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Curriculum Vitae for Timothy A. Cross, Professor of Chemistry &

NMR Spectroscopy & Imaging Program Director, National High Magnetic Field Laboratory

1800 E. Paul Dirac Dr., Tallahassee, FL 32306-4005



Education:

Trinity College Hartford, CT B.S. 1976 Chemistry

University of Penn. Philadelphia, PA Ph.D. 1981 Chemistry



Positions:

Department of Chemistry, University of Pennsylvania

Postdoctoral Fellow with Stanley J. Opella 1981-1983

Department of Biophysical Chemistry, Biozentrum der Universitat Basel

Postdoctoral Fellow with Joachim Seelig 1983-1984

Department of Chemistry, Florida State University

Assistant Professor 1984-1989

Associate Professor 1989-1993

Professor 1993-

Director of the FSU Nuclear Magnetic Resonance Facility 1984-

Department of Biological Science

Courtesy Associate Professor 1997-

Institute of Molecular Biophysics, Florida State University 1984-

Director of the Institute & Structural Biology Program 1991-1993

Center for Materials Res. & Tech., Florida State University 1990-

National High Magnetic Field Lab., Florida State University 1990-

Acting Director NMR Program 1991-1994

Director for New Initiatives 1995- 1997

Director of the NMR Program 1997-



Honors:

Phi Beta Kappa 1975-

Honors in Chemistry and General Scholarship 1976

Mary A. Terry Fellowship 1976-1978

Meritorious Teaching Awards 1977, 1978

NIH Predoctoral Trainee in Cell and Molecular Biology 1978-1981

NATO Postdoctoral Fellowship 1983-1984

NSF Presidential Young Investigator Award 1985-1990

Alfred P. Sloan Research Fellow 1988-1990

Florida State University Developing Scholar Award 1990-1991

Fellow of the American Academy for the Advancement of Science 1997-



Service:

NIH Molecular Cytology Study Section (ad hoc) 1988

NSF ILI Review Pannel 1989

NSF Biophysics Review Panel 1989-1992

NIH Shared Instrumentation Panel 1993

NIH Special Study Section 1993

NIH BBCB Study Section 1995-1999

Advisory Board - Magnetic Resonance in Chemistry 1997-2000

Editorial Board - J. Magnetic Resonance 1998-2000

Editorial Board - Biophysical Journal 1998-2001

Biophysical Society Council 2000-2003



5 Publications Most Closely Related to the Proposed Project:

Ketchem, R.R., Roux, B. & Cross, T.A. (1997) “High-Resolution Polypeptide Structure in a Lamellar

Phase Lipid Environment from Solid-State NMR Derived Orientational Constraints” Structure

5:1655-1669.

Fu, R. & Cross, T.A. (1999) “Solid-State NMR Investigation of Protein and Polypeptide Structure”

Annu. Rev. Biophys. Biomol. Struct. 28:235-268.

Fu, R., Cotten, M. & Cross, T.A. (2000) “Inter- and Intramolecular Distance Measurements by Solid

State Magic Angle Spinning NMR: Determination of Gramicidin A Channel Dimer Structure in

Hydrated Phospholipid Bilayers” J. Biomol. NMR 16:261-268.

Kovacs, F.A., Denny, J.K., Song, Z., Quine, J.R. & Cross, T.A. (2000) “Helix Tilt of the M2

Transmembrane Protein: An Intrinsic Property of the Protein” J. Mol. Biol. 295:117-125.

Wang, J., Denny, J.K., Tian, C., Kovacs, F.A., Song, Z., Fu, R., Quine, J.R. & Cross, T.A. (2000)

“Orientation of Membrane Protein Helical Wheels without Resonance Assignments from

PISEMA” J. Magn. Reson. 144:162-167.



5 Most Important Publications:

Arumugam, S., Pascal, S., North, C.L., Hu, W., Lee, K.-C., Cotten, M., Ketchem, R.R., Xu, F.,

Brenneman, M., Kovacs, F., Tian, F., Wang, A., Huo, S. and Cross, T.A. (1996) “Conformational

Trapping in a Membrane Environment: A Regulatory Mechanism for Protein Activity?” Proc.

Natl. Acad. Sci. U.S.A. 93:5872-5876.

Tian, F. & Cross, T.A. (1999) "Cation Transport: An Example of Structural Based Selectivity" J.

Mol. Biol. 285:1993-2003.

Xu, F & Cross, T.A. (1999) "Water: A "Foldase" that Catalyzes Hydrogen Bond Exchange in

Polypeptide Conformational Rearrangements" Proc. Natl. Acad. Sci. U.S.A. 96:9057-9061.

Kovacs, F., Quine, J. & Cross, T.A. (1999) “Validation of the Single-Stranded Channel Conformation

of Gramicidin A by Solid-State NMR” Proc. Natl. Acad. Sci. U.S.A. 96:7910-7915.

Cross, T.A., Arseniev, A., Cornell, B.A., Davis, J.H., Killian, J.A., Koeppe II, R.E., Nicholson, L.K.,

Separovic, F., & Wallace, B.A. (1999) “Gramicidin Channel Controversy - Revisited” Natur. Struct.

Biol. 6:610-611.



Collaborators:

Tetsuo Asakura, Tokyo Univ. of Agriculture & Technology; David Busath, Brigham Young University;

Larry Pinto, Northwestern University; Robert Lamb, Northwestern University; Christopher Miller,

Brandeis University; Ronald Kaback, UCLA



Graduate Students During the Past 5 years (out of a total of 23):

Myriam Cotten 8/92-12/98 PhD

Feng Xu 8/92-11/97 PhD

Fang Tian 8/92-5/98 PhD

Frank Kovacs 8/92-6/99 PhD

Junfeng Wang 8/95-

Zhang, Li 8/96-11/98 M.S.

Tian, Changlin 8/97-

Mo, Yiming 8/98-

Kim, Sanguk 8/98-

Hu, Jun 8/99-



Postdoctoral Fellows During the Past 5 years (out of a total of 8):

Dr. Anping Wang (1/95-1/96); Dr. Victoria Soghomonian (6/95-12/98); Dr. Zhiyan Song (7/97-

6/99); Dr. Katsuyuki Nishimura (5/99- )



Graduate & Postdoctoral Supervisors:

Stanley J. Opella, University of Pennsylvania

Joachim Seelig, University of Basel, Switzerland



Present Funding Support:

NIH AI-23007-14 “Correlations: Structure-Dynamics-Functional in Channels” Annual Direct Costs

„00-„01- $300k, 3/00 – 2/05, (Cross, PI; Fu, Co-PI; Busath, Co-PI).

NSF MCB 9986036 “Solid State NMR-derived Structure: Backbone of the Influenza A M2 Protein”

Total Annual Direct Costs - $95k, 2/00 – 1/03, (Cross, PI; Fu, Co-PI; Quine, Co-PI).

NSF DMR 9603935 “830 MHz Spectrometer for the National High Magnetic Field Lab.” Total

Direct Costs - $558k, 2/98 – 1/01, (Cross, PI; Edison, Co-PI; Gibbs, Co-PI; Markiewicz, Co-PI).



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