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Preparing carbon coated grids Last updated 02/24/2006 Cut a sheet of mica to rectangle and notch upper left corner to keep track of top Attach a piece of scotch tape to mica and peel to obtain a smooth surface Place on Whatman filter paper in an inverted Petri dish Make a small paper tent See Carbon Evaporators protocol to make carbon film on mica Make Whatman paper slightly damp Label back side of mica Place in cold room for at least 30 minutes. Meanwhile attach Whatman to holey, metal disc with two paper clips and wet with distilled water Place on a stand in a glass dish and fill the glass dish with distilled water to about 1 cm above the Whatman and place the dish on a bed of paper towels Take a bunch of Maxtaform 300 mesh Cu/Rh grids out of acetone and arrange on a piece of Whatman Place a drop of dichloroethane+glue on each grid and allow it to dry Submerge the grids/Whatman onto the disc Slice off the end of a carbon coated mica to make a smooth edge Float carbon onto the water leading with the smooth edge Carefully aspirate water from dish while positioning the carbon into position above the grids by blowing gently and guiding with forceps. Take water to about 1 cm below the disc. Lift the disc, Whatman, and grids out of the dish.
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