D2-40 Lymphatic Endothelial Marker Monoclonal Antibody_ Purified

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					D2-40 Lymphatic Endothelial Marker
Monoclonal Antibody, Purified (SIGNET)
Catalog Number: SIG-3730
Available Size: 0.1 mL, 1.0 mL, L1
6mL, L2 6mL

Description: Purified Monoclonal Antibody
against Lymphatic Endothelial Marker
(Formerly Signet Catalog Nos: 730-001, 730-
01, 730-16 L1 Predilute, 730-26 L2 Predilute)

Intended Use: ** In Vitro Diagnostic (IVD) **
                                                                    Image Description: Immunohistochemistry showing
Clone: D2-40                                                        lymphatic channels with staining of the endothelium.
                                                                    The adjacent small blood vessels show no staining of
Form: Purified Antibody (in 0.01M PBS + 0.1%                        their endothelium. The section is from muscularis
NaN3 + 1% BSA)                                                      propria of bowel (40X).

Host: Mouse                                                         Both are ready-to-use with either a Biotin-
                                                                    based (USA Ultrastreptavidin Detection, SIG-
IsoType: IgG1                                                       32250) or Biotin-free (Acuity Polymer
                                                                    Detection, SIG-32602) detection systems.
Species Reactivity: Human
                                                                    Uses: This antibody is effective in
Specificity: Clone D2-40 reacts with a O-linked                     immunoblotting and immunohistochemistry
sialoglycoprotein (MW 40kDa) found on                               (IHC).
lymphatic endothelium, fetal testis and on the
surface of testicular germ cell tumors. In                          Suggested Working Dilution: The optimal
recent studies clone D2-40 (D240) has shown                         working dilution should be determined for each
staining in lymphatic channel endothelium but                       specific assay condition.
not in the adjacent capillary.                                          ▪ Western blot:
                                                                            0.1 - 0.5 ug/mL
This antibody was developed using M2A.                                  ▪ IHC: ≥1:40 (concentrated
                                                                           format) for Biotin based
Concentrated Format:                                                       detection systems such as
SIG-3730-100 [0.1 mL]                                                      Signet’s USA Ultrastreptavidin
SIG-3730-1000 [1 mL]                                                       Detection (SIG-32250).
                                                                    Tissue Sections: Formalin-fixed, paraffin-
Prediluted Formats:                                                 embedded tissues, frozen sections
SIG-3730-16 [6 mL]                                                  Pretreatment: Not required
SIG-3730-26 [6 mL]                                                  Incubation: 60 minutes at room temperature

                                   5858 Horton Street, Suite 500 • Emeryville, California • 94608
                (p) 800 / 922-2226 • (f) 888 / 320-4837 • (e) ab.products@CRPinc.com • (w) http://www.CRPinc.com
                                                                Page 1 of 2
Cont. of SIG-3730

Storage: Store between 2-8°C.

References: Choi, WW, et al. Angiogenic and
lymphangiogenic microvessel density in breast
carcinoma: correlation with clinicopathologic
parameters and VEGF family gene expression.                             Giorgadze TA, et al. Lymphatic vessel density
Mod Pathol 18:143-52, 2005.                                             is significantly increased in melanoma. J Cutan
                                                                        Pathol 31:672-677, 2004.
Chu, AY, et al. Utility of D2-40, a novel
mesothelial marker, in the diagnosis of                                 Warranty/Conditions: Covance products may
malignant mesothelioma. Mod Pathol 18:105-                              not be resold or modified for resale without
110, 2005.                                                              prior written approval.

Dumoff KL, et al. Low D2-40 immunoreactivity                            Related Products:
correlates with lymphatic invasion and nodal
metastasis in early-stage squamous cell                                      ▪   ACUITY Biotin Free Polymer Detection HRP
carcinoma of the uterine cervix. Mod Pathol                                      System 50 Test DAB (SIGNET)
18:97-104, 2005.                                                                 Catalog Number SIG-32602

Galambos C, Nodit L. Identification of                                       ▪   Ultrastreptavidin (USA) Horseradish
lymphatic endothelium in pediatric vascular                                      peroxidase (HRP) 50 Test, Multi-Species, DAB
tumors and malformations. Ped Dev Pathol                                         Detection Kit (SIGNET)
8(2):181-9, 2005.                                                                Catalog Number SIG-32250

Fogt F, et al. Identification of lymphatic vessels
in malignant, adenomatous and normal colonic                            Product Revision Date: 1/11/2008
mucosa using the novel immunostain D2-40.
Oncol Rep 11:47-50, 2004.

Fogt F, et al. Observation of lymphatic vessels
in orbital fat of patients with inflammatory
conditions: A form fruste of
lymphangiogenesis? Int J Molec Med 13:681-
683, 2004.

Franchi A, et al. Tumor lymphangiogenesis in
head and neck squamous cell carcinoma.
Cancer 101(5):973-978, 2004.

 Franke FE, et al. Hobnail hemangiomas
(targetoid hemosiderotic hemangiomas) are
true lymphangiomas. J Cutan Pathol 31:362-
367, 2004.

                                       5858 Horton Street, Suite 500 • Emeryville, California • 94608
                    (p) 800 / 922-2226 • (f) 888 / 320-4837 • (e) ab.products@CRPinc.com • (w) http://www.CRPinc.com
                                                                    Page 2 of 2