Ginger ingredients inhibit the d

							Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in
rat chemical hepatocarcinogenesis model
Author(s): Mansour MA (Mansour, Mahmoud A.)1, Bekheet SA (Bekheet, Saleh A.)1, Al-Rejaie SS
(Al-Rejaie, Salim S.)1, Al-Shabanah OA (Al-Shabanah, Othman A.)1, Al-Howiriny TA (Al-Howiriny,
Tawfeq A.)2, Al-Rikabi AC (Al-Rikabi, Ammar C.)3, Abdo AA (Abdo, Ayman A.)4
Source: BIOFACTORS Volume: 36 Issue: 6 Pages: 483-490 Published: NOV-DEC 2010
Department: Pharmacology
Abstract: To investigate the possible antitumor activity of ginger extract against hepatic
carcinogenesis initiated by diethylnitrosoamines (DEN) and promoted by carbon tetrachloride (CCl4).
A total of 60 mate Wistar albino rats were divided into four groups with 15 animals in each group. Rats
in group 1 (control group) received a single intraperitoneal (i.p.) injection of normal saline. Animals in
group 2 were given ginger (50 mg/kg/day) in drinking water for 8 weeks. Rats in group 3 (DEN group)
were injected with a single dose of DEN (200 mg/kg, i.p.), 2 weeks later received a single dose of CCl4
(2 mL/kg i.g) by gavage as 1:1 dilution in corn oil. Animals in group 4 (DEN-ginger group) received
the same carcinogenesis induction protocol as in group 3 plus ginger (50 mg/kg/day) in drinking water
for 2 weeks before induction of hepatocarcinogenesis and continued throughout the experimental
period. DEN-initiated and CCl4-promoted hepatocarcinogenesis in male Wistar rats was manifested
biochemically by elevation of serum hepatic tumor markers tested; alpha-fetoprotein and
carcinoembryonic antigen. In addition, hepatocarcinogenesis was further confirmed by a significant
increase in hepatic tissue growth factors; vascular endothelial growth factor, basic fibroblast growth
factor, and hydroxyproline content. A marked decrease in endostatin and metallothonein were also
observed. Long-term ginger extract administration 2 weeks before induction of hepatocarcinogenesis
and throughout the experimental period prevented the decrease of the hepatic content of
metallothionein and endostatin and the increase in the growth factors induced by the carcinogen.
Moreover, ginger extract normalize serum hepatic tumor markers. Histopathological examination of
liver tissue also correlated with the biochemical observations. These findings suggest a protective
effect of ginger extract against premalignant stages of liver cancer in the DEN-initiated and CCl4-
promoted hepatocarcinogenesis model in rats. (C) 2010 International Union of Biochemistry and
Molecular Biology, Inc. Volume 36, Number 6, November/December 2010, Pages 483-490. E-mail:
mansour196ous@hotmail.com
Document Type: Article
Language: English
Author Keywords: ginger ingredients; hepatocarcinogenesis; tumor markers; diethylnitrosoamines
Reprint Address: Mansour, MA (reprint author), King Saud Univ, Coll Pharm, Dept Pharmacol, POB
2457, Riyadh 11451, Saudi Arabia
E-mail Addresses: mansour1960us@hotmail.com
Funding Acknowledgement:
Funding Agency                                                   Grant Number
Research Center College of Pharmacy, King Saud University CPRC 229

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Publisher: IOS PRESS, NIEUWE HEMWEG 6B, 1013 BG AMSTERDAM, NETHERLANDS
IDS Number: 703PH
ISSN: 0951-6433
DOI: 10.1002/biof.122