A B C D E F G H I J K L M N O P Q R S T U V W
Partial plates
and/or pulled Subadjacent
out plates chromatin Number Total
Prophase or
Kinetochores with and/or fibrillar Small Thin and/or decondensati of number of Total
prometaphase
triplate and mts Triplate with few strutcure at kinetochore punctate on and/or C- Metaphase sections kinetochores kinetochores
a
1 siRNA (normal) or no mts outer plates Fuzzy ball plates plates shaped plates plate present configuration scored counted seen
2 CENP-I
b,c
3 1 8 1 yes 2 9
4 2 6 yes 3 6
5 3 3 yes 2 3
6 4 10 yes 2 10
7 5 6 no 2 6
8 6 4 4 no 3 8
9 7 8 no 3 8
10 8 2 1 no 2 3
11 9 6 no 3 6
12 10 4 no 2 4
13 11 3 no 2 3 66
14 NUF-2
15 1 11 yes 2 11
16 2 2 6 6 no 3 14
17 3 1 9 no 3 10
18 4 1 4 2 no 3 7
19 5 6 no 2 6
20 6 4 3 no 3 7
21 7 5 7 no 4 12
22 8 5 3 no 2 8
23 9 1 3 2 2 no 3 8
24 10 3 3 no 4 6
25 11 1 2 2 no 2 5 94
26 CENP-C
27 1 2 1 3 no 2 6
28 2 4 2 4 no 3 10
29 3 3 4 no 3 7
30 4 2 2 1 4 no 3 9
31 5 2 2 4 2 no 10
32 6 2 6 yes 3 8 50
33 BUB1
34 1 5 1 yes 3 6
35 2 6 yes 3 6
36 3 9 yes 3 9
37 4 6 yes 3 6
38 5 9 yes 2 9
39 6 2 3 no 3 5
40 7 3 2 1 no 3 6
diffuse chromatin
41 8 no k ID yes 4 0
diffuse chromatin
42 9 no K ID yes 5 0
diffuse chromatin
43 10 no K ID yes 5 0
44 11 2 1 3 no 2 6
45 12 2 2 1 no 3 5 58
46 hMis 12
47 1 4 1 yes 3 5
48 2 2 4 yes 2 6
49 3 1 1 4 yes 3 6
50 4 2 2 2 yes 3 6 23
51 Aurora B
52 1 11 yes 2 11
53 2 6 yes 3 6
54 3 4 yes 3 4
55 4 11 no 3 11
56 5 5 4 no 3 9
57 6 2 4 no 3 6
58 6 1 7 no 2 8
59 7 1 7 no 2 8
60 8 2 2 2 no 3 6
61 9 1 2 6 no 3 9 78
62 CENP-F
63 1 2 no 3 2
64 2 1 1 no 3 2
65 3 1 no 2 1
66 4 no 4 0
67 5 no 3 0
68 6 yes 4 0
69 7 telo 2 0
70 8 1 2 telo 3 3
71 9 1 yes 3 1
72 10 2 1 2 3 12
73 381
a
74 These cells display extensive undercondensed and unaligned chromosomes and nuclear envelope fragments.
b
75 These numbers represent individual cells examined in siRNA-transfected samples.
c
76 Data in red probably arise from untransfected cells or cells in which siRNA has not taken effects based on the presence of metaphase plate and largely normal kinetochores. These rows are, thus, not counted in Table II.