Autoclave use by mmcsx

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									                                         AUTOCLAVE USE
PURPOSE

To operate the:
Bench top Autoclave BA852 Serial number 0690/643
Touchclave II Autoclave Sterilising Machine: Type TC/435/KE. Serial Number L6762
Using pre-set programs for the sterilisation of media, laboratory equipment, and decontaminating
waste.


OTHER DOCUMENTS
None

RESPONSIBLE
Hermino Mansojuibier of the Prof. C. J. Schofield group.
Only trained personnel must operate the autoclave.
SAFETY
General laboratory safety procedures apply.
Heat protective gloves should be worn when handling hot materials.
A facemask should be used for the protection of volatiles.
The autoclave should not be opened until the pressure indicates zero bar and the temperature is below
80oC.

PROCEDURE

1       INTRODUCTION
Items of laboratory equipment and media to be used for bacterial cell growth and in tissue culture
requires sterilisation before use, this can be achieved using steam sterilisation using the Touchclave II
Autoclave, or the bench top autoclave, both detailed above.
Biohazard laboratory waste produced by Bio-organic research groups in the Chemistry Research
Laboratory must be deactivating before disposal. Steam sterilisation is used to achieve this using the
TE Touchclave Autoclave detailed above, located in the Fermentation Room (Room LG 10.037).
There is only one programme available for use in the bench top autoclave.
There are two programs available for use in the Touchcalve II Autoclave, one has a slow purge, which
is used for sterilisation of liquid such as media, and the other has a fast purge suitable for dry laboratory
equipment such as sterilisation of laboratory equipment and decontaminating waste.
Note: The bench top autoclave reservoir should only be filled with deionised water.
        The Touchclave II Autoclave reservoir should only be filled with deionised water.

2      MATERIALS

Bio-organic waste material or equipment.

3      DEFINITIONS

None

4      METHOD

Refer to procedure for the appropriate autoclave.
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           Generic Risk Assessment Form for Research Departments
  Important: Before carrying out the assessment, read the Guidance Notes associated with this form.

Title Use of autoclaves


Department        Chemistry                      Room Number            LG5 (10.037)
Name of           Wendy Sobey                    Date of Original       23 November 2005
Assessor                                         Assessment


Brief Description of Procedure / Activity and its Location:
Assessed procedures:

1) Loading material into the autoclave

2) Opening the autoclave after sterilisation

3) Removing items from the autoclave

4) Autoclaving volatile waste




Note: should no potential hazard be identified as associated with the procedure/activity in this location,
you may stop the risk assessment at this point.




                                                                                                         2
Laboratory Control Measures which will be applied to reduce the risk to an
acceptable level:

  All personnel are appropriately trained and follow the written procedure for the appropriate
  autoclave. All personnel working in the laboratories are required to wear protective clothing
  (gloves, lab coat and face mask if required) when working and observe good laboratory practice.




Additional hazards Involved:

    Substance/Item of
  equipment/procedure           Associated hazards         Existing Control Measures
   or physical location

  Steam                         Burning                    Care should be taken when opening the autoclave door at
                                                           the end of a cycle. Wait until the temperature has fallen to
                                                           below 40C. Stand back when the door is opened.

  Explosion of tightly sealed   Injury from glass and      Ensure bottles containing liquid are loosely sealed and that
  bottles containing liquid     burning                    such items are run on slow-purge cycles.


  Lifting baskets filled with   Physical damage to back.   Do not fill baskets with heavy items before lifting into
  items into or out of the                                 autoclave. Load or unload such items from the in place
  autoclave                                                baskets.

  Removal of hot equipment      Burning                    Use heat resistant gloves, which are available in the
  from autoclave                                           Fermentation Room.


  Explosion                     Flying shrapnel injuries   Annual maintenance contract and pressure testing
                                and steam burns




                                                                                                                          3
Persons Potentially at Risk:
All personnel using the autoclave.
Appropriate training, protective clothing and observation of good laboratory practice will minimise
these risks.




Action in event of an accident or emergency:

Steam burn
Run affected area under cold water for at least 15 mins. Contact first-aider

Back Injury
Contact first-aider.

Inhalation of volatiles
Remove victim to fresh air. If victim is not breathing give artificial respiration. If breathing is
difficult give oxygen. Contact first-aider.

Skin contact with volatiles
Flush with copious amounts of water for at least 15 mins. Remove contaminated clothing and
shoes. Contact first aider

Ingestion of volatiles
Wash mouth with water if conscious. Do not induce vomiting. Contact a first-aider

Flying Shrapnel wounds
Contact first-aider, do not remove shrapnel from wound.



Arrangements for monitoring Effectiveness of Control:
A service contract is in place with TLE, for a three point check on the autoclave, to be performed
on a six monthly basis.
Conducting spore testing on a six monthly basis performs validation of the autoclave.




                                                                                                      4
Review of the Risk Assessment:

Date of review                          Name of reviewer

Date of next review                     Signature



Have the control measures been effective in controlling the risk?

                           Yes            No


Have there been any changes in the procedure or in the information available, which affect the
estimated level of risk from the listed substances

                           Yes            No



What changes to the control measures are required?
Ensure that service contract is in place and validation checks have been made at the prescribed
intervals




                                                                                                  5
Receipt of Risk Assessment: [Guidance note 11]
This assessment has been issued to and read by:


           Name                    Date Read      Signature   Name and Signature of Trainer   Date of Training




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