Role of lactoperoxidase in mastitis and its clinical application Ming-Han Chiang and Shou-Cheng Wu Department of Biotechnology, NCTU Mastitis is an infectious disease that may spread over a dairy herd and severely affect the production and quality of milk. Because the symptom in cows with subclinical mastitis is subtle, it is often ignored leaving cows without treatment. Bacteriological sampling and milk somatic cell count (SCC) has been recommended by the International Dairy Federation (IDF) to be a standard procedure for the diagnosis of mastitis but not practical as a routine test. The major purpose of this report was to test whether lactoperoxidase (LPO), a milk enzyme secreted from the epithelial layer of mammary gland, might be overexpressed in milk of mastitis. Milk samples selected from 46 dairy cows were separated into four groups according to SCC as group A: <100 (n = 25), B: 100-200 (n = 6), C: 201-500 (n = 5), D: >500×103 (n = 10) cells/mL. We demonstrated that LPO was not only highly expressed in milk, but also positively correlated with the severity of mastitis. In addition, we showed that macrophages of somatic cells also expressed LPO as determined by RT-PCR and immunostaining. The experiment is now in progress to analyze the significance of LPO present in the milk using purified LPO via HPLC to conduct its anti-bacterial activity. In summary, LPO is a novel biomarker for the diagnosis of bovine mastitis.
Pages to are hidden for
"25"Please download to view full document