Structure of MMP-iresGFPneo
Genome length 4294 nt, without transgene
Capacity ~4,100 bp
LTR LTR
Y Transgene
GFP neo Provirus
IRES
SD SA
m7G-
Y Transgene
GFP neo -An RNA Genome
IRES
SD SA
Transgene
m7G- -An Spliced Message
IRES
GFP neo
MCS- multiple cloning site
m7G- 7-methyl guanosine cap
SD- splice donor
SA- splice acceptor
LTR- long terminal repeats
An- poly-A tail
Y- packaging signal