Systems of Mating:
the rules by which pairs of
gametes are chosen from the
local gene pool to be united in a
zygote with respect to a particular
locus or genetic system.
Systems of Mating:
A deme is not defined by geography but rather by
a shared system of mating. Depending upon the
geographical scale involved and the individuals’
dispersal and mating abilities, a deme may
correspond to the entire species or to a
subpopulation restricted to a small local region.
The Hardy-Weinberg model assumes one
particular system of mating – random mating –
but many other systems of mating exist.
Some Common Systems of Mating:
• Random Mating
• Inbreeding (mating between biological
relatives)
• Assortative Mating (preferential mating
between phenotypically similar individuals)
• Disassortative Mating (preferential mating
between phenotypically dissimilar
individuals)
Inbreeding: One Word, Several
Meanings
Inbreeding is mating between biological
relatives. Two individuals are related if
among the ancestors of the first individual
are one or more ancestors of the second
individual.
Inbreeding: One Word, Several
Meanings
• Inbreeding Can Be Measured by Identity by
Descent, Either for Individuals or for a Population
(Because of shared common ancestors, two
individuals could share genes at a locus that are
identical copies of a single ancestral gene)
• Inbreeding Can Be Measured by Deviations from
Random Mating in a Deme (either the tendency to
preferentially mate with relatives or to
preferentially avoid mating with relatives relative
to random mating)
Identity by Descent
Some alleles are identical because they
are replicated descendants of a single
ancestral allele
Pedigree Inbreeding, F
• Occurs when biological relatives mate
• Two individuals are related if among the ancestors
of the first individual are one or more ancestors of
the second individual.
• Because the father and the mother share a
common ancestor, they can both pass on copies of
a homologous gene that are identical by descent to
their offspring.
• Such offspring are said to be homozygous due to
identity by descent.
Pedigree Inbreeding Is Measured
by F = Probability of
Homozygosity due to Identity by
Descent at a Randomly Chosen
Autosomal Locus
F is Called the “Inbreeding
Coefficient”
Aa
A A
1 1
Simplify Pedigree 2 2
by Excluding
B C A B C A
Individuals
Who Cannot
Contribute to 1 1
Identity by 2 2
D Descent D
AA
(or aa)
Probability(D = AA) = ( 1/2)4 = 1/16
Probability(D=AA or D=aa) = 1/16 + 1/16 = 1/8
A'a' Aa
A' A A' A
1 1 1 1
Simplify Pedigree 2 2 2 2
by Splitting into
B C Mutually Exclusive
A' B C A' OR A B C A
Loops That Can
Contribute to 1 1 1 1
Identity by 2 2 2 2
D Descent D D
A'A' AA
(or a'a') (or aa)
Probability Identical by Descent = 1/8 + 1/8 = 1/4
F is calculated for individuals as a function of
their pedigree (e.g., Speke’s gazelle)
System of Mating refers to a
deme, not individuals.
Therefore, F is not a measure of
the system of mating.
This does not mean that pedigree
inbreeding has no population or
evolutionary implications.
F displays strong interactions with rare, recessive
alleles and epistatic gene complexes.
Consider first a model in which a recessive allele is lethal when
homozygous.
•B = the sum over all loci of the probability that a gamete drawn
from the gene pool bears a recessive lethal allele at a particular
locus.
•Alternatively, B = the average number of lethal alleles over all loci
borne by a gamete in the gene pool.
•BF = the rate of occurrence of both gametes bearing lethal alleles
that are identical by descent, thereby resulting in the death of the
inbred individual.
Consider first a model in which a recessive allele is
lethal when homozygous.
•The number of times an inbred individual will be identical-by-
descent for a lethal allele will often follow a Poisson distribution.
•e-BF = the probability that an individual has exactly 0 lethal genes
that are identical-by-descent and therefore homozygous.
•-A = the natural logarithm of the probability of not dying from any
cause other then being homozgyous for a lethal recessive allele that
is identical-by-descent, so e-A = the probability of not dying from
something else.
•e-BFe-A = e-(A+BF) = probability of an individual with F being alive.
•ln(Probability of an individual with F being alive) = -A - BF
Consider first a model in which a recessive allele is
lethal when homozygous.
•ln(Probability of an individual with F being alive) = -A - BF
•Because BF>0, the above equation describes inbreeding
depression, the reduction of a beneficial trait (such as viability or
birth weight) with increasing levels of pedigree inbreeding.
•To detect and describe inbreeding depression, pool together all the
animals in a population with the same F to estimate the probability
of being alive, and then regress the ln(prob. of being alive) vs. F.
Inbreeding Depression in Speke’s gazelle
F displays strong interactions with rare, recessive
alleles and epistatic gene complexes.
Example of epistasis: synthetic lethals.
•Knock-out (complete loss of function) mutations were induced for
virtually all of the 6200 genes in the yeast (Saccharomyces
cerevisiae) genome (Tong et al. 2001. Sci. 294:2364-2368).
•>80% of these knock-out mutations were not lethal when made
homozygous through identity by descent and classified
“nonessential”
•Extensive lethality emerged when yeast strains were bred that bore
homozygous pairs of mutants from this “nonessential” class.
•Therefore, B = the number of “lethal equivalents” rather than the
number of actual lethal alleles.
F displays strong interactions with rare, recessive
alleles and epistatic gene complexes.
•2B = the number of lethal equivalents in heterozygous condition
that a living animal is expected to bear.
•For Speke’s gazelles, the average number of lethal equivalents for
one-year survivorship borne by the founding animals of this herd is
therefore 7.5 lethal equivalents per animal.
•Humans from the United States and Europe yield values of 2B
between 5-8.
•Therefore, even small amounts of pedigree inbreeding in a
population may increase the incidence of some types of genetic
disease by orders of magnitude in the pedigree-inbred subset of the
population (e.g., 0.05% of matings in the US are between cousins,
but 18-24% of albinos in the US come from cousin matings vs. an
overall incidence of 0.006%).
System of Mating Inbreeding, f
• F is calculated for individuals from pedigree
data.
• Demes are defined by a shared system of
mating, but this is a population level concept.
• Therefore, we need another definition of
inbreeding at the level of a deme to describe
the population incidence of matings between
relatives.
Inbreeding as a Deviation from Random Mating
A a
Gene Pool
p q = 1-p
Maternal Gamete
A a
p q
A AA Aa
Paternal Gamete
p p2 + pq-
a aA aa
q qp- q2 +
Genotype Frequencies that Deviate
From Random Mating due to
AA Aa aa
p2 + 2pq-2 q2 +
Define f = (pq)
AA Aa aa
p2 +pqf 2pq(1-f) q2 +pqf
Can Estimate f = 1-Freq(Aa)(2pq)
f = panmictic index, but usually
called the “inbreeding coefficient”
• Measures the rules by which gametes unite at the
level of the deme
• Is a measure of system of mating
• Random mating is a special case where f=0
• Inbreeding is a special case where f > 0
• Avoidance of inbreeding is a special case where
f 0, and the average F
= 0.149
• Therefore, this herd of Speke’s Gazelle is One of
the Most Highly Inbred Mammalian Populations
Know.
• A genetic survey in 1982 yielded f = -0.3
• Therefore, this herd of Speke’s Gazelle is a
Mammalian Population That Strongly Avoids
Inbreeding.
• CONTRADICTION?
Inbreeding (F) in a Human Population
Strongly Avoiding Inbreeding (f)
Tristan da Cunha
Impact of f
• Can greatly affect genotype frequencies,
particularly that of homozygotes for rare
alleles: e.g., let q =.001, then q2 = 0.000001
Now let f = 0.01, then q2+pqf = 0.000011
• f is NOT an evolutionary force by itself:
p’ = (1)(p2+pqf) + (.5)[2pq(1-f)]
= p2+pq + pqf - pqf
= p(p+q) = p
A contrast between F, the pedigree inbreeding coefficient,
and f, the system-of-mating inbreeding coefficient
Property F f
Data Used Pedigree Data Genotype
Frequency Data
Type of Measure Probability Correlation
Coefficient
Range 0≤F≤1 -1 ≤ f ≤ 1
Level Individual Deme
Biological Probability of System of
Meaning Identity–by–De- Mating or HW
scent Deviation
Assortative Mating
occurs when individuals with similar
phenotypes are more likely to mate
than expected under random pairing
in the population
Assortative Mating
Reynolds, R. Graham & Fitzpatrick, Benjamin M. Evolution 61 (9), 2253-2259.
100% Assortative Mating For A Codominant, Single Locus Phenotype
AA Aa aa
Zygotes
GAA GAa Gaa
Phenotype 1 1 1
Production
Phenotypes of TAA TAa Taa
Adult Population GAA GAa Gaa
Mate Choice 1 1 1
AA X AA Aa X Aa aa X aa
Mated Adults GAA GAa Gaa
Meiosis & 1 1/ 1/2 1/ 1
4 4
Fertilization
AA Aa aa
Zygotes
GAA+GAa/4 GAa/2 Gaa+GAa/4
100% Assortative Mating For A Codominant, Single Locus Phenotype
AA Aa aa
Zygotes
GAA GAa Gaa
p = (1)GAA+(1/2)GAa 1 1 1
Phenotypes of TAA TAa Taa
Adult Population GAA GAa Gaa
Mate Choice 1 1 1
AA X AA Aa X Aa aa X aa
Mated Adults GAA GAa Gaa
p’ = (1)(GAA+ GAa/4)+(1/2)GAa/2 1 1/ 1/2 1/ 1
4 4
p’ = GAA+ GAa/2 = p
AA Aa aa
Zygotes
GAA+GAa/4 GAa/2 Gaa+GAa/4
100% Assortative Mating For A Codominant, Single Locus Phenotype
AA Aa aa
Zygotes
GAA GAa Gaa
Gen. 0
1 1/ 1/2 1/ 1
4 4
AA Aa aa
Zygotes
Gen. 1
GAA+GAa/4 GAa/2 Gaa+GAa/4
Note, GAa(1)= 1/2GAa(1) => GAa(i)= (1/2)iGAa(0)
As i , GAa(equilibrium) 0
AA aa
At equilibrium: GAA+GAa/2 = p Gaa+GAa/2 = q
Profound, Early Onset Deafness
• Assortative Mating Rates Vary From 80% to 94%
in USA and Europe.
• About half of the cases are due to accidents and
disease
• The other half of the cases are due to
homozygosity for a recessive allele at any one of
35 loci.
• Half of the genetic cases are due to homozygosity
for a recessive allele at the GJB2 locus that
encodes the gap-junction protein connexin-26,
with q 0.01 in European and USA populations.
GJB2 locus, Alleles A and a
• Frequency of a is about 0.01 in U.S.A. &
Europe
• Under random mating expect an aa
genotype frequency of (0.01)2 = 0.0001 who
will be deaf
• Actual incidence of deafness due to aa is
0.0003 to 0.0005 (as if f=0.02 to 0.04)
• 3 to 5 times more children are deaf due to
this gene because of assortative mating.
GJB2 locus, Alleles A and a
• Only a quarter of the people with profound
early onset deafness are aa.
• Within matings of deaf people, therefore
expect (1/4)(1/4) = 1/16 to be aa X aa.
• But 1/6 of the children of deaf couples are aa!
• In many of these couples, one of the parents is
deaf due to homozygosity for a recessive allele
at another locus, yet this person is also Aa at
the GJB2 locus.
GJB2 locus, Alleles A and a
• Consider a second locus with alleles B and b such
that bb is deaf and frequency of b is 0.0001.
• Under random mating equilibrium, expected
frequency of ab gametes is (0.01)(0.0001) =
0.000001
• But assortative mating implies that the rare bb
individuals will mate 1/4 of the time with aa
individuals, and the children of such matings can
produce ab gametes.
• THEREFORE, ASSORTATIVE MATING
CREATES LINKAGE DISEQUILIBRIUM!
2-Locus, 2-Allele 100% Assortative Mating With Additive Phenotypes
Equilibrium Populations Possible Under
a 2-Locus, 2-Allele 100% Assortative
Mating With Additive Phenotypes
Initial Gene Pool
Genotypes pA = pB pA pB
AB/AB pA pA pB
Ab/Ab 0 0 pA - pB
aB/aB 0 pB - pA 0
ab/ab pb pb pa
Note, can start with D=0, but all equilibrium populations have |D’|=1
Properties of Assortative Mating
• Increases the Frequency of Homozygotes Relative to
Hardy-Weinberg For Loci Contributing to the
Phenotype Or For Loci Correlated For Any Reason to
the Phenotype
• Does Not Change Allele Frequencies --Therefore Is Not
An Evolutionary Forces at the Single Locus Level
• Assortative Mating Creates Disequilibrium Among Loci
that Contribute to the Phenotype and Is A Powerful
Evolutionary Force at the Multi-Locus Level
• Multiple Equilibria Exist at the Multi-Locus Level And
The Course of Evolution Is Constrained By the Initial
Gene Pool: historical factors are a determinant of the
course of evolution
Assortative Mating & Inbreeding
• Both Increase Frequency of Homozygotes Relative to
Hardy-Weinberg (result in f > 0)
• Increased Homozygosity Under Assortative Mating
Occurs Only For Loci Contributing to the Phenotype Or
For Loci Correlated For Any Reason to the Phenotype;
Inbreeding Increases Homozygosity for All Loci
• Neither Changes Allele Frequencies --Therefore They
Are Not Evolutionary Forces at the Single Locus Level
• Assortative Mating Creates Disequilibrium Among Loci
that Contribute to the Phenotype; Inbreeding Slows
Down the Decay of Disequilibrium, but Does Not
Create Any Disequilibrium.
ASSORTATIVE MATING, LINKAGE
DISEQUILIBRIUM AND ADMIXTURE
• Assortative mating directly affects the genotype and
gamete frequencies of the loci that contribute to the
phenotype for which assortative mating is occurring and
of any loci in linkage disequilibrium with them.
• Admixture occurs when two or more genetically
distinct subpopulations are mixed together and begin
interbreeding.
• Admixture induces disequilibrium, so assortative mating
for any phenotype associated with the parental
subpopulations can potentially affect the genotype
frequencies at many loci not directly affect the assorting
phenotype.
ASSORTATIVE MATING, LINKAGE
DISEQUILIBRIUM AND ADMIXTURE
Subpopulation 1 Subpopulation 2
AB Ab aB ab AB Ab aB ab
0.03 0.07 0.27 0.63 0.63 0.27 0.07 0.03
D = (0.03)(0.63)-(0.07)(0.27) = 0 D = (0.63)(0.03)-(0.27)(0.07) = 0
Combined Population (50:50 Mix)
AB Ab aB ab
0.33 0.17 0.17 0.33
D = (0.33)(0.33)-(0.17)(0.17) = 0.08
ASSORTATIVE MATING, LINKAGE
DISEQUILIBRIUM AND ADMIXTURE
• Assortative mating for any trait that differentiates the
original subpopulations (even non genetic) reduces
heterozygosity at all loci with allele frequency
differences between the original subpopulations.
• The rate of dissipation of D in the admixed population is
therefore (1-r).
• Therefore, disassortative mating rapidly destroys
the linkage disequilibrium created by admixture.
Disassortative Mating and Admixture
Disassortative Mating and Admixture
Diagnostic Yanomama Borabuk Makiritare
Allele Yanomama
Dia 0.00 0.06 0.04
Apa 0.00 0.08 0.05
Systems of Matings
Systems of mating can be potent
evolutionary forces, both by
themselves and in interactions with
other evolutionary factors. In
subsequent lectures we will examine
additional interactions between
system of mating and other
evolutionary forces.