Stem Cell Partners
A Regenerative Medicine Company
Stem Cell Partners LLC
A Regenerative Medicine Company
ENSURA-SEP
POINT OF CARE
CELL WASHING
C t t
Contact:
Lee Buckler
lbuckler@celltherapygroup.com
778.278.6311
778 278 6311
1
Introduction
The Ensura-Sep™ Canister is intended for the
washing of a cell suspension at the point of care.
Cell washing and cell concentration are achieved
in a single centrifugation step.
Technology is density phase washing with
proprietary centrifuge canister apparatus.
Potential applications include removal of DMSO,
Ficoll, enzymes, or red cell lysing reagents.
y y g g
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Summary of Cell Washing Method with Ensura-Sep:
Slide 1 of 2
Add density blocking
Ensura-Sep Tube Add cell suspension
reagent to vertical Pellet cells by centrifugation
fluid to reservoir chamber.
chamber. (no intermingling of fluid
phases occurs)
fluid (blue) density
Blocking fl id (bl e) has a densit that is less than cells (red circles)
but greater than cell suspending fluid (yellow).
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Summary of Cell Washing Method with Ensura-Sep:
Slide 2 of 2
6. Resuspend cells & harvest
5. Aspirate depleted fluid suspension cells by final aspiration into 5
from reservoir chamber. syringe.
Step 1: Add 2.4 mL of Density Blocking Reagent
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Step 2: Add 10 - 40 mL of cell suspension
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Step 3: Centrifuge (10 min for 2,000 x g)
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Step 4: Empty reservoir chamber
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Step 5: Harvest Cells (flushing to resuspend)
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Summary
Ensura Sep
Ensura-Sep Cell Canisters enable the
simple and rapid washing/concentration
of a cell suspension to be achieved in a
single centrifugation step.
We work with our clients to design
custom canisters and reagents to meet
their specific requirements.
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Stem Cell Partners
A Regenerative Medicine Company
Stem Cell Partners LLC
A Regenerative Medicine Company
ENSURA-SEP
POINT OF CARE
CELL WASHING
Contact:
Lee Buckler
lbuckler@celltherapygroup.com
778.278.6311
1