         in SCID mice to study, by SPECT and PET, the effect of modulators, (E)
                                                                                                                         methods for fluorescence resonance energy transfer (FRET)
                                                                                                                         measurements on cell surface proteins to study inter-, and
                                                                                                                         intramolecular distances. We have flow-cytometers, confocal
                                                                                                                         microscope equipped with FCS, expertise in fluorescence-related
                                                                                                                         methods, with special emphasis on FRET.
Sackler School   Tel Aviv          972-3-      69978   Tel Aviv   Levanon             Israel   fatty acid desaturases,   Our interest is to understand the role of fatty acid metabolism :               scientific
of Medicine-     University        6407842                                                     nutrition,metabolic       synthesis and desaturation specifically, in pathologies leading to the
Minerva Center                                                                                 syndrome                  alteration of glucose and lipid homeostasis.The main emphasis is on
                                                                                                                         nutritional essential fatty acids. We have developed animal and cell
                                                                                                                         culture models of : hypercholesterolemia ,fatty liver and diabetes. We
                                                                                                                         are employing "in vitro" and "in vivo" labelled precursors to follow up lipid
                                                                                                                         synthesis, fatty acid desaturation, cholesterol , bile acid synthesis and
                                                                                                                         lipid composition.We measure the enzymatic pathways involved. We
                                                                                                                         utilize GC, HPLC and TLC methodologies , as well as biochemical and
                                                                                                                         molecular biology determinations. Our purpose is to contribute to the
                                                                                                                         understanding of the impact of nutritional fatty acids on the
                                                                                                                         amelioration of lipid related diseases .
Dept of          Weizmann          ++972-8-    76100   Rehovot    1 Herzl Street      Israel   sphingolipids,            For 15 years. my lab has worked on sphingolipids. We focus on                   scientific
Biological       Institute         9342704                                                     ceramide, glycolipids     sphingolipid synthesis, and also defective degradation in lysosomal
Chemistry                                                                                                                storage diseases. More details can be found at:
                                                                                                                         http://www.weizmann.ac.il/Biological_Chemistry/scientist/futerman/
ISOF/BioFreeRa   Consiglio         ++39-051-   40129   Bologna    Via Piero Gobetti   Italy    trans lipid, radical      Different aspects of the cis-trans isomerization of fatty acid residues by      scientific
dicals           Nazionale delle   639-8309                       101                          stress, isomerization     free radicals are developed: (a) Biomimetic studies using liposomes as
                 Ricerche                                                                                                a model for cell membranes and correlating the isomerization process
                                                                                                                         with possible mechanisms of lipid alteration occurring in vivo. The role of
                                                                                                                         different vitamins. (b) A general approach has been settled for building-
                                                                                                                         up of trans lipid library with application to lipidomic researches.
                                                                                                                         Analytical data on trans lipids and characterization of these lipid
                                                                                                                         components in living organisms, thus contributing to the lipidomic target.
                                                                                                                         This is also related to the examination of biological samples and
                                                                                                                         correlation between trans lipid and radical stress. Endogenous formation
                                                                                                                         of trans lipids and correlation with quality of life and various pathologies.
EPR Lipidomics   Dept Biochemistry ++39 080    70126   BARI       V. E. Orabona, 4    Italy    phospholipid bilayer,     Phospholipid bilayer structure and biochemical/biophysical properties.          scientific
                 and Molecular     5443367                                                     spin labeling EPR,        Spin labeling of phospholipids. EPR studies of spin labelled natural and
                 Biology                                                                       lipoperoxidation,         artificial membranes (mitochondria, erythrocyte ghost, lipid vesicles,
                                                                                               oxidative stress          oriented planar membranes). Lipoperoxidation. Synthesis and
                                                                                                                         purification of oxidized phospholipid species. EPR studies of the effects
                                                                                                                         of oxidized phospholipids in model membranes and in membranes
                                                                                                                         reconstituted with lipidomes from pathological tissues (animal models
                                                                                                                         and humans) and from cultured pathological cell mutants. Mass
                                                                                                                         spectrometry lipidomics of peroxidized phospholipids, mainly lecithins (in
                                                                                                                         collaboration with the local university Chemistry Department).
Istituto         Dept of Cell      ++39-06-    00161   ROME       viale Regina        Italy    isoprostanes,             isoprostanes, prostaglandins, oxidative stress                                  scientific
Superiore di     Biology and       49903087                       Elena, 299                   prostaglandins,




                                                                                                                                                                                                36
Sanità             Neurosciences                                                                       oxidative stress
Anna Maria         Istituto Superiore   ++39 06      00161   Roma          Viale Regina        Italy   sphingolipidoses,        We works on a group of genetic diseases, the sphingolipidoses,              scientific
Vaccaro Unit of    di Sanita            4990 2416                          Elena 299                   saposins,                characterized by the lysosomal storage of sphingolipids due to the
Physiopatology                                                                                         glucosylceramidase,      defective activities of sphingolipid hydrolases or of their physiological
of genetic                                                                                             anionic phospholids+     activators. In particular, we have investigated the alterations in
diseases                                                                                                                        glucosylceramide catabolism in Niemann Pick disease type C. We have
                                                                                                                                shown that the storage of cholesterol in Niemann Pick type C modulates
                                                                                                                                the level and subcellular localization of glucosylceramidase affecting in
                                                                                                                                this way the glucosylceramide degradation (Salvioli et al., J.Biol. Chem.
                                                                                                                                279, 17674-17680, 2004). Moreover, we have shown that the
                                                                                                                                glucosylceramidase N370S mutation, the most common mutation in
                                                                                                                                Gaucher disease, affects the capacity of the enzyme to interact with
                                                                                                                                anionic phospholipid-containing membranes and saposin C, the main
                                                                                                                                physiological activators of glucosylceramidase (Salvioli et al., Biochem.
                                                                                                                                J., 390, 95-105, 2005). Our work is also focused on the study of the
                                                                                                                                mechanism/s of action of saposins and on their interaction with lipids,
                                                                                                                                especially phospholipids. Actually we have contribute to define the
                                                                                                                                mechanism of action of saposin C (Vaccaro et al.,J.Biol.Chem.,272,
                                                                                                                                16862-16867,1997, Salvioli et al., FEBS Letters, 472, 1721, 2000), of
                                                                                                                                saposin D (Ciaffoni et al., J. Biol. Chem., 276, 31583-31589, 2001) and
                                                                                                                                of saposin B (Ciaffoni et al., J. Lip. Res., 47, 1045-1053, 2006).
Department of      Mario Negri Sud      +39          66030   Santa Maria   Via Nazionale 8/A   Italy   phosphoinositides,       Phosphoinositide metabolism and related diseases. Our expertises            scientific
Cell Biology and   Institute            0872570353           Imbaro                                    glycerophosphoinositol   include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid
Oncology                                                                                               s, phospholipases,       binding domain recognition -- PI kinase and phosphatase assays --
                                                                                                       LXR-FXR nuclear          inositol phospate and glycerophosphoinositol analysis by HPLC and MS
                                                                                                       receptors                -- metabolism of the phosphoinositides and their metabolites --
                                                                                                                                Morphological approaches with reference to the LBD (IF, EM,
                                                                                                                                Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and
                                                                                                                                apply the lipidomic approach to the lipid profiling of cell models of
                                                                                                                                different pathological conditions (Overexpressing or KO for
                                                                                                                                proteins/enzymes involved in lipid metabolism and related diseases)
                                                                                                                                Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of
                                                                                                                                the Rho-family cycle: Our studies have indicated that the GPIs, the
                                                                                                                                cellular derivatives of the phosphoinositides, are modulators of actin
                                                                                                                                cytoskeleton assembly through an action on the Rho GTPases (Corda et
                                                                                                                                al., 2002, Mancini et al., 2003). These compounds have thus become
                                                                                                                                the focus of further studies aimed at elucidating their mechanism of
                                                                                                                                action (Mariggiò et al, 2006) and at exploiting them as potential leads for
                                                                                                                                drug development in actin-related diseases (Wiscott-Aldrich syndrome,
                                                                                                                                X-linked mental retardation due to defect in PIX, common variable
                                                                                                                                immunodeficiency). Diseases due to defects in the PI phosphatases:
                                                                                                                                OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy,
                                                                                                                                and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify
                                                                                                                                and validate the enzymes controlling the PI cycle as pharmacological
                                                                                                                                targets, the activities of which can be modulated by inhibitors and/or
                                                                                                                                activators to re-establish the correct balance between the PIs pools in
                                                                                                                                the above-mentioned conditions in which this balance has been
                                                                                                                                compromised by the lack or deregulation of the PIPtases. A similar
                                                                                                                                approach is currently being undertaken in the case of the deregulation of



                                                                                                                                                                                                     37
                                                                                                                                 PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic
                                                                                                                                 diseases, inflammation and diabetes. Other projects involving aspects of
                                                                                                                                 the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism
                                                                                                                                 of membrane fissioning mediated by the protein BARS -Regulation of
                                                                                                                                 lipid metabolism by LXR and FXR nuclear receptor and relevance in the
                                                                                                                                 development of colon cancer
Department of      Mario Negri Sud      +39          66030   Santa Maria   Via Nazionale 8/A   Italy   phosphoinositides,        Phosphoinositide metabolism and related diseases. Our expertises            scientific
Cell Biology and   Institute            0872570353           Imbaro                                    glycerophosphoinositol    include: -- phosphoinositide analysis by standard HPLC, TLC, Lipid
Oncology                                                                                               s, phospholipases,        binding domain recognition -- PI kinase and phosphatase assays --
                                                                                                       LXR-FXR nuclear           inositol phospate and glycerophosphoinositol analysis by HPLC and MS
                                                                                                       receptors                 -- metabolism of the phosphoinositides and their metabolites --
                                                                                                                                 Morphological approaches with reference to the LBD (IF, EM,
                                                                                                                                 Tomography, FRET, FRAP, Correlative light/EM) Our aim is to set and
                                                                                                                                 apply the lipidomic approach to the lipid profiling of cell models of
                                                                                                                                 different pathological conditions (Overexpressing or KO for
                                                                                                                                 proteins/enzymes involved in lipid metabolism and related diseases)
                                                                                                                                 Ongoing projects: The glycerophosphoinositols (GPIs) as modulators of
                                                                                                                                 the Rho-family cycle: Our studies have indicated that the GPIs, the
                                                                                                                                 cellular derivatives of the phosphoinositides, are modulators of actin
                                                                                                                                 cytoskeleton assembly through an action on the Rho GTPases (Corda et
                                                                                                                                 al., 2002, Mancini et al., 2003). These compounds have thus become
                                                                                                                                 the focus of further studies aimed at elucidating their mechanism of
                                                                                                                                 action (Mariggiò et al, 2006) and at exploiting them as potential leads for
                                                                                                                                 drug development in actin-related diseases (Wiscott-Aldrich syndrome,
                                                                                                                                 X-linked mental retardation due to defect in PIX, common variable
                                                                                                                                 immunodeficiency). Diseases due to defects in the PI phosphatases:
                                                                                                                                 OCRL-1, MTM1 and MTMR2 (Lowe Syndrome, Myotubular Myopathy,
                                                                                                                                 and Type 4B Charcot-Marie-Tooth). The aim of our project is to identify
                                                                                                                                 and validate the enzymes controlling the PI cycle as pharmacological
                                                                                                                                 targets, the activities of which can be modulated by inhibitors and/or
                                                                                                                                 activators to re-establish the correct balance between the PIs pools in
                                                                                                                                 the above-mentioned conditions in which this balance has been
                                                                                                                                 compromised by the lack or deregulation of the PIPtases. A similar
                                                                                                                                 approach is currently being undertaken in the case of the deregulation of
                                                                                                                                 PI 3-kinase (PI3K) pathway, which occurs in cancer, thrombotic
                                                                                                                                 diseases, inflammation and diabetes. Other projects involving aspects of
                                                                                                                                 the lipid metabolism are: -role of PLA2 in membrane traffic -mechanism
                                                                                                                                 of membrane fissioning mediated by the protein BARS -Regulation of
                                                                                                                                 lipid metabolism by LXR and FXR nuclear receptor and relevance in the
                                                                                                                                 development of colon cancer
Biochemistry       University Milano-   ++39-02-     20052   Monza         Via Cadore, 48      Italy   Alzheimer, Lipid rafts,   Lipids and neurodegenerative diseases Lipid rafts and disease Lipid         scientific
group              Bicocca              6448-8203                                                      signal transduction       rafts and signal transduction Lipid-protein interaction
Department of      University Milano-   ++39-02-     20052   Monza         Via Cadore, 48      Italy   Alzheimer, Lipid rafts,   Lipids and neurodegenerative diseases Lipid-protein interaction Lipid       scientific
Experimental       Bicocca              6448-8203                                                      signal transduction       rafts and signal transduction Lipid rafts and disease
Medicine
Dipartimento di    University of        ++39055459   50143   Firenze       Viale G.B.          Italy   sphingosine 1-            Sphingosine 1-phosphate and its mechanism of action. Regulation of           scientific
Scienze            Florence             8328                               Morgagni 50                 phosphate, lipid          cellular sphingosine 1-phosphate content. Functional role of membrane
Biochimiche                                                                                            microdomain,              lipid microdomains in sphingosine 1-phosphate production and




                                                                                                                                                                                                      38
                                                                                         ceramide, ceramidase signalling.
Department of     University of   ++39-075-   06100   Perugia   Policlinico      Italy   intranuclear lipid, DNA- We have demonstrated that a phospholipid cellular fraction is associated scientific
Clinical and      Perugia         5729085                       Monteluce, via           lipid, RNA-lipid         with chromatin. The presence, described by histochemical and
Experimental                                                    Brunamonti                                        biochemical techniques, is not due to membrane contaminations. The
Medicine,                                                                                                         chromatin phospholipid fraction (CPF) can be considered a chromatin
Physiopathology                                                                                                   minor component. The CPF differ from that of microsomes and nuclear
, University                                                                                                      membranes as regard composition and turnover and changes in relation
School of                                                                                                         to hepatocyte maturation and proliferation. The main modifications,
Medicine                                                                                                          evident in several cellular functions, regard principally
                                                                                                                  phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised
                                                                                                                  directly in the nuclei in a very short time with a fine modulation of the
                                                                                                                  enzimatic activities, regulated by PLs. The presence of a complex of
                                                                                                                  base exchange enzymes for PC synthesis, a neutral sphingomyelinase
                                                                                                                  (N-SMase), a sphingomyelin-synthase (SM-synthase), a
                                                                                                                  phosphatidylcholine-dependent phospholipase C (PC-PLC) and a
                                                                                                                  sphingomyelin-synthase-reverse (SM-synthase-reverse), which
                                                                                                                  synthesises PC from SM have been demonstrated. These enzymes
                                                                                                                  differ for pH and Km optima from those present in nuclear membranes,
                                                                                                                  suggesting the possible existence of different isoforms. These results
                                                                                                                  strongly support the presence of a metabolic machinery in the chromatin
                                                                                                                  which probably is regulated in relation to cellular function. The PC-PLC
                                                                                                                  and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG),
                                                                                                                  whereas the N-SMase and SM-synthase-reverse enrich the intranuclear
                                                                                                                  pool of ceramide. DAG and ceramide are know as second messengers
                                                                                                                  which play an important role in different physiological conditions, acting
                                                                                                                  as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase
                                                                                                                  C (PKC) The hypothesis has been made that the lipid messenger can
                                                                                                                  favour the nuclear molecular events which precede DNA synthesis in
                                                                                                                  liver regeneration. The results show that the DAG pool increases at the
                                                                                                                  beginning of S phase, whereas the ceramide pool later increases. In the
                                                                                                                  erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-
                                                                                                                  SMase activity increases during the apoptotic process, whereas PC-PLC
                                                                                                                  activity increases during cell differentiation. It will be interesting to know
                                                                                                                  if DAG and ceramide, produced directly in chromatin, can play a role in
                                                                                                                  cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R.
                                                                                                                  and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in
                                                                                                                  hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2.
                                                                                                                  .Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F.
                                                                                                                  "Phospholipids in chromatin: incorporation of 32PO24 in different
                                                                                                                  subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-
                                                                                                                  288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce
                                                                                                                  R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl.
                                                                                                                  Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P.
                                                                                                                  and Gahan P.B. "Age-related changes in chromatin phospholipid
                                                                                                                  fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989.
                                                                                                                  5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in
                                                                                                                  phospholipid composition of rat liver cell nuclei and nuclear envelopes".
                                                                                                                  Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6.
                                                                                                                  Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin



                                                                                                                                                                                     39
phospholipid changes during rat liver development" Cell Biochem. and
Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo
dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e
Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni
M.P. "Tem cytochemical study of the localization of phospholipids in
interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235,
1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-
Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719-
1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids
and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E.,
Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte
nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F.
1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid
composition on rat liver nuclear membrane fluidity" Cell Biochem. and
Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P.
"Chromatin neutral spingomyelinase and its role in hepatic regeneration.
Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14.
Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear
sphingomyelin protects RNA from RNase action" FEBS Letters 431,
443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola
Magni,M.P. Changes of nuclear membrane fluidity during rat liver
regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16.
Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane
Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy
Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17.
Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear
Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609
18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent
Phospholipase C in Rat Liver Chromatin
Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19.
Albi,E., Viola Magni,M.P. The presence and the role of chromatin
cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400,
2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P.,
Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new
immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21.
Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol
antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm.
2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola
Magni,M.P. A possible role of cholesterol-
sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver
regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E.,
Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin
changes in cell proliferation and/or apoptosis induced by ciprofibrate. J.
Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla
Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai,
Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies
In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F.
2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S.,
Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-



                                                                      40
                                                                                                                  dependent Phospholipases C in cell cycle progression during rat liver J.
                                                                                                                  Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo
                                                                                                                  Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in
                                                                                                                  Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609
                                                                                                                  27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia
                                                                                                                  Sartori Plasmalogens in rat liver chromatin: new molecules involved in
                                                                                                                  cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28.
                                                                                                                  Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni,
                                                                                                                  Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica
                                                                                                                  Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in
                                                                                                                  serum deprivation-induced apoptosis of embryonic hippocampal cells J
                                                                                                                  Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta ,
                                                                                                                  Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase
                                                                                                                  and protein kinase C delta in melanoma cells Arch. Biochem Biophys,
                                                                                                                  2005, 438:156-61. I.F. 2.338
Department of     University of   ++39-075-   06100   Perugia   Policlinico      Italy   intranuclear lipid, DNA- We have demonstrated that a phospholipid cellular fraction is associated scientific
Clinical and      Perugia         5729085                       Monteluce, via           lipid, RNA-lipid         with chromatin. The presence, described by histochemical and
Experimental                                                    Brunamonti                                        biochemical techniques, is not due to membrane contaminations. The
Medicine,                                                                                                         chromatin phospholipid fraction (CPF) can be considered a chromatin
Physiopathology                                                                                                   minor component. The CPF differ from that of microsomes and nuclear
, University                                                                                                      membranes as regard composition and turnover and changes in relation
School of                                                                                                         to hepatocyte maturation and proliferation. The main modifications,
Medicine                                                                                                          evident in several cellular functions, regard principally
                                                                                                                  phosphatidylcholine (PC) and sphingomyelin (SM) that are metabolised
                                                                                                                  directly in the nuclei in a very short time with a fine modulation of the
                                                                                                                  enzimatic activities, regulated by PLs. The presence of a complex of
                                                                                                                  base exchange enzymes for PC synthesis, a neutral sphingomyelinase
                                                                                                                  (N-SMase), a sphingomyelin-synthase (SM-synthase), a
                                                                                                                  phosphatidylcholine-dependent phospholipase C (PC-PLC) and a
                                                                                                                  sphingomyelin-synthase-reverse (SM-synthase-reverse), which
                                                                                                                  synthesises PC from SM have been demonstrated. These enzymes
                                                                                                                  differ for pH and Km optima from those present in nuclear membranes,
                                                                                                                  suggesting the possible existence of different isoforms. These results
                                                                                                                  strongly support the presence of a metabolic machinery in the chromatin
                                                                                                                  which probably is regulated in relation to cellular function. The PC-PLC
                                                                                                                  and SM-synthase enrich the intranuclear pool of diacylglicerol (DAG),
                                                                                                                  whereas the N-SMase and SM-synthase-reverse enrich the intranuclear
                                                                                                                  pool of ceramide. DAG and ceramide are know as second messengers
                                                                                                                  which play an important role in different physiological conditions, acting
                                                                                                                  as a stimulating (DAG) and inhibitory factor (ceramide) of protein kinase
                                                                                                                  C (PKC) The hypothesis has been made that the lipid messenger can
                                                                                                                  favour the nuclear molecular events which precede DNA synthesis in
                                                                                                                  liver regeneration. The results show that the DAG pool increases at the
                                                                                                                  beginning of S phase, whereas the ceramide pool later increases. In the
                                                                                                                  erythroleukemic cells, treated with DMSO and/or D3 vitamin, the N-
                                                                                                                  SMase activity increases during the apoptotic process, whereas PC-PLC
                                                                                                                  activity increases during cell differentiation. It will be interesting to know
                                                                                                                  if DAG and ceramide, produced directly in chromatin, can play a role in
                                                                                                                  cellular functions. 1. Viola Magni M.P., Gahan P.B., Albi E., Iapoce R.



                                                                                                                                                                                     41
and Gentilucci P.F. "Chromatin phospholipids and DNA synthesis in
hepatic cells". Bas. Appl. Histochem. 29, 253-259, 1985. I.F. 1.041 2.
.Viola Magni M.P., Gahan P.B., Albi E., Iapoce R. and Gentilucci P.F.
"Phospholipids in chromatin: incorporation of 32PO24 in different
subcellular fraction of hepatocytes" Cell Biochem. and Function 4, 283-
288, 1986. I.F. 1.452 3. Viola Magni M.P., Gahan P.B., Albi E., Iapoce
R. and Gentilucci P.F. "Synthesis of chromatin phospholipids" Bas. Appl.
Histochem. 31, 355-364, 1987. I.F. 1.041 4. Albi E., Viola Magni M.P.
and Gahan P.B. "Age-related changes in chromatin phospholipid
fraction" The liver, metabolism and ageing, Eurage 13,189-188, 1989.
5. Gahan P.B., Albi E. and Viola Magni M.P. "Changes with age in
phospholipid composition of rat liver cell nuclei and nuclear envelopes".
Drug metabolism, liver injury and ageing, Eurage, 16, 229-236, 1991. 6.
Albi E., Viola Magni M.P., Lazzarini R. and Gahan P.B. "Chromatin
phospholipid changes during rat liver development" Cell Biochem. and
Function 9, 119-123, 1991. 1.452. I.F. 1.452 7. Albi E. "Presenza e ruolo
dei fosfolipidi nella cromatina" Tesi di Dottorato in Patologia Cellulare e
Molecolare. 1991. 8. Fraschini A., Albi E., Gahan P.B. and Viola Magni
M.P. "Tem cytochemical study of the localization of phospholipids in
interphase chromatin in rat hepatocytes" Histochemistry 97, 225-235,
1992. I.F 9. Albi E. , Mersel M., Leray C., Tomassoni M.L. and Viola-
Magni M.P. Rat Liver Chromatin Phospholipids" Lipids 29, 715-719-
1994. I.F. 2.164 10. Albi E., Micheli M., Viola-Magni M.P. "Phospholipids
and nuclear RNA" Cell Biol. Intern. 20, 6, 1996. I.F. 1.092 11. Albi E.,
Viola-Magni M.P. "Choline base exchange activity in rat hepatocyte
nuclei and nuclear membrane" Cell Biol.Intern 21, 217-221, 1997. I.F.
1.092 12. Albi E., Tomassoni M.L., Viola-Magni M.P. "Effect of lipid
composition on rat liver nuclear membrane fluidity" Cell Biochem. and
Funct, 15, 181-190, 1997. I.F. 1.452 13. Albi, E. and Viola Magni, M.P.
"Chromatin neutral spingomyelinase and its role in hepatic regeneration.
Biochim. Biophys. Res. Commun., 236, 29-33, 1997. I.F. 2.836 14.
Micheli,M., Albi,E, Leray,C., and Viola Magni,M.P. "Nuclear
sphingomyelin protects RNA from RNase action" FEBS Letters 431,
443-447, 1998. I.F. 3.609 15. Tomassoni,M.L., Albi,E., and Viola
Magni,M.P. Changes of nuclear membrane fluidity during rat liver
regeneration Biochem. Mol. Biol. Intern. 47, 1049-1059, 1999. 16.
Albi,E., Peloso,I., and Viola Magni,M.P. Nuclear Membrane
Sphingomyelin-Cholesterol Changes in Rat Liver after Hepatectomy
Biochem. Biophys. Res. Commun. 262, 692-695, 1999. I.F. 2.836 17.
Albi,E., Viola Magni,M.P. Sphingomyelin-Synthase in Rat Liver Nuclear
Membrane and Chromatin FEBS Letters 460, 369-372, 1999. I.F. 3.609
18. Albi,E., and Viola Magni,M.P. Phosphatidylcholine-Dependent
Phospholipase C in Rat Liver Chromatin
Biochem.Biophys.Res.Commun. 265, 640-643, 1999. I.F. 2.836 19.
Albi,E., Viola Magni,M.P. The presence and the role of chromatin
cholesterol in rat liver regeneration Journal of Hepatology 36, 395-400,
2002. I.F. 5.285 20. Caso,V., Panarelli,P., Albi,E., Viola-Magni,M.P.,
Parnetti,L., Gallai,V. Phospholipid autoantibodies: time for a new
immuno-assay? Clin Exp Hypertens. 24: 511-516, 2002. I.F. 0.816 21.



                                                                      42
                                                                                                                 Panarelli,P., Viola-Magni,M.P., and Albi E. Antiphosphatidylinositol
                                                                                                                 antibody in deep venous thrombosis patients Int.J. Immunopath. Pharm.
                                                                                                                 2003, 16: 61-6. I.F. 3.927 22. Albi,E., Cataldi,S., Rossi,G., and Viola
                                                                                                                 Magni,M.P. A possible role of cholesterol-
                                                                                                                 sphingomyelin/phosphatidylcholine in nuclear matrix during rat liver
                                                                                                                 regeneration. J. Hepatology 2003, 38: 623-8. I.F. 5.283 23. Albi,E.,
                                                                                                                 Pieroni,S., Viola Magni,M.P., and Sartori,C Chromatin sphingomyelin
                                                                                                                 changes in cell proliferation and/or apoptosis induced by ciprofibrate. J.
                                                                                                                 Cell Physiol. 2003, 196:354-61. I.F. 5.436 24. Valeria Caso, Lucilla
                                                                                                                 Parnetti, Paolo Panarelli, Maria Pia Viola Magni, MD, Virgilio Gallai,
                                                                                                                 Elisabetta Albi Selection of Thrombogenetic Antiphospholipid Antibodies
                                                                                                                 In Cerebrovascular Disease Patients J.Neurol 2003, 250: 593-597. I.F.
                                                                                                                 2.778 25. Albi,E., Rossi,G., Maraldi,N.M., Viola Magni,M.P., Cataldi,S.,
                                                                                                                 Solimando, L., Zini,N. Involvement of nuclear Phosphatidylinositol-
                                                                                                                 dependent Phospholipases C in cell cycle progression during rat liver J.
                                                                                                                 Cell Physiol. 2003, 197: 181-188. I.F. 5.463 26. Elisabetta Albi, Remo
                                                                                                                 Lazzarini and Mariapia Viola Magni Reverse Sphingomyelin-Synthase in
                                                                                                                 Rat Liver Chromatin FEBS Letters 2003, 549(1-3):152-156. I.F. 3.609
                                                                                                                 27. Elisabetta Albi, Samuela Cataldi, Mariapia Viola Magni and Claudia
                                                                                                                 Sartori Plasmalogens in rat liver chromatin: new molecules involved in
                                                                                                                 cell proliferation J. Cell Physiol, 2004, 201(3):439-46. I.F. 5.463 28.
                                                                                                                 Elisabetta Albi, Samuela Cataldi, Elisa Bartoccini, Mariapia Viola Magni,
                                                                                                                 Francesca Marini, Francesca Mazzoni, Giuseppe Rainaldi, Monica
                                                                                                                 Evangelisti, Mercedes Garcia-Gil Nuclear sphingomyelin pathway in
                                                                                                                 serum deprivation-induced apoptosis of embryonic hippocampal cells J
                                                                                                                 Cell Physiol, 2005. I.F. 5.463 29. Elisabetta Albi, Caterina AM La Porta ,
                                                                                                                 Samuela Cataldi, Mariapia Viola Magni Nuclear sphingomyelin-synthase
                                                                                                                 and protein kinase C delta in melanoma cells Arch. Biochem Biophys,
                                                                                                                 2005, 438:156-61. I.F. 2.338
Department of   University of   0039 75   06122   Perugia   Via del Giochetto   Italy   pollen phospholipids,    I)Plant pollens are an important source of environmental antigens that      scientific
Internal        Perugia         5857423                                                 brain mitochondria,      stimulate allergic responses. In addition to acting as vehicled for foreign
Medicine                                                                                anionic phospholipids,   protein antigens, they contain different molecular species of lipids, which
                                                                                        cardiolipin              are necessary in the reproduction of higher plants. The CD1 family of
                                                                                                                 nonpolymorphic major histocompatibility complex-related molecules is
                                                                                                                 highly conserved in mammals, and has been shown to present microbial
                                                                                                                 and self lipids to T cells. We provided evidence that pollen lipids may be
                                                                                                                 recognized as antigens by human T cell through a CD1-dependent
                                                                                                                 pathway. Amonng phospholipids extracted from cypress grains,
                                                                                                                 phosphatidylcholine (PC)and phosphatidylethanolamine (PE)were able
                                                                                                                 to stimulate the proliferation of T cells from cypresse-sensitive subjects.
                                                                                                                 Particularly, 18:2/18:2 PC, a predominat component of cypress PC,
                                                                                                                 could be one of the specific targets in pollen grain capture and
                                                                                                                 recognition by CD1. Also pollen-derived unsaturated PE are antigens
                                                                                                                 recognized by a variety of T clones derived from allergic subjects. The
                                                                                                                 nature of the acyl chains in the lipid antigens contributes enormously to
                                                                                                                 immunogenicity.(Agea et al. Journal Exp. Med. (2005) 202:295-308. The
                                                                                                                 nature of lipid-protein interaction between phospholipids and CD1 is a
                                                                                                                 target for future investigation. The antigenic properties of glycolipids
                                                                                                                 extracted and purified from pollens will be also evaluated. II) We are



                                                                                                                                                                                      43
                                                                                                                    also involved in projects aimed at studying the role of anionic
                                                                                                                    phospholipids, particularly cardiolipin, in the functionality of mitochondria
                                                                                                                    in the nervous tissue. A major finding of our previous studies was the
                                                                                                                    effect exerted by exogenous phospholipids on the release of cytochrome
                                                                                                                    c from the inner mitochondrial membrane and on the transmembrane
                                                                                                                    potential of brain mitochondria. Mitochondria were enriched with
                                                                                                                    exogenous phosoholipids through a fusion process in the outer
                                                                                                                    mitochondrial membrane. The experimental system utilized a fluorescent
                                                                                                                    probe inserted in the outer mitochondrial membrane to follow the
                                                                                                                    kinetics of incorporation of lipids in mitochondria. By using a selective
                                                                                                                    probe for cardiolipin, it has been demonstrated thet the fused lipid can
                                                                                                                    reach the inner mitochondrial membrane and influence the membrane
                                                                                                                    potential. We found that exogenous cardiolipin reinforced the binding of
                                                                                                                    cytochrome c with the inner mitochondria, thus hindering its release
                                                                                                                    following different stimuli. The interaction of cytochrome c with the
                                                                                                                    components of the inner mitochondrial membrane is object of
                                                                                                                    investigation. The mode of membrane association of cytochrome c is
                                                                                                                    studied by using a model system of cytochrome c reconstituted in
                                                                                                                    different cardiolipins. (Piccotti et al. JBC 2002, 277:12075-12081,
                                                                                                                    Piccotti et al. J. Membr. Biol. 2004, 198:43-53)
Department of   University of   ++39-075-   I-06122   Perugia   Via del Giochetto   Italy   phospholipase A2, lipid This group is interested in the identification and localization of various    scientific
Internal        Perugia         585-7420                                                    mediators, PAF          phospholipases A2 in intracellular compartments. Particular attention is
Medicine,                                                                                                           devoted to secretory PLA2s with the aim of uderstanding their functions
Section of                                                                                                          in normal and pathological conditions. We have already demonstrated
Biochemistry                                                                                                        the presence of a group IIA sPLA2 from cerebral cortex mitochondria
                                                                                                                    and its release under energy-deficient conditions and the location of
                                                                                                                    group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome
                                                                                                                    cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279,
                                                                                                                    36 (3), 37860-37869). Furthermore we have set up a procedure for a
                                                                                                                    continuous monitoring PLA2 activities in cultured cells in vivo or
                                                                                                                    subcellular organelles. Since many cell types release low molecular
                                                                                                                    weight sPLA2, we are also studying the routing for their secretion of their
                                                                                                                    translocation to intracellular compartments. This group is also
                                                                                                                    interested on the formation of lipid mediators and particularly of PAF.
                                                                                                                    We have identified the presence of the enzymes for its synthesis in the
                                                                                                                    nervous tissue.
Department of   University of   ++39-075-   I-06122   Perugia   Via del Giochetto   Italy   phospholipase A2, lipid This group is interested in the identification and localization of various    scientific
Internal        Perugia         585-7420                                                    mediators, PAF          phospholipases A2 in intracellular compartments. Particular attention is
Medicine,                                                                                                           devoted to secretory PLA2s with the aim of uderstanding their functions
Section of                                                                                                          in normal and pathological conditions. We have already demonstrated
Biochemistry                                                                                                        the presence of a group IIA sPLA2 from cerebral cortex mitochondria
                                                                                                                    and its release under energy-deficient conditions and the location of
                                                                                                                    group V sPLA2 in the nuclei of cultured PC-12 and U251 astrocytome
                                                                                                                    cells of group V sPLA2 (Macchioni et al., J Biol Chem. (2004) Vol. 279,
                                                                                                                    36 (3), 37860-37869). Furthermore we have set up a procedure for a
                                                                                                                    continuous monitoring PLA2 activities in cultured cells in vivo or
                                                                                                                    subcellular organelles. Since many cell types release low molecular
                                                                                                                    weight sPLA2, we are also studying the routing for their secretion of their
                                                                                                                    translocation to intracellular compartments. This group is also



                                                                                                                                                                                           44
                                                                                                                                  interested on the formation of lipid mediators and particularly of PAF.
                                                                                                                                  We have identified the presence of the enzymes for its synthesis in the
                                                                                                                                  nervous tissue.
Funato Group       Hiroshima             ++81-82-     739-   Higashi-    1-4-4,              Japan       ceramide, GPI,           lipid trafficking                                                              scientific
                   University            424-7925     8528   Hiroshima   Kagamiyama                      transport
Department of      Osaka University      ++81-6-      662-   Suita       2-2 Yamadaoka       Japan       HPLC/MS,                 I has developed an HPLC/ESI/MS-based method capable of analyzing a scientific
Biochemistry       Graduate School       6879-3283    0018                                               sphingolipids,           wider range of lipid classes from neutral lipids to phospholipids on a
and Molecular      of Medicine                                                                           phospholipids            single chromatographic run with three solvent gradients and post-
Biology                                                                                                                           column mixing of solvent that helps ESI of lipids eluted earlier with non-
                                                                                                                                  polar solvents. We used this method to study on lipid anomaly of a
                                                                                                                                  variety of gene-targeted mice tissues and subcellular organellae [e.g.,
                                                                                                                                  Takagi, S., Tojo, H., Tomita, S., Sano, S., Itami, S., Hara, M., Inoue, S.,
                                                                                                                                  Horie, K., Kondoh, G., Hosokawa, K., Gonzalez, F.J. and Takeda, J.
                                                                                                                                  Alteration of the 4-sphingenine scaffolds of ceramides in keratinocyte-
                                                                                                                                  specific Arnt deficient mice affects skin barrier function. J. Clin. Invest.
                                                                                                                                  112, 1372-1382 (2003)]. We are now extending the method amenable to
                                                                                                                                  analyzing more polar lipids that cantain important bioactive lipids. We
                                                                                                                                  eventually would like to develop a very comprehensive lipid analyzing
                                                                                                                                  system.
Department of      The University        81-3-5841-   113-   Tokyo       7-3-1, Hongo,       Japan       lipid, database          create lipid database "LipidBank"                                            scientific
Metabolome,        ofTokyo               3651         0033               Bunkyo-ku
Graduate School
of Medicine
Laboratory         Academic Medical +31-20-           NL-    Amsterdam   P.O. Box 22660      Netherlan   tandem-Mass              HPLC-tandem mass spectrometric analysis of lipid compositions at              scientific
Genetic            Center           5669111           1100                                   ds          Spectrometry,            different levels of complexity, such as cell compartments/organelles,
Metabolic                                             DD                                                 glycerophospholipids,    cells, tissues in order to clarify/study biochemical processes in relation to
Diseases                                                                                                 cardiolipin, ceramides   disease based disorders. Research is biomedical orientated in a
                                                                                                                                  university hospital setting and findings are where possible translated into
                                                                                                                                  diagnostic tests.
DSM                analysis/spectrom     0031         2600   Delft       p.o. box 1          Netherlan   ceramides,               ceramides, sphingolipids                                                      industrial
                   etry                  152792278    MA                                     ds          sphingolipids
Lipid laboratory   Dept Internal         -            6500   Nijmegen    Geert Grooteplein   Netherlan   GC-MS, steroids,         lipidperoxydation and antioxidants, lipid transfer, steroids, nutrients        scientific
                   Medicine, UMC                      HB                 zuid 8              ds          lipoproteins
                   Nijmegen
Physics of Life    Leiden Institute of   +31 71 527   2333   Leiden      Niels Bohrweg 2     Netherlan   cell signaling, model    cell signaling, model systems, lipid rafts, single-molecule biophysics,        scientific
Processes          Physics, Leiden       5982         CA                                     ds          systems, lipid rafts,    fluorescence microscopy
                   University                                                                            single-molecule
                                                                                                         biophysics,
                                                                                                         fluorescence
                                                                                                         microscopy
NIOZ               NIOZ                  31 222       1790   Den Burg    P.O. Box 59         Netherlan   organic geochemistry,    As part of the ICOMM (http://icomm.mbl.edu/)work we are building up a          scientific
                                         369582       AB                                     ds          biomarker                lipid database. If possible, we want to combine our data with yours.
                                                                                                                                  Firstly, we are interested in a procedure to find data from the database.
                                                                                                                                  What programs are developed, how do they work. If there is no such
                                                                                                                                  program exists, one has to be created. We hope we can work with you
                                                                                                                                  to get things working.
Philips            Philips               +31-40-      5656   Eindhoven   High Tech           Netherlan   cholsterol, membrane     Bioactive lipids relevant to diseases                                          industrial
Research,                                2743794      AE                 Campus 4            ds          microdomains,




                                                                                                                                                                                                            45
Molecular                                                                                               sphingolipids, oxidized
Diagnostics                                                                                             lipids, lipoproteins
Bijvoet Center      Utrecht University   ++31-30-    NL-584   Utrecht      Padualaan 8      Netherlan   membrane membrane-           Lipids is membranes and as signalling entities                                  scientific
                                         2533498     CH                                     ds          protein rafts
Institute of        Utrecht University   +31-30-     3584     Utrecht      Padualaan 8      Netherlan   lipid cell biology - lipid   The Institute of Biomembranes is an interdisciplinary research institute        scientific
Biomembranes                             2536616     CH                                     ds          mass spectrometry -          and graduate school at Utrecht University, accommodating 18 research
                                                                                                        model membrane               groups from the faculties of Science, Medicine, and Veterinary Medicine.
                                                                                                        biochemistry                 The famous lipid biochemist Laurens van Deenen was one of the
                                                                                                                                     founding members of the institute in 1991, but unfortunately deceased in
                                                                                                                                     1994. To remember him, since 2004 the Institute annually awards the
                                                                                                                                     "van Deenen Medal" to a leading active scientist in biomembrane
                                                                                                                                     research (http://ib.bio.uu.nl/). The present director chairs a specific
                                                                                                                                     support action of the European Commission entitled "The European
                                                                                                                                     Lipidomics Initiative" (www.lipidomics.net). A number of groups in the
                                                                                                                                     institute work on questions related to the structure and function of lipids
                                                                                                                                     in cells, blood (lipoproteins) and model membranes. Notably, a
                                                                                                                                     dedicated effort in lipid mass spectrometry is ongoing in the Dept. of
                                                                                                                                     Biochemistry and Cell Biology, Faculty of Veterinary Medicine
                                                                                                                                     (http://www.vet.uu.nl/bc).
Nutrition,          Wageningen           ++31-317-   6703     Wageningen   Bomenweg 2       Netherlan   transcriptomics,             Our group is interested in regulation of gene expression by fatty acids.        scientific
metabolism and      University           485787      HD                                     ds          PPARs, fatty acids           We use transcriptomics (in house affymetrix platform) in combination
genomics group                                                                                                                       with knock-out mice models to investigate the overall impact as well as
                                                                                                                                     the mechanism of fatty acid-dependent gene regulation in numerous
                                                                                                                                     organs (small intestine, liver, heart). Our expertise is: 1) design, analysis
                                                                                                                                     and interpretation of transcriptomics experiments, and 2) pathway
                                                                                                                                     mapping of transcriptomics data with special emphasis on lipid
                                                                                                                                     metabolism
Preclinical         GE Healthcare        +47 2318    0401     Oslo         Nycoveien 2      Norway      Phospholipids, blood,        Phospholipid analysis related to contrast agents for medical imaging.           industrial
sciences                                 5666                                                           drug development             Analyses of blood samples in order to describe
                                                                                                                                     pharmacokinetics/toxicokinetics.
Inst. for Cancer    The Norwegian        47 22934294 0310     Oslo         Montebello       Norway      glycosphingolipids,          Studies of the role of different lipids on intracellular transport of protein   scientific
Research/Sandv      Radium Hospital                                                                     toxins, cholesterol,         toxins. For recent publications, please see the home
igs                                                                                                     rafts                        pages:http://radium.no/sandvig/ We have been studying the role of
group:Intracellul                                                                                                                    cholesterol in transport, and we have been investigating cells with
ar transport                                                                                                                         mutations in (glyco)sphingolipid synthesis. Studies on the roles of
                                                                                                                                     glycospingolipids are in progress.
Laboratory of       Centre for           ++48-42-    93-232   Lodz         Lodowa 106       Poland      ABC transporters,            Lipid hormones and second messengers - their direct and indirect                scientific
Transcriptional     Medical Biology      2723639                                                        gene expression,             effects on gene expression. Lipid-binding transcription factors. Lipid
Regulation          PAS                                                                                 transcription factors        transporters from the ABC superfamily - mechanism of action, regulation
                                                                                                                                     of expression.
Department of       Faculty of           00-40-21-   050095   Bucharest    Splaiul          Romania     Plane lipid bilayers,        Our group is interested to test antidepressants (natural and synthetic)         scientific
Animal              Biology,             318 15 69                         Independentei,               Liposomes,                   and neuroleptics on plane lipid bilayers by electrical recordings and
Physiology and      University of                                          91-95                        Pharmacology                 liposomes by fluorescence polarisation.
Biophysics          Bucharest
Institute of        University of        -386 1      1000     Ljubljana    Lipiceva 2       Slovenia    vesicle shapes,              Mechanical properties of lipid membranes. Theoretical and experimental          scientific
Biophysics,         Ljubljana            5437600                                                        membrane trafficking,        studies of phospholipid vesicle shape behavior. Shape induced
Faculty of                                                                                              amphitropic proteins         mechanisms for the lateral segregation of membrane components. Role
Medicine                                                                                                                             of vesicle shapes in intracellular membrane trafficking. Mechanism of




                                                                                                                                                                                                             46
                                                                                                                             action of amphitropic proteins studied by vesicle shape transformations
                                                                                                                             induced by their binding to vesicle membranes.
INSTITUTO DE       CONSEJO             0034958181   18100   ARMILLA-     Parque             Spain   Parasites, lipid         Lipid translocation in arasites of health interest: their implications in the   scientific
PARASITOLOGI       SUPERIOR DE         667                  GRANADA      Tecnológico de             translocation,           biology of parasites, and their potential use as drug targets.
AY                 INVESTIGACION                                         Ciencias de la             Aminophospholipid        Aminophospholipid translocases and ABC transporters in parasites: its
BIOMEDICINA        ES CIENTÍFICAS                                        Salud. Avda. del           translocases, ABC        role in drug resistance and infectivity of parasites.
LOPEZ-NEYRA,                                                             Conocimiento s             transporters.
Working Group:
BIOCHEMISTR
Y AND
MOLECULAR
PHARMACOLO
GY. Dr.
FRANCISCO
GAMARRO
IQAC/Research      CSIC                34-93-       08034   Barcelona    Jordi Girona 18    Spain   combinatorial            Drug discovery and pharmacological tools: Effects of combinatorial              scientific
Unit on                                4006115                                                      chemistry, nuclear       libraries of synthetic chemicals on cell sphingolipid profiles. Toxicity of
BioActive                                                                                           magnetic ressonance,     environmental contaminants: Deciphering whether the toxicity of
Molecules                                                                                           drug discovery,          selected environmental contaminants occurs by altering the sphingolipid
                                                                                                    environmental            composition of affected cells. Sphingolipid maps as sensors of
                                                                                                    contaminants             environmental contamination. Analytical methodology: (1) Development
                                                                                                                             of 15N and 31P NMR procedures to construct sphingolipid and
                                                                                                                             phospholipid maps. (2) Application of biocompatible chemical reactions
                                                                                                                             to the construction of pseudolipid maps and validation of this approach
                                                                                                                             to research in cell biology.
Unidad de          CSIC and            ++34-94-     48940   Leioa        Sarriena s         Spain   membrane lipids, lipid   We are interested in the biophysics of membrane lipids, and lipid-protein       scientific
Biofísica          Universidad del     601-2625                                                     biophysics, membrane     interactions, with an emphasis on sphingolipids, lipid signalling,
                   País Vasco                                                                       domains, lipid-protein   membrane domains, and, more recently, lipoproteins. Our techniques
                                                                                                    interaction              include calorimetry (DSC, ITC), spectroscopy (IR, fluorescence, UV-vis,
                                                                                                                             stopped-flow, CD), confocal microscopy, Langmuir balance, and
                                                                                                                             supported membranes.
National Center    Spanish             ++34-91-     28049   Madrid       Darwin, 3.         Spain   signaling, chemotaxis,   The previous work of my group has been focused in understand how                scientific
of Biotechnology   Research Council    5854840                           Campus                     leukocyte, HIV           lipid rafts organize cell signaling during leukocyte chemotaxis as well as
                                                                         Cantoblanco                                         in the pivotal function of these microdomains at the early steps of the
                                                                         University                                          human immunodeficiency virus infection.
Institute of       Spanish             +34-983-     E-      Valladolid   Calle Sanz y       Spain   Phospholipase A2,        Eicosanoids, Lysophospholipids, Glycerophospholipids                            scientific
Molecular          Research Council    423-062      47003                Fores s                    Arachidonic Acid,
Biology and        & University of                                                                  Prostaglandins,
Genetics           Valladolid School                                                                Lysophosphatidylcholin
                   of Medicine                                                                      e
BIOMIL             Universidad         ++34-91-     28040   Madrid       Fac. Biologia,     Spain   pulmonary surfactant,      Membrane lateral structure and its role in the establishment of lipid-        scientific
(BIOphysics of     Complutense         3944994                           Dept. Bioquimica           monolayers,                protein and protein-protein interactions. Lipids and lipid/protein
Membranes and                                                                                       membrane domains,          complexes as surface-active agents, with particular emphasis in the
Lipid/Protein                                                                                       surface activity           structure and molecular mechanisms of membranes and surface layers
Interfaces)                                                                                                                    of natural pulmonary surfactant and clinical preparations used in the
                                                                                                                               therapeutical treatment of respiratory diseases.
Research Group     University of       ++34-        E-      Murcia       Facultad de        Spain   lipid-protein interaction, Protein-lipid interactions: interactions with model membranes of              scientific
in                 Murcia              968364766    30100                Veterinaria,               PKCs, diacylglycerol,      signalling proteins, such as PKCs and Bcl-2 family of proteins




                                                                                                                                                                                                      47
Biomembranes,                                                            Campus de                        liposomes                  associated to the regulation of cell apoptosis. Lipid regulation of the
Department of                                                            Espinardo                                                   activity of PKCs. The action of a variety of bioactive lipìds on membrane
Biochemistry                                                                                                                         translocation and activation of the Protein Kinase C family of proteins
and Molecular                                                                                                                        associated to cell signalling. The action of lipids such as
Biology                                                                                                                              phosphatidylserine, phosphoinositides, free fatty acids, diacylglycerols
                                                                                                                                     and ceramides is invetigated. Techniques used: NMR (CPMAS,
                                                                                                                                     HRMAS, deuterium NMR, 31P-NMR), DSC, ITC, Biacore, Fluorescence
                                                                                                                                     spectroscopy, confocal microscopy, X-ray diffraction.
Dept.            University of the   ++34946012       48940   Bilbao     Sarriena            Spain        Cholesterol, Liver,        Disorders of lipid and lipoprotein metabolism involved in hepatic and        scientific
Physiology,      Basque Country      846                                                                  VLDL secretion,            cardiovascular diseases. Molecular mechanisms underlying
Medical School                                                                                            functional genomics        nonalcoholic fatty liver disease. Functional genomics in nonalcoholic
                                                                                                                                     fatty liver disease.
The Wenner-      Stockholm           ++46-8-          SE1069 Stockholm   Arrhenius lab. F3   Sweden       fatty acid metabolism,     Our topic is the regulation of very long chain fatty acid syntersis in       scientific
Gren Institute   University          164127           1                                                   elongase, gene             mammals and their significance in lipid metabolism.
                                                                                                          expression
Department of    Swedish             please fill in   23053   Alnarp     Box 44              Sweden       plant oil biosynthesis     please fill in                                                               scientific
Crop Science     University of
                 Agricultural
                 Sciences
Department of    Umeå University     +469078652       SE-     Umeå       Linaeus väg 10      Sweden       domains, lateral           Lipid/protein interaction, regulation of lipid composition in cell           scientific
Biophysical                          28               90187                                               diffusion, Lipid/protein   membranes, Formation of domains in lipid membranes, Lipid lateral
Chemistry                                                                                                                            diffusion, Molecular ordering in membranes,.
LC-MS Support    Applera Europe      +41 41 799       6343    Rotkreuz   Grundstrasse 10     Switzerlan   phospholipid,              Understanding of succession at developing and self stabilising cell          industrial
                 B.V.                7742                                                    d            eiscosanoids, PAF,         populations and response/adaptation in the membrane lipid composition
                                                                                                          biomarker                  to stress conditions. Reveal cell signaling processes and interactions
                                                                                                                                     with the environment depending on lipid molecules. Lipid synthesis and
                                                                                                                                     degradation depend on enzyme activities and specific genes.
                                                                                                                                     Supressing specific lipid synthesis process or facilitating adaptation
                                                                                                                                     processes may identify genes involved and clarify function of so far
                                                                                                                                     unknown gene sequences. Assessment of regulatory lipids
                                                                                                                                     (eicosanoids, PAFs) as presymptomatic harbingers of pulmonary
                                                                                                                                     pathobiology. Development of rapid and easy-to-use methods for
                                                                                                                                     assessemnt and analysis of phospholipid profiles based on mass
                                                                                                                                     spectrometry. Rapid and sensitive quantification of signaling lipid
                                                                                                                                     molecules. Since 2007 LC-MS Support for Switzerland and South of
                                                                                                                                     germany Area, providing expertise in Lipidanalysis by LC-MS
Life Sciences    School of           +41-22-          1211    Geneva     Bd Yvoy 20          Switzerlan   Mass spectrometry,         please fill in                                                               scientific
Mass             Pharmaceutical      3796344                                                 d            Pharmaceuticals,
Spectrometry     Sciences -                                                                               Proteins, C.elegans
                 University of
                 Geneva
University of    University          +41 61           4031    Basel      Hebelstrasse, 20    Switzerlan   immune recognition of      Identification of llipids with immunogenic activity and involved in          scientific
Basel,           Hospital,           2652365                                                 d            lipids                     activating lipid-specific T cells in diseases
Experimental     Department of
Immunology       Research
Division of      University of       0041 26 300      CH-     Fribourg   5, chemin du        Switzerlan   Ceramide, GPI, cell        Biosynthesis, Remodeling and transport and cell wall integration of GPI      scientific
Biochemistry     Fribourg            8630             1700               musée               d            wall, heat shock,          anchored proteins of Saccharomaces cerevisiae. Sphingolipid
                                                                                                          cholesterol, ergosterol,   biosynthesis of S. cerevisiae. Sterol transport, neutral lipid storage and




                                                                                                                                                                                                           48
                                                                                                      steryl esters, lipases,    degradation. Lipid rafts transport and sortining of integral membrane
                                                                                                      lipid bodies               proteins.
Institute of       Academia Sinica      ++886-2-      11529   Taipei      Nankang           Taiwan    farnesyl                   Prenyl pyrophosphates as differentiation signals for stem cells               scientific
Cellular and                            27899531                                                      pyrophosphate,
Organismic                                                                                            geranylgeranyl
Biology, Section                                                                                      pyrophosphate, E-Ras
of Stem Cells
Institute of       National Tsing       886-3-        30043   Hinchu      Kung-Fu Rd        Taiwan    glycosphingolipid,         Our main interest is to understand the role of various lipids and/or lipid    scientific
Bioinformatics     Hua University       5742752                                                       cardiotoxin,               domain responsible for the action of cobra venom components such as
and Structural                                                                                        phospholipase A2           cobra cardiotoxins or phospholipase A2.
Biology
Dept. of Medical   Cardiff University   0044 29       CF14    Cardiff     Heath Park        United    please fill in             please fill in                                                                scientific
Biochemistry &                          2074 8447     4XN                                   Kingdom
Immunology,
School of
Medicine
Chemical           Chemical and         +44-          S3 7RD Sheffield    40 LeavyGreave    United    Sulfolipids,               My area of interest is to mining lipid profile of cyanobacteria for           scientific
Engineering Life   Process              0780971735                        Road              Kingdom   sulfoglycolipids,          identifying potential drug candidates.
Science            Engineering          5                                                             cyanobacteria,
Interface          University of                                                                      bioactive compounds
                   Sheffield
School of Life     Heriot-Watt          ++44 131      EH14    Edinburgh   Roccarton         United    Diet-gene interaction,     We are interested in the influence of diet on the regulation of gene          scientific
Sciences           University           451 3186      4AS                                   Kingdom   lipogenesis,fungal lipid   expression in hepatic tissue culture, in particular emphasis on lipogenic
                                                                                                      metabolism                 genes. A second area of interest focusses on fungal lipid metabolism,
                                                                                                                                 particularly in the oleaginous Yarrowia lipolytica.
The Chemical       Imperial College   +44 (0)20       SW7     London      Exhibition Road   United    Lipid biophysical          Biophysical properties of lipids Phase behaviour studied by SAXRD,            scientific
Biology Centre     London in          7594 5787       2AZ                                   Kingdom   characterisation           NMR, SSNMR, polarising microscopy, DSC, high pressure SAXRD
in the             association with                                                                                              Mesophase structure and energetics measured by SAXRD osmometry,
Department of      the Institute of                                                                                              SSNMR, ITC, DSC, direct           vesicle manipulation and modelling using
Chemistry          Cancer Research                                                                                               continuum elastic theories, mesoscopic modelling and full atomistic
                   and the London                                                                                                modelling (QMMD) Dynamics of phase transformations (the lipids role
                   Research Institute                                                                                            in cell division, fusion etc.) using     synchrotron X-ray scattering on
                   of CRUK                                                                                                       rapidly perturbed systems Studies of the short and long range
                                                                                                                                 coupling of membrane charge and curvature elasticity Lipid-protein and
                                                                                                                                 lipid-drug interactions Studies of the effect of membrane elasticity on
                                                                                                                                 enzyme activity, extrinsic membrane protein binding, membrane
                                                                                                                                 protein refolding dynamics, drug binding Studies of the coupling of
                                                                                                                                 membrane chemistry and biochemistry to lipid elastic properties
                                                                                                                                 Studies of lipid liquid ordered phases and protein associations within
                                                                                                                                 these domains Studies of membrane protein assembly, distribution
                                                                                                                                 and dynamics Development of novel single cell membrane proteomics
                                                                                                                                 and lipidomics A recently funded project to handle single cells inoptical
                                                                                                                                 traps within microfluidic flows,      strip off membrane portions, separate
                                                                                                                                 components and analyse using a novel 2D-IR spectroscopy
Nature Reviews     NPG                  +44(0)20784   N1      London      4 Crinan St       United    lipids                     lipids                                                                        scientific
Molecular Cell                          33641         9XW                                   Kingdom
Biology
Glycobiology       Oxford University    +44 1865      OX1     Oxford      South Parks Rd    United    Oligosaccharide            Protein and lipid glycosylation and diseases, including the lysosomal         scientific




                                                                                                                                                                                                       49
Institute                          275725       3QU                                   Kingdom   analysis, lysosomal        storage disorders (Gaucher disease for example). Glycolipid
                                                                                                storage                    (sphingolipid) analysis by HPLC and mass spectrometry. Chemistry and
                                                                                                                           biology of imino sugars that modulate glycolipid biosynthesis
Shell Global     Shell Global      +44-151-     CH13S   Chester     P.O. Box 1        United    extraction analysis        Interest in extraction and analysis techniques for lipids                   industrial
Solutions (UK)   Solutions (UK)    373-5730     H                                     Kingdom
Refsum disease   St Thomas         ++44207188   SE1     London      Lambeth Palace    United    peroxisome, alpha-         The group has been interested in the transport and biochemical              scientific
group            Hospital          1256         7EH                 Road              Kingdom   oxidation, omega-          pathways of phytanic acid metabolism and thus alpha- and omega-
                                                                                                oxidation, isoprenoid,     oxidation pathways of alpha-methyl isoprenoid fatty acids. This pathway
                                                                                                phytanic                   has recently been defined with the cloning of key enzymes in alpha-
                                                                                                                           oxidation including phytanoyl-CoA 2-hydroxylase (PAHX), 2-
                                                                                                                           hydroxyphytanoyl-CoA lyase, alpha-methylacyl-CoA racemase together
                                                                                                                           with confirmation of their localisation in peroxisomes. PAHX, an iron(II)
                                                                                                                           and 2-oxoglutarate dependent oxygenase is located on chromosome
                                                                                                                           10p13. Mutant forms of PAHX have been shown to be responsible for
                                                                                                                           some, but not all, cases of Refsums Disease. Certain cases have been
                                                                                                                           shown to be atypical mild variants of rhizomelic chondrodysplasia
                                                                                                                           punctata type 1a. Other atypical cases with low plasma phytanic acid
                                                                                                                           may be caused by a-methylacyl-CoA racemase deficiency. A sterol-
                                                                                                                           carrier protein 2 (SCP-2) knockout mouse model shares a similar clinical
                                                                                                                           phenotype to Refsums Disease, but no mutations in SCP-2 have been
                                                                                                                           described to date in man. SCP-2 acts as a solubilistaion
                                                                                                                           factor/intracellular carrier for these hydrophobic fatty acids. Work on
                                                                                                                           the omega-oxidation pathway for these fatty acids has clarified the role
                                                                                                                           of cytochrome 4A1 enzymes in the intitial hydroxylation to dicarboxylic
                                                                                                                           acids and the subsequent peroxisomal beta-oxidation pathway. It has
                                                                                                                           also led to interest in this pathway as a therapeutic option for
                                                                                                                           peroxiosomal diseases. The pathway for synthesis of phytanic acid
                                                                                                                           from phytol in man and possibly other mammals has also been clarified
                                                                                                                           with the identification of the enzymes in the pathway and identification of
                                                                                                                           FALDH-10 - the enzyme deficient in Sjogren-Larsson syndroem as one
                                                                                                                           of the key enzymes in this pathway. The group is also interested in the
                                                                                                                           role of PhyH outside the preroxisome where it may act as protein
                                                                                                                           regulator and the possible importance of phytanic acid as a PPAR-alpha
                                                                                                                           ligand.
Mass             The School of     ++44-20-     WC1N    London      29/39 Brunswick   United    sterols, steroids, lipid   The research in the mass spectrometry group at the School of                scientific
Spectrometry     Pharmacy          77535876     1AX                 Square            Kingdom   rafts                      Pharmacy is focused on lipids and their interactions with proteins. Major
Facility                                                                                                                   efforts are being made in the development of new methodology for the
                                                                                                                           mass spectrometric analysis of steroids and sterols. In parrallel with
                                                                                                                           lipidomic studies, we are investigating the protein content of lipid rafts
                                                                                                                           and studying protein-lipid interactions.
European         Thermo Electron   +44          HP2     Hemel       1 Boundary Park   United    mass spectrometry,         methods in mass spectrometry for high throughput lipid analysis             industrial
application                        1442233555   7GE     Hempstead                     Kingdom   phospholipids,
laboratory                                                                                      sphingolipids
WELLCOME         UNIV OF           ++44-        DD1     DUNDEE      HAWKHILL          United    PROTOZA LIPID              MY GROUP WORKS ON PHOSPHO- AND GLYCO- LIPID                                 scientific
TRUST            DUNDEE            (0)1382-     5EH                                   Kingdom   BIOSYNTHESIS               BIOSYNTHESIS IN PROTOZOAN PARASITES SUCH AS T.BRUCEI,
BIOCENTRE,                         388688                                                                                  THE CAUSATIVE AGENT OF AFRICAN SLEEPING SICKNESS. WE
SCHOOL OF                                                                                                                  USE TOOLS SUCH AS FOWARD AND REVESRE GENETICS TO
LIFE                                                                                                                       VALIDATE GENES AS DRUG TARGETS PRIOR TO EXPLORING




                                                                                                                                                                                                50
SCIENCES,                                                                                                                       SUBSTRATE AND INHIBITOR SELECTIVITY. WE HAVE START TO
UNIV OF                                                                                                                         SET UP A PARASITE LIPIIDOME DATABASE FOR THE COMMUNITY
DUNDEE                                                                                                                          TO USE AND SUBMIT PROFILES TO.
Cardiovascular    University College +44 207 679     WC1E   London       5 University St      United    genes, apolipoproteins, Cardiovascular Genetics                                                         scientific
Genetics,         London             6968            6JF                                      Kingdom   triglyceridie
                                                                                                        metabolism
Aberdeen          University of        44 (0)1224    AB25   Aberdeen     Polwarth building,   United    inflammation-           function of biologically active lipids in cardiovascular disorders. Effect of   scientific
lipidomix group   Aberdeen             553020        2ZD                 Foresterhill         Kingdom   atherosclerosis-        dietary components on the expression and secretion of biologically
                                                                                                        endothelial fonction-   active lipid derivatives in relation to inflammatory disorders.
                                                                                                        diet
Department of     University of Bath   ++-44-1225-   BA2    Bath         Claverton Down       United    Enzyme, phytanic acid, Enzaymes involved in lipid metabolism, branched-chain lipids and their           scientific
Pharmacy &                             3867686       7AY                                      Kingdom   isoprenoids, long-chain role in diseases, long-chain fatty alcohols,
Pharmacology                                                                                            fatty alcohols
Institute for     University of        44-(0)121-    B15    Birmingham   Vincent Drive        United    phosphoinositide,       1. Analysis of lipid signalling in particular phospholipases D and C and        scientific
Cancer Studies    Birmingham           414-3293      2TT                                      Kingdom   mass spectrometry,      PI-3-kinase. 2. Use of MS methods to analyse lipids in mammalian,
                                                                                                        phospholipase, cancer dictyostelium, yeast and drosophila cells in order to determine functions
                                                                                                                                of signalling pathways. 3. Analysis of lipids in tumour cells isolated by
                                                                                                                                techniques such as laser capture microdissection. 4. Analysis of
                                                                                                                                changes in lipids in other diseases for example vasculitis. 5.
                                                                                                                                Development of MS methods to quantify all phosphoinositides.
School of         University of        ++44-1274-    BD7    Bradford     Richmond Road        United    eicosanoids, brain,     eicosanoids and other lipid mediators molecular mechanism of action of          scientific
Pharmacy          Bradford             224717        1DP                                      Kingdom   skin, mass              omega-3 fatty acids with emphasis on eicosapentaenoic acid
                                                                                                        spectrometry            eicosanoid-mediated pathways and signalling systems mass
                                                                                                                                spectrometry high field NMR computational lipidomics cardiovascular
                                                                                                                                disease neurodegenerative diseases brain function cancer melanocytes
Department of     University of        44-1223-      CB2    Cambridge    Tennis Court         United    multidrug transporters, We study the molecular bases of the interactions of ATP-binding                 scientific
Pharmacology      Cambridge            334032        1PD                 Road                 Kingdom   steroids,               cassette transporters of human (e.g. ABCG1 and ABCG2) and bacterial
                                                                                                        structure/function      origin (e.g., MsbA and LmrA) with chemotherapeutic drug and steroids
                                                                                                                                and other lipids. We also study the potential physiological roles of
                                                                                                                                multidrug transporters in lipid transport.
Dept. of          University of        +44 1865      OX1    Oxford       South Parks Road United        membrane protein        membrane protein/lipid interactions biomolecular simulations                    scientific
Biochemistry      Oxford               275371        3QU                                  Kingdom
School of         University of        ++44          SO17   Southampto   University Road  United        phospholipids, mass       Mass spectrometry of lipids Dynamics of phospholipid synthesis              scientific
Chemistry         Southampton          (0)2380       1BJ    n                             Kingdom       spectrometry, dynamic     Modelling lipid synthetic networks Lipid biomarkers in health and
                                       796161                                                           lipidomics                disease Synthesis, composition and function of endonuclear lipids Lung
                                                                                                                                  surfactant in health and disease Interaction between genotypic
                                                                                                                                  expression and diet in the regulation of the molecular species
                                                                                                                                  composition of cell lipids in vivo and in vitro Phospholipase-mediated cell
                                                                                                                                  signalling Membrane fusion Lipids in stem cell differentiation Lipids and
                                                                                                                                  nutrition Lipids in inflammation Oxidised phospholipids and
                                                                                                                                  cardiovascular disease
Centre for        Writtle College      44 1245       CM1    Chelmsford   Lordship Road        United    poyunsaturated fatty      Nutritional and biochemical role of fatty acids in the health and           scientific
Equine and                             424200        3RR                                      Kingdom   acids, conjugated         performance of farm and companion animals.
Animal Science                                                                                          linoeic acids, immunity,
                                                                                                        health
Centre for        Writtle College      44 1245       CM1    Chelmsford   Lordship Road        United    poyunsaturated fatty     Nutritional and biochemical role of fatty acids in the health and              scientific
Equine and                             424200        3RR                                      Kingdom   acids, conjugated        performance of farm and companion animals.
Animal Science                                                                                          linoeic acids, immunity,




                                                                                                                                                                                                        51
                                                                                                         health
University of    Dept. of Molecular 1-319-335-        52246   Iowa City      6-530 Bowen        United   multidrug resistance,         We study the interaction of sphingolipids and phospholipids with               scientific
Iowa             Physiology and     7874                                     Science Building   States   sphingolipids,                multidrug transporters in the yeast Saccharomyces cerevisiae.
                 Biophysics                                                                              mitochondria                  Transcription factors that modulate the expression of membrane
                                                                                                                                       transporters, often in the plasma membrane, have recently been found
                                                                                                                                       to also control the expression of genes involved in sphingolipid
                                                                                                                                       biosynthesis. Our goal is to understand the physiological rationale this
                                                                                                                                       coordinate control.
Indiana          Indiana             317-274-         46202   Indianapolis   723 W. Michigan    United   yeast, sterol,                yeast sterol biosynthesis                                                      scientific
Umiversity -     Umiversity -        0593                                    St.                States   ergosterol
Purdue           Purdue University
University       Indianapolis
Indianapolis
Institute of     Medical College     01-706-721-      GA      Augusta        1120 15th Street   United   Sphiogolipids,                Analysis of glycolipid structure and metabolism in the nervous system,         scientific
Molecular        of Georgia          0699             30912                                     States   glycolipids,                  lipid signaling molecules and signal trnaduction, cell-cell recognition and
Medicine and                                                                                             developmental biology,        adhesion, cell migration.
Genetics                                                                                                 neurodegenerative
                                                                                                         diseases,
                                                                                                         neurochemistry
COBRE in         Medical University 843-792-          29425   Charleston     PO Box 250509      United   cell growth, cell death,      Define the function of these fatty molecules in human disease,                 scientific
Lipidomics &     of South Carolina 4323                                                         States   cell aging,                   especially cancer, aging, neurologic disease and fungal pathogenesis.
Pathobiology                                                                                             inflammation
COBRE in         Medical University 843-792-          29425   Charleston     PO Box 250509      United   cell growth, cell death,      Define the function of these fatty molecules in human disease,                 scientific
Lipidomics &     of South Carolina 4323                                                         States   cell aging,                   especially cancer, aging, neurologic disease and fungal pathogenesis.
Pathobiology                                                                                             inflammation
Center for       Stony Brook         please fill in   11794   Stony Brook    438 CMM            United   please fill in                please fill in                                                                 scientific
Developmental    University                                                                     States
Genetics
The Polt Group   The University of   ++01-520-        AZ      Tucson         Department of      United   PDMP,                         Glycosphingolipids. Synthesis and structure elucidation. We are                scientific
                 Arizona             621-6322         85721                  Chemistry          States   glycosphingolipid,            involved with the design and synthesis of glycosidase and
                                                                                                         sphingosine                   glycosyltransferase inhibitors. We use Manduca sexta (tobacco horn
                                                                                                                                       worm) as a model system to explore the effects of GSLs on
                                                                                                                                       development.
                                                                                                                                       http://www.chem.arizona.edu/faculty/profile/profile.php?fid_call=polt
San Diego        University of       858-822-         92093   La Jolla       9500 Gilman        United   lipid classification, lipid   The LIPID Metabolites and Pathways Strategy (LIPID MAPS)                       scientific
Supercomputer    California San      3619                                    Drive, Mail Code   States   database, lipid               Consortium represents a multi-institutional effort to develop a detailed
Center / LIPID   Diego                                                       0412                        proteome, lipid               understanding of lipid structure and function. As part of this effort, we will
MAPS                                                                                                     pathways                      develop ‘parts lists’ of lipid metabolites and assemble these into
Bioinformatics                                                                                                                         metabolic networks. These networks will then provide an infrastructure
Core                                                                                                                                   for subsequent modeling using quantitative data from LIPID MAPS
                                                                                                                                       experiments.
Nutrition and    USDA-Human          ++1-617-         02111   Boston         711 Washington     United   genetic                       The Nutrition and Genomics Laboratory has been pionnering the study            scientific
Genomics         Nutrition           556-3102                                St                 States   polymorphisms, gene-          of gene-diet interactions in the area of cardiovascular diseases, utilizing
                 Research Center                                                                         diet interactions,            both genetic epidemiology approaches as well as controlled dietary
                 on Aging at Tufts                                                                       perilipins,                   intervention studies. This research involves the investigation of nutrient-
                 University                                                                                                            gene interactions in large and diverse populations around the world with
                                                                                                                                       long-standing collaborations with investigators in Europe, Asia, Australia
                                                                                                                                       and the United States. More recently our interest has been focusing on




                                                                                                                                                                                                               52
                                                                                                                            the genetic factors involved in fat metabolism in the adypocite and more
                                                                                                                            specifically the PATS family of genes. In addition, we seek to identify
                                                                                                                            genes involved in longevity and healthy aging and to understand their
                                                                                                                            regulation in response to dietary factors. For this research we use model
                                                                                                                            organisms such as Drosophila and mouse. Further comprehension of
                                                                                                                            the relation between genetic factors, nutrients and the rate of aging will
                                                                                                                            provide better understanding of the pathology of age-related diseases
                                                                                                                            and lead to improved strategies for their prevention.
Department of   Wake Forest         please fill in   27157   Winston-   Medicial Center   United   phospholipase D,         The Department of Biochemistry (and affiliated faculty) at Wake Forest       scientific
Biochemistry    University School                            Salem      Boulevard         States   bicelles, eicosanoids,   University has a working group of faculty members (about 14 faculty
                of Medicine                                                                        lipoproteins, lipid      members) interested in the role of lipids in signal transduction,
                                                                                                   kinases, virus           atherosclerosis, cancer, host defense, and inflammation. Techniques in
                                                                                                   assesmbly                use include thin-layer chromatography, HPLC, and mass spectrometry
                                                                                                                            to identify phospholipid and fatty acid species, vesicle and solid-phase
                                                                                                                            lipid binding assays, NMR spectroscopy and other biophysical
                                                                                                                            approaches, coupled with site-directed mutagenesis, to study structural
                                                                                                                            features of lipid:protein interactions, and computational modeling of
                                                                                                                            signaling pathways involving lipids. Professor McPhail represents the
                                                                                                                            lipid signaling working group on the departmental Development
                                                                                                                            Committee, which advises the Chair on departmental development and
                                                                                                                            policy.




                                                                                                                                                                                                 53
Appendix 2: Lipidomics expertise survey results ordered by country and Institute (Expertise level ≥ 2; 0 = weak, 3 = strong;
LEP as per 01.01.2008)
Austria

                                                                                                                                                                Organisms and cellular Systems -
Institute                                 Areas of Lipidomics Expertise          Lipid Class - Survey                     Technology - Survey
                                                                                                                                                                Survey
IMB Biochemistry - Yeast Genetics         Analytical Tools in Lipidomics,         Fatty Acids and Conjugates [FA01],      Confocal Microscopy, Enzymatic        Yeast,
Group                                     Genomics of the Lipidome, Lipid                                                 Assays, Fluorescence Microscopy, GC,
University of Graz                        Metabolism, Lipids in Microbiology,                                             Gel Electrophoresis, Light Microscopy,
(Austria)                                 Proteomics of the Lipidome,                                                     Thin layer chromatography,
Institute for Genomics and                Bioinformatics related to the Lipidome,                                         Bioinformatics, Biostatistics, Cluster Human Cell Lines, Mice, Mouse,
Bioinformatics                            Genomics of the Lipidome, Lipid                                                 Analysis, Data Processing, Database
Graz University of Technology             Metabolism,                                                                     Setup, Fluorescence Microscopy, Light
(Austria)                                                                                                                 Microscopy, Microarrays, Real Time
                                                                                                                          PCR,
Institute for Molecular Biotechnology     Genomics of the Lipidome, Lipid        Sterols [ST01],                          DNA Sequencing, Enzymatic Assays, Eubacteria, Yeast,
Graz University of Technology             Metabolism, Lipid Transport,                                                    Fluorescence Microscopy, GC, GC-MS
(Austria)                                 Membrane Microdomains, Membrane                                                 coupling, Gel Electrophoresis, Immuno
                                          Trafficking,                                                                    Assays, Light Microscopy, Real Time
                                                                                                                          PCR, Thin layer chromatography, UV-
                                                                                                                          Vis Spectroscopy,
Institute of Biochemistry, Cell Biology   Analytical Tools in Lipidomics,         Glycerophosphocholines [GP01],          Enzymatic Assays, Fluorescence         Yeast,
Group                                     Genomics of the Lipidome, Lipid         Glycerophosphoethanolamines [GP02],     Microscopy, GC, Gel Electrophoresis,
Graz University of Technology             Metabolism, Lipid Transport, Lipids in Glycerophosphoinositols [GP06],          Immuno Assays, MALDI, Thin layer
(Austria)                                 Microbiology, Membrane                  Glycerophosphoserines [GP03], Sterols   chromatography, Two dimensional
                                          Microdomains, Membrane Trafficking, [ST01],                                     Electrophoresis,
                                          Metabolomics of the Lipidome,
                                          Proteomics of the Lipidome,
Molecular Biology and Biochemistry,       Genomics of the Lipidome, Lipid
Medical University Graz                   Metabolism, Lipid Transport, Lipids and
(Austria)                                 Nutrition, Metabolomics of the
                                          Lipidome, Pharmacology and
                                          toxicology of the Lipidome, Signalomics
                                          of the Lipidome,

Belgium

                                                                                                                                                                  Organisms and cellular Systems -
Institute                                 Areas of Lipidomics Expertise           Lipid Class - Survey                    Technology - Survey
                                                                                                                                                                  Survey
Div. Pharmacology - Dep. Mol. Cell                                                Ceramides [SP02], Diradylglycerols      Chemical Synthesis, Enzymatic Assays, Eubacteria, Human Cell Lines, Human
Biology                                                                           [GL02], Fatty Acids and Conjugates      GC, Gel Electrophoresis, Ion Exchange, Primary, Mice, Mouse, Rats,
K.U.Leuven                                                                        [FA01], Fatty aldehydes [FA06],         LC, Liquid-Liquid Extraction, Normal
(Belgium)                                                                         Fattyamides [FA08], Isoprenoids         Phase, Reversed Phase, Size
                                                                                  [PR01], Neutral glycosphingolipids      Exclusion, Solid Phase Extraction, Thin
                                                                                  [SP05], Polyprenols [PR03], Sphingoid layer chromatography, UV-Vis
                                                                                  bases [SP01], Triradylglycerols [GL03], Spectroscopy,



                                                                                                                                                                                                   54
Laboratory for Experimental Medicine    Analytical Tools in Lipidomics,                                                 DNA Sequencing, Enzymatic Assays, Biopsy, Human Cell Lines, Mice,
and Endocrinology (LEGENDO)             Genomics of the Lipidome, Lipid                                                 ESI, Fluorescence Microscopy, Gel
Katholieke Universiteit Leuven          Metabolism, Mapping of the Lipidome,                                            Electrophoresis, Immuno Assays, Light
(Belgium)                               Membrane Microdomains,                                                          Microscopy, Microarrays, nano ESI,
                                        Metabolomics of the Lipidome,                                                   Real Time PCR, Thin layer
                                        Signalomics of the Lipidome,                                                    chromatography,

Denmark

                                                                                                                                                               Organisms and cellular Systems -
Institute                               Areas of Lipidomics Expertise             Lipid Class - Survey                  Technology - Survey
                                                                                                                                                               Survey
Biochemistry and Nutrition Group,       Analytical Tools in Lipidomics,         Ceramides [SP02], Diradylglycerols      APCI, Chemical Synthesis, CI, Cluster Biopsy, Blood, Mice, Rats, Stool,
Biocentrum-DTU                          Chemical Synthesis of Lipids            [GL02], Docosanoids [FA04],             Analysis, EI, Enzymatic Assays, ESI,
The Technical University of Denmark     (Probes/Tools/Agents), Lipid            Eicosanoids [FA03], Fatty Acids and     GC, GC-MS coupling, Immune Affinity,
(Denmark)                               Metabolism, Lipid Signalling, Lipid     Conjugates [FA01], Fatty esters [FA07], Ion Exchange, LC, LC-MS coupling,
                                        Standard Bank, Lipid Transport, Lipids Glycerophosphocholines [GP01],           Liquid-Liquid Extraction, Near Field,
                                        and Nutrition, Mapping of the Lipidome, Glycerophosphoethanolamines [GP02], Normal Phase, Quadrupole, Reversed
                                        Metabolomics of the Lipidome,           Glycerophosphoglycerophosphoglycerols Phase, Size Exclusion, Solid Phase
                                        Nutrigenomics of the Lipidome,          (Cardiolipins) [GP12],                  Extraction, Thin layer chromatography,
                                                                                Glycerophosphoinositols [GP06],         Two dimensional Electrophoresis, UV-
                                                                                Glycerophosphoserines [GP03],           Vis Spectroscopy,
                                                                                Monoradylglycerols [GL01],
                                                                                Octadecanoids [FA02],
                                                                                Phosphosphingolipids [SP03], Sphingoid
                                                                                bases [SP01], Sterols [ST01],
                                                                                Triradylglycerols [GL03],

Finland

                                                                                                                                                              Organisms and cellular Systems -
Institute                               Areas of Lipidomics Expertise             Lipid Class - Survey                  Technology - Survey
                                                                                                                                                              Survey
Institute of Biomedicine/Käkelä Group   Mapping of the Lipidome, Signalomics
University of Helsinki                  of the Lipidome,
(Finland)
Institute of Biomedicine/Somerharju     Analytical Tools in Lipidomics,           Acidic glycosphingolipids [SP06],     Chemical Synthesis, Cluster Analysis, Human Cell Lines,
group                                   Bioinformatics related to the Lipidome,   Ceramides [SP02], Diradylglycerols    Confocal Microscopy, Data Processing,
University of Helsinki                  Chemical Synthesis of Lipids              [GL02], Fatty Acids and Conjugates    Database Setup, Enzymatic Assays,
(Finland)                               (Probes/Tools/Agents), Lipid              [FA01], Glycerophosphates [GP10],     ESI, Fluorescence Microscopy,
                                        Metabolism, Lipid Standard Bank, Lipid    Glycerophosphocholines [GP01],        Fluorescence Phosphorescence
                                        Transport, Mapping of the Lipidome,       Glycerophosphoethanolamines [GP02], Spectroscopy, Ion Trap, LC, LC-MS
                                        Membrane Microdomains, Membrane           Glycerophosphoglycerols [GP04],       coupling, Light Microscopy, Liquid-
                                        Trafficking, Metabolomics of the          Glycerophosphoglycerophosphates       Liquid Extraction, Normal Phase,
                                        Lipidome, Proteomics of the Lipidome,     [GP05],                               Quadrupole, Reversed Phase, Solid
                                                                                  Glycerophosphoglycerophosphoglycerols Phase Extraction, Thin layer
                                                                                  (Cardiolipins) [GP12],                chromatography, Triple Quadrupole,
                                                                                  Glycerophosphoinositol bisphosphates
                                                                                  [GP08], Glycerophosphoinositol




                                                                                                                                                                                                  55
                                                                                monophosphates [GP07],
                                                                                Glycerophosphoinositols [GP06],
                                                                                Glycerophosphoserines [GP03],
                                                                                Monoradylglycerols [GL01], Neutral
                                                                                glycosphingolipids [SP05],
                                                                                Phosphosphingolipids [SP03], Sterols
                                                                                [ST01], Triradylglycerols [GL03],
Quantitative Biology and Bioinformatics Analytical Tools in Lipidomics,         Ceramides [SP02], Diradylglycerols     APCI, Biostatistics, Cluster Analysis, Biopsy, Blood, Human Cell Lines,
VTT Technical Research Centre of        Bioinformatics related to the Lipidome, [GL02], Docosanoids [FA04],            Data Processing, Database Setup, ESI, medicinal plants, Mice, Rats, Urine,
Finland                                 Glycomics of the Lipidome, Lipid        Eicosanoids [FA03], Fatty Acids and    GC, GC-MS coupling, Ion Exchange, Yeast,
(Finland)                               Metabolism, Lipid Standard Bank,        Conjugates [FA01],                     Ion Trap, LC, LC-MS coupling,
                                        Lipids and Nutrition, Lipids in         Glycerophosphocholines [GP01],         Microarrays, nano ESI, Normal Phase,
                                        Inflammation, Mapping of the Lipidome, Glycerophosphoethanolamines [GP02], Nuclear Magnetic Resonance, QqTOF,
                                        Metabolomics of the Lipidome,           Glycerophosphoglycerophosphoglycerols Quadrupole, Real Time PCR, Reversed
                                        Nutrigenomics of the Lipidome,          (Cardiolipins) [GP12],                 Phase, Size Exclusion, Thin layer
                                        Pharmacology and toxicology of the      Glycerophosphonocholines [GP16],       chromatography, TOF, Triple
                                        Lipidome,                               Glycerophosphonoethanolamines          Quadrupole,
                                                                                [GP17], Glycerophosphoserines [GP03],
                                                                                Isoprenoids [PR01], Monoradylglycerols
                                                                                [GL01], Neutral glycosphingolipids
                                                                                [SP05], Octadecanoids [FA02], Oxidized
                                                                                glycerophospholipids [GP20],
                                                                                Phosphosphingolipids [SP03], Sphingoid
                                                                                bases [SP01], Sterols [ST01],
                                                                                Triradylglycerols [GL03],

France

                                                                                                                                                           Organisms and cellular Systems -
Institute                              Areas of Lipidomics Expertise          Lipid Class - Survey                     Technology - Survey
                                                                                                                                                           Survey
Biological Chemistry Laboratory    Analytical Tools in Lipidomics, Lipid    Fatty Acids and Conjugates [FA01], Fatty Enzymatic Assays, ESI, GC, GC-MS      A thaliana, Agricultural Crop,
Biological Chemistry               Metabolism, Lipids in Microbiology,      ethers [FA10], Steroids [ST02], Sterols coupling, Gel Electrophoresis, Immuno Arabidopsis, Eubacteria, S cerevisae, Y
LaboratoryNational Institute for   Proteomics of the Lipidome,              [ST01], Triradylglycerols [GL03],        Assays, LC, LC-MS coupling, Reversed lipolytica, Yeast,
Agronomic Research (INRA)                                                                                            Phase, Thin layer chromatography,
(France)                                                                                                             Two dimensional Electrophoresis, UV-
                                                                                                                     Vis Spectroscopy,
IMBL-Lipid Signaling (UMR 585      Analytical Tools in Lipidomics, Lipid    Docosanoids [FA04], Eicosanoids          Brewster Angle Microscopy (BAM), Cell Blood, Human Cell Lines, Human
INSERM / INSA-Lyon)                Metabolism, Lipid Signalling, Lipids and [FA03], Fatty Acids and Conjugates       Sorting, Confocal Microscopy, EI,     Primary, Urine,
INSA-Lyon and CNRS / INSERM / Univ Nutrition, Membrane Microdomains,        [FA01], Fatty aldehydes [FA06],          Enzymatic Assays, Fluorescence
Lyon1                              Metabolomics of the Lipidome,            Glycerophosphocholines [GP01],           Microscopy, GC, GC-MS coupling,
(France)                           Nutrigenomics of the Lipidome,           Glycerophosphoethanolamines [GP02], Immuno Assays, Ion Trap, LC, Liquid-
                                   Signalomics of the Lipidome,             Oxidized glycerophospholipids [GP20], Liquid Extraction, NCI, Normal Phase,
                                                                                                                     Quadrupole, Reversed Phase, Solid
                                                                                                                     Phase Extraction, Thin layer
                                                                                                                     chromatography,
INSERM U467/IFR94 Proteomics       Analytical Tools in Lipidomics, Lipid    Diradylglycerols [GL02], Eicosanoids     Cell Sorting, Confocal Microscopy,    Biopsy, Blood, Human Cell Lines, Mice,
Platform                           Metabolism, Lipid Signalling, Lipids and [FA03], Fatty Acids and Conjugates       DNA Sequencing, Enzymatic Assays, Mouse, Urine,
Faculté de Médecine René Descartes Nutrition, Lipids in Inflammation,       [FA01], Glycerophosphocholines [GP01], ESI, Fluorescence Microscopy, GC,




                                                                                                                                                                                                    56
Paris 5                                Mapping of the Lipidome, Membrane      Glycerophosphoethanolamines [GP02], GC-MS coupling, Gel Electrophoresis,
(France)                               Microdomains, Membrane Trafficking,    Glycerophosphoglycerols [GP04],          Immuno Assays, Ion Trap, LC, LC-MS
                                       Proteomics of the Lipidome,            Glycerophosphoglycerophosphates          coupling, Light Microscopy, Liquid-
                                                                              [GP05], Glycerophosphoinositols [GP06], Liquid Extraction, MALDI, Multicolor
                                                                              Glycerophosphoserines [GP03],            Analysis, nano ESI, Normal Phase,
                                                                              Monoradylglycerols [GL01], Sterols       Real Time PCR, Reversed Phase,
                                                                              [ST01], Triradylglycerols [GL03],        Solid Phase Extraction, Thin layer
                                                                                                                       chromatography, TOF, Two
                                                                                                                       dimensional Electrophoresis,
INSERM U586 (Obesity Research Unit) Analytical Tools in Lipidomics, Lipid     Fatty Acids and Conjugates [FA01],       Electron Microscopy, Enzymatic        Biopsy, Blood, Human Cell Lines,
INSERM,                              Metabolism, Lipid Signalling, Lipids and Other [GL00], Other [GP00],              Assays, Fluorescence Microscopy,      Human Primary, Mice, Mouse, Rats,
(France)                             Nutrition, Lipids in Inflammation,       Triradylglycerols [GL03],                Liquid-Liquid Extraction, Real Time
                                     Metabolomics of the Lipidome,                                                     PCR, Thin layer chromatography, UV-
                                     Signalomics of the Lipidome,                                                      Vis Spectroscopy,
INSERM U671                          Analytical Tools in Lipidomics,          Fatty Acids and Conjugates [FA01], Fatty Cell Sorting, Confocal Microscopy,    Biopsy, Human Cell Lines, Human
INSERM                               Genomics of the Lipidome, Lipid          esters [FA07], Sterols [ST01],           DNA Sequencing, Enzymatic Assays, Primary, humans, Mice, Mouse, Rats,
(France)                             Metabolism, Lipid Transport, Lipids and Triradylglycerols [GL03],                 Fluorescence Microscopy, Gel
                                     Nutrition, Membrane Trafficking,                                                  Electrophoresis, Immuno Assays, Light
                                     Metabolomics of the Lipidome,                                                     Microscopy, Real Time PCR, Thin layer
                                     Nutrigenomics of the Lipidome,                                                    chromatography,
                                     Proteomics of the Lipidome,
                                     Signalomics of the Lipidome,
Institute of molecular and cellular  Analytical Tools in Lipidomics, Lipid                                             Confocal Microscopy, DNA              Human Cell Lines, Mice, Mouse,
pharmacology                         Signalling, Lipids in Inflammation,                                               Sequencing, Fluorescence Microscopy,
CNRS UMR6097                                                                                                           Light Microscopy, MALDI, Microarrays,
(France)                                                                                                               Real Time PCR,
Laboratoire de Biogenèse Membranaire Analytical Tools in Lipidomics,          Diradylglycerols [GL02], Fatty Acids and Confocal Microscopy, Enzymatic        Agricultural Crop, Arabidopsis, Mice,
CNRS UMR 5200 Université V.          Genomics of the Lipidome, Lipid          Conjugates [FA01], Glycerophosphates Assays, GC, GC-MS coupling, Gel           Yeast,
Segalen Bordeaux 2                   Metabolism, Lipid Signalling, Mapping [GP10], Glycerophosphocholines [GP01], Electrophoresis, Immune Affinity, Ion
(France)                             of the Lipidome, Membrane                Glycerophosphoethanolamines [GP02], Exchange, LC, Microarrays, Normal
                                     Microdomains, Membrane Trafficking, Glycerophosphoglycerols [GP04],               Phase, Real Time PCR, Size
                                     Metabolomics of the Lipidome,            Glycerophosphoglycerophosphates          Exclusion, Thin layer chromatography,
                                     Proteomics of the Lipidome,              [GP05],
                                                                              Glycerophosphoglycerophosphoglycerols
                                                                              (Cardiolipins) [GP12],
                                                                              Glycerophosphoinositol bisphosphates
                                                                              [GP08], Glycerophosphoinositol
                                                                              monophosphates [GP07],
                                                                              Glycerophosphoinositol trisphosphates
                                                                              [GP09], Glycerophosphoinositols [GP06],
                                                                              Glycerophosphonocholines [GP16],
                                                                              Glycerophosphonoethanolamines
                                                                              [GP17], Glycerophosphoserines [GP03],
                                                                              Glyceropyrophosphates [GP11],
                                                                              Hydrocarbons [FA11],
                                                                              Monoradylglycerols [GL01],
                                                                              Triradylglycerols [GL03],




                                                                                                                                                                                                57
Germany

                                                                                                                                                                      Organisms and cellular Systems -
Institute                                 Areas of Lipidomics Expertise              Lipid Class - Survey                   Technology - Survey
                                                                                                                                                                      Survey
Albrecht-von-Haller-Institute for Plant   Analytical Tools in Lipidomics,         Diradylglycerols [GL02], Eicosanoids      APCI, CI, EI, Enzymatic Assays, ESI,      Agricultural Crop, Algae, Arabidopsis,
Sciences/Plant Biochemistry               Genomics of the Lipidome, Lipid         [FA03], Fatty Acids and Conjugates        GC, GC-MS coupling, Gel                   Yeast,
Georg-August-University                   Metabolism, Lipid Signalling, Lipid     [FA01], Fatty aldehydes [FA06], Fatty     Electrophoresis, Ion Exchange, Ion
(Germany)                                 Standard Bank, Lipid Transport, Lipids  esters [FA07], Glycerophosphoinositol     Trap, LC, LC-MS coupling, Liquid-Liquid
                                          in Inflammation, Lipids in Microbiology,bisphosphates [GP08],                     Extraction, NCI, Normal Phase,
                                          Signalomics of the Lipidome,            Glycerophosphoinositol                    Quadrupole, Reversed Phase, Size
                                                                                  monophosphates [GP07],                    Exclusion, Solid Phase Extraction, Thin
                                                                                  Glycerophosphoinositol trisphosphates     layer chromatography, TOF, Two
                                                                                  [GP09], Glycerophosphoinositols           dimensional Electrophoresis,
                                                                                  [GP06], Monoradylglycerols [GL01],
                                                                                  Octadecanoids [FA02], Oxidized
                                                                                  glycerophospholipids [GP20],
                                                                                  Triradylglycerols [GL03],
department of cellular and molecular      Analytical Tools in Lipidomics,                                             Biostatistics, Cell Sorting, Cluster
                                                                                  Acidic glycosphingolipids [SP06], Basic                                      Biopsy, Mice, Mouse, Rats,
pathology                                 Bioinformatics related to the Lipidome, glycosphingolipids [SP07], CeramidesAnalysis, Confocal Microscopy, Data
DKFZ(German Cancer Research               Genomics of the Lipidome, Lipids in     [SP02], Fatty Acids and Conjugates  Processing, Electron Microscopy,
Center)                                   Inflammation, Membrane                  [FA01], Neutral glycosphingolipids  Fluorescence Microscopy, Gel
(Germany)                                 Microdomains, Membrane Trafficking, [SP05],                                 Electrophoresis, Immuno Assays, Light
                                                                                                                      Microscopy, Microarrays, nano ESI,
                                                                                                                      Real Time PCR, Thin layer
                                                                                                                      chromatography, Two dimensional
                                                                                                                      Electrophoresis,
Department of Physiological Chemistry Analytical Tools in Lipidomics,           Fatty Acids and Conjugates [FA01],    Biostatistics, Confocal Microscopy, Data Biopsy, Blood, Human Cell Lines,
University of Veterinary Medicine      Glycomics of the Lipidome, Membrane Fatty esters [FA07],                       Processing, DNA Sequencing,              Human Primary,
Hannover                               Microdomains, Membrane Trafficking, Glycerophosphocholines [GP01],             Enzymatic Assays, ESI, Fluorescence
(Germany)                              Proteomics of the Lipidome,              Glycerophosphoethanolamines [GP02], Microscopy, GC, Gel Electrophoresis,
                                                                                Glycerophosphoglycerols [GP04],       Immune Affinity, Ion Exchange, LC-MS
                                                                                Glycerophosphoinositols [GP06],       coupling, Light Microscopy, Normal
                                                                                Glycerophosphoserines [GP03], Neutral Phase, Real Time PCR, Reversed
                                                                                glycosphingolipids [SP05], Other      Phase, Thin layer chromatography, Two
                                                                                [FA00], Phosphosphingolipids [SP03], dimensional Electrophoresis,
                                                                                Steroids [ST02], Sterols [ST01],
Dept. of Neonatology, Lipid Metabolism Analytical Tools in Lipidomics, Lipid    Fatty Acids and Conjugates [FA01],    Biostatistics, Cell Sorting, Data        bird lungs, Blood, Human Primary,
and Developmental Physiology Group Metabolism, Lipid Transport,                 Glycerophosphocholines [GP01], Other Processing, dHPLC, ESI, Fluorescence induced sputa, lung lavage fluid, Rats,
Eberhard Karls Universität Tübingen    Metabolomics of the Lipidome,            [FA00], Phosphosphingolipids [SP03], Phosphorescence Spectroscopy, LC,
(Germany)                                                                                                             LC-MS coupling, Light Microscopy,
                                                                                                                      Liquid-Liquid Extraction, Normal Phase,
                                                                                                                      Quadrupole, Reversed Phase, Size
                                                                                                                      Exclusion, Solid Phase Extraction, Thin
                                                                                                                      layer chromatography, Triple
                                                                                                                      Quadrupole, UV-Vis Spectroscopy,
Institut for Food Chemistry            Analytical Tools in Lipidomics, Chemical Ceramides [SP02], Neutral             APCI, Chemical Synthesis, CI, EI,        Human Cell Lines, Human Primary,
University of Muenster                 Synthesis of Lipids                      glycosphingolipids [SP05], Other      Enzymatic Assays, ESI, Fluorescence
(Germany)                              (Probes/Tools/Agents), Lipid             [SP00], Phosphosphingolipids [SP03], Phosphorescence Spectroscopy, GC,




                                                                                                                                                                                                           58
                                         Metabolism, Lipid Signalling, Lipids and Sphingoid bases [SP01],                GC-MS coupling, Immune Affinity,
                                         Nutrition,                                                                      Infrared Spectroscopy, Ion Exchange,
                                                                                                                         Ion Trap, LC, LC-MS coupling, Liquid-
                                                                                                                         Liquid Extraction, Normal Phase,
                                                                                                                         Nuclear Magnetic Resonance,
                                                                                                                         Quadrupole, Reversed Phase, Size
                                                                                                                         Exclusion, Solid Phase Extraction, Thin
                                                                                                                         layer chromatography, Triple
                                                                                                                         Quadrupole, UV-Vis Spectroscopy,
Institute for Clinical Chemistry         Analytical Tools in Lipidomics,           Bile acids and derivatives [ST04],    Capillary Electrophoresis, Capillary    Biopsy, Blood, Human Cell Lines,
University of Regensburg                 Bioinformatics related to the Lipidome, Ceramides [SP02], Eicosanoids [FA03], Electrophoresis, Cell Sorting, Confocal Human Primary, Mice, Stool, Urine,
(Germany)                                Genomics of the Lipidome, Lipid           Fatty Acids and Conjugates [FA01],    Microscopy, Data Processing, Database
                                         Metabolism, Lipid Transport, Lipids and Glycerophosphocholines [GP01],          Setup, dHPLC, DNA Sequencing, EI,
                                         Nutrition, Lipids in Inflammation, Lipids Glycerophosphoethanolamines [GP02], Enzymatic Assays, ESI, Flow FRET,
                                         in Skin Care, Mapping of the Lipidome, Glycerophosphoglycerols [GP04],          Fluorescence Microscopy, GC, GC-MS
                                         Membrane Microdomains, Membrane Glycerophosphoglycerophosphates                 coupling, Gel Electrophoresis, High
                                         Trafficking,                              [GP05], Glycerophosphoserines         Troughput Microscopy, Immuno
                                                                                   [GP03], Neutral glycosphingolipids    Assays, LC, LC-MS coupling, Light
                                                                                   [SP05], Phosphosphingolipids [SP03], Microscopy, Liquid-Liquid Extraction,
                                                                                   Sphingoid bases [SP01], Steroids      Microarrays, Multicolor Analysis,
                                                                                   [ST02], Sterols [ST01],               Nuclear Magnetic Resonance, Real
                                                                                                                         Time PCR, Size Exclusion, SNP
                                                                                                                         Analysis, Thin layer chromatography,
                                                                                                                         Triple Quadrupole, Two dimensional
                                                                                                                         Electrophoresis,
Institute of Medical Physics and         Analytical Tools in Lipidomics,           Docosanoids [FA04], Fatty aldehydes Atomic Force, Electron Spin               Biopsy, Blood,
Biophysics                               Glycomics of the Lipidome, Lipids and [FA06], Glycerophosphates [GP10],         Resonance, Fluorescence Microscopy,
Medical Faculty, University of Leipzig   Nutrition, Lipids in Inflammation,        Glycerophosphocholines [GP01],        Infrared Spectroscopy, MALDI, Normal
(Germany)                                Mapping of the Lipidome, Membrane         Glycerophosphoethanolamines [GP02], Phase, Nuclear Magnetic Resonance,
                                         Microdomains,                             Glycerophosphoglycerols [GP04],       Thin layer chromatography, TOF, UV-
                                                                                   Glycerophosphoglycerophosphates       Vis Spectroscopy,
                                                                                   [GP05], Glycerophosphoinositol
                                                                                   bisphosphates [GP08],
                                                                                   Glycerophosphoinositol
                                                                                   monophosphates [GP07],
                                                                                   Glycerophosphoinositol trisphosphates
                                                                                   [GP09], Glycerophosphoinositols
                                                                                   [GP06], Glycerophosphoserines
                                                                                   [GP03], Other [GL00], Oxidized
                                                                                   glycerophospholipids [GP20],
                                                                                   Phosphosphingolipids [SP03],
                                                                                   Triradylglycerols [GL03],
LIPIDOMIX GmbH                           Analytical Tools in Lipidomics, Lipid     Docosanoids [FA04], Eicosanoids       Database Setup, ESI, LC, LC-MS          Biopsy, Blood, Human Primary, Mice,
LIPIDOMIX GmbH                           Metabolism, Lipids and Nutrition, Lipids [FA03], Fatty Acids and Conjugates     coupling, Quadrupole, Reversed Phase, Rabbits, Rats, Urine,
(Germany)                                in Inflammation, Mapping of the           [FA01], Fattyamides [FA08],           Solid Phase Extraction, Triple
                                         Lipidome, Metabolomics of the             Glycerophosphocholines [GP01],        Quadrupole,
                                         Lipidome, Nutrigenomics of the            Glycerophosphoethanolamines [GP02],
                                         Lipidome, Pharmacology and toxicology Glycerophosphoglycerols [GP04],




                                                                                                                                                                                                   59
                                          of the Lipidome,                          Glycerophosphoglycerophosphates
                                                                                    [GP05], Glycerophosphoinositol
                                                                                    bisphosphates [GP08],
                                                                                    Glycerophosphoinositol
                                                                                    monophosphates [GP07],
                                                                                    Glycerophosphoinositol trisphosphates
                                                                                    [GP09], Glycerophosphoinositols
                                                                                    [GP06], Glycerophosphoserines
                                                                                    [GP03], Octadecanoids [FA02],

Hungary

                                                                                                                                                                    Organisms and cellular Systems -
Institute                                 Areas of Lipidomics Expertise             Lipid Class - Survey                     Technology - Survey
                                                                                                                                                                    Survey
Dept. Biophysics and Cell Biology         Membrane Microdomains, Membrane                                                    Atomic Force, Cell Sorting, Confocal   Biopsy, Blood, Human Cell Lines,
University of Debrecen                    Trafficking,                                                                       Microscopy, Electron Microscopy, FLIM, Mouse, Urine, Yeast,
(Hungary)                                                                                                                    Flow FRET, Fluorescence Microscopy,
                                                                                                                             Fluorescence Phosphorescence
                                                                                                                             Spectroscopy, Gel Electrophoresis,
                                                                                                                             High Troughput Microscopy, Light
                                                                                                                             Microscopy, Multicolor Analysis, Real
                                                                                                                             Time PCR,
Laboratory of Molecular Stress Biology    Analytical Tools in Lipidomics,
Institute of Biochemistry, Biological     Genomics of the Lipidome, Lipid
Research Centre, HUng. Acad. Sci.         Metabolism, Lipid Signalling, Lipids in
(Hungary)                                 Inflammation, Mapping of the Lipidome,
                                          Membrane Microdomains,
                                          Metabolomics of the Lipidome,
                                          Pharmacology and toxicology of the
                                          Lipidome, Proteomics of the Lipidome,
                                          Signalomics of the Lipidome,
Laboratory of Nutritional Bioactivation   Analytical Tools in Lipidomics,
and Bioanalysis                           Genomics of the Lipidome, Lipid
Department of Biochemistry and            Metabolism, Lipid Signalling, Lipid
Molecular Biology, University of          Standard Bank, Lipids and Nutrition,
Debrecen                                  Lipids in Inflammation, Lipids in Skin
(Hungary)                                 Care, Metabolomics of the Lipidome,
                                          Nutrigenomics of the Lipidome,

Italy

                                                                                                                                                                      Organisms and cellular Systems -
Institute                                 Areas of Lipidomics Expertise             Lipid Class - Survey                     Technology - Survey
                                                                                                                                                                      Survey
Department of Cell Biology and            Analytical Tools in Lipidomics, Lipid     Acidic glycosphingolipids [SP06], Bile   Cell Sorting, Confocal Microscopy,       Human Cell Lines, Human Primary,
Oncology                                  Metabolism, Lipid Signalling, Lipid       acids and derivatives [ST04],            Electron Microscopy, Enzymatic           Mice, Mouse, Rabbits, Rats, Yeast,
Mario Negri Sud Institute                 Transport, Mapping of the Lipidome,       Glycerophosphocholines [GP01],           Assays, ESI, Fluorescence Microscopy,
(Italy)                                   Membrane Microdomains, Membrane           Glycerophosphoinositol bisphosphates     GC, Gel Electrophoresis, Immune
                                          Trafficking, Pharmacology and             [GP08], Glycerophosphoinositol           Affinity, Immuno Assays, Ion Exchange,




                                                                                                                                                                                                           60
                                        toxicology of the Lipidome, Proteomics monophosphates [GP07],                     LC, LC-MS coupling, Light Microscopy,
                                        of the Lipidome, Signalomics of the       Glycerophosphoinositol trisphosphates Liquid-Liquid Extraction, MALDI,
                                        Lipidome,                                 [GP09], Glycerophosphoinositolglycans Multicolor Analysis, Normal Phase,
                                                                                  [GP15], Glycerophosphoinositols [GP06], Real Time PCR, Reversed Phase, Size
                                                                                  Isoprenoids [PR01], Neutral             Exclusion, Solid Phase Extraction, Thin
                                                                                  glycosphingolipids [SP05], Sterols      layer chromatography, Two
                                                                                  [ST01],                                 dimensional Electrophoresis,
Department of Clinical and              Bioinformatics related to the Lipidome,                                                                                   Blood, Human Cell Lines, Mouse, Rats,
Experimental Medicine,                  Lipid Metabolism, Lipid Signalling, Lipid
Physiopathology, University School of   Standard Bank, Membrane
Medicine                                Microdomains, Metabolomics of the
University of Perugia                   Lipidome, Pharmacology and
(Italy)                                 toxicology of the Lipidome, Signalomics
                                        of the Lipidome,
Department of Internal Medicine,        Analytical Tools in Lipidomics, Lipid     Ceramides [SP02], Diradylglycerols      Confocal Microscopy, Enzymatic          Blood, Human Cell Lines, Rats,
Section of Biochemistry                 Metabolism, Lipid Signalling, Lipid       [GL02], Eicosanoids [FA03], Fatty Acids Assays, Fluorescence Microscopy, GC,
University of Perugia                   Transport, Lipids in Inflammation,        and Conjugates [FA01], Fatty aldehydes Gel Electrophoresis, Immuno Assays,
(Italy)                                 Mapping of the Lipidome, Membrane [FA06], Fatty esters [FA07],                    LC, Light Microscopy, Liquid-Liquid
                                        Microdomains, Membrane Trafficking, Glycerophosphates [GP10],                     Extraction, Multicolor Analysis, Normal
                                        Metabolomics of the Lipidome,             Glycerophosphocholines [GP01],          Phase, Reversed Phase, Solid Phase
                                        Pharmacology and toxicology of the        Glycerophosphoethanolamines [GP02], Extraction, Thin layer chromatography,
                                        Lipidome, Signalomics of the Lipidome, Glycerophosphoglycerols [GP04],
                                                                                  Glycerophosphoglycerophosphates
                                                                                  [GP05],
                                                                                  Glycerophosphoglycerophosphoglycerols
                                                                                  (Cardiolipins) [GP12],
                                                                                  Glycerophosphoinositols [GP06],
                                                                                  Glycerophosphoserines [GP03],
ISOF/BioFreeRadicals                    Analytical Tools in Lipidomics,           Ceramides [SP02], Docosanoids [FA04], Chemical Synthesis, EI, Electron Spin Blood, erythrocyte membranes, Human
Consiglio Nazionale delle Ricerche      Chemical Synthesis of Lipids              Eicosanoids [FA03], Fatty Acids and     Resonance, Enzymatic Assays, ESI,       Cell Lines, Tetrahymena,
(Italy)                                 (Probes/Tools/Agents), Lipid              Conjugates [FA01], Fatty esters [FA07], Fluorescence Microscopy, GC, GC-MS
                                        Metabolism, Lipid Standard Bank,          Glycerophosphocholines [GP01],          coupling, Infrared Spectroscopy, LC,
                                        Lipids and Nutrition, Lipids in           Octadecanoids [FA02],                   LC-MS coupling, Nuclear Magnetic
                                        Inflammation, Mapping of the Lipidome, Phosphosphingolipids [SP03],               Resonance, Raman Spectroscopy,
                                        Nutrigenomics of the Lipidome,            Triradylglycerols [GL03],               Reversed Phase, Solid Phase
                                                                                                                          Extraction, Thin layer chromatography,
                                                                                                                          UV-Vis Spectroscopy,

Netherlands

                                                                                                                                                                Organisms and cellular Systems -
Institute                               Areas of Lipidomics Expertise           Lipid Class - Survey                    Technology - Survey
                                                                                                                                                                Survey
Bijvoet Center                          Analytical Tools in Lipidomics,
Utrecht University                      Chemical Synthesis of Lipids
(Netherlands)                           (Probes/Tools/Agents), Glycomics of
                                        the Lipidome, Lipid Metabolism, Lipid
                                        Signalling, Lipid Transport, Membrane
                                        Microdomains, Membrane Trafficking,




                                                                                                                                                                                                    61
                                      Metabolomics of the Lipidome,
                                      Proteomics of the Lipidome,
                                      Signalomics of the Lipidome,
Institute of Biomembranes             Analytical Tools in Lipidomics,          Acidic glycosphingolipids [SP06], Basic Cell Sorting, LC, Multicolor Analysis,   Arabidopsis, Blood, Eubacteria, Human
Utrecht University                    Glycomics of the Lipidome, Lipid         glycosphingolipids [SP07],              Nuclear Magnetic Resonance, Real         Cell Lines, Mice, Yeast,
(Netherlands)                         Metabolism, Lipid Signalling, Lipid      Glycerophosphocholines [GP01],          Time PCR, Reversed Phase, Thin layer
                                      Transport, Mapping of the Lipidome,      Glycerophosphoethanolamines [GP02], chromatography,
                                      Membrane Microdomains, Membrane          Glycerophosphoserines [GP03], Neutral
                                      Trafficking,                             glycosphingolipids [SP05],
Laboratory Genetic Metabolic Diseases Analytical Tools in Lipidomics, Lipid    Bile acids and derivatives [ST04],      CI, DNA Sequencing, EI, Enzymatic        Biopsy, Blood, Human Cell Lines, Mice,
Academic Medical Center               Metabolism, Membrane Trafficking,        Ceramides [SP02], Fatty Acids and       Assays, ESI, Fluorescence Microscopy, Urine, Yeast,
(Netherlands)                         Metabolomics of the Lipidome,            Conjugates [FA01],                      GC, GC-MS coupling, Immune Affinity,
                                                                               Glycerophosphocholines [GP01],          Immuno Assays, Ion Exchange, LC,
                                                                               Glycerophosphoethanolamines [GP02], LC-MS coupling, Light Microscopy,
                                                                               Glycerophosphoglycerols [GP04],         Liquid-Liquid Extraction, nano ESI, NCI,
                                                                               Glycerophosphoglycerophosphates         Normal Phase, Quadrupole, Real Time
                                                                               [GP05],                                 PCR, Reversed Phase, Size Exclusion,
                                                                               Glycerophosphoglycerophosphoglycerols Solid Phase Extraction, Thin layer
                                                                               (Cardiolipins) [GP12],                  chromatography, Triple Quadrupole,
                                                                               Glycerophosphoinositols [GP06],         UV-Vis Spectroscopy,
                                                                               Glycerophosphoserines [GP03],
                                                                               Isoprenoids [PR01],
Nutrition, metabolism and genomics    Analytical Tools in Lipidomics,                                                  Biostatistics, Cluster Analysis, Data
group                                 Bioinformatics related to the Lipidome,                                          Processing, Database Setup,
Wageningen University                 Genomics of the Lipidome, Lipid                                                  Microarrays, Real Time PCR,
(Netherlands)                         Metabolism, Lipid Signalling, Lipids and
                                      Nutrition, Lipids in Inflammation,
                                      Nutrigenomics of the Lipidome,

                                                                                                                                                              Organisms and cellular Systems -
Institute                              Areas of Lipidomics Expertise          Lipid Class - Survey                    Technology - Survey
                                                                                                                                                              Survey
Institute of Biophysics, Faculty of    Membrane Microdomains, Membrane        Glycerophosphocholines [GP01],        Fluorescence Microscopy, Light            Human Cell Lines,
Medicine                               Trafficking,                           Glycerophosphoserines [GP03],         Microscopy,
University of Ljubljana                                                       Phosphosphingolipids [SP03], Steroids
(Slovenia)                                                                    [ST02],

Spain

                                                                                                                                                            Organisms and cellular Systems -
Institute                             Areas of Lipidomics Expertise           Lipid Class - Survey                    Technology - Survey
                                                                                                                                                            Survey
Institute of Molecular Biology and    Lipid Metabolism, Lipid Signalling,     Diradylglycerols [GL02], Eicosanoids Capillary Electrophoresis, Cell Sorting, Blood, human, Human Cell Lines,
Genetics                              Lipids in Inflammation, Mapping of the [FA03], Fatty Acids and Conjugates    Confocal Microscopy, DNA                 Human Primary, Mice, Mouse, Rabbits,
Spanish Research Council & University Lipidome, Pharmacology and              [FA01], Glycerophosphates [GP10],    Sequencing, Enzymatic Assays,            Rats,
of Valladolid School of Medicine      toxicology of the Lipidome, Signalomics Glycerophosphocholines [GP01],       Fluorescence Microscopy, GC, Gel
(Spain)                               of the Lipidome,                        Glycerophosphoethanolamines [GP02], Electrophoresis, Immune Affinity,
                                                                              Glycerophosphoglycerols [GP04],      Immuno Assays, Ion Exchange, LC,
                                                                              Glycerophosphoglycerophosphates      Light Microscopy, Liquid-Liquid
                                                                              [GP05],                              Extraction, Multicolor Analysis, Normal



                                                                                                                                                                                                  62
                                                                            Glycerophosphoglycerophosphoglycerols Phase, Real Time PCR, Reversed
                                                                            (Cardiolipins) [GP12],                  Phase, Size Exclusion, Solid Phase
                                                                            Glycerophosphoinositol bisphosphates Extraction, Thin layer chromatography,
                                                                            [GP08], Glycerophosphoinositol          Two dimensional Electrophoresis,
                                                                            monophosphates [GP07],
                                                                            Glycerophosphoinositol trisphosphates
                                                                            [GP09], Glycerophosphoinositols [GP06],
                                                                            Glycerophosphoserines [GP03],
                                                                            Glyceropyrophosphates [GP11],
                                                                            Monoradylglycerols [GL01],
                                                                            Triradylglycerols [GL03],

Switzerland

                                                                                                                                                           Organisms and cellular Systems -
Institute                         Areas of Lipidomics Expertise             Lipid Class - Survey                  Technology - Survey
                                                                                                                                                           Survey
Division of Biochemistry          Analytical Tools in Lipidomics,           Ceramides [SP02], Fatty Acids and        Confocal Microscopy, ESI,             Yeast, yeast,
University of Fribourg            Genomics of the Lipidome, Lipid           Conjugates [FA01],                       Fluorescence Microscopy, GC, Gel
(Switzerland)                     Metabolism, Lipid Signalling, Lipid       Glycerophosphoglucose lipids [GP14], Electrophoresis, Light Microscopy, Thin
                                  Transport, Lipids in Microbiology,        Glycerophosphoglycerophosphoglycerols layer chromatography,
                                  Mapping of the Lipidome, Membrane         (Cardiolipins) [GP12],
                                  Microdomains, Membrane Trafficking,       Glycerophosphoinositolglycans [GP15],
                                  Proteomics of the Lipidome,               Glycerophosphoinositols [GP06],
                                                                            Phosphosphingolipids [SP03], Sphingoid
                                                                            bases [SP01], Steroid conjugates [ST05],
                                                                            Steroids [ST02], Sterols [ST01],

United Kingdom

                                                                                                                                                           Organisms and cellular Systems -
Institute                         Areas of Lipidomics Expertise             Lipid Class - Survey                  Technology - Survey
                                                                                                                                                           Survey
European application laboratory   Analytical Tools in Lipidomics,                                                 APCI, APPI, Biostatistics, CI, Cluster
Thermo Electron                                                                                                   Analysis, Data Processing, Database
(United Kingdom)                                                                                                  Setup, EI, Enzymatic Assays, ESI,
                                                                                                                  FTICR, GC-MS coupling, Gel
                                                                                                                  Electrophoresis, Infrared Spectroscopy,
                                                                                                                  Ion Trap, LC, LC-MS coupling, MALDI,
                                                                                                                  nano ESI, NCI, Orbitrap, Quadrupole,
                                                                                                                  Raman Spectroscopy, Reversed
                                                                                                                  Phase, Triple Quadrupole, UV-Vis
                                                                                                                  Spectroscopy,
Glycobiology Institute            Analytical Tools in Lipidomics,                                                 Cell Sorting, Chemical Synthesis,       Biopsy, Blood, Human Cell Lines,
Oxford University                 Bioinformatics related to the Lipidome,                                         Enzymatic Assays, Fluorescence          Human Primary, Mice, Mouse, Urine,
(United Kingdom)                  Chemical Synthesis of Lipids                                                    Microscopy, Ion Exchange, LC, LC-MS
                                  (Probes/Tools/Agents), Glycomics of                                             coupling, Light Microscopy, MALDI,
                                  the Lipidome, Lipid Metabolism, Lipid                                           Multicolor Analysis, Normal Phase,
                                  Signalling, Lipid Transport, Lipids in                                          Nuclear Magnetic Resonance,
                                  Inflammation, Lipids in Microbiology,                                           oligosaccharide profiling, QqTOF, Real




                                                                                                                                                                                              63
                               Mapping of the Lipidome, Membrane                                               Time PCR, Reversed Phase, Size
                               Microdomains, Membrane Trafficking,                                             Exclusion, Solid Phase Extraction, Thin
                               Metabolomics of the Lipidome,                                                   layer chromatography, TOF,
                               Pharmacology and toxicology of the
                               Lipidome, Proteomics of the Lipidome,
Institute for Cancer Studies   Analytical Tools in Lipidomics,          Ceramides [SP02], Diradylglycerols      APCI, APPI, Capillary Electrophoresis, Biopsy, Blood, Human Cell Lines,
University of Birmingham       Bioinformatics related to the Lipidome,  [GL02], Eicosanoids [FA03], Fatty Acids CI, Confocal Microscopy, dHPLC, DNA Human Primary, Mice, Mouse,
(United Kingdom)               Genomics of the Lipidome, Lipid          and Conjugates [FA01], Fatty alcohols Sequencing, EI, Enzymatic Assays,
                               Metabolism, Lipid Signalling, Lipid      [FA05], Glycerophosphates [GP10],       ESI, Fluorescence Microscopy, FTICR,
                               Transport, Lipids in Inflammation,       Glycerophosphocholines [GP01],          GC, GC-MS coupling, Gel
                               Mapping of the Lipidome, Membrane        Glycerophosphoethanolamines [GP02], Electrophoresis, Immuno Assays, Ion
                               Microdomains, Membrane Trafficking,      Glycerophosphoglycerols [GP04],         Exchange, Ion Trap, LC, LC-MS
                               Metabolomics of the Lipidome,            Glycerophosphoglycerophosphates         coupling, Light Microscopy, MALDI,
                               Pharmacology and toxicology of the       [GP05], Glycerophosphoinositol          Microarrays, nano ESI, NCI, Normal
                               Lipidome, Proteomics of the Lipidome,    bisphosphates [GP08],                   Phase, Nuclear Magnetic Resonance,
                               Signalomics of the Lipidome,             Glycerophosphoinositol                  Quadrupole, Real Time PCR, Reversed
                                                                        monophosphates [GP07],                  Phase, Size Exclusion, Solid Phase
                                                                        Glycerophosphoinositol trisphosphates Extraction, Surface Plasmon
                                                                        [GP09], Glycerophosphoinositols [GP06], Resonance, Thin layer
                                                                        Glycerophosphoserines [GP03],           chromatography, TOF, Triple
                                                                        Monoradylglycerols [GL01], Oxidized     Quadrupole, Two dimensional
                                                                        glycerophospholipids [GP20],            Electrophoresis, UV-Vis Spectroscopy,
                                                                        Phosphosphingolipids [SP03], Sphingoid
                                                                        bases [SP01],
Mass Spectrometry Facility     Analytical Tools in Lipidomics, Lipid    Bile acids and derivatives [ST04],      ESI, Gel Electrophoresis, Ion           Biopsy, Blood, Human Cell Lines, Mice,
The School of Pharmacy         Metabolism, Lipid Signalling, Lipids and Isoprenoids [PR01], Polyprenols [PR03], Exchange, Ion Trap, LC, LC-MS           Mouse, Rats, Urine,
(United Kingdom)               Nutrition, Mapping of the Lipidome,      Secosteroids [ST03], Steroid conjugates coupling, MALDI, nano ESI, Normal
                               Membrane Microdomains, Membrane [ST05], Steroids [ST02], Sterols [ST01], Phase, Nuclear Magnetic Resonance,
                               Trafficking, Metabolomics of the                                                 QqTOF, Quadrupole, Reversed Phase,
                               Lipidome, Pharmacology and                                                       Size Exclusion, Solid Phase Extraction,
                               toxicology of the Lipidome, Proteomics                                           TOF, Triple Quadrupole,
                               of the Lipidome, Signalomics of the
                               Lipidome,
School of Chemistry            Analytical Tools in Lipidomics,          CDP-Glycerols [GP13], Ceramides         Atomic Force, Biostatistics, Cell       Biopsy, Blood, Human Cell Lines,
University of Southampton      Bioinformatics related to the Lipidome, [SP02], Diradylglycerols [GL02],         Sorting, Chemical Synthesis, Cluster    Human Primary, Mice, Yeast,
(United Kingdom)               Chemical Synthesis of Lipids             Docosanoids [FA04], Eicosanoids         Analysis, Confocal Microscopy, Data
                               (Probes/Tools/Agents), Glycomics of      [FA03], Fatty aldehydes [FA06],         Processing, Database Setup, DNA
                               the Lipidome, Lipid Metabolism, Lipid Glycerophosphocholines [GP01],             Sequencing, Electron Microscopy,
                               Signalling, Lipid Transport, Lipids and Glycerophosphoethanolamines [GP02], Electron Spin Resonance, Enzymatic
                               Nutrition, Lipids in Inflammation,       Glycerophosphoglycerols [GP04],         Assays, ESI, Fluorescence Microscopy,
                               Mapping of the Lipidome, Membrane Glycerophosphoglycerophosphates                FTICR, Gel Electrophoresis, Immuno
                               Microdomains, Membrane Trafficking, [GP05],                                      Assays, Infrared Spectroscopy, LC, LC-
                               Metabolomics of the Lipidome,            Glycerophosphoglycerophosphoglycerols MS coupling, Light Microscopy, Liquid-
                               Nutrigenomics of the Lipidome,           (Cardiolipins) [GP12],                  Liquid Extraction, Microarrays,
                               Pharmacology and toxicology of the       Glycerophosphoinositol bisphosphates Multicolor Analysis, nano ESI, Near
                               Lipidome, Signalomics of the Lipidome, [GP08], Glycerophosphoinositol            Field, Normal Phase, Nuclear Magnetic
                                                                        monophosphates [GP07],                  Resonance, QqTOF, Quadrupole,
                                                                        Glycerophosphoinositols [GP06],         Raman Spectroscopy, Real Time PCR,




                                                                                                                                                                                           64
                                                                           Glycerophosphoserines [GP03],           Reversed Phase, SNP Analysis, Solid
                                                                           Oxidized glycerophospholipids [GP20],   Phase Extraction, Surface Plasmon
                                                                           Oxygenated hydrocarbons [FA12],         Resonance, Triple Quadrupole, UV-Vis
                                                                           Phosphosphingolipids [SP03],            Spectroscopy,
                                                                           Triradylglycerols [GL03],

United States

                                                                                                                                                            Organisms and cellular Systems -
Institute                          Areas of Lipidomics Expertise           Lipid Class - Survey                    Technology - Survey
                                                                                                                                                            Survey
Department of Biochemistry         Lipid Metabolism, Lipid Signalling,     Diradylglycerols [GL02], Docosanoids Biostatistics, Cell Sorting, Cluster        Archaebacteria, Biopsy, Blood,
Wake Forest University School of   Lipids and Nutrition, Lipids in         [FA04], Eicosanoids [FA03], Fatty Acids Analysis, Confocal Microscopy, Data      Eubacteria, Human Cell Lines, Human
Medicine                           Inflammation, Lipids in Microbiology,   and Conjugates [FA01], Fatty ethers     Processing, Database Setup, DNA          Primary, Mice, Mouse, Rabbits, Rats,
(United States)                    Signalomics of the Lipidome,            [FA10], Glycerophosphates [GP10],       Sequencing, Electron Microscopy,         Stool, Urine, Yeast,
                                                                           Glycerophosphoinositol bisphosphates Electron Spin Resonance, Enzymatic
                                                                           [GP08], Glycerophosphoinositol          Assays, Fluorescence Microscopy, GC,
                                                                           monophosphates [GP07],                  Gel Electrophoresis, Immune Affinity,
                                                                           Glycerophosphoinositol trisphosphates Immuno Assays, Infrared Spectroscopy,
                                                                           [GP09], Glycerophosphoinositols         Ion Exchange, LC, Light Microscopy,
                                                                           [GP06],                                 Liquid-Liquid Extraction, Microarrays,
                                                                                                                   Multicolor Analysis, Normal Phase,
                                                                                                                   Nuclear Magnetic Resonance, Raman
                                                                                                                   Spectroscopy, Real Time PCR,
                                                                                                                   Reversed Phase, Size Exclusion, SNP
                                                                                                                   Analysis, Thin layer chromatography,
                                                                                                                   Two dimensional Electrophoresis, UV-
                                                                                                                   Vis Spectroscopy,




                                                                                                                                                                                              65
         Appendix 3: Contact details of user registered in LEP ordered by country and organisation (as per 01.01.2008)

Lastname        Firstname      Email                               Organisation              Company                    Phone              ZIP      City         Street               Country
Barrantes       Francisco J.   rtfjb1@criba.edu.ar                 Universidad Nacional del UNESCO Chair                054 2914861201     8000     Bahia Blanca C.C. 857             Argentina
                                                                   Sur                      Biophys. & Mol.
                                                                                            Neurobiol
muller          maria          vivimico@yahoo.com.br               Universidad Nacional del UNESCO Chair                054 2914861201     8000     Bahia Blanca C.C. 857             Argentina
                                                                   Sur                      Biophys. & Mol.
                                                                                            Neurobiol
Lohner          Karl           karl.lohner@oeaw.ac.at              Austrian Academy of      Inst. Biophysics & X-ray    ++43-316-4120-     A-8045   Graz         Schmiedlstrasse 6    Austria
                                                                   Sciences                 Structure Research /        323
                                                                                            Fct. Lipidomics
Prassl          Ruth           ruth.prassl@oeaw.ac.at              Austrian Academy of       Inst. Biophysics & X-ray   ++43-316-4120-     A-8045   Graz         Schmiedlstrasse 6    Austria
                                                                   Sciences                  Structure Research /       323
                                                                                             Fct. Lipidomics
Weinberger      Klaus          klaus.weinberger@biocrates.com      BIOCRATES Life            BIOCRATES Life             ++43-512-579823-   6020     Innsbruck    Innrain 66           Austria
                                                                   Sciences                  Sciences                   4216
Hackl           Hubert         hubert.hackl@tugraz.at              Graz University of        Institute for Genomics     ++43-316-873-      8010     Graz         Petersgasse 14       Austria
                                                                   Technology                and Bioinformatics         5345
Pichler         Harald         harald.pichler@tugraz.at            Graz University of        Institute for Molecular    ++43-316-873-      A-8010   Graz         Petersgasse 14/I     Austria
                                                                   Technology                Biotechnology              4089
Daum            Guenther       guenther.daum@tugraz.at             Graz University of        Institute of Biochemistry, +43-316-873-6462   A-8010   Graz         Petersgasse 12/2     Austria
                                                                   Technology                Cell Biology Group
Keller          Walter         walter.keller@uni-graz.at           Inst. of Chemistry        Structural biology         +43-316-3805423    8010     Graz         Heinrichstr. 28      Austria
Sinner          Frank          frank.sinner@joanneum.at            Joanneum Research         Institute of Medical       ++433168762103     8036     Graz         Auenbruggerplatz     Austria
                                                                                             Technologies and                                                    20/3
                                                                                             Health Mangement
Kostner         Gert,M.        gerhard.kostner@meduni-graz.at      Medical University Graz   Molecular Biology and      +433163804200      A-8010   Graz         Harrachgasse 21      Austria
                                                                                             Biochemistry,
Frank           Sasa           sasa.frank@meduni-graz.at           Medical University Graz   Molecular Biology and      +433163804200      A-8010   Graz         Harrachgasse 21      Austria
                                                                                             Biochemistry,
Kohlwein        Sepp D.        sepp.kohlwein@uni-graz.at           University of Graz        IMB Biochemistry -         ++43 316 380       A8010    Graz         Schubertstr. 1       Austria
                                                                                             Yeast Genetics Group       5487
Allmaier        Guenter        guenter.allmaier@tuwien.ac.at       Vienna University of      Institute of Chemical      ++43 1 58801       A-1060   Vienna       Getreidemarkt        Austria
                                                                   Technology                Technologies and           15160                                    9/164
                                                                                             Analytics, Bio and
                                                                                             Polymer Analysis

Köfeler         Harald         harald.koefeler@klinikum-graz.at    ZMF/Medical University    Core Facility for Mass     ++43 (316) 385-    8010     Graz         Stiftingtalstrasse   Austria
                                                                   Graz                      Spectrometry               73005                                    24
Van Veldhoven   Paul           paul.vanveldhoven@med.kuleuven.be   K.U.Leuven                Div. Pharmacology -        ++32-16-345801     B-3000   Leuven       Campus               Belgium
                                                                                             Dep. Mol. Cell Biology                                              Gasthuisberg,
                                                                                                                                                                 Herestraat
                                                                                                                                                                   ELIfe Final Report

Thevissen      Karin        karin.thevissen@biw.kuleuven.be   Katholieke Universiteit     CMPG-PFI                   ++32 16 32 96 88    3001       Heverlee       Kasteelpark           Belgium
                                                              Leuven                                                                                               Arenberg 20
roelants       ivo          ivo.roelants@lrd.kuleuven.be      Katholieke Universiteit     CMPG-PFI                   ++32 16 32 96 88    3001       Heverlee       Kasteelpark           Belgium
                                                              Leuven                                                                                               Arenberg 20
Swinnen        Johannes     johan.swinnen@med.kuleuven.be     Katholieke Universiteit     Laboratory for             ++32-16-330533      3000       Leuven         Herestraat 49 bus     Belgium
                                                              Leuven                      Experimental Medicine                                                    902
                                                                                          and Endocrinology
                                                                                          (LEGENDO)

Dotti          Carlos       carlos.dotti@med.kuleuven.be      VIB and Catholic            Neuronal Differentiation   +32-16-330526       3000       Leuven         Herestraat 49         Belgium
                                                              University of Leuven        Unit
Trindade       Vera Maria   vmtt@ufrgs.br                     Departamento de             Laboratório de             55-51-33-16-55-50   90.035-    Porto Alegre   Rua Ramiro            Brazil
               Treis                                          Bioquímica - ICBS-          Bioquímica e Biologia                          003                       Barcelos 2600-
                                                              Universidade Federal do     Celular de Lipídios                                                      anexo
                                                              rio Grande do Sul

Boggs          Joan         jmboggs@sickkids.ca               Hospital for Sick           Research Institute,        1-416-813-5919      M5G1X8     Toronto        555 University Ave. Canada
                                                              Children                    Boggs Laboratory
Lehner         Richard      richard.lehner@ualberta.ca        University of Alberta       CIHR Group in              1-780-492-2963      T6G 2S2    Edmonton       328 Heritage          Canada
                                                                                          Molecular and Cell                                                       Medical Research
                                                                                          Biology of Lipids                                                        Centre
Hu             Zanmin       zmhu@genetics.ac.cn               Institute of Genetics and   Lab 305                    86-10-64889783      100101     Beijing        Datun                 China
                                                              Developmental Biology,                                                                               Road,Chaoyang
                                                              Chinese Academy of                                                                                   District
                                                              Sciences

Mu             Huiling      hm@biocentrum.dtu.dk              The Technical University    Biochemistry and           +45 45252744        2800       Lyngby         Building 224, DTU     Denmark
                                                              of Denmark                  Nutrition Group,
                                                                                          Biocentrum-DTU
Yki-Järvinen   Hannele      ykijarvi@cc.helsinki.fi           Department of Medicine      University of Helsinki     +358 50 563 6899    00020      Helsinki       PO Box 700            Finland
Kinnunen       Paavo        paavo.kinnunen@helsinki.fi        Institute of Biomedicine    Helsinki Biophysics &      +358-9-19125400     FIN-       Helsinki       POB 63                Finland
                                                                                          Biomembrane Group                              00014
                                                                                                                                         University
                                                                                                                                         of
                                                                                                                                         Helsinki
Käkelä         Reijo        reijo.kakela@helsinki.fi          University of Helsinki      Institute of               ++358-9-            P.O. Box Helsinki         Haartmaninkatu        Finland
                                                                                          Biomedicine/Käkelä         19125409            63                        8/P.O. Box 63
                                                                                          Group
Somerharju     Pentti       pentti.somerharju@helsinki.fi     University of Helsinki      Institute of           358-9-19125410          00014      Helsinki       Haartmaninkatu        Finland
                                                                                          Biomedicine/Somerharju                                                   8/PL 63
                                                                                          group
Hiltunen       J. Kalervo   kalervo.hiltunen@oulu.fi          University of Oulu          Department of              +358-8-553 1150     FIN-       Oulu           POB 3000              Finland
                                                                                          Biochemisty                                    90014
Oresic         Matej        matej.oresic@vtt.fi               VTT Technical Research Quantitative Biology and ++358-20-720-              FIN-       Espoo          Tietotie 2, P.O. Box Finland
                                                              Centre of Finland      Bioinformatics           4491                       02044                     1500
                                                                                                                                         VTT


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                                                                                                                                                            ELIfe Final Report

Chardot    Thierry        thierry.chardot@grignon.inra.fr       Biological Chemistry      Biological Chemistry      ++33-1-30815474     78850   Thiverval   BP 1                     France
                                                                LaboratoryNational        Laboratory                                            Grignon
                                                                Institute for Agronomic
                                                                Research (INRA)

Colard     Odile          colard@ccr.jussieu.fr                 CHU Saint-Antoine         INSERM-538                33-01-40011340      75571   PARIS 12°   27 rue Chaligny          France
                                                                                          (biomembranes)
Carriere   Frederic       carriere@ibsm.cnrs-mrs.fr             CNRS                      Laboratory of             33 4 91 16 41 34    13402   Marseille   31 chemin Joseph         France
                                                                                          Enzymology at                                         cedex 20    Aiguier
                                                                                          Interfaces and
                                                                                          Physiology of Lipolysis

CHIMINI    Giovanna       chimini@ciml.univ-mrs.fr              CNRS INSERM               CIML                      33 491269404        13288   marseille   parc sxcientific de      France
                                                                                                                                                            luminy
He         Hai-Tao        he@ciml.univ-mrs.fr                   CNRS INSERM               CIML                      33 491269404        13288   marseille   parc sxcientific de      France
                                                                                                                                                            luminy
Lessire    René           rene.lessire@biomemb.u-bordeaux2.fr   CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                                                                Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
LESSIRE    René           rene.lessire@biomemb.u-bordeaux2.fr   CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                                                                Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
Moreau     Patrick        pmoreau@biomemb.u-bordeaux2.fr        CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                                                                Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
MANETA-    Lilly          lilly.maneta-peyret@biomemb.u-        CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
PEYRET                    bordeaux2.fr                          Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
bessoule   jean-jacques   jean-jacques.bessoule@biomemb.u-      CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                          bordeaux2.fr                          Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
Domergue   Frédéric       frederic.domergue@u-bordeaux2.fr      CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                                                                Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
JEROME     JOUBES         jjoubes@biomemb.u-bordeaux2.fr        CNRS UMR 5200             Laboratoire de        +33 (0)5 57 57 10       33076   Bordeaux    146 Rue Léo              France
                                                                Université V. Segalen     Biogenèse Membranaire 45                              cedex       Saignat
                                                                Bordeaux 2
Lambeau    Gerard         lambeau@ipmc.cnrs.fr                  CNRS UMR6097              Institute of molecular    ++33-(0)4-          06560   Valbonne    Sophia Antipolis-        France
                                                                                          and cellular              93957733                                660 route des
                                                                                          pharmacology                                                      lucioles
Edelman    Aleksander     edelman@necker.fr                     Faculté de Médecine       INSERM U467/IFR94         +33 1 40 61 56 21   75015   Paris       156 rue de               France
                                                                René Descartes Paris 5    Proteomics Platform                                               Vaugirard
Ollero     Mario          ollero@necker.fr                      Faculté de Médecine       INSERM U467/IFR94         +33 1 40 61 56 21   75015   Paris       156 rue de               France
                                                                René Descartes Paris 5    Proteomics Platform                                               Vaugirard



                                                                                                                                                                                68
                                                                                                                                                                            ELIfe Final Report

STAAT             Frédéric       f.staat@iterg.com                      Industrial Technical       French Institute for Fats   +33 5 56 36 00 44   33600     PESSAC         rue Monge                France
                                                                        Center                     and Oils (ITERG)
LAGARDE           Michel         Michel.Lagarde@insa-lyon.fr            INSA-Lyon and CNRS /       IMBL-Lipid Signaling        33-4-72 43 82 40    69621     Villeurbanne   20 Ave A. Einstein       France
                                                                        INSERM / Univ Lyon1        (UMR 585 INSERM /
                                                                                                   INSA-Lyon)
GUICHARDANT Michel               Michel.Guichardant@insa-lyon.fr        INSA-Lyon and CNRS /       IMBL-Lipid Signaling        33-4-72 43 82 40    69621     Villeurbanne   20 Ave A. Einstein       France
                                                                        INSERM / Univ Lyon1        (UMR 585 INSERM /
                                                                                                   INSA-Lyon)
Dugail            Isabelle       idugail@bhdc.jussieu.fr                INSERM                     INSERM U671                 ++33142346923       75006     Paris          15 rue de l ecole        France
                                                                                                                                                                            de medecine
Saulnier-Blache   Jean           saulnier@toulouse.inserm.fr            INSERM,                    INSERM U586 (Obesity        33-5-62172956       31432     Toulouse       CHU Rangueil             France
                  Sébastien                                                                        Research Unit)                                                           BP84225
Bertrand-Michel   Justine        justine.Bertrand-                      INSERM/Toulouse            IFR30 Lipid Analysis        33 (0)561779414     31024     Toulouse       CPTP Bat C,              France
                                 Michel@toulouse.inserm.fr              Genopole                   Platform                                                                 Hopital Purpan
                                                                                                                                                                            BP3028
Nicaud            Jean-marc      jean-marc.nicaud@grignon.inra.fr       National Institute for     Microbiology and            ++33 130815450      F78850    Thiverval-     CBAI B.P. 01             France
                                                                        Agronomic Research         Molecular Genetic                                         Grignon
                                                                        (INRA) and National        Laboratory, UMR2585
                                                                        Centre for Scientific
                                                                        Research (CNRS)

Leray             Claude         leraycl@wanadoo.fr                     Private                    Cyberlipid Center           330467414365        34090     Montpellier    2 rue du colonel         France
                                                                                                                                                                            Marchand
Bougnoux          Philippe       bougnoux@med.univ-tours.fr             Université François        Nutrition, Croissance et    +33 (0)247 366179 37044       TOURS          CHU Bretonneau           France
                                                                        Rabelais                   Cancer, INSERM E211
Khan              Naim           Naim.Khan@u-bourgogne.fr               University                 University of Bourgogne     +33 3 80 39 63 12   21000     DIJON          6 Bd Gabriel             France

Vidal-Wagner      Juan           Juan.Vidal@eur.appliedbiosystems.com   Applera Deutschland        Applied Biosystems          +49615196705231     64293     Darmstadt      Frankfurter Strasse Germany
                                                                        GmbH                                                                                                129B
Berthold          Heiner         hkb@akdae.de                           Arzneimittelkommission     AkdÄ                        ++4930400456500 10623         Berlin         Herbert-Lewin-           Germany
                                                                        der deutschen                                                                                       Platz 1
                                                                        Ärzteschaft
Mueller           Martin J.      Martin.Mueller@biozentrum.uni-         Biocenter, University of   Julius-v-Sachs-Institute    ++49-931-888-       D-97082   Wuerzburg      Julius-von-Sachs-        Germany
                                 wuerzburg.de                           Wuerzburg                  for Biosciences,            6160                                         Platz 2
                                                                                                   Pharmaceutical Biology
Krieg             Peter          p.krieg@dkfz.de                        Deutsches              Eicosanoids and Tumor           49 6221 424506      D 69120   Heidelberg     Im Neuenheimer           Germany
                                                                        Krebsforschungszentrum Development                                                                  Feld 280
Kleinhammer       Christian      c.kleinhammer@t-online.de              DGPF                       Deutsche Gesellschaft       089-1897 9007       82152     Martinsried    Am Klopferspitz 18       Germany
                                                                                                   für Proteomforschung
Gröne             Hermann-Josef h.-j.groene@dkfz.de                     DKFZ(German Cancer         department of cellular  49-6221-424350          D-69120   Heidelberg     Im Neuenheimer           Germany
                                                                        Research Center)           and molecular pathology                                                  Feld 280
Beck              Renata         majussia@gmail.com                     DKFZ(German Cancer         department of cellular  49-6221-424350          D-69120   Heidelberg     Im Neuenheimer           Germany
                                                                        Research Center)           and molecular pathology                                                  Feld 280


                                                                                                                                                                                                69
                                                                                                                                                      ELIfe Final Report

Bernhard    Wolfgang   wolfgang.bernhard@med.uni-         Eberhard Karls            Dept. of Neonatology,   #49-(0)7071-29-    72076     Tübingen     Calwerstr. 7            Germany
                       tuebingen.de                       Universität Tübingen      Lipid Metabolism and    86377
                                                                                    Developmental
                                                                                    Physiology Group

Bernhard    Wolfgang   wolfgang.bernhard@med.uni-         Eberhard Karls            Dept. of Neonatology,   #49-(0)7071-29-    72076     Tübingen     Calwerstr. 7            Germany
                       tuebingen.de                       Universität Tübingen      Lipid Metabolism and    86377
                                                                                    Developmental
                                                                                    Physiology Group

Gesche      Jens       jgesche@web.de                     Eberhard Karls            Dept. of Neonatology,   #49-(0)7071-29-    72076     Tübingen     Calwerstr. 7            Germany
                                                          Universität Tübingen      Lipid Metabolism and    86377
                                                                                    Developmental
                                                                                    Physiology Group

Feussner    Ivo        ifeussn@uni-goettingen.de          Georg-August-University   Albrecht-von-Haller-    ++49-551-39-5743   37077     Goettingen   Justus-von-Liebig       Germany
                                                                                    Institute for Plant                                               Weg 11
                                                                                    Sciences/Plant
                                                                                    Biochemistry

Göbel       Cornelia   cgoebel@uni-goettingen.de          Georg-August-University   Albrecht-von-Haller-    ++49-551-39-5743   37077     Goettingen   Justus-von-Liebig       Germany
                                                                                    Institute for Plant                                               Weg 11
                                                                                    Sciences/Plant
                                                                                    Biochemistry

Willumeit   Regine     regine.willumeit@gkss.de           GKSS Research Center      Department of           ++49-4152-87-      21502     Geesthacht   Max-Planck-Str. 1       Germany
                                                                                    Macromolecular          1291
                                                                                    Structure Research
Herrmann    Andreas    andreas.herrmann@rz.hu-berlin.de   Humboldt-University       Institute of            49 (0) 30 2093     D-10115   Berlin       Invalidenstr. 42        Germany
                                                          Berlin                    Biology/Molecular       8860
                                                                                    Biophysics
Rosenau     Frank      f.rosenau@fz-juelich.de            Institute for Molecular   Microbial Expression    +492461612947      52426     Juelich      Stetternicher Forst     Germany
                                                          Enzyme Technology,        Technology
                                                          Heinrich-Heine
                                                          University Duesseldorf

Kovacic     Filip      f.kovacic@fz-juelich.de            Institute for Molecular   Microbial Expression    +492461612947      52426     Juelich      Stetternicher Forst     Germany
                                                          Enzyme Technology,        Technology
                                                          Heinrich-Heine
                                                          University Duesseldorf

Kovacic     Filip      f.kovacic@fz-juelich.de            Institute for Molecular   Microbial Expression    +492461612947      52426     Juelich      Stetternicher Forst     Germany
                                                          Enzyme Technology,        Technology
                                                          Heinrich-Heine
                                                          University Duesseldorf




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                                                                                                                                                                  ELIfe Final Report

Hausmann      Sascha    S.Hausmann@fz-juelich.de                  Institute for Molecular   Microbial Expression        +492461612947      52426     Juelich      Stetternicher Forst      Germany
                                                                  Enzyme Technology,        Technology
                                                                  Heinrich-Heine
                                                                  University Duesseldorf

Rothe         Michael   michael.rothe@lipidomix.de                LIPIDOMIX GmbH            LIPIDOMIX GmbH              ++49-30-           D-13088   Berlin       Berliner Allee 261-      Germany
                                                                                                                        76778940                                  269
Adam          Olaf      olaf.adam@lrz.uni-muenchen.de             Ludwig-Maximilians-       Walther-Straub-Institute    +49-89-21807564    80336     Munich       Goethestr. 33            Germany
                                                                  University
Doermann      Peter     doermann@mpimp-golm.mpg.de                Max Planck Society        Max Planck Institute of     ++49-331-567-      14476     Golm         Am Mühlenberg 1          Germany
                                                                                            Molecular Plant             8259
                                                                                            Physiology/Plant Lipid
                                                                                            Group

Schiller      Jürgen    Juergen.Schiller@medizin.uni-leipzig.de   Medical Faculty,          Institute of Medical        ++49-341-          04107     Leipzig      Härtelstrasse 16-        Germany
                                                                  University of Leipzig     Physics and Biophysics      9715733                                   18
Zschörnig     Olaf      zsco@medizin.uni-leipzig.de               Medical Faculty,          Institute of Medical        ++49-341-          04107     Leipzig      Härtelstrasse 16-        Germany
                                                                  University of Leipzig     Physics and Biophysics      9715733                                   18
Frentzen      Margrit   frentzen@bio1.rwth-aachen.de              RWTH Aachen               Institute for Biology I,    +49-(0)241-        52074     Aachen       Worringerweg 1           Germany
                                                                  University                Botany                      8026641
Sadre         Radin     rsadre@bio1.rwth-aachen.de                RWTH Aachen               Institute for Biology I,    +49-(0)241-        52074     Aachen       Worringerweg 1           Germany
                                                                  University                Botany                      8026641
Reinartz      Andrea    andrea.reinartz@rwth-aachen.de            RWTH-Aachen               Institute for Pathology,    ++492418089729     52074     Aachen       Pauwelstr. 30            Germany
                                                                                            University Hospital
                                                                                            Áachen
Sandhoff      Konrad    sandhoff@uni-bonn.de                      Universität Bonn          Kekulé Institut f.    ++49 228 735346          53121     Bonn         Gerhard-Domagk-          Germany
                                                                                            Organische Chemie und                                                 Straße 1
                                                                                            Biochemie
van Echten-   Gerhild   g.echten.deckert@uni-bonn.de              University Bonn           Kekulé-Institute for        ++49-228-732703    53121     Bonn         Gerhard-Domagk-          Germany
Deckert                                                                                     Organic Chemistry and                                                 Str. 1
                                                                                            Biochemistry
Füllekrug     Joachim   Joachim.Fuellekrug@med.uni-               University Heidelberg     Department of               (+49) 6221         69120     Heidelberg   INF 410                  Germany
                        heidelberg.de                                                       Gastroenterology            5638960
Ceglarek      Uta       uta.ceglarek@medizin.uni-leipzig.de       University Hospital       Insitute of Laboratory      ++49-341-          04103     Leipzig      Liebigstraße 27          Germany
                                                                  Leipzig                   Medicine, Clinical          9722407
                                                                                            Chemistry and
                                                                                            Molecular Diagnostics

Höning        Stefan    shoening@uni-koeln.de                     University of Cologne     Institute of Biochemistry   ++49-221-          50931     Cologne      Joseph-Stelzmann- Germany
                                                                                            I                           4783656                                   Str. 52
Sperling      Petra     sperling@botanik.uni-hamburg.de           University of Hamburg     Biocenter Klein             +49-(0)40-42816-   D-22609   Hamburg      Ohnhorststr. 18          Germany
                                                                                            Flottbekand Botanical       343
                                                                                            Garden/Plant
                                                                                            Physiology



                                                                                                                                                                                      71
                                                                                                                                                                        ELIfe Final Report

Jahreis         Gerhard       Gerhard.Jahreis@uni-jena.de                University of jena         Institut of Nutrition     ++49-03641-        D-07743   Jena         Dornburger Str. 24      Germany
                                                                                                                              949610
Humpf           Hans-Ulrich   humpf@uni-muenster.de                      University of Muenster     Institut for Food         +49 251 83 33867   48149     Muenster     Corrensstr. 49          Germany
                                                                                                    Chemistry
Seedorf         Udo           seedorfu@uni-muenster.de                   University of Münster      Leibniz-Institute of      +49 251 8356181    48149     Münster      Domagkstr. 3            Germany
                                                                                                    Arteriosclerosis
                                                                                                    Research
Robenek         Horst         robenek@uni-muenster.de                    University of Münster      Leibniz-Institute of      +49 251 8356181    48149     Münster      Domagkstr. 3            Germany
                                                                                                    Arteriosclerosis
                                                                                                    Research
Klucken         Jochen        jochen.klucken@web.de                      University of              Department of             +49 941 9410       93051     Regensburg   Universitätsstr. 84     Germany
                                                                         Regensburg                 Neurology
Schmitz         Gerd          gerd.schmitz@klinik.uni-regensburg.de      University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Liebisch        Gerhard       gerhard.liebisch@klinik.uni-regensburg.de University of               Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                        Regensburg                  Chemistry                 6201                                      Strauss-Allee 11
Grandl          Margot        margot.grandl@klinik.uni-regensburg.de     University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Böttcher        Alfred        alfred.boettcher@klinik.uni-regensburg.de University of               Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                        Regensburg                  Chemistry                 6201                                      Strauss-Allee 11
Sigruener       Alex          alexander.sigruener@klinik.uni-            University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                              regensburg.de                              Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Leidl           Katharina     katharina.leidl@klinik.uni-regensburg.de   University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Schmitz-Madry   Anna          anna.schmitz-madry@klinik.uni-             University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                              regensburg.de                              Regensburg                 Chemistry                 6201                                      Strauss-Allee 11


Valis           Linda         linda1.valis@klinik.uni-regensburg.de      University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Kischel         Jette         jette.kischel@klinik.uni-regensburg.de     University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Mages           Josef         josef.mages@klinik.uni-regensburg.de       University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Wagenknecht     Daniela       daniela.wagenknecht@klinik.uni-            University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                              regensburg.de                              Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Wycislo         Matthias      matthias.wycislo@klinik.uni-               University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                              regensburg.de                              Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Lehneis         Stefan        stefan.lehneis@klinik.uni-regensburg.de    University of              Institute for Clinical    ++49-941-944-      93053     Regensburg   Franz-Josef-            Germany
                                                                         Regensburg                 Chemistry                 6201                                      Strauss-Allee 11
Naim            Hassan Y.     hassan.naim@tiho-hannover.de               University of Veterinary   Department of             ++49-511-953       D-30559   Hannover     Bünteweg 17             Germany
                                                                         Medicine Hannover          Physiological Chemistry   8780



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                                                                                                                                                                  ELIfe Final Report

Lekka            Marilena E.    mlekka@cc.uoi.gr               University of Ioannina       Laboratory of Lipid         +3026510-98367     451 10   Ioannina      Panepistemioupolis Greece
                                                                                            Analysis
Rühl             Ralph          rruehl@indi.biochem.dote.hu    Department of                Laboratory of Nutritional   +36 30 320 97 69   4012     Debrecen      Nagyerdei Krt. 98        Hungary
                                                               Biochemistry and             Bioactivation and
                                                               Molecular Biology,           Bioanalysis
                                                               University of Debrecen

Vigh             Laszlo         vigh@brc.hu                    Institute of Biochemistry,   Laboratory of Molecular     +36-62-432-048     H-6726   Szeged        Temesvari krt. 62.       Hungary
                                                               Biological Research          Stress Biology
                                                               Centre, HUng. Acad.
                                                               Sci.
Balogh           Gabor          Baloghg@BRC.HU                 Institute of Biochemistry,   Laboratory of Molecular     +36-62-432-048     H-6726   Szeged        Temesvari krt. 62.       Hungary
                                                               Biological Research          Stress Biology
                                                               Centre, HUng. Acad.
                                                               Sci.
L. Kiss          Anna           Kissa@ana2.sote.hu             Semmelweis University        Dept of Human               36 1 215           1094     Budapest      Tuzolto u. 58            Hungary
                                                                                            Morphology and              6924/3610
                                                                                            Developmental Biology
Szabo            Gabor          szabog@dote.hu                 University of Debrecen       Dept. Biophysics and        ++36-52-455866     4010     Debrecen      Egyetem ter 1.           Hungary
                                                                                            Cell Biology
Matyus           Laszlo         lmatyus@jaguar.dote.hu         University of Debrecen       Dept. Biophysics and        ++36-52-455866     4010     Debrecen      Egyetem ter 1.           Hungary
                                                                                            Cell Biology
Goda             Katalin        goda@jaguar.unideb.hu          University of Debrecen       Dept. Biophysics and        ++36-52-455866     4010     Debrecen      Egyetem ter 1.           Hungary
                                                                                            Cell Biology
Porath           Amiram         amiramp@post.tau.ac.il         Tel Aviv University          Sackler School of           972-3-6407842      69978    Tel Aviv      Levanon                  Israel
                                                                                            Medicine-Minerva
                                                                                            Center
Futerman         Tony           tony.futerman@weizmann.ac.il   Weizmann Institute           Dept of Biological          ++972-8-9342704    76100    Rehovot       1 Herzl Street           Israel
                                                                                            Chemistry
Chatgilialoglu   Chryssostomos chrys@isof.cnr.it               Consiglio Nazionale          ISOF/BioFreeRadicals        ++39-051-639-      40129    Bologna       Via Piero Gobetti        Italy
                                                               delle Ricerche                                           8309                                      101
MEGLI            Francesco M.   f.m.megli@biologia.uniba.it    Dept Biochemistry and        EPR Lipidomics              ++39 080 5443367 70126      BARI          V. E. Orabona, 4         Italy
                                                               Molecular Biology
Greco            Anita          agreco@iss.it                  Dept of Cell Biology and     Istituto Superiore di       ++39-06-           00161    ROME          viale Regina Elena, Italy
                                                               Neurosciences                Sanità                      49903087                                  299
Vaccaro          Anna Maria     annamaria.vaccaro@iss.it       Istituto Superiore di        Anna Maria Vaccaro          ++39 06 4990       00161    Roma          Viale Regina Elena       Italy
                                                               Sanita                       Unit of Physiopatology      2416                                      299
                                                                                            of genetic diseases
Luini            Alberto        dcbo@negrisud.it               Mario Negri Sud Institute Department of Cell             +39 0872570353     66030    Santa Maria   Via Nazionale 8/A        Italy
                                                                                         Biology and Oncology                                       Imbaro
Corda            Daniela        corda@negrisud.it              Mario Negri Sud Institute Department of Cell             +39 0872570353     66030    Santa Maria   Via Nazionale 8/A        Italy
                                                                                         Biology and Oncology                                       Imbaro
Masserini        Massimo        massimo.masserini@unimib.it    University Milano-           Biochemistry group          ++39-02-6448-      20052    Monza         Via Cadore, 48           Italy
                                                               Bicocca                                                  8203


                                                                                                                                                                                      73
                                                                                                                                                              ELIfe Final Report

Miserocchi   Giuseppe        massimo.masserini@unimib.it         University Milano-       Department of             ++39-02-6448-      20052      Monza       Via Cadore, 48           Italy
                                                                 Bicocca                  Experimental Medicine     8203
Pitto        Marina          marina.pitto@unimib.it              University Milano-       Department of             ++39-02-6448-      20052      Monza       Via Cadore, 48           Italy
                                                                 Bicocca                  Experimental Medicine     8203
Camici       Guido           camici@scibio.unifi.it              University of Florence   Dipartimento di Scienze   ++390554598328     50143      Firenze     Viale G.B.               Italy
                                                                                          Biochimiche                                                         Morgagni 50
ALBI         ELISABETTA      EALBI@UNIPG.IT                      University of Perugia    Department of Clinical    ++39-075-          06100      Perugia     Policlinico              Italy
                                                                                          and Experimental          5729085                                   Monteluce, via
                                                                                          Medicine,                                                           Brunamonti
                                                                                          Physiopathology,
                                                                                          University School of
                                                                                          Medicine

Aveldaño     Marta Isabel    avelda@criba.edu.ar                 University of Perugia    Department of Clinical    ++39-075-          06100      Perugia     Policlinico              Italy
                                                                                          and Experimental          5729085                                   Monteluce, via
                                                                                          Medicine,                                                           Brunamonti
                                                                                          Physiopathology,
                                                                                          University School of
                                                                                          Medicine

Corazzi      Lanfranco       corazzi@unipg.it                    University of Perugia    Department of Internal    0039 75 5857423    06122      Perugia     Via del Giochetto        Italy
                                                                                          Medicine
Goracci      Gianfrancesco   goracci@unipg.it                    University of Perugia    Department of Internal    ++39-075-585-      I-06122    Perugia     Via del Giochetto        Italy
                                                                                          Medicine, Section of      7420
                                                                                          Biochemistry
Corazzi      Lanfranco       corazzi@unipg.it                    University of Perugia    Department of Internal    ++39-075-585-      I-06122    Perugia     Via del Giochetto        Italy
                                                                                          Medicine, Section of      7420
                                                                                          Biochemistry
Funato       Koichi          kfunato@hiroshima-u.ac.jp           Hiroshima University     Funato Group              ++81-82-424-7925   739-8528   Higashi-    1-4-4,                   Japan
                                                                                                                                                  Hiroshima   Kagamiyama
Tojo         Hiromasa        htojo@mr-mbio.med.osaka-u.ac.jp     Osaka University         Department of             ++81-6-6879-3283   662-0018   Suita       2-2 Yamadaoka            Japan
                                                                 Graduate School of       Biochemistry and
                                                                 Medicine                 Molecular Biology
Asano        Chiyuki         asayuki@mr-mbio.med.osaka-u.ac.jp   Osaka University         Department of             ++81-6-6879-3283   662-0018   Suita       2-2 Yamadaoka            Japan
                                                                 Graduate School of       Biochemistry and
                                                                 Medicine                 Molecular Biology
Yasugi       Etsuko          e-yasugi@umin.net                   The University ofTokyo   Department of             81-3-5841-3651     113-0033   Tokyo       7-3-1, Hongo,            Japan
                                                                                          Metabolome, Graduate                                                Bunkyo-ku
                                                                                          School of Medicine
Kulik        Willem          w.kulik@amc.uva.nl                  Academic Medical         Laboratory Genetic        +31-20-5669111     NL-1100    Amsterdam   P.O. Box 22660           Netherlands
                                                                 Center                   Metabolic Diseases                           DD
Houtkooper   Riekelt         r.h.houtkooper@amc.uva.nl           Academic Medical         Laboratory Genetic        +31-20-5669111     NL-1100    Amsterdam   P.O. Box 22660           Netherlands
                                                                 Center                   Metabolic Diseases                           DD



                                                                                                                                                                                  74
                                                                                                                                                              ELIfe Final Report

van Lenthe       Henk              H.vanLenthe@amc.uva.nl          Academic Medical          Laboratory Genetic      +31-20-5669111    NL-1100   Amsterdam    P.O. Box 22660         Netherlands
                                                                   Center                    Metabolic Diseases                        DD
van der Hoeven   Rob               rob.hoeven-van-der@dsm.nl       analysis/spectrometry     DSM                     0031 152792278    2600 MA   Delft        p.o. box 1             Netherlands

Demacker         Pierre            P.Demacker@aig.umcn.nl          Dept Internal Medicine,   Lipid laboratory        -                 6500 HB   Nijmegen     Geert Grooteplein      Netherlands
                                                                   UMC Nijmegen                                                                               zuid 8
Schmidt          Thomas            schmidt@physics.leidenuniv.nl   Leiden Institute of       Physics of Life         +31 71 527 5982   2333 CA   Leiden       Niels Bohrweg 2        Netherlands
                                                                   Physics, Leiden           Processes
                                                                   University
Pool             Wim               pool@nioz.nl                    NIOZ                      NIOZ                    31 222 369582     1790 AB   Den Burg     P.O. Box 59            Netherlands
Kahya            Nicoletta         nicoletta.kahya@philips.com     Philips                   Philips Research,       +31-40-2743794    5656 AE   Eindhoven    High Tech Campus Netherlands
                                                                                             Molecular Diagnostics                                            4
Leeflang         Bas R.            b.r.leeflang@chem.uu.nl         Utrecht University        Bijvoet Center          ++31-30-2533498   NL-584    Utrecht      Padualaan 8            Netherlands
                                                                                                                                       CH
Egmond           Maarten           m.r.egmond@chem.uu.nl           Utrecht University        Bijvoet Center          ++31-30-2533498   NL-584    Utrecht      Padualaan 8            Netherlands
                                                                                                                                       CH
van Meer         Gerrit            g.vanmeer@chem.uu.nl            Utrecht University        Bijvoet Center          ++31-30-2533498   NL-584    Utrecht      Padualaan 8            Netherlands
                                                                                                                                       CH
Gómez-           Juan C.           jcgomez@um.es                   Utrecht University        Bijvoet Center          ++31-30-2533498   NL-584    Utrecht      Padualaan 8            Netherlands
Fernández                                                                                                                              CH
de Kroon         Toon              a.i.p.m.dekroon@chem.uu.nl      Utrecht University        Bijvoet Center          ++31-30-2533498   NL-584    Utrecht      Padualaan 8            Netherlands
                                                                                                                                       CH
de Cock          J.J.P.A. (Hans)   IB.deCock@bio.uu.nl             Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
van Meer         Gerrit            g.vanmeer@chem.uu.nl            Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Breukink         Eefjan            e.j.breukink@chem.uu.nl         Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Brouwers         Jos               j.brouwers@vet.uu.nl            Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Rottier          Peter             p.rottier@vet.uu.nl             Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Rodenburg        Kees W.           k.w.rodenburg@bio.uu.nl         Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Burger           Koert             K.N.J.Burger@bio.uu.nl          Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
van der Horst    Dick              D.J.vanderHorst@bio.uu.nl       Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Helms            Bernd             j.b.helms@vet.uu.nl             Utrecht University        Institute of            +31-30-2536616    3584 CH   Utrecht      Padualaan 8            Netherlands
                                                                                             Biomembranes
Kersten          Sander            sander.kersten@wur.nl           Wageningen University     Nutrition, metabolism   ++31-317-485787   6703 HD   Wageningen   Bomenweg 2             Netherlands
                                                                                             and genomics group
Skotland         Tore              tore.skotland@ge.com            GE Healthcare             Preclinical sciences    +47 2318 5666     0401      Oslo         Nycoveien 2            Norway




                                                                                                                                                                                75
                                                                                                                                                                 ELIfe Final Report

Sandvig           Kirsten     ksandvig@radium.uio.no             The Norwegian Radium      Inst. for Cancer            47 22934294        0310      Oslo         Montebello              Norway
(adm./leader of                                                  Hospital                  Research/Sandvigs
research group)                                                                            group:Intracellular
                                                                                           transport

Pulaski           Lukasz      lpulaski@cbm.pan.pl                Centre for Medical        Laboratory of               ++48-42-2723639    93-232    Lodz         Lodowa 106              Poland
                                                                 Biology PAS               Transcriptional
                                                                                           Regulation
Macri             Beatrice    beatrice_macri@yahoo.com           Faculty of Biology,       Department of Animal        00-40-21-318 15    050095    Bucharest    Splaiul                 Romania
                                                                 University of Bucharest   Physiology and              69                                        Independentei, 91-
                                                                                           Biophysics                                                            95
Svetina           Sasa        sasa.svetina@biofiz.mf.uni-lj.si   University of Ljubljana   Institute of Biophysics,    -386 1 5437600     1000      Ljubljana    Lipiceva 2              Slovenia
                                                                                           Faculty of Medicine
GAMARRO           FRANCISCO   gamarro@ipb.csic.es                CONSEJO SUPERIOR          INSTITUTO DE                0034958181667      18100     ARMILLA-     Parque                  Spain
                                                                 DE INVESTIGACIONES        PARASITOLOGIA Y                                          GRANADA      Tecnológico de
                                                                 CIENTÍFICAS               BIOMEDICINA LOPEZ-                                                    Ciencias de la
                                                                                           NEYRA, Working                                                        Salud. Avda. del
                                                                                           Group:                                                                Conocimiento s
                                                                                           BIOCHEMISTRY AND
                                                                                           MOLECULAR
                                                                                           PHARMACOLOGY. Dr.
                                                                                           FRANCISCO
                                                                                           GAMARRO
Fabrias           Gemma       gfdqob@cid.csic.es                 CSIC                      IQAC/Research Unit on       34-93-4006115      08034     Barcelona    Jordi Girona 18         Spain
                                                                                           BioActive Molecules
Goñi              Félix M.    felix.goni@ehu.es                  CSIC and Universidad      Unidad de Biofísica         ++34-94-601-2625   48940     Leioa        Sarriena s              Spain
                                                                 del País Vasco
Mañes             Santos      smanes@cnb.uam.es                  Spanish Research          National Center of          ++34-91-5854840    28049     Madrid       Darwin, 3. Campus       Spain
                                                                 Council                   Biotechnology                                                         Cantoblanco
                                                                                                                                                                 University
Merida            Isabel      imerida@cnb.uam.es                 Spanish Research          National Center of          ++34-91-5854840    28049     Madrid       Darwin, 3. Campus       Spain
                                                                 Council                   Biotechnology                                                         Cantoblanco
                                                                                                                                                                 University
Balsinde          Jesús       jbalsinde@ibgm.uva.es              Spanish Research          Institute of Molecular      +34-983-423-062    E-47003   Valladolid   Calle Sanz y Fores      Spain
                                                                 Council & University of   Biology and Genetics                                                  s
                                                                 Valladolid School of
                                                                 Medicine

Perez-Gil         Jesus       perejil@bbm1.ucm.es                Universidad               BIOMIL (BIOphysics of       ++34-91-3944994    28040     Madrid       Fac. Biologia,          Spain
                                                                 Complutense               Membranes and                                                         Dept. Bioquimica
                                                                                           Lipid/Protein Interfaces)
Perillo           María       mperillo                           Universidad               BIOMIL (BIOphysics of       ++34-91-3944994    28040     Madrid       Fac. Biologia,          Spain
                                                                 Complutense               Membranes and                                                         Dept. Bioquimica
                                                                                           Lipid/Protein Interfaces)




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                                                                                                                                                                    ELIfe Final Report

Gómez-        Juan C.        jcgomez@um.es                          University of Murcia       Research Group in           ++34-968364766     E-30100   Murcia      Facultad de              Spain
Fernández                                                                                      Biomembranes,                                                        Veterinaria,
                                                                                               Department of                                                        Campus de
                                                                                               Biochemistry and                                                     Espinardo
                                                                                               Molecular Biology

Ochoa         Begoña         begona.ochoa@ehu.es                    University of the Basque   Dept. Physiology,           ++34946012846      48940     Bilbao      Sarriena                 Spain
                                                                    Country                    Medical School
RUIZ-LARREA   M. BEGOÑA      mbego.ruizlarrea@ehu.es                University of the Basque   Dept. Physiology,           ++34946012846      48940     Bilbao      Sarriena                 Spain
                                                                    Country                    Medical School
Ruiz-Sanz     Jose Ignacio   joseignacio.ruizs@ehu.es               University of the Basque   Dept. Physiology,           ++34946012846      48940     Bilbao      Sarriena                 Spain
                                                                    Country                    Medical School
Martínez      María José     mariajose.martinez@ehu.es              University of the Basque   Dept. Physiology,           ++34946012846      48940     Bilbao      Sarriena                 Spain
                                                                    Country                    Medical School
Jacobsson     Anders         anders.jacobsson@wgi.su.se             Stockholm University       The Wenner-Gren             ++46-8-164127      SE10691   Stockholm   Arrhenius lab. F3        Sweden
                                                                                               Institute
Nedergaard    Jan            jan@metabol.su.se                      Stockholm University       The Wenner-Gren             ++46-8-164127      SE10691   Stockholm   Arrhenius lab. F3        Sweden
                                                                                               Institute
Parmryd       Ingela         ingela.parmryd@cellbio.su.se           Stockholm University       The Wenner-Gren             ++46-8-164127      SE10691   Stockholm   Arrhenius lab. F3        Sweden
                                                                                               Institute
Carlsson      Anders         anders.carlsson@vv.slu.se              Swedish University of      Department of Crop          please fill in     23053     Alnarp      Box 44                   Sweden
                                                                    Agricultural Sciences      Science
Lindblom      Göran          goran.lindblom@chem.umu.se             Umeå University            Department of               +46907865228       SE-       Umeå        Linaeus väg 10           Sweden
                                                                                               Biophysical Chemistry                          90187
Geyer         Roland         roland.geyer@eur.appliedbiosystems.com Applera Europe B.V.        LC-MS Support               +41 41 799 7742    6343      Rotkreuz    Grundstrasse 10          Switzerland

Hopfgartner   Gérard         Gerard.Hopfgartner@pharm.unige.ch      School of                  Life Sciences Mass          +41-22-3796344     1211      Geneva      Bd Yvoy 20               Switzerland
                                                                    Pharmaceutical             Spectrometry
                                                                    Sciences - University of
                                                                    Geneva
Varesio       Emmanuel       Emmanuel.Varesio@pharm.unige.ch        School of                  Life Sciences Mass          +41-22-3796344     1211      Geneva      Bd Yvoy 20               Switzerland
                                                                    Pharmaceutical             Spectrometry
                                                                    Sciences - University of
                                                                    Geneva
De Libero     Gennaro        gennaro.delibero@unibas.ch             University Hospital,       University of Basel,        +41 61 2652365     4031      Basel       Hebelstrasse, 20         Switzerland
                                                                    Department of Research     Experimental
                                                                                               Immunology
Conzelmann    Andreas        andreas.conzelmann@unifr.ch            University of Fribourg     Division of Biochemistry    0041 26 300 8630   CH-1700   Fribourg    5, chemin du             Switzerland
                                                                                                                                                                    musée
Schneiter     Roger          roger.schneiter@unifr.ch               University of Fribourg     Division of Biochemistry    0041 26 300 8630   CH-1700   Fribourg    5, chemin du             Switzerland
                                                                                                                                                                    musée
Yu            John           johnyu@gate.sinica.edu.tw              Academia Sinica            Institute of Cellular and   ++886-2-           11529     Taipei      Nankang                  Taiwan
                                                                                               Organismic Biology,         27899531
                                                                                               Section of Stem Cells



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                                                                                                                                                                ELIfe Final Report

CHUANG          Nin-Nin     zonnc@sinica.edu.tw                  Academia Sinica           Institute of Cellular and   ++886-2-           11529     Taipei      Nankang                Taiwan
                                                                                           Organismic Biology,         27899531
                                                                                           Section of Stem Cells
Wu              Wen-guey    wgwu@life.nthu.edu.tw                National Tsing Hua        Institute of                886-3-5742752      30043     Hinchu      Kung-Fu Rd             Taiwan
                                                                 University                Bioinformatics and
                                                                                           Structural Biology
O Donnell       Valerie     o-donnellvb@cardiff.ac.uk            Cardiff University        Dept. of Medical            0044 29 2074       CF14      Cardiff     Heath Park             United
                                                                                           Biochemistry &              8447               4XN                                          Kingdom
                                                                                           Immunology, School of
                                                                                           Medicine

Wase            Nishikant   cpp06nvw@shef.ac.uk                  Chemical and Process      Chemical Engineering        +44-07809717355    S3 7RD    Sheffield   40 LeavyGreave         United
                                                                 Engineering University of Life Science Interface                                               Road                   Kingdom
                                                                 Sheffield
Schweizer       Michael     M.Schweizer@hw.ac.uk                 Heriot-Watt University    School of Life Sciences     ++44 131 451       EH14    Edinburgh     Roccarton              United
                                                                                                                       3186               4AS                                          Kingdom
Templer         Richard     r.templer@imperial.ac.uk             Imperial College London   The Chemical Biology        +44 (0)20 7594     SW7 2AZ London        Exhibition Road        United
                                                                 in association with the   Centre in the               5787                                                            Kingdom
                                                                 Institute of Cancer       Department of
                                                                 Research and the          Chemistry
                                                                 London Research
                                                                 Institute of CRUK

Mullard         Asher       a.mullard@nature.com                 NPG                       Nature Reviews              +44(0)2078433641 N1 9XW      London      4 Crinan St            United
                                                                                           Molecular Cell Biology                                                                      Kingdom
Butters         Terry       terry.butters@bioch.ox.ac.uk         Oxford University         Glycobiology Institute      +44 1865 275725    OX1 3QU Oxford        South Parks Rd         United
                                                                                                                                                                                       Kingdom
van der Spoel   Aarnoud     aarnoud.vanderspoel@bioch.ox.ac.uk   Oxford University         Glycobiology Institute      +44 1865 275725    OX1 3QU Oxford        South Parks Rd         United
                                                                                                                                                                                       Kingdom
Salisbury       Malcolm     Malcolm.Salisbury@shell.com          Shell Global Solutions    Shell Global Solutions      +44-151-373-5730   CH13SH    Chester     P.O. Box 1             United
                                                                 (UK)                      (UK)                                                                                        Kingdom
Wierzbicki      Anthony     Anthony.Wierzbicki@kcl.ac.uk         St Thomas Hospital        Refsum disease group        ++442071881256     SE1 7EH   London      Lambeth Palace         United
                                                                                                                                                                Road                   Kingdom
Griffiths       William     william.griffiths@ulsop.ac.uk        The School of Pharmacy    Mass Spectrometry           ++44-20-           WC1N      London      29/39 Brunswick        United
                                                                                           Facility                    77535876           1AX                   Square                 Kingdom
Karu            Kersti      kersti.karu@pharmacy.ac.uk           The School of Pharmacy    Mass Spectrometry           ++44-20-           WC1N      London      29/39 Brunswick        United
                                                                                           Facility                    77535876           1AX                   Square                 Kingdom
Scigelova       Michaela    michaela.scigelova@thermo.com        Thermo Electron           European application        +44 1442233555     HP2 7GE   Hemel       1 Boundary Park        United
                                                                                           laboratory                                               Hempstead                          Kingdom
SMITH           TERRY       T.K.Smith@dundee.ac.uk               UNIV OF DUNDEE            WELLCOME TRUST              ++44-(0)1382-      DD1 5EH   DUNDEE      HAWKHILL               United
                                                                                           BIOCENTRE, SCHOOL           388688                                                          Kingdom
                                                                                           OF LIFE SCIENCES,
                                                                                           UNIV OF DUNDEE




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                                                                                                                                                            ELIfe Final Report

Talmud        Philippa     p.talmud@ucl.ac.uk                University College        Cardiovascular           +44 207 679 6968   WC1E      London         5 University St         United
                                                             London                    Genetics,                                   6JF                                              Kingdom
Thies         Frank        f.thies@abdn.ac.uk                University of Aberdeen    Aberdeen lipidomix       44 (0)1224 553020 AB25       Aberdeen       Polwarth building,      United
                                                                                       group                                      2ZD                       Foresterhill            Kingdom
Lloyd         Matthew      M.D.Lloyd@bath.ac.uk              University of Bath       Department of             ++-44-1225-        BA2 7AY   Bath           Claverton Down          United
                                                                                      Pharmacy &                3867686                                                             Kingdom
                                                                                      Pharmacology
Wakelam       Michael      m.j.o.wakelam@bham.ac.uk          University of Birmingham Institute for Cancer      44-(0)121-414-     B15 2TT   Birmingham     Vincent Drive           United
                                                                                      Studies                   3293                                                                Kingdom
Nicolaou      Anna         a.nicolaou@bradford.ac.uk         University of Bradford    School of Pharmacy       ++44-1274-         BD7 1DP   Bradford       Richmond Road           United
                                                                                                                224717                                                              Kingdom
MASOODI       MOJGAN       m.masoodi@bradford.ac.uk          University of Bradford    School of Pharmacy       ++44-1274-         BD7 1DP   Bradford       Richmond Road           United
                                                                                                                224717                                                              Kingdom
Massey        Karen        k.massey@bradford.ac.uk           University of Bradford    School of Pharmacy       ++44-1274-         BD7 1DP   Bradford       Richmond Road           United
                                                                                                                224717                                                              Kingdom
Mir           Adnan        A.A.Mir@bradford.ac.uk            University of Bradford    School of Pharmacy       ++44-1274-         BD7 1DP   Bradford       Richmond Road           United
                                                                                                                224717                                                              Kingdom
Anyakoha      Ngozi        n.g.anyakoha@bradford.ac.uk       University of Bradford    School of Pharmacy       ++44-1274-         BD7 1DP   Bradford       Richmond Road           United
                                                                                                                224717                                                              Kingdom
van Veen      Hendrik W.   hwv20@cam.ac.uk                   University of Cambridge   Department of            44-1223-334032     CB2 1PD   Cambridge      Tennis Court Road       United
                                                                                       Pharmacology                                                                                 Kingdom
Sansom        Mark         mark.sansom@bioch.ox.ac.uk        University of Oxford      Dept. of Biochemistry    +44 1865 275371    OX1 3QU Oxford           South Parks Road        United
                                                                                                                                                                                    Kingdom
Postle        Tony         adp@soton.ac.uk                   University of             School of Chemistry      ++44 (0)2380       SO17      Southampton University Road            United
                                                             Southampton                                        796161             1BJ                                              Kingdom
Scaife        Jes          jes.scaife@writtle.ac.uk          Writtle College           Centre for Equine and    44 1245 424200     CM1 3RR Chelmsford       Lordship Road           United
                                                                                       Animal Science                                                                               Kingdom
Chikunya      Sife         sifelani.chikunya@writtle.ac.uk   Writtle College           Centre for Equine and    44 1245 424200     CM1 3RR Chelmsford       Lordship Road           United
                                                                                       Animal Science                                                                               Kingdom
Moye-Rowley   Scott        scott-moye-rowley@uiowa.edu       Dept. of Molecular        University of Iowa       1-319-335-7874     52246     Iowa City      6-530 Bowen             United
                                                             Physiology and                                                                                 Science Building        States
                                                             Biophysics
BARD          Martin       mbard@iupui.edu                   Indiana Umiversity -      Indiana Umiversity -     317-274-0593       46202     Indianapolis   723 W. Michigan         United
                                                             Purdue University         Purdue University                                                    St.                     States
                                                             Indianapolis              Indianapolis
Yu            Robert       ryu@mcg.edu                       Medical College of        Institute of Molecular   01-706-721-0699    GA        Augusta        1120 15th Street        United
                                                             Georgia                   Medicine and Genetics                       30912                                            States
Reid          Scott        reidsk@musc.edu                   Medical University of     COBRE in Lipidomics &    843-792-4323       29425     Charleston     PO Box 250509           United
                                                             South Carolina            Pathobiology                                                                                 States
Hama          Hiroko       hama@musc.edu                     Medical University of     COBRE in Lipidomics &    843-792-4323       29425     Charleston     PO Box 250509           United
                                                             South Carolina            Pathobiology                                                                                 States




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Frohman   Michael   michael@pharm.stonybrook.edu   Stony Brook University      Center for             please fill in       11794    Stony Brook   438 CMM                United
                                                                               Developmental Genetics                                                                    States
Polt      Robin     polt@u.arizona.edu             The University of           The Polt Group           ++01-520-621-      AZ 85721 Tucson        Department of          United
                                                   Arizona                                              6322                                      Chemistry              States
Cotter    Dawn      dcotter@sdsc.edu               University of California    San Diego                858-822-3619       92093    La Jolla      9500 Gilman Drive,     United
                                                   San Diego                   Supercomputer Center /                                             Mail Code 0412         States
                                                                               LIPID MAPS
                                                                               Bioinformatics Core

Fahy      Eoin      fahye@sdsc.edu                 University of California    San Diego                858-822-3619       92093    La Jolla      9500 Gilman Drive, United
                                                   San Diego                   Supercomputer Center /                                             Mail Code 0412     States
                                                                               LIPID MAPS
                                                                               Bioinformatics Core

Maer      Andreia   amaer@sdsc.edu                 University of California    San Diego                858-822-3619       92093    La Jolla      9500 Gilman Drive, United
                                                   San Diego                   Supercomputer Center /                                             Mail Code 0412     States
                                                                               LIPID MAPS
                                                                               Bioinformatics Core

Sud       Manish    msud@sdsc.edu                  University of California    San Diego                858-822-3619       92093    La Jolla      9500 Gilman Drive, United
                                                   San Diego                   Supercomputer Center /                                             Mail Code 0412     States
                                                                               LIPID MAPS
                                                                               Bioinformatics Core

Ordovas   Jose      jose.ordovas@tufts.edu         USDA-Human Nutrition        Nutrition and Genomics   ++1-617-556-3102   02111    Boston        711 Washington St      United
                                                   Research Center on                                                                                                    States
                                                   Aging at Tufts University
McPhail   Linda     lmcphail@wfubmc.edu            Wake Forest University      Department of            please fill in     27157    Winston-      Medicial Center        United
                                                   School of Medicine          Biochemistry                                         Salem         Boulevard              States




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                                  Qui ckT ime™ and a
                        T IFF (Uncompressed) decompressor
                            are needed to see thi s pi cture.




                                   LSSG-CT-2004-013032
                                             ELIfe
                    European Lipidomics Initiative: Shaping the Life Sciences

Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6




          TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

                 Plan for Using and Disseminating Knowledge
                              (extended version)



                                                                Final Report
                                                                 2005-2007




Period covered: from 1/1/05 to 1/7/07                                            Date of preparation: 1/1/08
Start date of project: 1/1/05                                                    Duration: 2.5 years
Project coordinator name:                                         Prof. Gerrit van Meer
Project coordinator organisation name:                            Utrecht University




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Section 1 - Exploitable knowledge and its use
The objectives of the Specific Support Action were:
Objective 1: To network the field of Metabolomics and to seek alliances with relevant stakeholders
Objective 2: To link the field of Metabolomics to the genomics and proteomics initiatives.
Objective 3: To define a strategy for Metabolomics research, using Lipidomics as an example.
Objective 4: To initiate an Expertise Platform on Lipidomics
Objective 5: To hold both science-related as well as policy meetings

One product of the SSA is the participation of roughly one thousand scientists, clinicians and
industrial stakeholders in the ELIfe workshops and meetings, and the resulting networking between
them. A second product is the interactive Lipidomics Expertise Platform on the ELIfe website. A
third product is formed by the papers published in the international literature. As such there are no
exploitable results.

Section 2 – Dissemination of knowledge
                                                                             Size of     Partner
 Dates          Type                Type of Audience           Countries     Audience    involved

 2005           Flyer               General                    World                     1

 20-21 May
 05             Workshop            Lipid technology           Global                    2,3
                                    Young Basic
 3-6 Sept 05    Conference          Scientists                 Europe           1,500    3
 25-28 Sept
 05             Conference          Industry                   Europe              200   2,12

                                                               Europe
 3-7 June 06    Workshop 3.1        Cell Biologists            +US                 155   1,3,7
 5-6 Sept 06    Workshop 3.2        Clinicians/lipidologists   Europe               80   6,11,14
                Conference
 5-6 Sept 06    1.3.2               Lipidologists              Europe              150   2,3,11,14
                Conference
 1-4 Oct 06     1.3.1               Industry                   Europe               55   2,9
 21-25 Oct                                                                               1 through
 06             Conference 1.5      General                    World               254   14
 23-24 Oct
 06             Meeting 3.3         General; industry          World                60   2,12

                                                                                         1 through
 3-Oct-05       Advertorial         European Parliament        Europe                    14
 2005           Interview           General scientist          Europe                    1,12
 2006           Interview           General                    World                     12

 2005           Paper   1           General                                              1,12
                Paper   2           Basic scientists                                     12
                Paper   3           General                                              12
                Paper   4           General                                              12
                Paper   5           Basic scientists                                     1

 2006           Paper   1           Lipidol/industry                                     5
                Paper   2           Lipidol/industry                                     1
                Paper   3           General                                              2
                Paper   4           Lipidol/industry                                     12
                Paper   5           Lipidol/industry                                     12
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                Paper   6           Lipidol/industry                                   1
                Paper   7           General                                            1,2,7
                Paper   8           Lipidol/clinicians                                 1
                Paper   9           Lipidol/clinicians                                 1-3,7,12
                Paper   10          Basic scientists                                   1,3

 2007           Lipidomics Grant                                                       1,2,8,10,12

 2007           Survey              General                                            2

Conferences.
The conferences organized under the present SSA were:

1.5 ELIfe/Special FEBS meeting
- The general ELIfe meeting entitled 'New concepts in lipidology: from lipidomics to disease'
(Noordwijkerhout, NL, 21-25 October 2006) was cosponsored as a FEBS special meeting (AP6a)
and was attended by over 250 participants, (http://www.febslipid2006.chem.uu.nl/). The speaker
selection was performed by the FEBS and ELIFE organizing committee (Ben de Kruijff, Pete
Downes, Bernd Helms, Kai Simons, Gerrit van Meer (chair) and Felix Wieland), and the other
ELIfe members by a written procedure. The meeting hosted a short joint symposium between the
Nordrhein-Westfälische Akademie der Wissenschaften and the Dutch Royal Academy of Arts and
Sciences contributed by Konrad Sandhoff, Bonn.
- The meeting hosted the industry meeting (see under T3.3).
- Bernd Helms and Gerrit van Meer edited a special ELIfe issue of FEBS Letters under the title
"Lipidome and Disease" which was distributed at the meeting.

2.2 Mass spec workshop (Dresden, 20-21 May 2005)
- This was a successful workshop on mostly technical aspects of mass spectrometry with a final
discussion focusing on what is required to apply this technology to the broader field of lipidomics,
especially the cell biological and medical applications. The meeting was attended by mostly mass
spectroscopists. The American LIPID MAPS consortium was well-represented, and also the
Japanese LipidBank consortium was represented.
- In a meeting of the Executive Committee (van Meer, Schmitz, Spener, Simons) with the
International Lipids Classification and Nomenclature Committee (Ed Dennis, Chris Raetz, Bob
Murphy, Fritz Spener, Gerrit van Meer, Yousuke Seyama and Takao Shimizu), actually attended by
Ed Dennis, Masahiro Nishijima, Gerd Schmitz, Fritz Spener, Kai Simons, Ryo Taguchi, and Gerrit
van Meer, all agreed to use one common database number for any lipid. The number would be
preceeded by the name of the database in which the lipid was entered. LIPID MAPS will propose a
procedure for how to handle this in practice.
- The workshop was also visited by the EC project officer, Christina Kyriakopoulou, who confirmed
the importance of bringing the lipidomics initiative to fruition.

3.1 Workshop Dynamic Lipid Organization in Cells
The EMBO/ELIfe workshop "Dynamic Lipid Organization in Cells" was held in Bilbao, 3-7 June
2006 by Kai Simons, Gisou van der Goot, Felix Goñi and Gerrit van Meer.
       Co-sponsoring by EMBO was requested and awarded. The workshop was advertised all
over Europe. The response was great and the workshop was a big success. The workshop was
attended by 120 participants and had 35 speakers from all over the world, but mostly from Europe.
There were plenary discussions, poster sessions and round tables. The poster sessions worked
extremely well with lots of discussions at each poster over the whole meeting.
       Conclusions from the workshop were that lipidomics is now ripe for funding from the next
EC framework programme. The technology in mass spectrometry and also many different
biophysical techniques, such as fluorescence correlation spectroscopy and single molecule
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microscopy are paving the way for a exploration of the functions of lipids in normal cell physiology
and in disease. Europe has a very strong research base in this area, which needs to be defended.
        The outcome of the workshop was used as a preparation for the definition of possible
spearpoints in the next EC framework programme. Questions that could be addressed are protein
lipid interactions in structure biology, a role of single lipids cholesterol rafts in cellular function and
disease, the role of lipid droplets in normal physiology and obesity (the latter topic has been worked
out in the LipidomicNet proposal which has been selected for funding under FP7).

3.2 Workshop Lipidomics and Health
As decided unanimously in the Kick-off meeting, the ELIfe workshop was held as a satellite to the
ICBL 2006 meeting in Pécs, 5-10 September 2006 (http://www.icbl2006.hu/). The ELIfe workshop
was organized by Balázs Sarkadi and Andras Varadi (local organizers), Gerd Utermann, and Pam
Fredman. It was organized in excellent collaboration with the ICBL organizer Laszlo Vigh, and
with the ICBL chair (and ELIfe member) Michel Lagarde who also managed to involve the
International Society for the Study of Fatty Acids and Lipids (ISSFAL).

3.3 Industry meeting
Within the ELIfe/FEBS Special Meeting “New Concepts in Lipidology: from Lipidomics to
Disease” two half-day sessions were devoted to “Industrial and Technological Aspects of
Lipidomics”, chaired by Dr. Ron Potman (Unilever, chair of ELIfe advisory board, president of
Euro Fed Lipid) and Dr. Fritz Spener (ELIfe board member, University of Graz). Speakers were
either from industry or applied-research oriented academic institutions. A third short session
“Lipidomics and Metabolomics” was chaired by Drs. Peter Downes and Fran Platt. Almost all
speakers were present at the Round Table Discussion chaired by Dr. Spener at the end of the second
half-day industrial session with more than 60 participants.


The public conferences to which ELIfe contributed were:

1.3 Symposia
- The speakers were asked to connect from their different background metabolomics/lipidomics
with genomics/proteomics, and where possible relate their talks to medically relevant issues and
industrial applications. The idea was to enthuse broad audiences of scientists from the life sciences
(ELSO), industry (ISF) and biomedicine/biochemistry (ICBL) for the new developments in
metabolomics/lipidomics. The ELIfe members had discussions on the continuation of the
lipidomics initiative in Frankfurt, August 2006 and February 2007.

1.3.1a The ELSO 2005 conference
The ELSO 2005 conference, 3-7 September in Dresden (D), was attended by 1,200 scientists,
amongst whom 40% PhD students. The program consisted of 6 plenary sessions, 21 minisymposia
(in 3 sessions of 7 parallel symposia), 3 poster sessions and 7 sub-group meetings. ELIfe
contributed Minisymposium 1: "Lipidomics", chaired by Pam Fredman and Gerrit van Meer. After
an introduction of the EC-funded European Lipidomics Initiative and scientific ELIfe presentations
by the chairs, the invited lecturer Kim Ekroos (Astra-Zeneca, Mölndal, Sweden) presented a lecture
entitled "Unravelling phospholipidomes and sphingolipidomes by lipid profiling". This was
followed by three presentations selected from the poster abstracts. The minisymposium was
attended by over 100 participants.
         The purpose of having this session was to expose a broad audience of life scientists
including many young scientists to lipidomics from three different angles, basic (van Meer),
medical (Fredman) and industry (Ekroos) and to try and make them enthusiastic for this upcoming
field. This field can only develop in its whole breadth if young scientists from different disciplines
join in. The lecturers were available for discussion at this networking meeting for one more day.

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1.3.1b The 4th Euro Fed Lipid congress
- Unexpectedly, ELSO did not organize its meeting in 2006. Instead, ELIfe contributed a session to
the 4th Euro Fed Lipid Congress – Fats, Oils and Lipids for a Healthier Future, Madrid, Spain
(October 1-4, 2006). One 4 hour session was contributed on Lipid Mediators and Lipidomics,
chaired by Fritz Spener and Michel Lagarde (http://www.eurofedlipid.org/meetings/madrid/). This
was a meeting with an industrial character.

1.3.2a International Society for Fat Research
At the 26th World Congress and Exhibition of the International Society for Fat Research in Prague,
Czech Republic, 25-28 September 2005, hosted by the Czech Chemical Society and Euro Fed
Lipid: 26th ISF World Congress "Modern aspects of fats and oils--A fascinating source of
knowledge", the session on "Lipid Bioscience and Genomics" was sponsored by ELIfe. It was
chaired by Fritz Spener and J. Kas:
- Ben van Ommen, Project coordinator of NUGO (www.nugo.org/metabolomics), TNO Nutrition
and Food Research, Zeist, NL: "Food and health approached by lipidomics and nutrigenomics".
- David A. Bernlohr, University of Minnesota, Minneapolis, MN, USA: "Proteomics of lipid
oxidation in diabetes and obesity".
The International Society for Fat Research and the European Federation for the Science and
Technology of Lipids, like the American Oil Chemists Society have a strong industrial character,
meaning that their activities are industry oriented and are rarely attended by life scientists and
clinical scientists.

1.3.2b International Conference on the Bioscience of Lipids
- ELIfe contributed to the 47th International Conference on the Bioscience of Lipids (ICBL - ELIfe
- ILPS) joint meeting, 5-10 September, 2006 in Pécs, Hungary (http://www.icbl2006.hu). One full
day was dedicated to two joint ICBL-ELIfe sessions on Lipidomics and Membrane microdomains,
co-chaired by Gerd Schmitz, Gabor Balogh, Gerrit van Meer and Janos Szöllõsi.
        In the joint ICBL–ELIfe session on Lipidomics, Markus Wenk of National University of
Singapore discussed lipidomics of host-pathogen interactions. Edward A. Dennis, University of
California at San Diego, La Jolla CA, USA, told about the progress in LIPID MAPS and eicosanoid
lipidomics. Gerd Schmitz of University of Regensburg, Germany, focussed on the effect of
differential raft regulation in human macrophages upon Ox-LDL and E-LDL loading.
        In the session Membrane Microdomains, Janos Szollosi of the Hungarian Academy of
Sciences, Debrecen, Hungary, told that membrane microdomains are distinct molecular association
clusters with function properties. Gerhard Schütz of Johannes Keppler University Linz, Austria, had
a look onto the nanoscopic organization of the plasma membrane using single molecule
microscopy. Unfortunately, Akihiro Kusumi of Nagoya University could not attend the meeting.
His plenary talk was replaced by Toshihide Kobayashi, Riken, Japan. He introduced the
combination of different novel cholesterol probes with the capability to reveal the cholesterol
gradient in cell membranes. Finally, Gerrit van Meer of Utrecht University, The Netherlands,
discussed dynamic organization and unexpected function of typical raft lipids.

Papers
2005:
1.a Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy, C.R. Raetz,
D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer, M.S. Vannieuwenhze,
S.H. White, J. Witztum, and E.A. Dennis (2005) A comprehensive classification system for lipids.
J. Lipid Res. 46, 839-861.
1.b idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
2. Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M., Bogdanov, A.,
Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle, T., Jarv, J., Karyakin, A.,

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Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I., Spener, F., Voyer, N., and Wild, J.
(2005) Postgenomic chemistry (IUPAC Technical Report). Pure Appl. Chem. 77, 1641–1654.
3. Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci. Technol.
107, 1-2.
4. Spener F. (2005) Lipidomics and consequences: a new classification system for lipids. Eur. J.
Lipid Sci. Technol. 107, 277-278.
5. van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.

2006:
1. Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–708.
2. Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci. Technol.
108, 895–897.
3. Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study structural and
functional defects of ABC-transporters in cellular lipid trafficking. FEBS Lett. 580, 5597-5610.
4. Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies – from
innocent bystanders to prime targets of lipid research for combating human diseases. Eur. J. Lipid
Sci. Technol. 108, 541-543.
5. Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J. Lipid Sci.
Technol. 108, 625-627.
6. van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J. Lipid Sci.
Technol. 108, 799–801.
7. van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The European
lipidomics initiative: enabling technologies. Methods Enzymol. 432, 213-232.

8. Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special Issue.
FEBS Lett. 580, 5429-5610.
A special ELIfe issue of FEBS Letters "Lipidome and Disease", edited by Gerrit van Meer and
Bernd Helms, drawing attention to the general meeting and the ELIfe activities, distributed at the
ELife/FEBS meeting.

9. van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U., Bougnoux,
P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., Julià-Sapé, M., Larijani, B.,
Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B., Record, M., Schmitz, G., Simons, K.,
Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R., Wakelam, M.J.O., and Wanders, R.J.A. (2008)
Structural Medicine II: the Importance of Lipidomics for Health and Disease, European Science
Foundation Policy Briefing, in press.
The policy briefing specifically acknowledges EC specific support action LSSG-CT-2004-013032.

10. van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G., Warren,
G. (2008) Membrane Architecture and Dynamics (EuroMembrane), EuroCore theme proposal. Call
to be launched in spring 2008.

Interviews:
L. Winckler (2005) Lipidomics. Laborjournal 12, 20-23.
A paper aimed at the general biomedical community and based on interviews with ELIfe
participants and their team members.

C.D. Hillyer (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206
With initiatives and consortiums around the world garnering significant grant money for lipidomics
research, the field as a whole is poised to make an important contribution to the ever-increasing
pool of knowledge about the role of lipids in human disease and metabolism. Interview with Fritz
Spener and representatives of the US and Japanese Lipidomics Initiatives.

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Advertorial:
Parliament Magazine, issue 210 of October 3, 2005, entitled "Fat is Bad?", stressing the need for
financial support under FP7 for the area of metabolomics, and of lipidomics in particular.

Survey:
The data in the Lipidomics Expertise Platform database have been analyzed and the survey will be
published on the website (see Annex 2 of the second periodic report).

Other activities:
- 2,500 scientists with an interest in lipids have been approached by e-mail in March and August
2006 with an invitation to attend the ELIfe general meeting.

- Scientists outside the ELIfe consortium have been actively invited to take part in the ELIfe
discussions on the development of lipidomics in a broader context. The "White Paper" on "Enabling
Technologies for Studying the Genome, Proteome and Cytome of the Lipidome" has been made
publicly available in an interactive format on the LEP website (http://www.lipidomics-
expertise.de/). LEP-Wiki serves as the basis for defining areas within the lipidomics field where
research is expected to have the largest impact for society, especially in the form of health benefits.

- One issue is the prolongation of the European Lipidomics Initiative in the form of actual
collaborations. A further result of the ELIfe SSA was the formation of a European consortium to
submit in the first call of the FP7 a grant application for a large collaborative project (21 partners
including 4 SMEs, coordinated by G. Schmitz, Regensburg), entitled 'Lipid droplets as dynamic
organelles of fat deposition and release: Translational research towards human disease'
(LipidomicNet).

- Finally, the Eurocores theme proposal Euromembrane that was discussed at the first annual
meeting has been adopted by ESF.

Section 3 - Publishable results

All papers have been published.




                                                                                                         87
ELIfe Final Report




               88
                                                                                                         ELIfe Final Report


                                  Qui ckT ime™ and a
                        T IFF (Uncompressed) decompressor
                            are needed to see thi s pi cture.




                                   LSSG-CT-2004-013032
                                             ELIfe
                    European Lipidomics Initiative: Shaping the Life Sciences

Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6




          TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

                 Plan for Using and Disseminating Knowledge
                             (publishable version)



                                                                Final Report
                                                                 2005-2007




Period covered: from 1/1/05 to 1/7/07                                            Date of preparation: 1/1/08
Start date of project: 1/1/05                                                    Duration: 2.5 years
Project coordinator name:                                         Prof. Gerrit van Meer
Project coordinator organisation name:                            Utrecht University




                                                                                                                        89
                                                                                      ELIfe Final Report




                                                                             Size of      Partner
 Dates          Type                 Type of Audience         Countries      Audience     involved

 2005           Paper   1            General                  World                       1,12
                Paper   2            Basic scientists                                     12
                Paper   3            General                                              12
                Paper   4            Basic scientists                                     1
 2006           Paper   5            General                                              2
 2007           Paper   6            General                                              1,2,7
                Paper   7            General                                              1-14
 2005           Paper   8            General                                              12
 2006           Paper   9            Lipidol/industry                                     5
                Paper   10           Lipidol/industry                                     1
                Paper   11           Lipidol/industry                                     12
                Paper   12           Lipidol/industry                                     12
                Paper   13           Lipidol/industry                                     1
 2008           Paper   14           Lipidol/clinicians                                   1-3,7,12
                Paper   15           Basic Scientists                                     1,3
 2006           Paper   16           Lipidol/clinicians                                   1

 2005           Interview 17         General scientist        Europe                      1,12
 2006           Interview 18         General                  World                       12

 2005           Flyer                General                  Europe                      1
 2005           Advertorial          European Parliament      Europe                      1-14

 2007           Survey               General                                              2

Summary

Over 2005 and 2006, the specific support action 'the European Lipidomics Initiative (ELIfe)' has
resulted in four successful workshops where stakeholders from basic science, industry and medicine
met to discuss cell biological, technological, industrial and clinical aspects of lipidomics. In
addition, ELIfe contributed lipidomics sessions to four networking meetings, one on life sciences,
one on the bioscience of lipids and two industrial lipid meetings. These workshops and meetings
culminated in the concluding general meeting in October 2006, attended by a broad audience of 250
scientists. At the occasion of this meeting a special journal issue was published on 'Lipidome and
Disease' [16]. The results of the the specific support action were reported in a series of policy
papers in the scientific literature [4-6, 8-13], and a policy briefing on lipidomics and health of the
European Science Foundation [14]. In addition, ELIfe contributed to a number of technical papers
on lipid classification and data handling [1-3] and to a 500 page document entitled 'Enabling
technologies for studying the genome, proteome and cytome of the lipidome.' [7]. This document
will be presented on the Lipidomics Expertise Wiki Portal (LEP-Wiki): http://www-cgi.uni-
regensburg.de/Klinik/Klinische_Chemie/lipidWiki/, to allow a direct interaction with the scientists
in the field. Interviews with ELIfe members appeared in various journals [17,18].

A survey was prepared based on registrations in the Lipidomics Expertise Platform
www.lipidomics-expertise.de with the purpose of identifying stakeholders and providing insight
in the available expertise, which can be used for contacting specific centers for collaboration. A 260
page document entitled 'Lipid droplets and lamellar bodies as dynamic organelles connecting influx,
efflux, and storage of lipids: Translational research towards human disease' was prepared as the
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basis for a grant proposal under FP7 by a number of ELIfe members plus other scientists. This
proposal 'LipidomicNet' was recently selected for funding. A EuroCore theme proposal under the
name EuroMembrane [15] has been accepted by the European Science Foundation and a call for
applications is expected for early 2008.

In summary, we believe that the European Lipidomics Initiative has created many opportunities for
crossover between basic science and medical and commercial applications, and that it has inspired
stakeholders to seek contact and establish strategic alliances. Because national funding agencies and
policy makers value the European dimension, the project will impact on both the European and the
national level in shaping policies and research activities, both in applied and fundamental research.
One such field is that of nutrition and health. Technology development will allow more detailed
analyses of lipid patterns in diseased and healthy persons, which will drive discussions with the
food industry concerning the potential positive and negative effects of different types of (lipid)
nutrition on human health with new health policies drawn up as a result.

References

[1.a]   Fahy, E., S. Subramaniam, H.A. Brown, C.K. Glass, A.H. Merrill, Jr., R.C. Murphy, C.R.
        Raetz, D.W. Russell, Y. Seyama, W. Shaw, T. Shimizu, F. Spener, G. van Meer, M.S.
        Vannieuwenhze, S.H. White, J. Witztum, and E.A. Dennis (2005) A comprehensive
        classification system for lipids. J. Lipid Res. 46, 839-861.
[1.b]   idem: (2005) Eur. J. Lipid Sci. Technol. 107, 337-364
[2]     Varfolomeyev, S., Efremenko, E., Beletskaya, I., Bertini, I., Blackburn, G.M., Bogdanov,
        A., Cunin, R., Eichler, J., Galaev, I., Gladyshev, V., O’Hagan, D., Haertle, T., Jarv, J.,
        Karyakin, A., Kurochkin, I., Mikolajczyk, M., Poroikov, V., Sakharov, I., Spener, F., Voyer,
        N., and Wild, J. (2005) Postgenomic chemistry (IUPAC Technical Report). Pure Appl.
        Chem. 77, 1641–1654.
[3]     Spener F. (2005) Lipidomics and consequences: a new classification system for lipids. Eur.
        J. Lipid Sci. Technol. 107, 277-278.
[4]     van Meer, G. (2005) Cellular Lipidomics. EMBO J. 24, 3159-3165.
[5]     Schmitz, G., Liebisch, G., Langmann, T. (2006) Lipidomic strategies to study structural and
        functional defects of ABC-transporters in cellular lipid trafficking. FEBS Lett. 580, 5597-
        5610.
[6]     van Meer, G., Leeflang, B.R., Liebisch, G,. Schmitz, G., Goni, F.M. (2007) The European
        lipidomics initiative: enabling technologies. Methods Enzymol. 2007;432, 213-232.
[7]     White paper on 'Enabling technologies for studying the genome, proteome and cytome of
        the lipidome.' to be published in Wikipedia format on the LEP website.
[8]     Spener F. (2005) European Commission funds lipidomics project. Eur. J. Lipid Sci. Technol.
        107, 1-2.
[9]     Griffiths, W. (2006) Why steroidomics in brain? Eur. J. Lipid Sci. Technol. 108, 707–708.
[10]    Helms, B. (2006) Host-Pathogen interactions: Lipids grease the way. Eur. J. Lipid Sci.
        Technol. 108, 895–897.
[11]    Spener, F., Kohlwein, S.D., and Schmitz, G. (2006) Lipid droplets and lamellar bodies –
        from innocent bystanders to prime targets of lipid research for combating human diseases.
        Eur. J. Lipid Sci. Technol. 108, 541-543.
[12]    Spener, F., Zechner, R., and Borlak, J. (2006) Is lipotoxicity an oxymoron? Eur. J. Lipid Sci.
        Technol. 108, 625-627.
[13]    van Meer, G. (2006) How do sphingolipids and lipid rafts relate to pathology? Eur. J. Lipid
        Sci. Technol. 108, 799–801.
[14]    van Meer, G. and Spener, F. (Co-Chairs), Leeflang, B.R. (Secretary), Beisiegel, U.,
        Bougnoux, P., Goñi, F., Griffiths, W., Hartmann, T., Helms, B., Hoekstra, D., Julià-Sapé,
        M., Larijani, B., Moschetta, A., Mouritsen, O.G., Norata, G.D., Payrastre, B., Record, M.,

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       Schmitz, G., Simons, K., Tselepis, A., Vaz, W., Vigh, L., Voelker, D.R., Wakelam, M.J.O.,
       and Wanders, R.J.A. (2008) Structural Medicine II: the Importance of Lipidomics for Health
       and Disease, European Science Foundation Policy Briefing, in press.
[15]   van Meer, G. (main proposer), Malhotra, V., Marsh, M., Simons, K., van der Goot, G.,
       Warren, G. (2008) Membrane Architecture and Dynamics (EuroMEMBRANE), EuroCORE
       theme proposal. Call to be launched in spring 2008.
[16]   Helms, B. and van Meer, G., eds. (2006) Lipidome and Disease. FEBS Letters Special Issue.
       FEBS Lett. 580, 5429-5610.
[17]   Winckler, L. (2005) Lipidomics. Laborjournal 12, 20-23.
[18]   Hillyer, C.D. (2006) Lipidomics: taking it one lipid at a time. Inform 17, 206-208.

Other:
ELIfe flyer
Advertorial in the Parliament Magazine, issue 210 of October 3, 2005, entitled "Fat is Bad?"

Survey:
The data in the Lipidomics Expertise Platform database have been analyzed and the survey will be
published on the website (see Annex 2 of the reports).




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                                  Qui ckT ime™ and a
                        T IFF (Uncompressed) decompressor
                            are needed to see thi s pi cture.




                                   LSSG-CT-2004-013032
                                             ELIfe
                    European Lipidomics Initiative: Shaping the Life Sciences

Instrument: Specific Support Action
Thematic Priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6




          TOWARDS A EUROPEAN LIPIDOMICS PROGRAM

                                 Appendix 13
                  Science and society reporting questionnaire
                                Appendix 15
            Final reporting questionnaire on workforce statistics
                                 Appendix 16
                     Socio-economic reporting questionnaire


                                                                Final Report
                                                                 2005-2007




Period covered: from 1/1/06 to 1/7/07                                            Date of preparation: 1/1/08
Start date of project: 1/1/05                                                    Duration: 2.5 years
Project coordinator name:                                         Prof. Gerrit van Meer
Project coordinator organisation name:                            Utrecht University

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Appendix 13 – Science and society reporting questionnaire
                              All projects
                                                                                ELIfe Final Report




Science and Society Reporting Questionnaire
Introduction
FP6 was designed to focus, integrate, structure and strengthen the European Research
Area (ERA). The influence of science and technology on society was acknowledged when
the ERA was established and the importance of having a healthy dialogue between
science and society was recognised. This area now forms part of the policy to structure
the ERA under the heading Science and Society. It incorporates ethical, gender and
communications issues together with issues affecting education and youth and
governance.

This questionnaire has been compiled for FP6 Project Coordinators. It has been
designed to help coordinators respond to contractual reporting requirements (Article
II.10.3 of the contract states that consortia must engage with actors beyond the research
community) and to facilitate the monitoring of the science and society dimension in FP6.

The information gathered through this exercise will be confidential and will not be
disclosed to any third parties or used in any way that could be linked to individual
projects.

Please complete the questionnaire by ticking boxes or filling out information where
requested. It would be appreciated if as many questions as possible could be completed.

Please note that Part A will be completed automatically when the contract number is
entered.


A    General Information on Contractor

1    Contract Number:                               LSSG-CT-2004-013032



2    Instrument:                      Specific Support Action

3    Thematic Priority:               FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6



4    Title of Project:                European Lipidomics Initiative:
                                           Shaping the Life Sciences

5    Name and Title of                Prof. G. van Meer
     Coordinator:


6    Period Covered, Start Date: 1/1/05                End Date: 1/7/07



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7    EC Contribution to project:      € 487,200



B    Ethics

8    Which (if any) of the following does your research project involve?
      Human beings
      Human biological samples
      Personal data
      Genetic information
      Animals
      Human embryos or human embryonic stem cells
      Non human primates and other animals
      None of the above


9    To what extent do you believe ethical issues are relevant to your research project?
       Not relevant
       Minor relevance
       Significant relevance
       Critical


10   Do you have Ethicists or others with considerable ethics experience involved in the
     project?
       Yes
       No


11   Did your project have a separate EC ethical review?
       Yes
       No


12   How much (including the value of time spent, as well as paid-out costs) do you
     estimate your project (when it is completed) will have spent on considering and
     dealing with ethical issues?
     €    5,000




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C     Gender (to be completed for all projects except IPs and NoEs)

13a Did you undertake Gender Equality Actions in your research project?
      Yes
      No


13b If no, why not?
      Not relevant
      Team not gender aware
      No budget
      Not supported (no will)
      Other:


13c If yes, which of the following actions did you carry out and how effective were
    they?
                                                           Not at all    Very
                                                            effective    effective
       Design and implement an equal opportunity policy           
       Implement mentoring schemes for women                     
       Family friendly working conditions                        

14    Was there a gender dimension associated with the research content?
       Yes. If yes, please specify
          No

15    How much (including the value of time spent, as well as paid-out costs) do you
      estimate your project (when it is completed) will have spent on considering and
      dealing with gender issues?
       €   -




D     Science Education, Training and Career Development

16a Does this project anticipate having a direct impact on the local economy?
      Yes
      No


16b If Yes, is the project:
      Stimulating employment
      Retaining highly trained personnel
      Creating possible spin-out/start-up companies




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17   Does your partnership employ and train researchers?
       Yes
       No


18   Does your project involve working with young people at schools?
       Yes
       No


19   Is there any education material being produced directly or indirectly by your
     project?
       Yes
       No


20   How much (including the value of time spent, as well as paid-out costs) do you
     estimate your project has spent on considering and dealing with Science
     Education, Training and Career Development issues? € 5000



E    Engaging With Actors Beyond the Research Community

20a Is the project likely to generate outputs (expertise or scientific advice) which could
    be used by policy makers?
      Yes
      No


20b If Yes, is this a primary or secondary objective of the project?
      Primary
      Secondary


21a Did your project engage in significant communication with the public before
    research commenced?
      Yes
      No


21b Was the focus or methodology of your project modified in response to any
    communication with the public?
      Yes
      No




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22   Does your project involve someone whose role is solely to communicate with the
     public?
       Yes
       No



F    Use and dissemination

23   How many articles were published ?
     In refereed journals:           13             Other journals:   5

24   How many patents have been applied for ?
                                 -

25   How many other Intellectual Property Rights were applied for?
                                 -

26   How many spin-offs were created? -


27   Have you issued press releases related to your project (and if so, how many)?
      Yes, number:          2
      No


28   Have you held media briefings? If so, how many, and on average roughly how
     many journalists attended?
      Yes, number of                      average number of journalists:
          briefings:
         No




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29a Roughly how many items covering your project in the printed press, on radio or
    television can you identify?


            Press:          21          Radio:                    Television:



29b Roughly how many items were:
        Specialist          18                                 Non-specialist                    3
           Press:                                                     Press:

         National            1                                   International                 20
           Press:                                                       Press:



30a Was there on-line information about the project?
     Yes
     Specific web site
     No

30b Roughly how frequently has it been updated?
           6 months




31    Do you have an e-mail mailing list to send news about the project? If so, how
      many subscribers to the list are there?
        Yes, number of subscribers:    2,500 scientists were addressed
        No


32a Have you created or participated in an event (e.g. workshop, conference,
    information day) in order to communicate with the public (not just other
    researchers or the press)?
      Yes
      No Except for the contributions to 2 lipid congresses that covered lipid commerce and
                     consumer demands


32b Roughly how many people attended these events and learned about your project?
           200




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33a Have you produced a video or DVD film about your project?
      Yes
      No

33b If so, how effective do you believe it has been in communicating with the public?
      Unable to assess
         Completely ineffective
         Mostly ineffective
         Partially effective
         Significantly effective
         Extremely effective



34a Have you produced posters, flyers or brochures about your project?
      Yes
      No

34b If so, how effective do you believe they have been in communicating with the
    public?
      Unable to assess
         Completely ineffective
         Mostly ineffective
         Partially effective
         Significantly effective
         Extremely effective



35   In how many different languages were these products (video/DVD, posters, flyers,
     brochures) produced?
          English



36   How have you distributed these products (video/DVD, posters, flyers, brochures)?
     Please tick all methods you have used.
       Sent on request
       Sent to schools/academic institutions
       Distributed through government agencies/public buildings/libraries etc.
       Sent to potentially interested non-governmental bodies (NGOs, citizen’s associations etc)
       Other: Spread through the mailings of international oragnizations: FEBS, EMBO, EuroFed
                             Lipid, ICBL




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G Total Communication Spend

37   How much (including the value of time spent, as well as paid-out costs) do you
     estimate your project (when it is completed) will have spent on communication
     activities (engaging with the public, use and dissemination) as described in the
     current questionnaire?
      €    63,600                                          12 months of EC funded efforts; mostly
                                                           publications, website and meeting organization




H Comments

38   If you have any comments about your experience of meeting the Science and
     Society objectives within your project, or any suggestions of improvements to the
     programme please add them here:

      In hindsight the initiative should have hired one full time person to handle the organizational aspects of
               the initiative and the broad public dissemination of the results.




                                       you for your heour help!

[Submission instructions will need to be elaborated by those that set up the questionnaire on
the Internet].




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Appendix 15 – Final reporting questionnaires on workforce statistics
                     All projects except IPs and NoEs




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                                                                                          ELIfe Final Report


                              WORK FORCE STATISTICS
                                 FINAL REPORT

This report is part of the final reporting to be completed by the contractors of all projects except IPs
and NoEs at the end of the project.


1. GENERAL INFORMATION

1.1. Contract No.: LSSG-CT-2004-013032

1.2. Thematic priority: FP6-2003-LIFESCIHEALTH-II; LSH-2003-3-6

1.3. Instrument type: Specific Support Action

1.4. Project acronym: ELIfe

1.5. Period covered (Start Date – End Date)1: 1/1/05 – 1/7/07

1.6. Name and title of co-ordinator1: Prof. G. van Meer

1.7. Name and title of contractor: Utrecht University


2. SCIENTIFIC LEADERSHIP AND MANAGEMENT, AND WORKFORCE STATISTICS FOR THE
PROJECT TO BE COMPLETED BY CONTRACTORS (SUMMARY TABLE)

       Please complete the table below on a Headcount basis (Previously supplied data will be inserted
       automatically)

                                             Number of           Number of                  %           %
 Type of Position                                                                 Total
                                             Women               Men                        Women       Men


 Scientific manager                           1                   1               2         50           50
 Scientific team leader / work package
 manager                                      2                   12              14        14           100
 Experienced researcher (> 4 years)          41                   68              109       38           62
 Early researcher (<= 4 years)
 PhD students
 Technical staff                              1                                   1         100
 Other




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                                                       ELIfe Final Report




Appendix 16 – Socio-economic reporting questionnaire
                              All projects




                                                                    105
                       SOCIO-ECONOMIC REPORTING QUESTIONNAIRE
                                       SUMMARY STATEMENT BY COORDINATOR


INTRODUCTION

In the process of building the European Research Area, democratic governance must ensure that social and
economic issues are taken into consideration in the research activities and that citizens are informed about
and aware of the social aspects with regard to scientific and technological progress. In this context, it is also
acknowledged that the benefits of research in support of socio-economic policy challenges would be
enhanced by an appropriate integration of socio-economic research dimensions.

The importance of the integration of socio-economic aspects in research was recognised in FP6 and should
be duly taken into consideration by contractors where relevant for the actions concerned in horizontal and
thematic activities of FP6.

This questionnaire applies to all projects and must be filled in by each contractor in the project. It is designed
to facilitate the monitoring of the integration of the socio-economic dimensions in FP6 and to finally support
the assessment of the research that will guide the future policy formulations and decisions.

The submission of this questionnaire will be done on-line. The details of the procedure to be used will be
communicated by the Commission to the project coordinator in due time.

The information gathered through this exercise will be kept confidential and will not be disclosed to any
third parties or used in any way that could be linked to individual projects.

QUESTIONS

1.1 Do your tasks in the project include socio-economic research activities1 ?                                 N/A


1.2 If “Yes”, what is the estimated total budget allocation that addresses                                    N/A
    these activities ?



1
    - Ex-ante or ex-post assessments (or contribution to such analysis e.g. cost-benefit/cost-effectiveness studies, etc…) of
     the expected impact of the knowledge and/or technology generated from the research (project, programme or
     framework programme), as well as analysis of the factors that would influence their exploitation (e.g. statistical
     indicators, standardisation, ethical and regulatory aspects, impact on consumers and markets, public
     awareness/acceptance and understanding of science, political/societal and/or economic implications, etc…)

    - Any type of models or tools to support the assessment of impact on society, economy and businesses resulting from
    the deployment of new services or technologies.

    - Any research seeking both a better integration of Science in Society and Society in Science.

    - Any type of research aiming at understanding the societal and economic phenomena (research in social sciences and
    humanities)

    - Actions e.g. assessments, tools & methods, comparative research, etc to support the formulation and implementation
    of Community policies.

    - Any type of activity involving scientist(s) with a specific background in social, political sciences or in economy
    (discipline approach).
                                                                                                           ELIfe Final Report




2.1 Do your tasks in the project include foresight methods2 ?                                               Not really

Only expected needs and developments in networking, technology and bioinformatics were discussed.

2.2 If “Yes”, what is the estimated total budget allocation that addresses                                      N/A
    these activities?


3. How many person/months (estimated) were allocated to researchers
                                                                                                               None
   with a background in social sciences3, to perform your tasks for
   the project ?




2
    - Any type of foresight, i.e. participative vision-building approaches, future studies and forward looking activities,
     including scenarios of the evolution of Europe’s potential in a related field, forecasting, prospective studies, forward
     looks, etc.
3
    Domains of academic disciplines covered by the social sciences are: Psychology, Economics, Education sciences,
    Anthropology (social and cultural) and ethnology, Demography, Geography (human, economic and social), Town
    and country planning, Management, Law, Linguistics, Political sciences, Sociology, Organisation and methods,
    Miscellaneous social sciences and interdisciplinary.


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