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					 EFFECT OF INTRAVENOUS IMMUNOGLOBULINS (IVIG) ON
 MMP-9 AS A MECHANISM FOR METASTASES PREVENTION
      B. Gilburd1, N. Lahat2, S. Shapiro3, M. Blank1, S. Lev1, F. Kvapil1,
                         P. Fishman4, Y. Shoenfeld1
  1
   Dept of Medicine B, Center for Autoimmune Diseases, Chaim Sheba
 Medical Center, Tel-Hashomer, 2Carmel Medical Center and The Bruce
Rappaport Faculty of Medicine, Technion, Haifa, 3Carmel Medical Center,
 Haifa, 4Laboratory of Clinical and Tumor Immunology, The Felsenstein
   Medical Research Center, Rabin Medical Center Sackler Faculty of
           Medicine, Tel-Aviv University, Petach-Tikva, Israel
The matrix metalloproteinase 9(MMP-9) is associated with processes
involving cell migration, including tumor cells metastases, immune cell
mobility and angiogenesis . IVIG was recently reported to prevents tumor
metastasis in experimental animal models (Shoenfeld Y, Fishman P, Int
Immunology, 1999).The mechanism(s) by which MMP-9 is inhibited by
IVIG, whether directly or indirectly are not clear.The objective of our
research was to test the hypothesis that IVIG modulates MMP9 protein
secretion and m-RNA expression in monocytic cell line. Methods: U937
monocytic cell line was used in order to evaluate IVIG effect on MMP9.
Substrate specific zymography and northern blotting were performed to
evaluate the constitutive secretion and mRNA expression of MMP-9 and
the influence of IVIG on its activity and expression. Results: We have
observed that IVIG in dose dependent manner induced inhibition of
MMP-9 secretion and mRNA expression in U937 monocytic cell line.
Conclusion: Our results imply that IVIG may play a role in metastases
prevention by inhibition the activity and expression of MMP-9, which is a
key enzyme in cancer metastases.




Abstract Number: 205
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