CERTIFICATE IN LABORATORY MEDICAL TECHNOLOGY by liaoqinmei

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									BANGLADESH TECHNICAL EDUCATION BOARD
       AGARGAON, DHAKA-1207




 CERTIFICATE-IN-MEDICAL TECHNOLOGY

             SYLLABUS OF


 CERTIFICATE-IN-LABORATORY MEDICINE
            TECHNOLOGY
SL.   Subject          Name of the subject                                          MARKS
      code
No                                                                    Theory         Practical      Total
                                                  T   P    C        Cont. Final    Cont.    Final
                                                                    assess exam.   assess   exam.
 1       1321   Hematology                    2       6        4      50     50      60       40    200
 2       1322   Immunology & Hormonal Assay   2       6        4     50     50      60       40     200
 3       1323   Special Microbiology          2       6        4     50     50      60       40     200
 4       1324   Histopathology                2       3        3      50    50       30      20     150
 5       1325   Biochemistry                  2       6        4      50    50       60      40     200
 6.      7526   Field Training                0       6        2       -     -       60      40     100
                                              10      33       21    250   250      330     220     1050
                                 Total
                                         Hematology
                                       Sub-Code:-1321
                                                                            T       P        C
                                                                            2       6        4

Objective: At the end of the course the student will be able to:
   1. Demonstrate knowledge on functions and composition of blood.
   2. Explain the development, functions and normal values of blood cells and the
       variations in morphology in abnormal situation.
   3. Identify, use and take care of different instruments/equipments/apparatus used in a
       hematological laboratory.
   4. Do the steps for collection of capillary/venous/arterial blood
   5. Do the correct use of anticoagulants in a hematological laboratory
   6. Extract serum and plasma from whole blood.
   7. Follow principles & procedure for estimation of hemoglobin & total count of RBC.
   8. Follow the principles and perform the procedure for estimation of PCV, MCV, MCH
       and MCHC.
   9. Follow the principles and perform the procedure for estimation of doing total counts
       of WBC and platelets and differential count of WBC.
   10. Follow the principles and procedure for measurement of ESR, Volume index, Colour
       index, Blood and plasma volume.
   11. Follow the principles and perform the procedure for determining of BT, CT, PT,
       Calcium time, Clot retraction time & fragility of RBC’s
   12. Follow the principles and perform the technique of obtaining bone marrow for
       hematological examination.
   13. Carry out laboratory methods for identification of malarial parasites & filarial in
       blood.

 SL. No      Topics/Lessons                                                Theory   Practical
    1     Introduction to Hematology                                         04         03
           ▫ Definition, composition and function of blood.
           ▫ Blood cells: formation, function, fate and normal values of
              different blood cells
           ▫ Normal and abnormal blood cells
           ▫ Apparatus used for examination of blood
           ▫ Methods for cleaning apparatus
           ▫ Methods for collecting & preservation of capillary
              blood/venous blood
           ▫  Methods of using oxalated blood
           ▫  Techniques for separation of serum & plasma
           ▫  Anticoagulants used in the hematological laboratory
           ▫  Anaemia: Definition and classification
    2     Hemoglobin                                                         02         03
           ▫ Definition and normal values
           ▫ Methods of hemoglobin estimation (Sahli’s &
             Cyanmethhemoglobin method)
           ▫ Method for hemoglobin electrophoresis
    Blood cell counting
     ▫ Thin and thick blood films preparation                      05
                                                                        04
     ▫ Counting Red Blood Cells: Principles & procedures
    ▫ Methods and procedure of total leukocytes count
    ▫ Methods and procedure of differential count of WBC
    ▫ Methods and procedure of counting Reticulocytes
     ▫ Methods and procedure of Platelets
3   Principles/methods and procedures for determining
    ▫ Packed cell Volume (PCV)
    ▫ Mean Corpuscular Volume (MCV)                                04
    ▫ Mean Corpuscular Hemoglobin (MCH)                                 05
    ▫ Mean Corpuscular Hemoglobin Concentration (MCHC)
    ▫ Erythrocyte Sedimentation Rate (ESR)
4   Principles and procedures for determining
    ▫ Coagulation Time (CT)                                        05
                                                                        07
    ▫ Bleeding Time (BT)
    ▫ Prothrombin Time (PT)
    ▫ Clot Retraction Time
    ▫ Fragility of RBC’s
5   Principles and technique of obtaining bone marrow for          02
                                                                        01
    examination
6   Principles and procedures for identification of malarial and   02
    filarial parasites in blood, leishmania donovari body               01
                                                                   24   24
    Total
                             Immunology & Hormonal Assay
                                   Sub-Code:-1322
                                                                               T        P    C
                                                                               2        6    4
Objective: At the end of the course the student will be able to:
   1. Demonstrate knowledge on the principles of immunity and the factors affecting it.
   2. Apply the principles of application of basic immunological Techniques in diagnosing
      microbial diseases and hormonal disorders.
   3. Perform the procedures for immunological tests.
   4. Carry out the methods of hormone assay for facilitating detection of certain
      hormones.
Course Contents
 SL No    Topic/Lesson                                                         Theory Practical
                                                                                Class  Class
    1     Immunology                                                             14      14
          ▫ Definition
          Immunity
          ▫ Definition & classification
          ▫ Major components of immunity system
          ▫ Short description of different types of immunity
          ▫ Factors affecting immunity
          Antigen (Ag)
          ▫ Definition
          Antibody (Ab)
          ▫ Definition & classification
          ▫ Difference between T-Lymphocyte & B-Lymphocyte
          ▫ Auto-immune disease
          ▫ Immunodeficiency disorders
    2     Hormone Assay:                                                           10       10
          ▫ Principles and methods
          ▫ Thyroid function tests
          ▫ Assay of T3, T4, TSH: Indication, methods & procedure,
          ▫ Prolactin: source, normal range, indication & procedure of assay
          ▫ Oestrogen: source, normal range, indication & procedure of assay
          ▫ Progesterone: source, normal range, indication & procedure of
            assay
          ▫ Testosterone: source, normal range, indication & procedure of
            assay
          ▫ Follicle Stimulating Hormone (FSH): source, normal range,
            indication & procedure of assay
          ▫ hCG: source, normal range, indication & procedure of assay of ß-
            hCG
          ▫ List the name of important hormones & produce of assay
          Factors that influence the use of serological tests

                                                                                   24       24
          Total
                                         Special Microbiology
                                           Sub-Code:-1323
                                                                                     T      P        C
                                                                                     2      6        4
Objective: At the end of the course the student will be able to:

       1. Demonstrate an adequate knowledge base on different aspects of microbiology related
          to pathogenecity and antigenecity of bacteria of medical importance.
       2. Understand operational safety of microbiology laboratory.
       3. Learn how to operate, use and maintain important equipment and apparatuses.
       4. Collect, transport and process the specimens for microbiological examinations.
       5. Perform a biochemical testing of microorganism.
Course contents
 Sl.          Topics/Lessons                                                      Theory    Practical
                                                                                   No of      No of
 No
                                                                                  classes    classes
   1       Special Bacteriology                                                     06         04
           ▫ Classification, staining reaction, cultural characteristics of the
              following group of bacteria.
           Gram positive and negative cocci and bacilli-Staphylococcus,
           Streptococcus, Pneumococcus, Gonococcus, Mycobacterium,
           Corrynebacterium, Salmonella, shigella, Eschericia, Klebsiella,
           Vibrio, Clostridium, Spirochetes, Pseudomonas
   2       Virology                                                                 03          03
           ▫ classification, characteristics and transmission of the
              medically important viruses
           ▫ Viral infection
           ▫ Collection and transportation of specimen of virus
           ▫ Laboratory diagnosis of virus
   3.      Microscopical examination of bacteriological specimen:                   03          05
           ▫ Unstained preparation
           ▫ Stained preparation-Gram staining AFB, Albert, Giemsa’s
              Loefler’s Methylene Blue and Hiss staining methods
   4.      Culture of microorganism:                                                04          06
           ▫ Classification of Media
           ▫ Preparation of important media-Nutrient broth, Nutrient agar,
              Blood agar, chocolate agar, Mckonky’s agar, Loefler’s Serum
              Slope, Robertson’s cooked meat media, Loenstein’s jensen
              media and Monsur’s Media.
   5.      Inoculation and incubation of culture media                              03          04
            ▫ Study of colonies
            ▫ Isolation and identification of bacteria
            ▫ Culture of Throat swab, urine, stool, blood, pus, sputum &
               vaginal swab
   6.      Quality assurance in culturing microorganism                             03          01
            ▫ Areas requiring quality control
            ▫ Quality control of collection of specimens and transport
            ▫ Reporting and recording results
   7.      Procedure for laboratory diagnosis of fungus of medical                  02          01
           importance: Fresh examination of specimen for dermatophytes.
   8.      Urine: Specimen collection & preservation
           - Routine & M/E
     - Benedict test
     - Heat coagulation test
9.   Stool: Routine & M/E
           Occult blood test
                               24   24
     Total
                                        Histopathology
                                        Sub-Code:-1324
                                                                               T   P        C
                                                                               2   3        3

Objective: At the end of the course the student will be able to:

   1. Differentiate normal and abnormal tissues of human body.
   2. Describe the function, operation and use of histopathological equipment,
      apparatus/glassware and chemicals
   3. Identify histopathological specimen and collect, Transport and preserve the same.
   4. Prepare and use properly histopathological chemicals and reagents.

Course Contents
 Sl. No         Topics/Lessons                                          Theory     Practical
    1     Pathological change of tissue in different Chemicals            03           01
          condition
    2     Classification and gross identification of                      03           -
          histopathological specimens
   3.     Collection, transport and preservation of                       03           -
          histopathological specimens
   4.     Principles and methods of paraffin fixation, block              04           06
          preparation, section, cutting, shade predation and staining
          and mounting for histopathological examination
   5.     Function, operation and use of histopathological                07           05
          equipments, apparatus, glass wares:
            ▫ Microtomy
            ▫ Paraffin Bath/Water bath/Hot Air Oven
            ▫ Incubator/Block Capsule/Wax/Refrigerator
            ▫ Coplin jar/Specimen jars Slides/Cover Slides
            ▫ Mounting gum/Diamond pencil (Marker)/Sharpening
              Stone
   6.     Preparation and use of histopathological chemicals and          04           -
          reagents.
                                                                          24           12
          Total
                                 Biochemistry & Serology
                                     Sub-Code:-1325
                                                                           T      P        C
                                                                           2      6        4
Objective: At the end of the course the student will be able to:
1. Understand biochemical properties of human body.
2. Perform common biochemical and serological tests
                                        Course Contents
  Sl.                          Topics                            Theory         Practical
  No.                                                          No of classes   No of classes
  1.      Biochemistry: Introduction and importance                 01               -
  2.      Lipids: Definition, classification, normal values,        02              02
          procedure of examination of lipid profile
   3.     Enzymes: Definition, classification, properties,          02                02
          diagnostic importance
          Amylase: Lipase, definition, normal value,
          procedure of examination in blood/urine
   4.     Body Fluid: Definition, composition, difference           02                -
          between I.C.F & E.C.F., water balance, sources
          of water in the body, causes of water loss from
          the body.
   5.     Organ function test: Function of kidney, Renal            06                06
          function test (serum creatinine and urea),
          Function of liver, Liver function test (serum
          bilirubin, SGPT, SGOT, Serum alkaline
          phosphatase) Thyroid function test.
   6.     Clinical biochemical & serological tests:                 11                14
          Indication, Normal value, Procedure of
          examination of –
              - Blood sugar: Random, Fasting, PPBS,
                  2hr after breakfast, GTT
              - TPHA
              - VDRL
              - ASO Titre
              - RA test
              - HBsAg
              - Widal test
              - Blood grouping: ABO, Rh
              - PSA
              - Fasting lipid profile
              - S. bilirubin
              - SGPT
              - SGDT
              - Alkaline phosphatase
              - Blood urea
              - S. creatinine
                             Total                                  24                24
Filed Training

								
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