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					              A               B                  C              D                E             F            G             H              I            J             K          L            M         N   O   P   Q   R   S   T   U   V   W
                                                          Partial plates
                                                          and/or pulled                                              Subadjacent
                                                            out plates                                                chromatin                                  Number       Total
                                                                                                                                                 Prophase or
                      Kinetochores with                   and/or fibrillar                   Small      Thin and/or decondensati                                    of     number of       Total
                                                                                                                                                prometaphase
                       triplate and mts Triplate with few strutcure at                    kinetochore    punctate    on and/or C- Metaphase                      sections kinetochores kinetochores
                                                                                                                                                             a
1 siRNA                     (normal)        or no mts      outer plates      Fuzzy ball      plates       plates    shaped plates plate present configuration     scored     counted       seen
2 CENP-I
              b,c
 3            1               8                  1                                                                                yes                               2           9
 4             2              6                                                                                                   yes                               3           6
 5             3              3                                                                                                   yes                               2           3
 6             4             10                                                                                                   yes                               2          10
 7             5                                                  6                                                               no                                2           6
 8             6                                                  4              4                                                no                                3           8
 9             7                                                                 8                                                no                                3           8
10             8                                 2                               1                                                no                                2           3
11             9                                 6                                                                                no                                3           6
12            10                                                  4                                                               no                                2           4
13            11                                                  3                                                               no                                2           3           66
14 NUF-2
15             1             11                                                                                                   yes                               2          11
16             2                                 2                6              6                                                no                                3          14
17             3                                 1                               9                                                no                                3          10
18             4                                 1                4              2                                                no                                3           7
19             5                                                                 6                                                no                                2           6
20             6                                                  4              3                                                no                                3           7
21             7                                                  5              7                                                no                                4          12
22             8                                                  5              3                                                no                                2           8
23             9              1                  3                2              2                                                no                                3           8
24            10                                                  3              3                                                no                                4           6
25            11                                 1                2              2                                                no                                2           5           94
26 CENP-C
27            1                                  2                                             1            3                     no                                2           6
28            2                                  4                                             2            4                     no                                3          10
29            3                                  3                                                          4                     no                                3           7
30            4                                  2                2                            1            4                     no                                3           9
31            5               2                  2                4                                         2                     no                                           10
32            6                                                   2              6                                                                   yes            3           8           50
33 BUB1
34            1                5                 1                                                                                yes                               3          6
35            2                6                                                                                                  yes                               3          6
36            3                9                                                                                                  yes                               3          9
37            4                6                                                                                                  yes                               3          6
38            5                9                                                                                                  yes                               2          9
39            6                2                                                                            3                     no                                3          5
40            7                3                                  2                                         1                     no                                3          6
                      diffuse chromatin
41            8       no k ID                                                                                                                        yes            4          0
                      diffuse chromatin
42            9       no K ID                                                                                                                        yes            5          0
                      diffuse chromatin
43            10      no K ID                                                                                                                        yes            5          0
44            11               2                 1                                                          3                     no                                2          6
45            12               2                 2                                                          1                     no                                3          5            58
46 hMis 12
47            1               4                  1                                                                                yes                               3          5
48            2                                                   2                                                       4                          yes            2          6
49            3               1                  1                                                                        4                          yes            3          6
50            4               2                  2                                                                        2                          yes            3          6            23
51 Aurora B
52            1              11                                                                                                   yes                               2          11
53            2                                                                                                            6                         yes            3           6
54            3                                                                                                            4                         yes            3           4
55            4                                                                                                           11      no                                3          11
56            5                                  5                                                                         4      no                                3           9
57            6                                  2                                                                         4      no                                3           6
58            6                                                   1                                                        7      no                                2           8
59            7                                                   1                                                        7      no                                2           8
60            8                                  2                2                                                        2      no                                3           6
61            9                                  1                2                                                        6      no                                3           9           78
62 CENP-F
63             1              2                                                                                                   no                                3          2
64             2              1                                                  1                                                no                                3          2
65             3                                                                 1                                                no                                2          1
66             4                                                                                                                  no                                4          0
67             5                                                                                                                  no                                3          0
68             6                                                                                                                  yes                               4          0
69             7                                                                                                                  telo                              2          0
70             8              1                                   2                                                               telo                              3          3
71             9                                                  1                                                               yes                               3          1
72            10                                                  2              1                                                                                  2          3            12
73                                                                                                                                                                                         381
     a
74     These cells display extensive undercondensed and unaligned chromosomes and nuclear envelope fragments.
     b
75     These numbers represent individual cells examined in siRNA-transfected samples.
     c
76     Data in red probably arise from untransfected cells or cells in which siRNA has not taken effects based on the presence of metaphase plate and largely normal kinetochores. These rows are, thus, not counted in Table II.

				
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