Spring 2010 by HC11121306045

VIEWS: 10 PAGES: 2

									         BIO 2420: Introduction to Microbiology
                                                  Spring 2010
     Lab Text (Required): Microbiology Laboratory Theory and Application 2 nd Edition, Pierce & Leboffe

Week 1    Jan 20   ...................................................... Introduction to Lab and Microorganisms
                   Ex. 2-1 .............................................................. Ubiquity of Microorganisms ............. p. 28
                   Ex. 2-15 ...................................................... Effectiveness of Handscrubbing ............. Handout

Week 2    Jan 25   ...................................................... Lab Familiarization & Safety Guidelines ............. Handout

          Jan 27   Ex. 1-2 ............................................................................... Aseptic Transfers ............. p. 14
                   Ex. 2-4 ................................................................... Growth Patterns in Broth ............. p. 39

Week 3    Feb 1    Ex. 1-3 ........................................................Streak Plate Method of Isolation ............. p. 20
                   Ex. 2-2 ......................................................................Cultural Characteristics ............. p. 30

          Feb 3    Ex. 3-1 ................................................ Introduction to the Light Microscope ............. p. 64
                   Ex. 3-3 ................................................ Examination of Eukaryotic Microbes ............. p. 72
                   Ex. 3-10 ....................................................................................... Wet Mount ............. p. 100

Week 4    Feb 8    Ex. 3-4 ..................................................................................... Simple Stains ............. p. 81
                   Ex. 3-5 ....................................................................................Negative Stain ............. p. 84

          Feb 10   Ex. 3-6 ......................................................................................... Gram Stain ............. p. 86

Week 5    Feb 15   Ex. 3-7 ..................................................... Acid-Fast Stains (prepared slides) ............. p. 91
                   Ex. 3-8 ..........................................................Capsule Stain (prepared slides) ............. p. 95
                   Ex. 3-9 ..................................................... Endospore Stain (prepared slides) ............. p. 97
                   Ex. 3-11 .......................................................Flagella Stain (prepared slides) ............. p. 102

          Feb 17   Ex. 5-28 .................................................................................... Motility Test ............. p. 198
                   Ex. 3-12 .................................................... Begin Morphological Unknown ............. p. 104

Week 6    Feb 22   ............................................................................................. PRACTICAL I
                   ................................................................Continue Morphological Unknown

          Feb 24   Ex. 5-13 .............................................................................Starch Hydrolysis ............. p. 161
                   Ex. 5-16 .................................................................................... Casease Test ............. p. 169
                   Ex. 5-17 .................................................................................Gelatinase Test ............. p. 171
                   Ex. 5-18 ...................................................................................... DNase Test ............. p. 173
                   Ex. 5-19 ....................................................................................... Lipase Test ............. p. 175
                   ................................................................Continue Morphological Unknown

Week 7    Mar 1    Ex. 5-2 ............................................................................... Phenol Red Broth ............. p. 131
                   Ex. 5-4 ............................................ Methyl Red and Voges-Proskauer Tests ............. p. 136
                   ................................................................Continue Morphological Unknown

          Mar 3    Ex. 2-8 ............................................................ Fluid Thioglycollate Medium ............. p. 45
                   Ex. 2-9 ..................................................................................... Anaerobic Jar ............. p. 47
                   Ex. 5-7 ....................................................................... Nitrate Reduction Test ............. p. 145


            For lab information, pictures, practice quizzes, definitions, and more,
                           http://www.austincc.edu/microbugz/
Week 8     Mar 8      Ex. 5-5 ...................................................................................... Catalase Test ............. p. 141
                      Ex. 5-6 ...................................................................................... Oxidase Test ............. p. 143
                      ..................................................................... Morphological Unknown Due

           Mar 10     ........................................................................................... PRACTICAL II


                                               * * * Spring Break * * *
_______________________________________________________________________________________________________________________

Week 9     Mar 22     Ex. 5-8 ........................................................................................ Citrate Test ............. p. 149
                      Ex. 5-10 ....................................................................... Decarboxylation Test ............. p. 153
                      Ex. 5-11 ........................................................ Phenylalanine Deaminase Test ............. p. 156
                      Ex. 5-15 ........................................................................ Urease Tests (Broth) ............. p. 166

           Mar 24     Combination media:
                      Ex. 5-20 ....................................................................................SIM Medium ............. p. 177
                      Ex. 5-21 .....................................................................Triple Sugar Iron Agar ............. p. 181
Week 10    Mar 29     Selective and Differential Media for G+ Organisms:
                      Ex. 4-1 ............................................................................ Mannitol Salt Agar ............. p. 108
                      Ex. 4-2 ................................................................. Phenylethyl Alcohol Agar ............. p. 111
                      Ex. 5-27 .................................................................. Coagulase Tests (Demo) ............. p. 196

           Mar 31     ............................................................................... 6.5% Salt Tolerance Test ............. Handout
                      Ex. 5-12 .............................................................................. Bile Esculin Test ............. p. 158
                      Ex. 5-24 ............ Bacitracin Susceptibility Test (and Optochin; A &P Disks) ............. p. 189
                      Ex. 5-26 ...................................................................................... Blood Agar ............. p. 193

Week 11    Apr 5      Selective and Differential Media for G- Organisms:
                      Ex. 4-5 ............................................................... Eosin Methylene Blue Agar ............. p. 118
                      Ex. 4-6 ........................................................................ Hektoen Enteric Agar ............. p. 120
                      Ex. 4-7 ............................................................................... MacConkey Agar ............. p. 122

           Apr 7      Ex. 5-31 .................................................................. Begin Mixed Unknown ............. p. 212

Week 12    Apr12      .......................................................................................... PRACTICAL III
                      ............................................................................. Continue Mixed Unknown

           Apr 14     ............................................................................. Continue Mixed Unknown

Week 13    Apr 19     Ex. 7-3 ................. Antimicrobial Susceptibility Test (Kirby-Bauer Method) ............. p. 242
                      ............................................................................. Continue Mixed Unknown

           Apr 21     .................................................................................................. Disinfectants ............. Handout
                      ............................................................................. Continue Mixed Unknown

Week 14    Apr 26     Ex. 2-13 ....................................... Ultraviolet Radiation Damage and Repair ............. p. 55
                      ............................................................................. Continue Mixed Unknown

           Apr 28     Ex. 11-1 .........................................The Fungi—Common Yeasts and Molds ............. p. 312
                      ............................................................................. Continue Mixed Unknown

Week 15    May 3      Ex. 11-2 ......... Examination of Common Protozoans of Clinical Importance ............. p. 320
                      ............................................................................. Continue Mixed Unknown

           May 5      Ex. 11-3 ......................................................................................................... ............. Helminth
                      Parasites, Nematode Parasites, Hookworms ........................................ p. 327

                      ................................................................................... Mixed Unknown Due

Week 16    May 10     .......................................................................................... PRACTICAL IV

           May 12     ............................................................................. LAB DOES NOT MEET

								
To top