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MMP-iresGFPneo

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					            Structure of MMP-iresGFPneo
                      Genome length 4294 nt, without transgene
                               Capacity ~4,100 bp

            LTR                                                  LTR
                          Y               Transgene
                                                       GFP neo              Provirus
                                                IRES
                        SD           SA
              m7G-
                         Y                Transgene
                                                       GFP neo          -An RNA Genome
                                                IRES
                        SD           SA

                                          Transgene
                              m7G-                                     -An Spliced Message
                                                IRES
                                                       GFP neo
MCS- multiple cloning site
m7G- 7-methyl guanosine cap

SD- splice donor
SA- splice acceptor
LTR- long terminal repeats
An- poly-A tail
Y- packaging signal

				
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posted:12/19/2011
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