AP Biology Lac Operon Worksheet 12-1-06 by 5q0n3ur2


									AP Biology Lac Operon Worksheet 12-1-06


   1. Explain the function of regulatory proteins in bacteria.
   2. Who were Jacob and Monod and what did they accomplish?
   3. Explain what an operon is.
   4. Explain what happens when the genes in an operon are transcribed.
   5. Explain the concept of polycistrony.
   6. How many genes in the lac operon and what are their functions?
   7. Explain the function of the operator.
   8. By analogy explain the functions of the promoter and operator.
   9. Explain the function of the LacI gene.
   10. Explain why the Lac I protein is called a repressor.
   11. Explain what happens in the case of a lack of lactose.
   12. Explain the concept of negative regulation.
   13. Explain what allolactose is and its general function.
   14. What happens when allolactose binds to the allosteric site on the repressor
   15. How does this process benefit RNA polymerase?
   16. Explain why allolactose is called an inducer.
   17. Give the function of the enzymes produced by the lac operon.
   18. At what point does the bacterial cell start to transcribe lac genes and why?
   19. What happens to this process when glucose is present?
   20. When does maximum production of lac operon occur and how?
   21. Explain the relationship between cyclic AMP and the concentration of glucose.
   22. What happens when there is no glucose and plenty of lactose?
   23. What affect does the binding of cAMP and the catabolite activator protein to the
       lac promoter have in the bacterial cell?
   ** do the self quiz until you understand this process

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