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					Next Generation
Sequencing Services

                                                                          Whole Transcriptome Analysis
                                                                          Identify splice variants, discover novel
                                                                          exons, quantify transcripts, etc.

                                                                          Targeted Resequencing/Exome
                                                                          Identify SNPs and indels in a genomic
                                                                          region across multiple samples

                                                                          Small RNA Analysis
                                                                          Discover and quantify microRNAs

                                                                          Methylation Analysis
                                                                          Profile methylation sites across the

                                                                          Whole Genome Resequencing
                                                                          Explore SNPs and Indels in genomes of
 Start-to-Finish Solution                                                 Chromatin and RNA Immunoprecipitation
 EdgeBio has a team of experienced research professionals                 Sequencing (ChIP-Seq)/(RIP-Seq)
                                                                          Discover and quantify sites of protein-
 that are knowledgeable in next generation sequencing                     DNA and protein-RNA interactions
 technologies and applications. From library preparation
 through data analysis, EdgeBio representatives are there to             Bioinformatics
 discuss your project on a custom basis to ensure that you
                                                                          Data Analysis and Visualization
 receive the results you need for your research.                          Mapping and DeNovo Assembly
                                                                          Variant Analysis and Annotation
     Consultation on experimental design
     Sample QC and Library preparation                                    Open Source and Commercial Software
     Data analysis and customized bioinformatics support                  LIMS and Databases

     Preferred Service Provider for Applied BioSystems SOLiD              Development
                                                                          Specialized Algorithms
                                                                          Customized Data Analysis Pipelines

                                       For more information e-mail us:          US: 1-800-326-2685
                                                   Intl: ++1-301-990-0881
                                                                                Fax: 1-301-990-0881
                                                 or visit us at:                201 Perry Parkway, Suite 5
                                                Gaithersburg, MD 20877
Next Generation
Sequencing Services

Applications Offered
Whole Transcriptome Analysis
Transcriptome analysis is of growing importance in understanding how altered expression of genetic variants contributes to complex
diseases such as cancer, diabetes, and heart disease. Analysis of genome-wide differential RNA expression, alternative splicing, and novel
transcript discovery provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate,
development, and disease progression.

Targeted Resequencing
High throughput sequencing has made it financially feasible to capture and interrogate specific regions of the genome, in a large number
of samples. Regions such as those identified by WGA studies, as well as exomes of affected and control subjects, can be effectively captured
and sequenced.

Small RNA Analysis
Small RNA has been shown to play critical roles in developmental timing, cell fate, tumor progression and neurogenesis. The study of small
RNAs with next generation sequencing is a hypothesis-neutral method, allowing for the discovery and profiling of small RNAs without the
limitations and bias of microarrays.

Whole Genome Resequencing
Genomes of all sizes, from microbes to mammals and plants, can be sequenced at a reasonable price with high throughput sequencing.
Structural variation including SNPs and Indels can be identified and catalogued.

Methylation Analysis
The involvement of epigenetic inheritance in development is a large area of study. DNA Methylation is essential for proper mammalian
embryonic and postnatal development, and aberrant DNA methylation has been implicated in a number of diseases. High throughput se-
quencing has allowed for genome-wide comparison of methylation states in disease and normal samples, opening the door for large –scale
studies in many fields such as cancer, development, and infectious disease.

Chromatin and RNA Immunoprecipitation Sequencing (ChIP-Seq)/(RIP-Seq)
Determining how proteins interact with DNA or RNA to regulate gene expression is essential for fully understanding many biological pro-
cesses and disease states. Sequencing millions of ChIP- or RIP-enriched fragments using next generation sequencing technology enables
cost-effective and precise analysis of the binding sites of transcription factors, replication and transcriptional machinery, structural proteins
such as histones, ribonucleoprotein complexes, as well as the impact of protein modifications on genome occupancy.

Data analysis is a critical component of any Next Generation sequencing project. The Edge BioInformatics team can help you reduce the
time from data to results by offering customized bioinformatics solutions tailored to your specific needs for mutiple next generation se-
quencing platforms including 454, Illumina, and SOLiD.

                                                       For more information e-mail us:                            US: 1-800-326-2685
                                                                                     Intl: ++1-301-990-0881
                                                                                                                  Fax: 1-301-990-0881
                                                                  or visit us at:                                 201 Perry Parkway, Suite 5
                                                                                  Gaithersburg, MD 20877
Next Generation
Sequencing Services

Sampling of Recent Projects
Whole Transcriptome Analysis
    • Identification of imprinted genes contributing to specific brain regions by whole transcriptome sequencing
    • Genetic basis of virulence in bacterial pathogenesis through microbial reseq and transcriptome sequencing

Targeted Resequencing and Exome Sequencing
    •   Targeted resequencing of a region linked to Alzheimers Disease by GWAS in hundreds of individuals
    •   Identification of the genetic basis of colorectal cancer through exome sequencing
    •   Identification of the genetic basis of a novel syndrome with skeletal dysplasia through exome sequencing
    •   Identification of rare mitochondrial mutations linked to neurological disease through mitochondrial reseq

Small RNA Analysis
    •   miRNA discovery in human osteosarcoma
    •   miRNA profiling of 50 samples (case & control) in a preeclampsia study
    •   miRNA profiling in a post-traumatic stress syndrome animal model
    •   miRNA discovery in exosomes of oral cancer animal model

Methylation Analysis
   • Genome-wide study of methylation patterns in 1500 individuals to study schizophrenia.

Highlights and Summary
        •   Six (6) NextGen Sequencing Platforms Currently In Use
        •   Automated Sample Prep via Biomek FX & SciClone
        •   Dedicated In-House MD, PhD, and MS Level Informaticians
        •   >100 compute cores & >100TB storage
        •   Strategic Partnerships with CLCBio and Genologics (LIMS)
        •   Agilent SureSelect Certified Service Provider

EdgeBio, your trusted Next Gen Sequencing & Bioinformatics service provider.

                                               For more information e-mail us:             US: 1-800-326-2685
                                                              Intl: ++1-301-990-0881
                                                                                           Fax: 1-301-990-0881
                                                         or visit us at:                   201 Perry Parkway, Suite 5
                                                           Gaithersburg, MD 20877

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